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用新的紫外可见UV—160型分光光度计改善定量分析

第五届亚太地区新生儿筛查学术交流会

作者： Shuichi Kawana Katsuhiro Nakagawa Takashi Yamamoto Yuki Hashi Qin Ya Ping Zhou Hai Xia Masahiko Kimura Seiji Yamaguchi shimadzu corporation No.1 Nishinokyo-Kuwabaracho Nakagyo-ku Kyoto 604-8511 Japan shimadzu corporation No.1 Nishinokyo-Kuwabaracho Nakagyo-ku Kyoto 604-8511 Japan shimadzu SYSTEM DEVELOPMENT corporation 2-11 Sanjyo Boh-Cho Nishinokyo Nakagyo- ku Kyoto 604-8435 JAPAN shimadzu （HONGKONG） LIMITED 14F No.16Chao Yang Men Wai Street Beijing China shimadzu （HONGKONG） LIMITED 14F No.16Chao Yang Men Wai Street Beijing China shimadzu （HONGKONG） LIMITED 14F No.16Chao Yang Men Wai Street Beijing China Shimane University Faculty of Medicine 89-1 En-ya-cho Izumo Shimane 693-8501 JAPAN Shimane University Faculty of Medicine 89-1 En-ya-cho Izumo Shimane 693-8501 JAPAN

＜正＞The tandem MS is usually used for the screening of inherited disorders because of shorter analysis time. To obtain more accurate quantity information in close inspection and evaluation of curative effect the gas chromatography - mass spectrometer （GC/MS） method is used. It usually takes one hour to analyse the extract that is treated from the urine sample. The reduction of analysis time has been required to improve the analytical productivity. Fast GC/MS can dramatically reduce the analysis run time without sacrificing the analytical performance. Narrow bore （＜0.1 mm I.D.） capillary columns can shorten the analysis time without loss in the separation efficiency.

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光谱学与光谱分析 1985年第6期 63-64页

作者： Tatsumi Sato,Toshiaki Fukuma,0samu Ando,张桂兰 Analytical Instrument Division shimadzu corporation I Nishinokyo-Kuwabaracho Nakagyo-Ku Kyoto 604 Japan Analytical Instrument Division shimadzu corporation I Nishinokyo-Kuwabaracho Nakagyo-Ku Kyoto 604 Japan Analytical Instrument Division shimadzu corporation I Nishinokyo-Kuwabaracho Nakagyo-Ku Kyoto 604 Japan

由于用做定量测量的分光光度计的设计主要是用于常规分析,因此最重要的是要能容易和快速的测量样品。普通的分光光度计不能满足这些要求的,因为它没有快速的波长定位速度和足够高的定量功能。新发展的紫外可见UV-160型分光光度计,适用... 详细信息

由于用做定量测量的分光光度计的设计主要是用于常规分析,因此最重要的是要能容易和快速的测量样品。普通的分光光度计不能满足这些要求的,因为它没有快速的波长定位速度和足够高的定量功能。新发展的紫外可见UV-160型分光光度计,适用于这些测量。它是由一个单色仪,一个键盘,一个图示阴极射线管和一个绘图仪组成的。波长定位速度高达60nm/秒。测量吸光度光谱时,扫描速度可达40nm/秒。这些速度比普通分光光度计要快三倍多,而且能迅速地作多波长测量。

关键词：分光光度计光学式分析仪器紫外可见 UV 定量分析

A multiplex method for detection of SARS-CoV-2 variants based on MALDI-TOF mass spectrometry

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Biosafety and Health 2023年第2期5卷 101-107页

作者： Ziyuan Zhao Liying Sun Liqin Wang Xiaodong Li Junping Peng NHC Key Laboratory of Systems Biology of Pathogens Institute of Pathogen BiologyChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100730China Key Laboratory of Respiratory Disease Pathogenomics Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China shimadzu China Innovation Center Shimadzu CorporationBeijing 100020China

The recent outbreak of the coronavirus disease 2019(COVID-19)pandemic and the continuous evolution of the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)have highlighted the significance of new detection methods for global monitoring and *** quantitative reverse transcription PCR(RT-qPCR),the current gold standard for diagnosis,performs excellently in genetic testing,its multiplexing capability is limited because of the signal crosstalk of various ***,we present a highly effi-cient platform which combines 17-plex assays with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF MS),enabling the targeting of 14 different mutation sites of the spike *** using a set of 324 nasopharyngeal swabs or sputum clinical samples with SARS-CoV-2 MS method was identical to that with the *** detection consistency of mutation sites was 97.9%(47/48)com-pared to Sanger sequencing without cross-reaction with other respiratory-related ***,the MS method is highly potent to track and assess SARS-CoV-2 changes in a timely manner,thereby aiding the continuous response to viral variation and prevention of further transmission.

