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Effect of miR-124 Overexpression under Wnt/β-catenin signaling Pathway on Inflammatory Factor and Epidermal Growth Factor in Rats with Precancerous Gastric Lesions

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Chinese Journal of Biomedical Engineering(English Edition) 2024年第1期33卷 17-26页

作者： ZHOU Li-jiang Zhejiang Chinese Medical University Hangzhou Zhejiang 310000China shangyu people's hospital of shaoxing Shaoxing Zhejiang 312300China

Objective:To investigate the effects of miR-124 overexpression on inflammatory factors and epidermal growth factor in rats with gastric precancerous lesions by modulating the Wnt/β-catenin signaling ***:Eighty sPF-grade Wistar rats were selected as the research *** adaptive feeding,they were divided into 4 groups using a random number table *** control group was fed a regular diet,while the model group,low expression group,and overexpression group of rats were treated with N-methyl-N’-nitro-N-nitrosoguanidine to establish a model of gastric cancer precancerous *** successful modeling,the control group and model group rats were injected intraperitoneally with 0.9%sodium chloride solution,the low-expression group rats were injected intraperitoneally with miR-124-5p inhibitor,and the over-expression group rats were injected intraperitoneally with miR-124-5p *** 8 weeks of continuous injection,the levels of miR-124,Wnt/β-catenin signaling pathway-related proteins,inflammatory factors,and epidermal growth factor were observed and compared among the four groups of ***:The differences in the relative expression of miR-124 among the four groups of rats were statistically significant(Ps in the expression of Wnt protein andβ-catenin protein among the four groups of rats were statistically significant(Pssion of Wnt protein andβ-catenin protein in the overexpression group was significantly higher than that of the control group,and significantly lower than that in the model group and the low-expression group;the levels of IL-1β,IL-6,and TNF-αof rats in the four groups of rats IL-1β,IL-6 and TNF-αlevels were statistically different(Ps of IL-1β,IL-6 and TNF-αin rats in the overexpression group were higher than those in the model group and significantly lower than those in the model group and the low-expression group(Ps in the levels of EGF,HER1

关键词： precancerous lesions of the stomach Wnt/β-catenin signal pathway miR-124 inflammatory factor epidermal growth factor

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Reoperation for heterochronic intraductal papillary mucinous neoplasm of the pancreas after bile duct neoplasm resection:A case report

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World Journal of Gastrointestinal surgery 2023年第7期15卷 1542-1548页

作者： Gang Xiao Tao Xia Yi-Ping Mou Yu-Cheng Zhou Department of Hepatopancreatobiliary surgery Shaoxing People’s HospitalShaoxing 312000Zhejiang ProvinceChina Division of Gastrointestinal and Pancreatic surgery Department of General SurgeryZhejiang Provincial People’s Hospital(People’s Hospital of Hangzhou Medical College)Key Laboratory of Gastroenterology of Zhejiang ProvinceHangzhou 310014Zhejiang ProvinceChina

BACKGROUND Intraductal papillary neoplasm of the bile duct(IPNB)and intraductal papillary mucinous neoplasm(IPMN)of the pancreas have similar pathological ***,they often develop separately and it is rare for both to occur *** presenting with heterochronic IPMN after IPNB are prone to be misdiagnosed with tumor *** sUMMARY A 67-year-old male patient was admitted 8.5 years after IPNB carcinoma and 4 years after the discovery of a pancreatic tumor.A left hepatic bile duct tumor with distal bile duct dilatation was found 8.5 years ago by the computed tomography;therefore,a left hepatectomy was *** postoperative pathological diagnosis was malignant IPNB with negative cutting edge and pathological stage *** resonance imaging 4 years ago showed cystic lesions in the pancreatic head with pancreatic duct dilatation,and carcinoembryonic antigen continued to *** emission tomography showed a maximum standard uptake value of 11.8 in the soft tissue mass in the pancreatic head,and a malignant tumor was *** pancreatoduodenectomy was *** pathological diagnosis was pancreatic head IPMN with negative cutting edge,pancreaticobiliary type,stage *** was discharged 15 d after the ***-up for 6 mo showed no tumor recurrence,and quality of life was *** IPNB and IPMN are precancerous lesions with similar pathological characteristics and require active surgery and long-term follow-up.

