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Effect of dietary cottonseed meal on growth performance, physiological response, and gossypol accumulation in preadult grass carp, Ctenopharyngodon idellus

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Chinese Journal of Oceanology and Limnology 2016年第5期34卷 992-1003页

作者：刘昊昆严全根韩冬金俊琰朱晓鸣杨云霞解绶启 state key laboratory of freshwater ecology and biotechnology Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China freshwater Aquaculture Collaborative Innovation Center of Hubei Province Wuhan 430070 China

Cottonseed meal(CM) was used at up to 36.95% content in the diet(replacing 60% of dietary fish meal protein) without any negative effects on growth performance of pre-adult grass carp(initial body weight,761 g) under outdoor conditions.A culture trial was conducted in net cages installed in a large concrete *** isonitrogenous and isoenergetic diets containing a gradient of CM concentrations(0,12.2%,24.4%,36.6%,48.8%,54.8%,and 61.0%) as replacement for dietary fish meal protein(0,20%,40%,60%,80%,90%,and 100%) were *** non-resistant starch(from maize) was inverse to dietary *** performance and feed utilization of fish fed the diets containing CM replacing 0-40% fishmeal protein were not affected after the 6-week feeding *** of hepatopancreatic total gossypol in the hepatopancreas was significantly correlated with free gossypol content in the diets(HTG=88.6+1.5×DFG,R^2=0.89,P<0.05).Intestinal a-amylase and γ-glutamyl transpeptidase activities rose along with increasing dietary CM *** structure of the mid-intestinal tissues and the ultrastructure of the enterocyte microvilli were normal when dietary CM was <36.6%(60% protein replacement).Increasing dietary CM content increased serum alanine aminotransferase levels but decreased serum alkaline phosphatase,cholesterol,high-density lipoprotein cholesterol,low-density lipoprotein cholesterol,triglycerides,and albumin(P<0.05).

关键词： cottonseed meal gossypol intestinal histology chymotrypsin gossypol accumulation Ctenopharyngodon idellus

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Microbial community variation in phytoremediation of triazophos by Canna indica Linn. in a hydroponic system

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Journal of Environmental Sciences 2010年第8期22卷 1225-1231页

作者： Huiping Xiao Shuiping Cheng Zhenbin Wu state key laboratory of freshwater ecology and biotechnology Institute of Hydrobiology the Chinese Academy of Sciences Wuhan 430072 China Graduate University of Chinese Academy of Sciences Beijing 100039 China

Phytoremediation of triazophos （O,O-diethyl-O-（1-phenyl-1,2,4-triazole-3-base） sulfur phosphate, TAP） pollution by Canna indica Linn. in a hydroponic system has been well studied, whereas the microbial mechanism on TAP degradation is still unknown. The variation in microbial community compositions was investigated by analyzing phospholipid fatty acids （PLFAs） profiles in microbes under TAP exposure. The TAP exposure resulted in an increase in proportions of fatty acid 16：0 and decrease in fatty acid 18：2ω9,12c, indicating that TAP may stimulate the reproduction of microorganisms and inhibit the growth of fungi to some degree. Significant correlation was found between the ratio of fungi to bacteria and TAP removal （r 2 = 0.840, p 0.01）. In addition, the microbial community in the phytoremediation system with C. indica was dominated by Gram negative bacteria, which possibly contributed to the degradation of TAP. These results indicated that TAP might induce the colonization of bacteria in the hydroponic system planted with C. indica, and lead to a discrimination of microbial community, which might be one of the mechanisms on TAP dissipation in phytoremediation system.

关键词： microbial community phospholipid fatty acids triazophos phytoremediation Canna indica Linn.

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High efficient gene targeting in rice field eel Monopterus albus by transcription activator-like effector nucleases

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Science Bulletin 2017年第3期62卷 162-164页

作者： Ke Feng Hongrui Luo Yongming Li Ji Chen Yaping Wang Yonghua Sun Zuoyan Zhu Wei Hu state key laboratory of freshwater ecology and biotechnology Institute of HydrobiologyChinese Academy of SciencesWuhan 430072China University of Chinese Academy of Science Beijing 100049China

Genome editing plays an important role in the research of gene functions,gene correction and cell replacement ***,genome editing is mainly dependent on the technology of homologous recombination(HR)and embryonic stem(ES)cell lines in mammals[1].However,genome editing is severely limited by the low efficiency of HR and lack of ES cell lines in most ***,the development of zinc finger nucleases(ZFNs),

