检索结果-南通市图书馆

Evolution of the newest diagnostic methods for COVID-19:a Chinese perspective

在线全文

学校读者我要写书评

暂无评论

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2023年第6期24卷 463-484页

作者： Mingtao LIU Jiali LYU Xianhui ZHENG Zhiman LIANG Baoying LEI Huihuang CHEN Yiyin MAI Huimin HUANG Baoqing SUN Department of Clinical laboratory National Center for Respiratory MedicineNational Clinical Research Center for Respiratory DiseaseState Key Laboratory of Respiratory DiseaseGuangzhou Institute of Respiratory Healththe First Affiliated Hospital of Guangzhou Medical UniversityGuangzhou510140China National Center for respiratory Medicine National Clinical Research Center of Respiratory DiseaseState Key Laboratory of Respiratory DiseaseGuangzhou Institute of Respiratory Healththe First Affiliated Hospital of Guangzhou Medical UniversityGuangzhou 510140China Guangzhou Medical University Guangzhou 511495China

Coronavirus disease 2019(COVID-19)has continued to spread globally since late 2019,representing a formidable challenge to the world's healthcare systems,wreaking havoc,and spreading rapidly through human contact.With fever,fatigue,and a persistent dry cough being the hallmark symptoms,this disease threatened to destabilize the delicate balance of our global community.Rapid and accurate diagnosis of COVID-19 is a prerequisite for understanding the number of confirmed cases in the world or a region,and an important factor in epidemic assessment and the development of control measures.It also plays a crucial role in ensuring that patients receive the appropriate medical treatment,leading to optimal patient care.Reverse transcription-polymerase chain reaction(RT-PCR)technology is currently the most mature method for detecting viral nucleic acids,but it has many drawbacks.Meanwhile,a variety of COVID-19 detection methods,including molecular biological diagnostic,immunodiagnostic,imaging,and artificial intelligence methods have been developed and applied in clinical practice to meet diverse scenarios and needs.These methods can help clinicians diagnose and treat COVID-19 patients.This review describes the variety of such methods used in China,providing an important reference in the field of the clinical diagnosis of COVID-19.

关键词： Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) Coronavirus disease 2019(COVID-19) Diagnosis Polymerase chain reaction(PCR) Immunoassay Radiography

Eosinophil-derived chemokine(hCCL 15/23,mCCL6)interacts with CCR1 to promote eosinophilic airway inflammation

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Signal Transduction and Targeted Therapy 2021年第3期6卷 1046-1056页

作者： Xufei Du Fei Li Chao Zhang Na Li Huaqiong Huang Zhehua Shao Min Zhang Xueqin Zhan Yicheng He Zhenyu Ju Wen Li Zhihua Chen Songmin Ying Huahao Shen key laboratory of respiratory disease of Zhejiang Province Department of Respiratory and Critical Care Medicinethe Second Affiliated Hospital of Zhejiang University School of MedicineHangzhou 310009China Department of Anatomy Zhejiang University School of MedicineHangzhou 310058China key laboratory of Regenerative Medicine of Ministry of Education Institute of Aging and Regenerative MedicineJinan UniversityGuangzhouGuangdong 510632China International Institutes of Medicine Zhejiang University School of MedicineYiwu 322000China Department of Pharmacology Zhejiang University School of MedicineHangzhou 310058China State Key Lab of Respiratory Disease Guangzhou 510120China

Eosinophils are terminally differentiated cells derived from hematopoietic stem cells(HSCs)in the bone marrow.Several studies have confirmed the effective roles of eosinophils in asthmatic airway pathogenesis.However,their regulatory functions have not been well elucidated.Here,increased C-C chemokine ligand 6(CCL6)in asthmatic mice and the human orthologs CCL15 and CCL23 that are highly expressed in asthma patients are described,which are mainly derived from eosinophils.Using Cc/6 knockout mice,further studies revealed CCL6-dependent allergic airway inflammation and committed eosinophilia in the bone marrow following ovalbumin(OVA)challenge and identified a CCL6-CCR1 regulatory axis in hematopoietic stem cells(HSCs).Eosinophil differentiation and airway inflammation were remarkably decreased by the specific CCR1 antagonist BX471.Thus,the study identifies that the CCL6-CCR1 axis is involved in the crosstalk between eosinophils and HSCs during the development of allergic airway inflammation,which also reveals a potential therapeutic strategy for targeting G protein-coupled receptors(GPCRs)for future clinical treatment of asthma.