关键词： Intraductal papillary neoplasm of the bile duct Intraductal papillary mucinous neoplasm of the pancreas Pancreatoduodenectomy Heterochronous tumor Reoperation Case report

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serum Levels of RBP4 Might Not Be Determined by Diabetes Mellitus but by Kidney Function and Renal Replacement Therapy

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Journal of shanghai Jiaotong university(science) 2016年第6期21卷 757-762页

作者：芦泽源张光远谢玉婷李军辉汪年松晏春根王锋 Department of Nephrology Shanghai Eighth People's Hospital Department of Nephrology Shanghai Jiaotong University Affiliated Sixth People's Hospital Department of Urology Zhongda Hospital of Southeast University Department of Internal Medicine Affiliated Hospital of Shaoxing University

It has been reported that retinol-binding protein 4(RBP4) is associated to adiposity,insulin resistance,and type 2 ***,circulating RBP4 levels are also affected by renal *** aim of the present study is to investigate whether serum levels of RBP4 are primarily associated with different stages of chronic kidney disease(CKD) or type 2 diabetes,if there is more potential relevance between RBP4 and renal replacement *** serum levels of RBP4 were assessed by commercial competitive enzyme-linked immunosorbent assay(ELIsA)kit in 212 patients with the CKD stages 1—5 and in 24 healthy controls,while its correlation with clinical and metabolic parameters was *** serum level of RBP4 had a strong correlation with estimated glomerular filtration rate(e GFR)(P stratified by e GFR and treatment,no more differences in RBP4 serum concentration were detected between type 2 diabetic and non-diabetic subjects [CKD stages 1—5,non-dialysis(ND),hemodialysis(HD) and peritoneal dialysis(PD);P > 0.05 for all].The elevation of RBP4 become higher in HD than in PD and ND in CKD5 patients(P = 0.008 and P = 0.04,respectively),while there was no significant difference between PD and ND *** linear regression analysis demonstrated three independent predictors of e GFR(β =-0.676,P study *** study results demonstrated that the serum level of RBP4 was negatively related to the e GFR,whether diabetes mellitus(DM) affected the blood concentration of RBP4 or *** the serum level of RBP4 exhibited significant difference in different renal replacement therapies.

关键词： chronic kidney disease(CKD) retinol binding protein 4(RBP4) renal replacement therapy diabetes mellitus(DM)

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Effect of exercise during pregnancy on offspring development through ameliorating high glucose and hypoxia in gestational diabetes mellitus

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World Journal of Diabetes 2024年第11期15卷 2203-2219页

作者： Yi-Bo Tang Le-sha Wang Yi-Hui Wu Li-Xia Zhang Lu-Yao Hu Qi Wu Meng-Lin Zhou Zhao-Xia Liang Department of Obstetrics Women's Hospital School of MedicineZhejiang UniversityHangzhou 310006Zhejiang ProvinceChina Department of Obstetrics Shaoxing People’s HospitalShaoxing 312000Zhejiang ProvinceChina

BACKGROUND Gestational diabetes mellitus(GDM)women require prenatal care to minimize short-and long-term *** mechanism by which exercise during pregnancy affects organ development and whether glucose transporter(GLUT)1 plays a role in GDM offspring organ development remains *** To determine the effect of exercise during pregnancy on the cardiac,hepatic and renal development of GDM mother’s *** Placenta samples were collected from humans and *** mouse models were created using streptozotocin along with a GDM with exercise *** hearts,livers and kidneys of 3-and 8-week-old offspring were collected for body composition analysis and *** effects of high glucose levels and hypoxia were investigated using HTR8/*** and wound-healing assays were performed to assess cell *** accompanied with TUNEL and Ki67 staining was used to explore apoptosis and *** Exercise during pregnancy downregulated the GLUT1 and hypoxia inducible factor-1αexpression in placenta from individuals with *** chloride induced hypoxia and high glucose levels also significantly decreased migration and apoptosis of HTR8/sVneo *** addition,exercise reduced inflammatory cell infiltration in the liver and decreased the tubular vacuolar area in the kidneys of *** GDM affects the growth and development of organs in *** during pregnancy can reverse adverse effects of GDM on the development of the heart,liver,and kidney in offspring.