关键词：基因打靶核酸酶转录激活胚胎干细胞 ES细胞系子样黄鳝基因组

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Submerged vegetation removal promotes shift of dominant phytoplankton functional groups in a eutrophic lake

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Journal of Environmental Sciences 2014年第8期26卷 1699-1707页

作者： Jing Dong Kai Yang Shuangshuang Li Genbao Li Lirong Song state key laboratory of freshwater ecology and biotechnology Institute of HydrobiologyChinese Academy of Sciences University of Chinese Academy of Sciences Research Center for Eco-Environmental Sciences Chinese Academy of Sciences

Historical data indicate that the dominance of submerged plants in Dianchi Lake in the 1960 s was characterized by low algal density with dominance of non-toxic group J（Scenedesmus,Pediastrum,etc.）. The removal of submerged plants,which began in the 1970 s,resulted in the expansion of bloom-forming Microcystis（group M）. laboratory experiments suggested that Microcystis aeruginosa was inclined to grow and develop at elevated temperatures. The growth of Scenedesmus obliquus was slower than that of co-cultivated M. aeruginosa in the absence of Ceratophyllum demersum,especially at higher temperatures. The existence of submerged plant C. demersum could inhibit the growth of the harmful algae M. aeruginosa and this inhibitory effect by C. demersum was enhanced with an increase in temperature. Instead,with C. demersum,the growth of S. obliquus was not inhibited,but the co-cultivated M. aeruginosa was eliminated in a short time. Combined with the historical data and laboratory experiments,it was indicated that the submerged plants might play important roles in the dominance of the non-toxic group J in the historical succession. Consequently,the introduction of the submerged plant such as C. demersum might alter the dominant phytoplankton functional groups from M to J and benefit the restoration of the eutrophic lake.

关键词： Submerged vegetation Phytoplankton Functional groups

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Gene cloning and induced expression pattern of IRF4 and IRF10 in the Asian swamp eel(Monopterus albus)

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Zoological Research 2014年第5期35卷 380-388页

作者： Qiao-Qing XU Dai-Qin YANG Rui TUO Jing WAN Ming-Xian CHANG Pin NIE School of Animal Science Yangtze University state key laboratory of freshwater ecology and biotechnology Institute of HydrobiologyChinese Academy of Sciences Hubei Collaborative Innovation Center for freshwater Aquaculture

The Asian swamp eel （Monopterus albus） is one of the most economically important freshwater fish in East Asia, but data on the immune genes of M. albus are scarce compared to other commercially important fish. A better understanding of the eel＇s immune responses may help in developing strategies for disease management, potentially improving yields and mitigating losses. In mammals, interferon regulatory factors （IRFs） play a vital role in both the innate and adaptive immune system; though among teleosts IRF4 and IRFIO have seldom been studied. In this study, we characterized IRF4 and IRFIO from M. albus （malRF4 and malRFlO） and found that malRF4 cDNA consists of 1 716 nucleotides encoding a 451 amino acid （aa） protein, while malRFlO consists of 1 744 nucleotides including an open reading frame （ORF） of 1 236 nt encoding 411 aa. The malRFlO gene was constitutively expressed at high levels in a variety of tissues, while malRF4 showed a very limited expression pattern. Expression of malRF4 and malRFlO in head kidney, and spleen tissues was significantly up-regulated from 12 h to 48 h post-stimulation with polyinosinic： polycytidylic acid （poly I：C）, lipopolysaccharide （LPS） and a common pathogenic bacteria Aeromonas hydrophila. These results suggest that IRF4 and IRF10 play roles in immune responses to both viral and bacterial infections in M. albus.

关键词： Monopterus albus IRF4 IRF10 Poly I:C Up-regulation

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Characterization of transgene integration pattern in F4 hGH-transgenic common carp (Cyprinus carpio L.)

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Cell Research 2005年第6期15卷 447-454页

作者： Bo WU1,*, Yong Hua SUN1,*, Yan Wu WANG1,2, Ya Ping WANG1, Zuo Yan ZHU1,**1 state key laboratory of freshwater ecology and biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China2 College of Life Science, Wuhan University, Wuhan 430072, China state key laboratory of freshwater ecology and biotechnology Institute of Hydrobiology Chinese Academy of Sciences Wuhan China College of Life Science Wuhan University Wuhan China