关键词： COVID-19 SARS-CoV-2 Multiplexing capability MALDI-TOF mass spectrometry Viral variation

Improvement of the Assay Method for Steviol Glycosides in the JECFA Specifications

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American Journal of Analytical Chemistry 2013年第4期4卷 190-196页

作者： Atsuko Tada Kyoko Ishizuki Junichi Iwamura Hirohisa Mikami Yoshiko Hirao Isao Fujita Takeshi Yamazaki Hiroshi Akiyama Yoko Kawamura Laboratory of Creative Science Co. Ltd. Misonocho Japan Morita Kagaku Kogyo Co. Ltd. Osaka Japan National Institute of Health Sciences Tokyo Japan shimadzu corporation Kyoto Japan

Steviol glycosides are natural sweetener constituents found in the leaves of Stevia rebaudiana Bertoni (Asteraceae). The specifications for steviol glycosides were established by the Joint FAO/WHO Expert Committee on Food Additives (JECFA) in 2008, although there was a call in the following year for the modification of this assay method to enable the determination of nine steviol glycosides rather than just seven. In response, based on a proposed method by the Japan Stevia Association, we developed an improved method by changing the HPLC conditions and including the use of an octadecylsilyl column instead of an amino-bonded column to enable the rapid and reliable determination of the nine steviol glycosides by an isocratic HPLC-UV method. With the developed method, the nine steviol glycosides can be separately determined, and identified using individual reference chemicals as standards, unlike the previous identification method, which was based on the relative retention times. In addition, the single stevioside quantification standard was replaced with both stevioside and rebaudioside A quantification standards. Importantly, the validation of the developed method was successful. The limits of quantification for the nine steviol glycosides were between 0.2% and 0.6%. The developed assay method for the nine steviol glycosides was proposed to JECFA and adopted as the revised assay method for the steviol glycosides specifications at its 73rd meeting in 2010.

关键词： Steviol Glycosides Stevioside Rebaudioside A Reversed-Phase HPLC JECFA Specifications

Differences of Plasma Levels of Tryptophan, Serotonin, 5-Hydroxyindole Acetic Acid, and Kynurenine between Healthy People and Patients of Major Monopolar Depression at Various Age and Gender

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Food and Nutrition Sciences 2020年第6期11卷 431-441页

作者： Hiroi Tomioka Junichi Masuda Akikazu Takada Akira Iwanami Department of Psychiatry School of Medicine Showa University Tokyo Japan Department of Psychiatry Showa University Northern Yokohama Hospital Yokohama Japan Global Application Development Center Shimadzu Corporation Tokyo Japan International Projects on Food and Health (NPO) Tokyo Japan

Background: It is not well analyzed whether there are differences in plasma levels of tryptophan (TRP) metabolites between healthy control people (HC) and patients of major monopolar depression (MMD). Methods: Ultra high-speed liquid chromatography/mass spectrometry has been used for the simultaneous determination of plasma levels of tryptophan metabolites in depressive patients. Results: There are no significant differences between plasma levels of TRP between HC and MMD. Plasma levels of TRP of HC are higher in young men, young women, old men, and old women in this order. Serotonin (5-HT) levels are higher in MMD than HC. Plasma levels of 5-HIAA of HC are also higher than those of patients of MMD. Plasma levels of kynurenine (KYN) of healthy old men and old women are higher than those of young men and old women. Plasma levels of KYN are higher in old women and young men of MMD than those of HC. Conclusion: Plasma levels of 5-HT are higher in patients of MMD than those of HC, which may suggest that use of drugs inhibiting the 5-HT transportation may increase plasma levels of 5-HT in MMD.

