检索结果-南通市图书馆

Complete genome sequences of four isolates of Citrus leaf blotch virus from citrus in China

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2018年第3期17卷 712-715页

作者： LI Ping LI Min ZHANG Song WANG Jun YANG Fang-yun CAO Meng-ji LI Zhong-an National Citrus Engineering research center Citrus Research Institute Southwest University College of Agronomy and Biotechnology Southwest University

Citrus leaf blotch virus （CLBV） is a member of the genus Citrivirus, in the family Betaflexiviridae. It has been reported CLBV could infect kiwi, citrus and sweet cherry in China. Of 289 citrus samples from six regions of China, 15 were detected to be infected with CLBV in this study. The complete genome of four isolates of CLBV was obtained from Reikou in Sichuan （CLBV-LH）, Yura Wase in Zhejiang （CLBV-YL）, Bingtangcheng in Hunan （CLBV-BT）, Fengjie 72-1 in Chongqing （CLBV- F J）, respectively. While they all represented 8 747 nucleotides in monopartite size, excluding the poly（A） tail, each of the isolates coded three open reading frames （ORFs）. Identity of the four isolates ranged from 98.9 to 99.8% to each other and from 96.8 to 98.1% to the citrus references in GenBank by multiple alignment of genomes. A phylogenetic tree based on the genome sequences of available CLBV isolates indicated that the four isolates were clustered together, suggesting that CLBV isolates from citrus in China did not have obvious variation. This is the first report of the complete nucleotide sequences of CLBV isolates infecting citrus in China.

关键词： Citrus leaf blotch virus (CLBV) genome sequence Citrivirus

维普期刊数据库

Multi-componential metal intercalated graphite hybrids synthesized by co-intercalation polymerization towards highefficient microwave absorptions

在线全文

学校读者我要写书评

暂无评论

Nano research 2023年第5期16卷 6369-6379页

作者： Junhua Su Qinbo Ma Longkun Que Hunan Jiang Xiaoling Xu Yong Wang Yifan Guo Zuowan Zhou Key Laboratory of Advanced Technologies of Materials(Ministry of Education) School of Materials Science and EngineeringSouthwest Jiaotong UniversityChengdu 610031China School of Chemistry Southwest Jiaotong UniversityChengdu 610031China Yibin research Institute Southwest Jiaotong UniversityYibin 644000China School of Mechanical Engineering Southwest Jiaotong UniversityChengdu 610031China research Institute of frontier Science Southwest Jiaotong UniversityChengdu 610031China

Magnetic expanded graphite(EG)hybrids were synthesized by co-intercalation polymerization of aniline together with transition metal *** results show that metal ions(Fe,Co,Ni,Cu)and even their mixtures can co-intercalate into graphite interlayers with flexibly controllable ratios and *** these co-intercalation compounds,Fe/Ni-intercalated graphite with a predesigned mole ratio of 1:3 transforms into NiFe_(2)O_(4)/FeNi_(3)@EG during the annealing *** synthesized magnetic EG hybrids present multiband microwave absorption in C and X bands due to improved impedance match as well as significantly enhanced interfacial polarization relaxation induced by multi-componential *** reflection values of−39.1 dB at 6.95 GHz and−25.7 dB at 9.4 GHz are achieved with an ultra-low loading of 5 wt.%.This work provides a flexible approach for tuning the components and structures of magnetic EG hybrids,which may contribute to the development of microwave absorption materials with superior performances.