关键词： CCR1 inflammation airway

The Effects of Secondary Pneumonia on the Curative Efficacy of Multidrug-resistant Tuberculosis: A Retrospective Cohort Study

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2018年第12期31卷 908-912页

作者： TAN Shou Yong LIANG Zhuo Zhi Gift Chiwala KUANG Hao Bin HUANG Zhi Pei QIN Hong Juan LI Yan LI Yan Qiong H.M.Adnan Hameed ZHANG Tian Yu state key laboratory of respiratory disease Guangzhou Chest Hospital Guangzhou 510095 Guangdong China School of Public Health Sun Yat-sen University Guangzhou 510080 Guangdong China state key laboratory of respiratory disease Guangzhou Institutes of Biomedicine and Health Chinese Academy of Sciences Guangzhou 510530 Guangdong China University of Chinese Academy of Sciences Beijing 100049 China

Tuberculosis(TB)is a formidable global health problem and ranks above HIV as the leading cause of death world wide.In2017,a total number of 10.0 million cases of TB were reported, which resulted in1.3 million TB death... 详细信息

关键词： TB In MDR A Retrospective Cohort Study

FGFR/RACK1 interacts with MDM2, promotes P53 degradation, and inhibits cell senescence in lung squamous cell carcinoma

在线全文

学校读者我要写书评

暂无评论

Cancer Biology & Medicine 2021年第3期18卷 665-674页

作者： Tao Chen Fei Wang Shupei Wei Yingjie Nie Xiaotao Zheng Yu Deng Xubin Zhu Yuezhen Deng Nanshan Zhong Chengzhi Zhou state key laboratory of respiratory disease National Clinical Research Center for Respiratory DiseaseGuangzhou Institute of Respiratory Healththe First Affiliated Hospital of Guangzhou Medical UniversityGuangzhou 510120China NHC key laboratory of Pulmonary Immunological diseases Clinical Research Lab CenterGuizhou Provincial People’s HospitalGuiyang OK 550002China Longgang Central Hospital of Shenzhen Affiliated Shenzhen Longgang Central Hospital of Zunyi Medical CollegeShenzhen 518116China Center for Molecular Medicine Xiangya HospitalCentral South UniversityChangsha 410078China

Objective:FGFR is considered an important driver gene of lung squamous cell carcinoma(LSCC).Thus,identification of the biological events downstream of FGFR is important for the treatment of this malignancy.Our previous study has shown that the FGFR/RACK1 complex interacts with PKM2 and consequently promotes glycolysis in LSCC cells.However,the biological functions of the FGFR/RACK1 complex remain poorly understood.Methods:Anchorage-independent assays and in vivo tumorigenesis assays were performed to evaluate cancer cell malignancy.Distant seeding assays were performed to evaluate cancer cell metastasis.β-gal staining was used to examine cell senescence,and immunoprecipitation assays were performed to examine the interactions among FGFR,RACK1,and MDM2.Results:FGFR/RACK1 was found to regulate the senescence of LSCC cells.Treatment with PD166866,an inhibitor of FGFR,or knockdown of RACK1 induced senescence in LSCC cells(P<0.01).A molecular mechanistic study showed that FGFR/RACK1/MDM2 form a complex that promotes the degradation of p53 and thus inhibits cell senescence.PD166866 and RG7112,an MDM2/p53 inhibitor,cooperatively inhibited the colony formation and distal seeding of LSCC cells(P<0.01),and upregulated the expression of p53 and p21.Conclusions:Together,our findings revealed the regulatory roles and mechanisms of FGFR/RACK1 in cell senescence.This understanding should be important in the treatment of LSCC.