The integration pattern and adjacent host sequences of the inserted pMThGH-transgene in the F4 hGH-transgeniccommon carp were extensively studied. Here we show that each F4 transgenic fish contained about 200 copies of thepMThGH-transgene and the transgenes were integrated into the host genome generally with concatemers in a head-to-tail arrangement at 4-5 insertion sites. By using a method of plasmid rescue, four hundred copies of transgenes fromtwo individuals of F4 transgenic fish, A and B, were recovered and clarified into 6 classes. All classes of recoveredtransgenes contained either complete or partial pMThGH sequences. The class I, which comprised 83% and 84.5%respectively of the recovered transgene copies from fish A and B, had maintained the original configuration, indicatingthat most transgenes were faithfully inherited during the four generations of reproduction. The other five classes weredifferent from the original configuration in both molecular weight and restriction map, indicating that a few transgeneshad undergone mutation, rearrangement or deletion during integration and germline transmission. In the five types ofaberrant transgenes, three flanking sequences of the host genome were analyzed. These sequences were common carpβ-actin gene, common carp DNA sequences homologous to mouse phosphoglycerate kinase-1 and human epidermalkeratin 14, respectively.

关键词： transgenic common carp plasmid rescue germline transmission integration pattern.

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Biological mechanisms driving the seasonal changes in the internal loading of phosphorus in shallow lakes

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Science China Earth Sciences 2006年第Z1期49卷 14-27页

作者： XIE Ping Donghu Experimental Station of Lake Ecosystems State Key Laboratory of Freshwater Ecology and Biotechnology of ChinaInstitute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China

Because of the obvious importance of P as a nutrient that often accelerates growth of phytoplankton (including toxic cyanobacteria) and therefore worsens water quality, much interest has been devoted to P exchange across the sediment-water interface. Generally, the release mode of P from the sediment differed greatly between shallow and deep lakes, and much of the effort has been focused on iron and oxygen, and also on the relevant environmental factors, for example, turbulence and decomposition, but a large part of the P variation in shallow lakes remains unexplained. This paper reviews experimental and field studies on the mechanisms of P release from the sediment in the shallow temperate (in Europe) and subtropical (in the middle and lower reaches of the Yangtze River in China) lakes, and it is suggested that pH rather than DO might be more important in driving the seasonal dynamics of internal P loading in these shallow lakes, i.e., intense photosynthesis of phytoplankton increases pH of the lake water and thus may increase pH of the surface sediment,leading to enhanced release of P (especially iron-bound P) from the sediment. Based on the selective pump of P (but not N) from the sediment by algal blooms, it is concluded that photosynthesis which is closely related to eutrophication level is the driving force for the seasonal variation of internal P loading in shallow lakes. This is a new finding. Additionally, the selective pump of P from the sediment by algal blooms not only explains satisfactorily why both TP and PO4-P in the hypereutrophic Lake Donghu declined significantly since the mid-1980s when heavy cyanobacterial blooms were eliminated by the nontraditional biomanipulation (massive stocking of the filter-feeding silver and bighead carps), but also explains why TP in European lakes decreased remarkably in the spring clear-water phase with less phytoplankton during the seasonal succession of aquatic communities or when phytoplankton biomass was decreased

关键词： shallow lakes, internal P loading, algal blooms.

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Seasonal variation in functional phytoplankton groups in Xiangxi Bay, Three Gorges Reservoir

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Chinese Journal of Oceanology and Limnology 2011年第5期29卷 1057-1064页

作者：杨敏毕永红胡建林朱孔贤周广杰胡征宇 state key laboratory of freshwater ecology and biotechnology Institute of Hydrobiology Chinese Academy of Sciences Graduate University of Chinese Academy of Sciences Chongqing Academy of Environmental Sciences

We describe the phytoplankton dynamics and structure in Xiangxi Bay, Three Gorges Reservoir. Samples were collected monthly in the surface waters between August 2007 and July 2008. We identified 10 principle functional groups. C-strategists and S/R-strategists with a wide range of tolerance dominated the phytoplankton assemblage. Seasonal variation was related to water column stability because of changes in hydraulic operation in October, January, and May. Functional group C （Asterionellaformosa） and P （Aulacoseria granulata） dominated in August and September, whereas group Lo （Peridiniopsis niei） was the most abundant between February and April, forming a dinoflagellate bloom. Group B （Stephanodiscus hantzschii）, X2 （Komma acudata）, and Y （Cryptomonas erosa） were present throughout most of the year but were most abundant in late spring. A cyanobacterial bloom occurred from June to July, during which group M （Microcystis aeruginosa, M. wesenbergii） and H~ （Anabaenaflos-aquae） were dominant. Green algae, characterized by group G （Eudorina sp., Pandorina sp., Pyramidomonas sp.） and J （Pediastrum spp., Coelastrum spp., Scenedesums spp.）, were abundant after the bloom degraded. This sequence was corroborated by canonical correspondence analysis （CCA）. The summary sequence of functional groups resulting from CCA was： C/P→Lo→H1/M/J/G. The dynamics of the phytoplankton community may be explained by the stability of water column, irradiance, water temperature, and nutrient structure.