关键词： Gestational diabetes mellitus Exercise Glucose transporter 1 Hypoxia inducible factor-1α Placenta Offspring

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Effects of WeChat platform-based health management on health and self-management effectiveness of patients with severe chronic heart failure

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World Journal of Clinical Cases 2021年第34期9卷 10576-10584页

作者： Zhan-Ru Wang Jia-Wu Zhou Xiao-Ping Liu Guo-Juan Cai Qi-Hong Zhang Jun-Fang Mao Department of Critical Care Medicine Shaoxing Hospital of China Medical UniversityShaoxing 312000Zhejiang ProvinceChina Department of Emergency Medicine Shaoxing Hospital of China Medical UniversityShaoxing 312000Zhejiang ProvinceChina Department of Emergency Medicine Zhuji People's Hospital of Zhejiang ProvinceZhuji 311800Zhejiang ProvinceChina

BACKGROUND Epidemiological studies have found that the prevalence of chronic heart failure in China is 0.9%,the number of people affected is more than 4 million,and the 5-year survival rate is even lower than that of malignant *** To determine the impact of WeChat platform-based health management on severe chronic heart failure patients’health and self-management *** A total of 120 patients suffering from chronic heart failure with cardiac function grade III-IV,under the classification of the New York Heart Association,were admitted to our hospital in May *** January 2020,they were divided into two groups:A control group(with routine nursing intervention)and an observation group(with WeChat platform-based health management intervention).Changes in cardiac function,6-min walking distance(6MWD),high-sensitivity cardiac troponin(hs-cTnT),and N-terminal pro B-type natriuretic peptide(NT-proBNP)were detected in both *** self-Care Ability scale(EsCA)score,Minnesota Living with Heart Failure Questionnaire score,and compliance score were used to evaluate self-management ability,quality of life,and compliance of the two *** a follow-up period of 12 mo,the occurrence of cardiovascular adverse events in both the groups was *** The left ventricular ejection fraction,stroke output,and 6MWD increased,and the hs-cTnT and NT-proBNP decreased in both the groups,as compared to those before the ***,cardiac function during the 6MWD,hs-cTnT,and NT-proBNP improved significantly in the observation group after intervention(Pscores of self-care responsibility,self-concept,self-care skills,and selfcare health knowledge in the observation group were higher than those of the control group before intervention,and their EsCA scores were significantly improved after intervention(Psota heart failure quality of life(LiHFe)scores of physical restriction,disease symptoms,psychological emotion,social relat

关键词： WeChat platform Health management severe chronic heart failure self-care capacity Cardiac function Adverse cardiovascular events

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A new luciferase immunoprecipitation system assay provided serological evidence for missed diagnosis of severe fever with thrombocytopenia syndrome

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Virologica sinica 2022年第1期37卷 107-114页

作者： shengyao Chen Minjun Xu Xiaoli Wu Yuan Bai Junming shi Min Zhou Qiaoli Wu shuang Tang Fei Deng Bo Qin shu shen state Key Laboratory of Virology and National Virus Resource Center Wuhan Institute of VirologyChinese Academy of SciencesWuhan430071China shaoxing Women and Children©s hospital No.305 East Street RoadShaoxing312000China University of Chinese Academy of sciences Beijing100049China shaoxing people©s hospital Zhejiang University School of MedicineShaoxing312000China

severe fever with thrombocytopenia syndrome(sFTs),caused by sFTs virus(sFTsV)infection,was first reported in 2010 in China with an initial fatality of up to 30%.The laboratory confirmation of sFTsV infection in terms of detection of viral RNA or antibody levels is critical for sFTs diagnosis and *** this study,a new luciferase immunoprecipitation system(LIPs)assay based on p REN2 plasmid expressing sFTsV NP gene and tagged with Renilla luciferase(Rluc),was established and used to investigate the levels of antibody responses to *** 464 serum samples from febrile patients were collected in the hospital of shaoxing City in Zhejiang Province in *** results showed that 82 of the 464 patients(17.7%)had antibody response to sFTsV,which were further supported by immunofluorescence assays(IFAs).Further,q RT-PCR and microneutralization tests showed that among the 82 positive cases,15 patients had viremia,10 patients had neutralizing antibody,and one had both(totally 26 patient).However,none of these patients were diagnosed as sFTs in the hospital probably because of their mild symptoms or subclinical *** the results indicated that at least the 26 patients having viremia or neutralizing antibody were the missed diagnosis of sFTs *** findings suggested the occurrence of sFTs and the sFTs incidence were higher than the reported level in shaoxing in 2019,and that LIPs may provide an alternative strategy to confirm sFTsV infection in the laboratory.

