检索结果-南通市图书馆

Suspension Culture and Somatic Embryogenesis of korean pine

Phyton-International Journal of Experimental Botany 2022年第1期91卷 223-238页

作者： Chunxue Peng Fang Gao Hao Wang Iraida Nikolaevna Tretyakova Alexander Mikhaylovich Nosov Hailong Shen Ling Yang state Key Laboratory of Tree Genetics and Breeding School of ForestryNortheast Forestry UniversityHarbin150040China state forestry and grassland administration engineering technology research center of korean pine Harbin150040China Laboratory of Forest Genetics and Breeding Institution of the Russian Academy of SciencesV.N.Sukachev Institute of Forest Siberian Branch of RASKrasnoyarsk660036Russia Department of Cell Biology and Institute of Plant Physiology K.A.Timiryazev Russian Academy of SciencesMoscow127276Russia Department of Plant Physiology Biological FacultyLomonosov Moscow State UniversityMoscow119991Russia

korean pine is an important afforestation tree species in Northeast China,which has a high ecological and economic *** regeneration of somatic embryogenesis using immature zygotic embryos of korean pine as explants has been successful,it cannot be applied to automation and large-scale ***,we urgently need a method that can increase the output of somatic embryos(SEs)to meet the needs of large-scale *** used korean pine 1-1 and 1-100 cell lines as research materials to evaluate the effects of inoculum-density,culture time,orbiting speed,vessel volume,plant growth regulator(PGR)concentration,and carbon source on the proliferation of embryogenic tissue(ET).The somatic embryogenesis ability of ET cultured in different liquid suspension media was also *** found that during liquid suspension culture of korean pine ET,the sedimented cell volume(SCV),fresh weight(FW)and dry weight(DW)were affected by inoculumdensity,culture time,orbiting speed,2,4-D concentration,6-BA concentration and carbon source *** mg⋅mL^(−1)ET were transferred to a 200 mL Erlenmeyer flask containing 20 mL liquid medium,and cultured at 100 rpm/min for 14 days to obtain the maximum *** addition,we also found that SCV,FW and DW were higher when PGRs were reduced in the liquid suspension *** substitution of maltose for sucrose resulted in slow growth of cultures and limited SE yield(13 SEs g^(−1)FW).Although culture proliferation was high at 50 rpm,SE yield was inhibited by 48%compared with 100 rpm(50 rpm=33 SEs g^(−1)FW;100 rpm/min=70 SEs g^(−1)FW).Cultivation in low-concentration PGR(1.15μM⋅L^(−1)2,4-D,0.25μM⋅L^(−1)6-BA)and sucrose liquid medium at 100 rpm/min(80 SEs g^(−1)FW)could not only promote culture proliferation but also increase SE *** determination of the suspension culture scheme of korean pine ET provides a reference for further expansion to bioreactor culture in the future and lays a foundation for the automatio

关键词： Pinus koraiensis Sieb.et Zucc embryogenic tissue liquid suspension medium cell proliferation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Quantifying soil nitrous oxide emissions in spring freezing-thawing period over different vegetation types in Northeast China

引用

Journal of Mountain Science 2022年第7期19卷 1919-1930页

作者： WU Bin MU Chang-cheng LIU Hui XU Ya-kun ZHANG Yue YANG Jia-shuo XU Wen-nian Hubei Key Laboratory of Construction and Management in Hydropower engineering China Three Gorges UniversityYichang443002China College of Hydraulic&Environmental engineering China Three Gorges UniversityYichang443002China Ministry of Education Key Laboratory of Sustainable Forest Ecosystem Management Northeast Forestry UniversityHarbin150040China Survey Planning and Design Institute of DaxinganlingNational Forestry and Grassland AdministrationDaxinganling165100China engineering research center of Eco-Environment in Three Gorges Reservoir Region Ministry of EducationChina Three Gorges UniversityYichang443002China state Key Laboratory of Forest and Soil Ecology Institute of Applied EcologyChinese Academy of SciencesShenyang110016China Hubei Key Laboratory of Disaster Prevention and Mitigation China Three Gorges UniversityYichang443002China Key Laboratory of Geological Hazards on Three Gorges Reservoir Area Ministry of EducationYichang443002China