关键词： phytoplankton functional group Xiangxi Bay Three Gorges Reservoir

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Studies on Temporal and Spatial Variations of Phytoplankton in Lake Chaohu

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Journal of Integrative Plant Biology 2007年第4期49卷 409-418页

作者： Dao-Gui Deng Ping Xie Qiong Zhou Hua Yang Long-Gen Guo Donghu Experimental Station of Lake Ecosystems State Key Laboratory for Freshwater Ecology and Biotechnology of China Institute of Hydrobiology the Chinese Academy of Sciences Wuhan 430072 China

Temporal and spatial variations of the phytoplankton assemblage in Lake Chaohu, a large shallow eutrophic lake in China, were studied from September 2002 to August 2003. A total of 191 phytoplankton species was identified, among which Chlorophytes （101） ranked the first, followed by Cyanophytes （46） and Bacillariophytes （28）. On aver- age over the entire lake, the maximum total algal biomass appeared in June （19.70 mg/L） with a minimum （5.05 mg/ L） in November. In terms of annual mean biomass, cyanobacteria contributed 45.43% to total algal biomass, followed by Chlorophytes （27.14%）, and Bacillariophytes （20.6%）. When nitrate （NOs-N） and ammonium （NH4-N） concentrations dropped in spring, fixing-nitrogen cyanobacterium （Anabaena） developed quickly and ranked the first in terms of biomass in summer. It is likely that dominance of zooplanktivorous fish and small crustacean zooplankton favored the development of the inedible filamentous or colony forming cyanobacteria. The persistent dominance of cyanobacteria throughout all seasons may indicate a new tendency of the response of phytoplankton to eutrophication in Lake Chaohu.

关键词： cyanobacterial blooms eutrophication Lake Chaohu phytoplankton zooplankivorous fish.

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Characteristic analysis of phospholipid fatty acids(PLFAs) in typical nutrient polluted lake sediment in Wuhan

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International Journal of Sediment Research 2021年第2期36卷 221-228页

作者： Xia Zhang Qianru Chen Chuan Wang Hongpei Zhang Yuqing Zhao Liping Zhang Biyun Liu Zhenbin Wu Qiaohong Zhou College of Resources and Environmental Engineering Wuhan University of Technology state key laboratory of freshwater ecology and biotechnology Institute of Hydrobiology Chinese Academy of Sciences

The process of urbanization aggravates the endogenous pollution of urban lake sediment, and polluted sediment may seriously affect the quality of the water in lakes. At present, it is difficult to distinguish the difference between sediment that is heavily polluted by nitrogen(N) and phosphorus(P) when using an analysis based on a physicochemical index classification. The current study applied phospholipid fatty acid(PLFA) technology to further analyze the sediment characteristics from the perspective of microbiology. Surface sediment from five urban lakes that are heavily polluted with N and P in Wuhan were sampled. Statistical approaches were used to analyze the microbial community structure in the sampled sediment, and to determine the correlations between the microorganisms and physicochemical *** most severely polluted lake sediment had similar PLFA structures, the highest saturated fatty-acid content, and bacteria as the dominant microorganism. However, there were differences between the microbial biomass of the various sediment samples, which may have been related to the degree of N and P pollution. Analysis of the microbial diversity in the sediment samples indicated that the bacteria were experiencing starvation and nutrient pressure, which may have been due to the dissolved oxygen concentration of the heavily polluted lake sediment. A correlation analysis showed that the endogenous N and P had different effects on the microbes of the polluted sediment. A redundancy analysis(RDA)demonstrated that the N/P ratio had the greatest influence on the PLFA species, accounting for 83% of the cumulative interpretation. To effectively promote the role of sediment microorganisms on circulating elements, it is necessary to regulate the N/P ratio of the sediment to some extent. When the N/P ratio in sediment exceeds 6, N pollution should be prioritized.

关键词： Lake sediment Endogenous pollution Phospholipid fatty acid (PLFA) analysis Microbial population N/P ratio

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