关键词： severe fever with thrombocytopenia syndrome(sFTs) severe fever with thrombocytopenia syndrome virus(sFTsV) Luciferase immunoprecipitation systems(LIPs) shaoxing serological evidence Missed diagnosis

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Assessment of outcomes of hearing and speech rehabilitation in children with cochlear implantation

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Journal of Otology 2019年第2期14卷 57-62页

作者： shaofeng Liu Fang Wang Peipei Chen Na Zuo Cheng Wu Jun Ma Jingjiang Huang Chuanxi Wang Department of Otolaryngology-Head and Neck surgery Yijisan Hospital of Wanan Medical CollegeWuhuAnhui241001China Department of Otolaryngology Shaoxing People's HospitalShaoxingZhejiang312000China

Objectives:This study aimed to assess the effect of hearing and speech rehabilitation in patients with Nurotron■cochlear ***:Ninety-eight paediatric patients with bilateral severe-to-profound sensorineural deafness who received cochlear implantation were divided into three groups according to age:group A(≤3 years),group B(4-7 years),and group C(8-16 years).All patients were followed up for one year for hearing and speech performance after the *** comprehensive Auditory Perception Assessment,MAIs,CAP and sIR hearing and speech assessments and rating materials were used for assessment before the surgery and at 3,6,and 12 months after implant ***:The scores of patients in the open-set speech assessment,Chinese Auditory Perception Assessment,MAIs,CAP and sIR significantly improved after cochlear implantation in all age *** younger the age at implantation,the better the ***,the hearing and speech performance of cochlear implant recipients gradually improved with the extension of rehabilitation ***:Nurotron■Venus^TM cochlear implantation can improve the hearing and speech performance of patients with bilateral severe-to-profound sensorineural deafness.

关键词： Hearing loss sensorineural Cochlear implant Rehabilitation speech perception Assessment

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Analysis and validation of diagnostic biomarkers and immune cell infiltration characteristics in pediatric sepsis by integrating bioinformatics and machine learning

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World Journal of Pediatrics 2023年第11期19卷 1094-1103页

作者： Wen‑Yuan Zhang Zhong‑Hua Chen Xiao‑Xia An Hui Li Hua‑Lin Zhang shui‑Jing Wu Yu‑Qian Guo Kai Zhang Cong‑Li Zeng Xiang‑Ming Fang Department of Anesthesiology and Intensive Care School of MedicineThe First Affiliated HospitalZhejiang UniversityQingChun Road 79Hangzhou 310003China Department of Anesthesiology Shaoxing People’s Hospital(Shaoxing HospitalZhejiang University School of Medicine)Shaoxing 312000China Department of Anesthesiology Vagelos College of Physicians and SurgeonsColumbia UniversityNew YorkNYUSA

Background Pediatric sepsis is a complicated condition characterized by life-threatening organ failure resulting from a dysregulated host response to infection in *** is associated with high rates of morbidity and mortality,and rapid detection and administration of antimicrobials have been *** objective of this study was to evaluate the diagnostic biomarkers of pediatric sepsis and the function of immune cell infiltration in the development of this *** Three gene expression datasets were available from the Gene Expression Omnibus ***,the differentially expressed genes(DEGs)were found with the use of the R program,and then gene set enrichment analysis was carried ***,the DEGs were combined with the major module genes chosen using the weighted gene co-expression *** hub genes were identified by the use of three machine-learning algorithms:random forest,support vector machine-recursive feature elimination,and least absolute shrinkage and selection *** receiver operating characteristic curve and nomogram model were used to verify the discrimination and efficacy of the hub *** addition,the inflammatory and immune status of pediatric sepsis was assessed using cell-type identification by estimating relative subsets of RNA transcripts(CIBERsORT).The relationship between the diagnostic markers and infiltrating immune cells was further *** Overall,after overlapping key module genes and DEGs,we detected 402 overlapping *** pediatric sepsis diagnostic indicators,CYsTM1(AUC=0.988),MMP8(AUC=0.973),and CD177(AUC=0.986)were investigated and demonstrated statistically significant differences(Pstic efficacy in the validation *** indicated by the immune cell infiltration analysis,multiple immune cells may be involved in the development of pediatric ***,all diagnostic characteristics may correlate with immune cells to varying *** The candida

关键词： Paediatric sepsis Bioinformatics Machine-Learning Biomarkers Immune cell infiltration

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Breast cancer-derived exosomes transmit lncRNA sNHG16 to induce CD73+γδ1 Treg cells

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signal Transduction and Targeted Therapy 2020年第1期5卷 1953-1966页