关键词： FGFR RACK1 MDM2 P53 senescence

A zwitterionic polymer-inspired material mediated efficient CRISPR-Cas9 gene editing

在线全文

学校读者我要写书评

暂无评论

Asian Journal of Pharmaceutical Sciences 2022年第5期17卷 666-678页

作者： Lingmin Zhang Langyu Yang Jionghua Huang Sheng Chen Chuangjia Huang Yinshan Lin Ao Shen ZhouYikang Zheng Wenfu Zheng Shunqing Tang key laboratory of Molecular Target&Clinical Pharmacology and the state&NMPA key laboratory of respiratory disease School of Pharmaceutical Sciences&The Third and Fifth Affiliated HospitalGuangzhou Medical UniversityGuangzhou 511436China Department of Biomedical Engineering Jinan UniversityGuangzhou 510632China CAS key lab for Biological Effects of Nanomaterials and Nanosafety National Center for NanoScience and TechnologyBeijing 100190China

The typeⅡ prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 (CRISPR/Cas9) adaptive immune system is a cutting-edge genome-editing toolbox.However,its applications are still limited by its inefficient transduction.Herein,we present a novel gene vector,the zwitterionic polymer-inspired material with branched structure (ZEBRA) for efficient CRISPR/Cas9 delivery.Polo-like kinase 1 (PLK1) acts as a master regulator of mitosis and overexpresses in multiple tumor cells.The Cas9 and single guide sgRNA (sgRNA)-encoded plasmid was transduced to knockout Plk1 gene,which was expected to inhibit the expression of PLK1.Our studies demonstrated that ZEBRA enabled to transduce the CRISPR/Cas9 system with large size into the cells efficiently.The transduction with ZEBRA was cell line dependent,which showed~10-fold higher in CD44-positive cancer cell lines compared with CD44-negative ones.Furthermore,ZEBRA induced highlevel expression of Cas9 proteins by the delivery of CRISPR/Cas9 and efficient gene editing of Plk1 gene,and inhibited the tumor cell growth significantly.This zwitterionic polymerinspired material is an effective and targeted gene delivery vector and further studies are required to explore its potential in gene delivery applications.

关键词： CRISPR/Cas9 Gene editing Zwitterionic polymers CD44 PLK1

在线全文

学校读者我要写书评

暂无评论

中国抗击新型冠状病毒的策略与进展

Engineering 2020年第10期6卷 1214-1224页

作者：刘薇关伟杰钟南山 state key laboratory of respiratory disease National Clinical Research Center for Respiratory DiseaseGuangzhou Institute of Respiratory HealthThe First Affiliated Hospital of Guangzhou Medical UniversityGuangzhou Medical UniversityGuangzhou 510120China

新型冠状病毒肺炎(COVID-19)是21世纪第三次由冠状病毒引发的流行病,并导致全球大规模感染和死亡,造成全球性公共卫生事件。基于在防控重症急性呼吸综合征(SARS)和中东呼吸综合征(M ERS)两次疫情中积累的经验,中国迅速确定病毒传播途径... 详细信息

新型冠状病毒肺炎(COVID-19)是21世纪第三次由冠状病毒引发的流行病,并导致全球大规模感染和死亡,造成全球性公共卫生事件。基于在防控重症急性呼吸综合征(SARS)和中东呼吸综合征(M ERS)两次疫情中积累的经验,中国迅速确定病毒传播途径,总结临床特点,提出治疗干预措施并开展疫苗研发,在科学界发挥了关键作用。尽管针对COVID-19的研究取得了快速进展,但在追溯病毒起源、明确传播途径和解析致病机制等方面仍有待进一步研究。目前仍需建立更具针对性的临床治疗方案以及研发特效药。本文总结了中国针对COVID-19防控的主要策略及研究进展,以期为全球疫情防控提供有用的信息。

关键词：新型冠状病毒肺炎血管紧张素转换酶免疫反应炎症临床特点治疗疫苗

UniBind:a novel artificial intelligence-based prediction model for SARS-CoV-2 infectivity and variant evolution

在线全文

学校读者我要写书评

暂无评论

Signal Transduction and Targeted Therapy 2024年第1期9卷 14-15页

作者： Qihong Yan Jincun Zhao state key laboratory of respiratory disease National Clinical Research Center for Respiratory DiseaseGuangzhou Institute of Respiratory Healththe First Affiliated Hospital of Guangzhou Medical UniversityGuangzhouChina

In a recent study published in Nature Medicine,Wang et al.developed an excellent framework called UniBind based on artificial intelligence(AI),which enables accurately predicting infectivity of SARS-CoV-2 variants and evolutionary trends of future viral variants.1 This computational method holds the possibility to not only serve as a valuable early-warning tool for monitoring potential pathogenic SARS-CoV-2 variants but also facilitate fundamental research on protein-protein interactions(PPIs).

