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HIV-infected Female Sex Workers High Risk Behavior and Attitude Changes in Kaiyuan City, Yunnan Province, China

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Biomedical and Environmental Sciences 2014年第6期27卷 444-452页

作者： DING Guo Wei HSI Jenny H LIU Hui Xin SU Ying Ying WANG Jun Jie BAI Jun WANG Gui Xiang WANG Hai Bo DONG Rui Ling WANG Ning National center for AIDS/STD Control and Prevention Chinese Center for Disease Control and Prevention Kaiyuan City center for Disease Control and Prevention Chinese center for Disease Control and Prevention Shenzhen international travel healthcare center

Objective To investigate the attitude and sexual behavior status and change among HIV positive female workers in entertainment sites in Kaiyuan city, Yunnan province, China. The key information should be applied in the integrated intervention program in future. Methods A cohort survey among HIV positive female workers was conducted during 12 months, between 2010 and 2012. All the risk sexual behavior and attitude were collected for assessment for the potential secondary transmission to sexual partners. Results Of 99 HIV positive women who sell sex in Kaiyuan city, 99 participated in the survey at baseline, 80, 80, 75, and 75 at 3-, 6-, 9-, and 12-month follow-ups. The percentage of participants who reported consistently used condoms in the last one month ranged between 94.5% and 95.5%. The client volume in the last one month, income per sex and age group were significant related with non-insistent condom use with their clients. Conclusion It was suggested that integrated intervention program package should include 100 percent condom use promotion for the HIV positive FSW with all sexual partners, and also, include socially support involved.

关键词： HIV-infected Female sex workers Sexual behavior Change

Gene characterization and phylogenetic analysis of four mitochondrial genomes in Caenogastropoda

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Acta Oceanologica Sinica 2024年第2期43卷 137-150页

作者： Jiangyong Qu Wanqi Yang Xindong Teng Li Xu Dachuan Zhang Zhikai Xing Shuang Wang Xiumei Liu Lijun Wang Xumin Wang College of Life Science Yantai UniversityYantai 264005China Qingdao international travel healthcare center Qingdao 266071China Qingdao Dagang Customs Qingdao 266071China

Caenogastropoda is a highly diverse group,containing~60%of all existing *** in this subclass predominantly inhabit marine environments and have a high ecological and economic *** to the increase in relevant phylogenetic studies,our understanding of between species relatedness in Caenogastropoda has ***,the biodiversity,taxonomic status,and phylogenetic relationships of this group remain *** the present study,we performed next-generation sequencing of four complete mitochondrial genomes from three families(Buccinidae,Columbellidae,and Cypraeidae)and the four mitogenomes were classical circular structures,with a length of 16177 bp in Volutharpa ampullacea,16244 bp in Mitrella albuginosa,16926bp in Mauritia arabica asiatica and 15422 bp in Erronea *** composition analysis indicated that whole sequences were biased toward A and *** compared them with 171 complete mitochondrial genomes of *** phylogenetic relationship of Caenogastropoda derived from Maximum Likelihood(ML)and Bayesian Inference(BI)trees constructed based on CDS sequences was consistent with the results of traditional morphological analysis,with all three families showing close *** study supported Caenogastropoda at the molecular level as a separate clade of *** to our divergence time estimations,Caenogastropoda was formed during the middle Triassic period(~247.2–237 Ma).Our novel mitochondrial genomes provide evidence for the speciation of Caenogastropoda in addition to elucidating the mitochondrial genomic evolution of this subclass.

关键词： mitochondrial genome phylogenetic analysis Caenogastropoda

Moxa Wool in Different Purities and Different Growing Years Measured by Terahertz Spectroscopy

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Plant Phenomics 2022年第1期4卷 235-246页

作者： Yongni Shao Di Zhu Yutian Wang Zhi Zhu Wenchao Tang Zhengan Tian Yan Peng Yiming Zhu Terahertz Technology Innovation Research Institute Terahertz Spectrum and Imaging Technology Cooperative Innovation CenterShanghai Key Lab of Modern Optical SystemUniversity of Shanghai for Science and TechnologyShanghai 20009China shanghai Institute of Intelligent Science and Technology Tongji UniversityShanghai 200092China School of Acupuncture-Moxibustion and Tuina Shanghai University of Traditional Chinese MedicineChina shanghai international travel healthcare center Shanghai Customs District P.R.200335China

Moxa wool is a traditional Chinese herbal medicine,which can warm channels to dispel *** present,there is no unified index to evaluate the purity and growing years of moxa wool in the *** is one of the effective substances in the volatile oil of moxa ***,we characterize the purity and growing years of moxa wool by studying *** chromatography-mass spectrometry(GC-MS)and high-performance liquid chromatography(HPLC)are the methods for monitoring terpineol at present,all of which have defects of complicated procedures.