关键词： co-intercalation intercalation polymerization expanded graphite transition metal microwave absorption

Combined effect of WS_(2) and Ti_(3)C_(2)T_(x) MXene favors oil-based ultra-low friction on rough steel-steel interface at elevated temperatures

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China(Technological Sciences) 2024年第7期67卷 1991-2002页

作者： LIU YangQin CHEN Lei WANG Yang QIAN LinMao Tribology research Institute State Key Laboratory of Rail Transit Vehicle SystemSchool of Mechanical EngineeringSouthwest Jiaotong UniversityChengdu 610031China Key Laboratory of Advanced Technologies of Materials(Ministry of Education) Research Institute of Frontier ScienceSouthwest Jiaotong UniversityChengdu 610031China

The lubrication performance of liquids is severely restricted and is degraded in high-temperature environments. Stable and reliable lubrication in high temperature environments has been a long-standing goal in various industrial fields. In this study,WS_(2)and Ti_(3)C_(2)T_(x)MXene nanoflakes were used as oil-based lubricant additives to generate ultra-low friction and even superlubricity(friction coefficient of ~0.007) at elevated temperatures(400℃), which has hitherto not been achieved by both individual pristine materials, WS_(2)and Ti_(3)C_(2)T_(x)MXene. Viscosity and thermogravimetric characterization revealed improvements in the high-temperature rheological properties and thermal stability of the lubricating base oil, indicating improved loadbearing and continuous lubrication capabilities at elevated temperatures. X-ray photoelectron spectroscopy, transmission electron microscopy, and atomic force microscopy demonstrated that the formation of an iron/titanium/tungsten-rich oxide lubricious thin film at the sliding interface reduced the interfacial shear stress, which was responsible for the observed friction and wear reductions at high contact pressures(> 1.1 GPa). Although the titanium/tungsten oxide film was gradually removed after prolonged sliding, a sufficiently thick iron oxide film maintained a low friction coefficient for at least 2 h. The improved surface quality facilitates the achievement of ultra-low friction and reduced wear. The proposed lubrication methodology has a broad utilization potential as a wear-reduction strategy across various industrial fields at elevated temperatures.

关键词： ultra-low friction high-temperature wear reduction lubricating additive tungsten disulfide MXene

维普期刊数据库

Tertiary orientation structures enhance the piezoelectricity of MXene/PVDF nanocomposite

在线全文

学校读者我要写书评

暂无评论

Nano research 2024年第6期17卷 5629-5635页

作者： Yong Ao Tao Yang Guo Tian Shenglong Wang Tianpei Xu Lin Deng Jieling Zhang Lihua Tang Weili Deng Long Jin Weiqing Yang Key Laboratory of Advanced Technologies of Materials(Ministry of Education) School of Materials Science and EngineeringSouthwest Jiaotong UniversityChengdu 610031China Department of Mechanical Engineering The University of AucklandAuckland 1010New Zealand research Institute of frontier Science Southwest Jiaotong UniversityChengdu 610031China

With the increasing demand for flexible piezoelectric sensor components,research on polyvinylidene fluoride(PVDF)based piezoelectric polymers is mounting ***,the low dipole polarization and disordered polarization direction presented in PVDF hinder further improvement of piezoelectric ***,we constructed an oriented tertiary structure,consisting of molecular chains,crystalline region,and MXene sheets,in MXene/PVDF nanocomposite via a temperature-pressure dual-field regulation *** highly oriented PVDF molecular chains form approximately 90%of theβ*** addition,the crystalline region structure with long-range orientation achieves out of plane polarization *** parallel orientation arrangement of MXene effectively enhances the piezoelectric performances of the nanocomposite,and the current output of the device increases by nearly 23 *** high output device is used to monitor exercise action,exploring the potential applications in wearable electronics.

关键词： flexible sensor piezoelectric MXene/polyvinylidene fluoride(PVDF)nanocomposite orientation structures

Programmable activation of Bombyx gene expression using CRISPR/dCas9 fusion systems

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Insect Science 2019年第6期26卷 983-990页

作者： Xiao-Gang Wang San-Yuan Ma Jia-Song Chang Run Shi Ruo-Lin Wang Ping Zhao Qing-You Xia State Key Laboratory of Silkworm Genome Biology Southwest University ChongqingChina Chongqing Engineering and Technology research center for Novel Silk Materials Southwest University Chongqing China