关键词： artificial Bind prediction

A diagnostic prediction model for hypertension in Han and Yugur population from the China National Health Survey (CNHS)

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Medical Journal 2023年第9期136卷 1057-1066页

作者： Chengdong Yu Xiaolan Ren Ze Cui Li Pan Hongjun Zhao Jixin Sun Ye Wang Lijun Chang Yajing Cao Huijing He Jin’en Xi Ling Zhang Guangliang Shan Department of Epidemiology and Statistics Institute of Basic Medical SciencesChinese Academy of Medical SciencesSchool of Basic MedicinePeking Union Medical CollegeBeijing 100005China Institute of Chronic and Noncommunicable disease Control and Prevention Gansu Provincial Centre for Disease Control and PreventionLanzhouGansu 730000China Hebei Provincial Center for disease Control and Prevention ShijiazhuangHebei 050000China The State Key Lab of Respiratory Disease The First Affiliated HospitalThe School of Public HealthGuangzhou Medical UniversityGuangzhouGuangdong 510182China Department of Epidemiology and Health Statistics School of Public HealthCapital Medical UniversityBeijing 100069China

Background:The prevalence of hypertension is high among Chinese adults,thus,identifying non-hypertensive individuals at high risk for intervention will help to improve the efficiency of primary prevention strategies.Methods:The cross-sectional data on 9699 participants aged 20 to 80 years were collected from the China National Health Survey in Gansu and Hebei provinces in 2016 to 2017,and they were nonrandomly split into the training set and validation set based on location.Multivariable logistic regression analysis was performed to develop the diagnostic prediction model,which was presented as a nomogram and a website with risk classification.Predictive performances of the model were evaluated using discrimination and calibration,and were further compared with a previously published model.Decision curve analysis was used to calculate the standardized net benefit for assessing the clinical usefulness of the model.Results:The Lasso regression analysis identified the significant predictors of hypertension in the training set,and a diagnostic model was developed using logistic regression.A nomogram with risk classification was constructed to visualize the model,and a website(https://chris-yu.***.io/hypertension_risk_prediction/)was developed to calculate the exact probabilities of hypertension.The model showed good discrimination and calibration,with the C-index of 0.789(95%confidence interval[CI]:0.768,0.810)through internal validation and 0.829(95%CI:0.816,0.842)through external validation.Decision curve analysis demonstrated that the model was clinically useful.The model had a higher area under receiver operating characteristic curves in training and validation sets compared with a previously published diagnostic model based on Northern China population.Conclusion:This study developed and validated a diagnostic model for hypertension prediction in Gansu Province.A nomogram and a website were developed to make the model conveniently used to facilitate the individualized prediction of hypertension in the gene

关键词： Hypertension Diagnostic model Decision curve Yugur people The China National Health Survey

A pair of SARS-CoV-2 nucleocapsid protein monoclonal antibodies shows high specificity and sensitivity for diagnosis

在线全文

学校读者我要写书评

暂无评论

Virologica Sinica 2022年第6期37卷 942-945页

作者： An-Kai Zhu Sa-Sa Li Shu-Pei Yu Zhao-Yong Zhang Huan Li Jing-Feng Li Shan-Shan Gao Heng Chen Jincun Zhao Run Shi Mingjian Lu Chaoyang Li Affiliated Cancer Hospital and Institute of Guangzhou Medical University State Key Laboratory of Respiratory DiseaseKey Laboratory for Cell Homeostasis and Cancer Research of Guangdong High Education InstituteGuangzhou510095China state key laboratory of respiratory disease National Clinical Research Center for Respiratory DiseaseGuangzhou Institute of Respiratory HealthThe First Affiliated Hospital of Guangzhou Medical UniversityGuangzhou510182China Department of Interventional Radiology Affiliated Cancer Hospital and Institute of Guangzhou Medical UniversityGuangzhou510095China

Dear Editor,Severe Acute respiratory Syndrome Coronavirus Type 2(SARS-CoV-2) is the pathogen of the coronavirus disease 2019(COVID-19), which has spread worldwide.SARS-CoV-2 is an enveloped virus belongs to the family of β-coronavirus(Xu et al., 2020). Its genome is a linear single-stranded positive-sense RNA about 30,000 nucleotides packed inside a virion with a diameter of 100 nm and a volume of about 10^(6)nm^(3)(Bar-On et al., 2020).

关键词： al. diagnosis specificity