Environmental changes significantly alter the structure,diversity and activity of soil microbial communities during spring freezing-thawing period,leading to changes in the soil microbial nitrogen *** in N_(2)O fluxes after land use conversion from primary forest to secondary forest,korean pine plantation and cropland in northeast China have not been *** experiments were conducted to measure soil N_(2)O fluxes in a primary forest,two secondary forests,a korean pine plantation,and one maize field in a temperate region in northeast China from 2017-03-06 to *** the experimental period,the soil was exclusively a nitrogen source for all land *** found that N_(2)O emissions ranged from 15.63 to 68.74μg m^(-2) h^(-1),and cumulative N_(2)O emissions ranged from 0.33 to 2.10 kg ha^(-1) during the *** N_(2)O emissions from the maize field were significantly higher than that from primary forest,korean pine plantation,hardwood forest,and Betula platyphylla forest by 262.1% to 536.4%.Compared with other ecosystems in similar studies,the N_(2)O emission rates of all ecosystem types in this study were low during the spring thaw *** multiple linear regression indicated that there were significant correlations between N_(2)O emissions and environmental factors(air temperature and soil temperature,soil water content,soil p H,NH_(4)^(+)-N,NO_(3)^(-)-N,and soil organic carbon).The results showed that conversion of land use from primary forest to hardwood forest,korean pine plantation or maize field greatly increased soil N_(2)O emissions during spring freezing-thawing period,and N_(2)O emissions from primary forest were almost the same as those from Betula platyphylla forest.

关键词： N_(2)O emission Land use change Environmental factors Nitrogen cycle

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Transcriptomic and proteomic analyses of far-red light effects in inducing shoot elongation in the presence or absence of paclobutrazol in Chinese pine

引用

Journal of forestry research 2022年第3期33卷 1033-1043页

作者： Yingtian Guo Shihui Niu Yousry A.El-Kassaby Wei Li National engineering Laboratory for Forest Tree Breeding Beijing Advanced Innovation Center for Tree Breeding by Molecular DesignCollege of Biological Sciences and TechnologyBeijing Forestry UniversityBeijing100083People’s Republic of China Department of Forest and Conservation Sciences Faculty of ForestryUniversity of British Columbia2424 Main MallVancouverBCV6T 1Z4Canada

In angiosperms, low red (R)/far-red (FR) ratio light increases gibberellin (GA) levels. GA signaling in conifer seedlings requires FR to promote shoot elongation and reduce the inhibition of shoot elongation induced by paclobutrazol (PAC), yet the effects of far-red light in inducing shoot elongation in the presence or absence of PAC is poorly understood. In this study, transcriptomic and proteomic analyses was used to examine the molecular mechanism of FR regulation of shoot elongation in Chinese pine (Pinus tabuliformis Carr.) seedlings in the presence of PAC. Four treatments were compared: white light + water (WW), FR + water (RW), white light + PAC (WP), and FR + PAC (RP), and 1436 differentially expressed genes (DEGs) and 450 differentially expressed proteins (DEPs) were identified in RW_WW (RW contrast WW), and 1862 DEGs and 481 DEPs in RP_WP (RP contrast WP). Metabolic and signal transduction pathway analyses of DEGs and DEPs in RW_WW and RP_WP, indicated that the former required more energy than the latter. Moreover, gibberellic acid, auxin, and brassinolide are equally important in RW_WW and RP_WP for shoot elongation, except for the ethylene pathway. Amino acid metabolism and cell wall organization were significantly enriched in RW_WW and RP_WP, respectively. In summary, RW_WW and RP_WP had different effects in secondary metabolism, energy metabolism, amino acid metabolism, cell wall organization, and hormone response. These results provide an important theoretical and reference basis for studying the regulatory effect of low R/FR and PAC in conifer shoot elongation.

关键词： Low R/FR ratio Pinus tabuliformis Paclobutrazol Proteome Shoot elongation Transcriptome

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：