The recently developed clustered regularly interspaced short palindromic repeats (CRISPR)-based techniques have made it possible to reprogram target gene expression without cloning complementary DNA or disturbing genomic sequence in mammalian cells and several multicellular organisms. We previously showed that CRISPR-associated protein 9 (Cas9) and CRISPR from Prevotella and Francisella 1 (Cpfl) could induce target mutations, deletions, inversions, and duplications both singly and multiplex in silkworm, Bombyx mori. However, it remains unknown whether the CRISPR activation (CRISPRa) system can be used in B. mori. In this study, we investigated the CRISPRa system, in which a nuclease dead Streptococcus pyogenes Cas9 (SpCas9) is fused to two transcription activation domains, including VP64 (a tetramer of the herpes simplex VP 16 transcriptional activator domain), and VPR (a tripartite activator, composed of VP64, p65, and Rta). The results showed that both dCas9-VP64 and dCas9-VPR systems could be used in B. mori cells, of which the latter showed significantly higher activity. The dCas9-VPR system showed considerable activity on all five tested target genes, and further analysis revealed that the up-regulation of genes was negatively correlated to their basal expression level. We also observed that this system could be used to upregulate a range of target genes. Taken together, our findings demonstrate that CRISPRa can be a powerful tool to study gene functions in B. mori and perhaps other non-drosophila insects.

关键词： Bombyx mori CRISPR/dCas9 system transcriptional activation VP64 VPR

A secreted phospholipase A_(2) (BmsPLA_(2)) regulates melanization of immunity through BmDDC in the silkworm Bombyx mori

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Insect Science 2023年第6期30卷 1579-1594页

作者： Tian Li Gemin Wang Wei He Guiqin Li Chunyang Wang Jiamei Zhao Pan Chen Meiwei Guo Ping Chen College of Sericulture Textile and Biomass SciencesSouthwest UniversityChongqingChina Drug Discovery research center Southwest Medical UniversityLuzhouChina State Key Laboratory of Silkworm Genome Biology Southwest UniversityChongqingChina

Insect immune-associated phospholipase A_(2) (PLA_(2)) is an important target of pathogen invasion. Melanization, an effective defense response, has significant correlations with other immune responses to coordinate immune attack against invaders. However, the effect of PLA_(2) on melanization has not yet been reported in insects or other arthropods. In this work, we cloned a PLA_(2) gene (BmsPLA_(2)), and its protein had characteristic features of secreted PLA_(2) (sPLA_(2)). After injection of bacteria, BmsPLA_(2) expression and sPLA_(2) activity in hemolymph significantly increased. BmsPLA_(2) fluorescence was transferred from the cytoplasm to the cell membranes of circulating hemocytes. These results indicated that BmsPLA_(2) was related to hemolymph immunity in silkworms. Interestingly, reducing BmsPLA_(2) by RNA interference decreased melanosis (melanistic hemocytes) levels in vivo and in vitro, while BmsPLA_(2) overexpression had the opposite effect. The larval survival and melanization rate in the hemocoel both slowed depending on the PLA_(2) inhibitor dosage. These results demonstrated that BmsPLA_(2) plays a role in melanization during the immune process of silkworms. Surprisingly, the level of BmDDC matched the degree of melanization in various observations. BmDDC expression showed a significant increase, with the peak occurring later than that of BmsPLA_(2) after injection of bacteria, implying that BmsPLA_(2) was activated prior to BmDDC. Moreover, the alteration of BmsPLA_(2) by RNA interference or overexpression led to altered BmDDC levels. These results suggested that BmsPLA_(2) regulates the melanization response in silkworms through BmDDC. Our study proposes a new regulatory mechanism of the melanization response and new directions for understanding the complex immune networks of insects.