作者： Chao Ni Qing-Qing Fang Wu-Zhen Chen Jin-Xing Jiang Zhou Jiang Jun Ye Ting Zhang Liu Yang Fan-Bo Meng Wen-Jie Xia Miaochun Zhong Jian Huang Department of Breast surgery Second Affiliated HospitalZhejiang UniversityHangzhouZhejiang 310009China Key Laboratory of Tumour Microenvironment and Immune Therapy of Zhejiang Province Second Affiliated HospitalZhejiang UniversityHangzhouZhejiang 310009China Key Laboratory of Tumour Molecular Diagnosis and Individualized Medicine of Zhejiang Province Zhejiang Provincial People’s HospitalPeople’s Hospital of Hangzhou Medical CollegeHangzhouZhejiang 310014China Department of Thyroid and Breast surgery Affiliated Hospital of Shaoxing UniversityShaoxingZhejiang 312099China Department of Thyroid and Breast surgery Zhejiang Provincial People’s HospitalPeople’s Hospital of Hangzhou Medical CollegeHangzhouZhejiang 310014China

γδT cells have been reported to exert immunosuppressive functions in multiple solid malignant diseases,but their immunosuppressive functional subpopulation in breast cancer(BC)is still ***,we collected 40 paired BC and normal tissue samples from Chinese patients for ***,we showed thatγδT1 cells comprise the majority of CD3+T cells in BC;next,we found that CD73+γδT1 cells were the predominant regulatory T-cell(Treg)population in BC,and that their prevalence in peripheral blood was also related to tumour *** addition,CD73+γδT1 cells exert an immunosuppressive effect via adenosine *** also found that BC could modulate CD73 expression onγδT cells in a non-contact *** microarray analysis and functional experiments indicated that breast tumour cell-derived exosomes(TDEs)could transmit lncRNA sNHG16,which upregulates CD73 expression,to Vδ1 T *** the mechanism,sNHG16 served as a ceRNA by sponging miR-16–5p,which led to the derepression of its target gene sMAD5 and resulted in potentiation of the TGF-β1/sMAD5 pathway to upregulate CD73 expression in Vδ1 T *** results showed that the BC-derived exosomal sNHG16/miR-16–5p/sMAD5-regulatory axis potentiates TGF-β1/sMAD5 pathway activation,thus inducing CD73 expression in Vδ1 T *** results first identify the significance of CD73+Vδ1 Tregs in BC,and therapy targeting this subpopulation or blocking TDEs might have potential for BC treatment in the future.

关键词： CD73 cancer Treg

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MicroRNAs and long non-coding RNAs in liver surgery:Diagnostic and therapeutic merits

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Hepatobiliary & Pancreatic Diseases International 2020年第3期19卷 218-228页

作者： Zhi-Qiu Hu Yi Lu Di Cui Chen-Yang Ma su shao Ping Chen Ran Tao Jian-Jun Wang Department of surgery Minhang HospitalFudan UniversityShanghai 201199China Department of Hepatobiliary-Pancreatic&Minimally Invasive surgery Affiliated Zhejiang Provincial People’s HospitalHangzhou Medical SchoolHangzhou 310014China Center for Clinical Medical Research Affiliated Zhejiang Provincial People’s HospitalHangzhou Medical SchoolHangzhou 310014China Department of General surgery Chun’an 1st People’s HospitalHangzhou 311700China Department of Obstetrics and Gynecology Shaoxing 2nd HospitalShaoxing 312000China

Background:Hepatectomy and liver transplantation(LT)are the two most commonly performed surgical procedures for various hepatic *** RNA(mi RNA)and long non-coding RNA(lnc RNA)have been gradually unveiled their roles as either biomarkers for early diagnosis or potentially therapeutic tools to manipulate gene expression in many disease *** review aimed to discuss the effects of mi RNA or lnc RNA in the hepatectomy and LT *** sources:We did a literature search from 1990 through January 2018 to summarize the currently available evidence with respect to the effects of mi RNA and lnc RNA in liver regeneration after partial hepatectomy,as well as their involvement in several key issues related to LT,including ischemia-reperfusion injury,allograft rejection,tolerance,recurrence of original hepatic malignancies,***:Certain mi RNAs and lnc RNAs are actively involved in the regulation of various aspects of liver resection and *** the process of liver regeneration after hepatectomy,the expression of mi RNAs and lnc RNAs shows dynamic ***:It is now clear that mi RNAs and lnc RNAs orchestrate in various aspects of the pathophysiological process of LT and *** understanding of the underlying mechanism and future clinical trials may strengthen their positions as either biomarkers or potential therapeutic targets in the management of complications after liver surgery.

关键词： Hepatectomy Liver transplantation microRNA Long non-coding RNA Ischemia-reperfusion injury Rejection

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