关键词： Depression Monopolar Depression Bipolar Depression Tryptophan Serotonin 5-Hydroxyindole Acetic Acid Kynurenine 3-Hydroxykynurenine Kynurenic Acid Anthranilic Acid Xanthurenic Acid Indole-3-Acetic Acid SSRI (Selective Serotonin Reuptake Inhibitor) SNRI (Serotonin Norepinephrine Reuptake Inhibitor)

Comparison of Plasma Levels of Tryptophan Metabolites between Healthy People and Patients of Bipolar Depression at Various Age and Gender

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Journal of Biomedical Science and Engineering 2020年第6期13卷 120-129页

作者： Hiroi Tomioka Junichi Masuda Akikazu Takada Akira Iwanami Department of Psychiatry Showa University School of Medicine Tokyo Japan Department of Psychiatry Showa University Northern Yokohama Hospital Yokohama Japan Global Application Development Center Shimadzu Corporation Kanagawa-ken Japan International Projects on Food and Health (NPO) Tokyo Japan

Background: It is not well analyzed whether there are differences in plasma levels of tryptophan (TRP) metabolites between healthy control people (HC) and patients of type II bipolar depression (BDII). Methods: Ultra high-speed liquid chromatography/mass spectrometry has been used for the simultaneous determination of plasma levels of tryptophan metabolites in depressive patients. Results: Plasma levels of TRP are not different between HC and patients of BDII. Serotonin (5-HT) levels are higher in BDII than HC. Plasma levels of 5-HIAA of HC are higher than those of old women of BDII, but lower in young women of BDII. Plasma levels of kynurenine (KYN) of HC are not different from those of patients of BDII. Conclusion: Plasma levels of 5-HT are higher in patients of BDII than those of HC, which may suggest that use of drugs inhibiting the 5-HT transportation and lower transporter biding may increase plasma levels of 5-HT in patients of BD.

关键词： Depression Monopolar Depression Bipolar Depression Tryptophan Serotonin 5-Hydroxyindole Acetic Acid Kynurenine SSRI (Selective Serotonin Reuptake Inhibitor) SNRI (Serotonin Norepinephrine Reuptake Inhibitor) Anxiolytic Antipsychotic

Comparison of Age and Gender Differences of Tryptophan Metabolites in Patients of Major Monopolar and Bipolar Depression

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Food and Nutrition Sciences 2020年第3期11卷 172-185页

作者： Hiroi Tomioka Junichi Masuda Akikazu Takada Akira Iwanami Department of Psychiatry Showa University School of Medicine Showa Japan Department of Psychiatry Showa University Northern Yokohama Hospital Showa Japan Global Application Development Center Shimadzu Corporation Kanagawa-ken Japan International Projects on Food and Health (NPO) Tokyo Japan

Background: No research has been done for the determination of plasma levels of tryptophan metabolites in patients of monopolar and bipolar depression. Methods: Ultra high-speed liquid chromatography/mass spectrometry has been used for the simultaneous determination of plasma levels of tryptophan metabolites in depressive patients. Results: No significant age and gender differences were shown in monopolar depressive patients and some differences were shown in bipolar patients. The administration of drugs such as antidepressants, antipsychotics, mood stabilizers do not seem to have affected the results. Conclusion: In patients of major monopolar depression age and gender differences of plasma levels of tryptophan metabolites disappear although significant differences are observed in healthy volunteers. Some differences of age and gender differences were shown between monopolar and bipolar depressive patients.

Co-Sense for BA LC/MS系统对血浆中药物的直接分析

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Co-Sense for BA LC/MS系统对血浆中药物的直接分析

Effects of Gender, Age, and Clot Formation on the Measurements of Tryptophan Metabolites in Blood

第十五次全国色谱学术报告会

作者：宋苑苑 Hashi Yuki 吕冬 Masatoshi Takahashi Shin-ichi Kawano Keiko Yamabe Eiichi Yamamoto Naoki Asakawa 岛津国际贸易(上海)有限公司北京分析中心岛津国际贸易(上海)有限公司北京分析中心岛津国际贸易(上海)有限公司北京分析中心 shimadzu corporation Japan shimadzu corporation Japan shimadzu corporation Japan Eisai Co．Ltd． Japan Eisai Co．Ltd． Japan

＜正＞本文介绍采用Co-Sense for BA LC/MS系统对血浆中药物进行直接分析。该系统运用柱切换技术、含有稀释旁路与前处理柱的自动在线前处理系统以及MS检测器（电喷雾接口）。在药代动力学及代谢研究中需要对血清、血浆等生物样品中的... 详细信息

＜正＞本文介绍采用Co-Sense for BA LC/MS系统对血浆中药物进行直接分析。该系统运用柱切换技术、含有稀释旁路与前处理柱的自动在线前处理系统以及MS检测器（电喷雾接口）。在药代动力学及代谢研究中需要对血清、血浆等生物样品中的药物进行分析。为防止柱子的堵塞、劣化,在保护LC/MS接口的同时提高药物的回收率,分析前需要对生物样品进行除蛋白的处理。自动在线前处理系统Co-Sense for BA使用反相前处理柱（Shim-pack MAYI-ODS）和在线稀释旁路,可实现对血清、血浆等生物样品的直接进样分析。