关键词： DOPA decarboxylase immunity melanization phospholipase A_(2) silkworm

Gradient CNT/PVDF piezoelectric composite with enhanced force-electric coupling for soccer training

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nano research 2023年第8期16卷 11312-11319页

作者： Wanghong Zeng Weili Deng Tao Yang Shenglong Wang Yue Sun Jieling Zhang Xiarong Ren Long Jin Lihua Tang Weiqing Yang Key Laboratory of Advanced Technologies of Materials(Ministry of Education) School of Materials Science and EngineeringSouthwest Jiaotong UniversityChengdu 610031China Department of Mechanical and Mechatronics Engineering University of AucklandAuckland 1010New Zealand research Institute of frontier Science Southwest Jiaotong UniversityChengdu 610031China

Real-time monitoring of ball–shoe interactions can provide essential information for high-quality instruction in personalized soccer training,yet existing monitoring systems struggle to reflect specific forces,loci,and durations of ***,we design a self-powered piezoelectric sensor constructed by the gradient carbon nanotube/polyvinylidene fluoride(CNT/PVDF)composite to monitor the interactions between the ball and the ***-dimensional Raman mapping demonstrates the gradient structure of CNT/PVDF prepared by programmable electrospinning combined with a hot *** from the synergistic effect of local polarization caused by the enrichment of CNT and the reduced diffusion of silver patterns in gradient structure,the as-prepared composite exhibits enhanced force-electric coupling with an excellent sensitivity of 80 mV/N and durability over 15,000 *** this basis,we conformally attach a 3×3 sensor array to a soccer shoe,enabling real-time acquisition of kick position and contact force,which could provide quantitative assessment and personalize guidance for the training of soccer *** self-powered piezoelectric sensor network system offers a promising paradigm for wearable monitoring under strong impact forces.

关键词： mechanical and electrical performance pressure sensor carbon nanotube/polyvinylidene fluoride(CNT/PVDF)composite soccer training

Progress in graphene-based magnetic hybrids towards highly efficiency for microwave absorption

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Materials Science & Technology 2022年第11期106卷 147-161页

作者： Fuxi Peng Mingfeng Dai Zhenyu Wang Yifan Guo Zuowan Zhou Key Laboratory of Advanced Technologies of Materials(Ministry of Education) School of Materials Science and EngineeringSouthwest Jiaotong UniversityChengdu 610031China Institute of frontier Science and Technology Southwest Jiaotong UniversityChengdu 610031China

Nowadays, the yearning for microwave absorption materials(MAMs) are more and more urgent for dealing with the increasingly serious electromagnetic pollution and the demand of modern military *** potential candidates, the graphene(GE) based magnetic hybrids have advantages in structural controllable and designing flexibility, providing opportunities for achieving highly efficiency of microwave absorption(MA). Thus, the structural regulation and MA performances of GE-based magnetic hybrids arouse great attention in related fields. In this review, we summarize the recently progress in MA performance of GE-based magnetic hybrids. Typical absorption process and corresponding mechanism are firstly introduced, for guiding the design of GE-based magnetic MAMs. Then, the magnetic components, synthesis methods, structural features and regulation strategies of these GE-related magnetic materials are reviewed, and their influences on MA performances have also been discussed. Challenges, and prospects of the GE-based magnetic MAMs are suggested. This review provides a brief but systematic introduction to GE-based magnetic MAMs, which may pave the way for the design of MAMs with highly efficient MA performances.

关键词： Graphene-based magnetic hybrids Microwave absorption Structural regulation Impedance matching Dissipative capacity

维普期刊数据库

CRISPR/dCas9-mediated imaging of endogenous genomic loci in living Bombyx mori cells

在线全文

学校读者我要写书评

暂无评论

Insect Science 2020年第6期27卷 1360-1364页

作者： Wei-Qing Xing San-Yuan Ma Yuan-Yuan Liu Qing-You Xia Biological Science research center Southwest UniversityChongqingChina Chongqing Engineering and Technology research center for Novel Silk Materials Southwest UniversityChongqingChina Chongqing Key Laboratory of Sericulture Southwest UniversityChongqingChina Department of Hematology Southwest HospitalThird Military Medical University(Army Medical University)ChongqingChina

Dear Editor,Visualization of specific endogenous genomic loci is crucial to understand the molecular basis underlying the spatiotemporal regulation of gene *** genetic loci were previously visualized primarily via flu... 详细信息

关键词： endogenous expression. al.