关键词： Co-Sense-LC/MS 在线前处理系统药物分析

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Food and Nutrition Sciences 2019年第7期10卷 761-775页

作者： Junichi Masuda Masato Karayama Takafumi Suda Masato Maekawa Fumiko Shimizu Akikazu Takada Global Application Development Center Shimadzu Corporation Kanagawa-ken Japan Second Department of Internal Medicine Hamamatsu University School of Medicine Hamamatsu Japan Department of Laboratory Medicine Hamamatsu University School of Medicine Hamamatsu Japan Faculty of Life and Environmental Sciences Showa Women’s University Tokyo Japan International Projects on Food and Health (NPO) Tokyo Japan

Background: Tryptophan metabolism plays important roles in health and diseases. Although simultaneous measurements of tryptophan metabolites are successfully measured, influences of age, gender, and clot formation on the measurements have not been reported. Methods: We took blood from young and old Japanese men and women and compared plasma levels of tryptophan metabolites. We also took plasma and serum from the blood of middle-aged men (n = 10). Metabolites analysis was performed by a liquid chromatograph tandem mass spectrometer, the LCMS-8060 quadrupole mass spectrometer combined with Nexera X2 liquid chromatograph system (shimadzu corporation, Kyoto, Japan). Body mass index (BMI) and TRP metabolites have been measured in healthy young men (n = 48), young women (n = 47), old men (n = 44), and old women (n = 39). TRP metabolites were measured by using the ultrahigh speed liquid chromatography-mass spectroscopy (shimadzu corporation). Results: Tryptophan and its metabolites such as serotonin, 5-hydroxyindole acetic acid, indole-3-acetic acid, kynurenine, anthranilic acid, were higher in young women and old men than young men and old women. Plasma levels of 3-hydroxykynurenine and xanthurenic acid were lower in young women and old men. Comparison of plasma and serum indicates that most of metabolites were higher in serum than plasma except for 3-hydroxy-kynurenine and quinolinic acid. Conclusion: Metabolites of the upper stream of degradation of tryptophan were higher in young women and old men than young men and old women, which suggests that the degradation of tryptophan was accelerated in young men and old women than young women and old men. Serum preparation may activate tryptophan degradation resulting in higher levels of metabolites in serum than in plasma.

关键词： Plasma Serum Platelet Tryptophan Serotonin 5-Hydroxyindole Acetic Acid Kynurenine 3-Hydroxykynurenine Kynurenic Acid Anthranilic Acid Xanthurenic Acid Indole-3-Acetic Acid

Evaluating “Cosmetic Therapy” by Using Near-Infrared Spectroscopy

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World Journal of Neuroscience 2014年第2期4卷 194-201页

作者： Mayumi Ikeuchi Keishi Saruwatari Yumi Takada Mika Shimoda Ayako Nakashima Masao Inoue Takashige Oroguchi Naoaki Ishii Fumihito Yoshii Munetaka Haida Department of Neurology Tokai University School of Medicine Kanagawa Japan Department of Nursing Tokai University School of Health Sciences Kanagawa Japan Kanebo Cosmetics Inc. Beauty Research Laboratory Tokyo Japan Kanebo Cosmetics Inc. Innovative Beauty Science Laboratory Kanagawa Japan Life Care Center Tokai University School of Medicine Kanagawa Japan shimadzu corporation Market Development Group Tokyo Japan Tokai University Junior College of Nursing and Medical Technology Kanagawa Japan

This study examined the effect of cosmetic therapy on frontal lobe activation as revealed by topographic near-infrared spectroscopy (NIRS). We evaluated emotional responses to a photograph of a face with/without makeup by 22 healthy female volunteers (mean age, 52 ± 10.5 years). The results of the first-round analysis showed a significant increase of oxy-Hb in the frontal lobe area when the subject looked at a photograph of herself made up as compared to not made up. In a later round of analysis, we divided the subjects into 2 groups having contrasting scores on the Profile of Mood States-Short Form Japanese version. One group was classed as “high vigor” (a common standard pattern) and the other as “low vigor” (depression-tendency pattern). The made-up/not made-up difference did not have any effect on the oxy-Hb level in the frontal lobe in the high vigor group. In contrast, makeup produced a significant increase in the oxy-Hb level over a wide frontal area in the low vigor group, which indicated widespread frontal lobe activation. This result indicates a beneficial effect of cosmetic therapy on the brain function of patients with depression and/ or dementia.

关键词： NIRS Frontal Lobe Cosmetic Therapy Depression Emotion POMS