关键词： wool purity unified

Study on the structure characterization and moisturizing effect of Tremella polysaccharide fermented from GCMCC5.39

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Food Science and Human Wellness 2021年第4期10卷 471-479页

作者： Meng Yang Zilong Zhang Yan He Chengliang Li Jinmei Wang Xia Ma School of Perfume and Aroma Technology Shanghai Institute of TechnologyShanghai 201418China shanghai international travel healthcare center Shanghai Customs District P.R.ChinaShanghai 200335China Laibo Pharmaceutical Technology(shanghai)Co.Ltd Shanghai 201418China National R&D center for Edible Fungus Processing Technology Henan UniversityKaifeng 475004China

The structure and moisture retention of Tremella polysaccharide fermented from GCMCC5.39(FTP)were *** UV,infrared spectrum analysis,HPAEC-PAD,HPSEC and 1 D NMR analysis,the composition of the purifi ed FTP was *** ed components of fermented Tremella polysaccharide(FTPS)was made of galactose,mannose,glucose,galactosmine,glucosamine,and contain a large amount of hydroxyl,carbonyl and amino *** wasα-neutral pyranose without uronic ***-1 and FTPS-2 were obtained after purifi cation by DEAE-Sepharose Fast Flow *** molecular weights of FTPS-1 and FTPS-2 were 25722 and 177263 ***-2 had a better ability to prevent moisture loss,and the optimal moisture retention period was 0–4 ***-2 could signifi cantly increase the moisture content of the skin epidermis and showed a dose-concentration *** effect of FTPS-2 on the expression of different moisturizing genes was evaluated in a human skin keratinocyte *** results showed that FTPS-2 has no cytotoxicity,and could signifi cantly promote AQP3,TGM1,CASP14,HYAL2,FLG gene expression level in HaCaT *** has the most signifi cant infl uence at HYAL2 protein expression on 50μg/mL.

关键词： Polysaccharide Tremella spp. Structure identifi cation Moisturizing

Assessment of the Potential for Cross-Border Transmission of Infectious Diseases via Commercial Air travel—shanghai Municipality,China,2019-2023

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China CDC weekly 2024年第42期6卷 1093-1097页

作者： Weimin Hao Jieyan Gu Yujue Wang Li Wang Liguang Hou Ting Wang Wei Zhang Yi Zhang Na He Zhengan Tian Department of Epidemiology School of Public HealthFudan UniversityShanghaiChina Division of Health Quarantine Shanghai Customs DistrictShanghaiChina Division of Enterprise Management Shanghai Customs DistrictShanghaiChina Hongqiao Airport Customs House Shanghai Customs DistrictShanghaiChina Pudong international Airport Customs House Shanghai Customs DistrictShanghaiChina shanghai Institute of Infectious Disease and Biosecurity Fudan UniversityShanghaiChina shanghai international travel healthcare center ShanghaiChina

What is already known about this topic?Despite the end of coronavirus disease 2019(COVID-19)as a public health emergency of international concern(PHEIC),the changes in risks of travel-related infectious diseases and their impact on global health quarantine still call for great *** is added by this report?Our study indicated that the pattern of changes in the positive detection rate of arrivals was basically in line with the disease incidence in countries from which travel was *** are the implications for public health practice?Accurate risk assessment at entry-exit ports can significantly enhance the efficiency of targeting highrisk international arrivals,especially in the early stages of epidemic control and prevention when available data are insufficient.

关键词： prevention travel diseases

Construction of Lentivirus-Based Reference Material for RT-PCR Detection of Middle East Respiratory Syndrome Coronavirus and Its Application in External Quality Assessment

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Advances in Microbiology 2018年第7期8卷 506-518页

作者： Donggen Zhou Jie Luo Ningbo international travel healthcare center Ningbo China

Short Retraction Notice The paper is due to conflicts of interest. This article has been retracted to straighten the academic record. In making this decision the Editorial Board follows COPE's Retraction Guidelines. Aim is to promote the circulation of scientific research by offering an ideal research publication platform with due consideration of internationally accepted standards on publication ethics. The Editorial Board would like to extend its sincere apologies for any inconvenience this retraction may have caused. The full retraction notice in PDF is preceding the original paper which is marked "RETRACTED".

关键词： MERS-CoV

Comparative Analysis of the 5’ Flanking Region in <i>Arabidopsis</i>and <i>O. sative</i>RP Genes Revealed Conserved and Divergent Regulating Mechanisms

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American Journal of Plant Sciences 2019年第6期10卷 1090-1101页

作者： Donggen Zhou Jie Luo Ningbo international travel healthcare center Ningbo China

Ribosome is one of the most abundant organelles in all living cells and plays a crucial role in cell growth. Synthesis of ribosomal components is tightly related with the change of growth conditions. We have comparatively analyzed the 5’ flanking region of ribosomal protein (RP) genes in Arabidopsis and O. sativa. In both Arabidopsis and O. sativa, there are two putative transcriptional factor binding motifs (telo box and site II elements) overrepresented in the proximal promoter region with a strong positional bias in most of the RP genes, which suggests the conserved mechanism of transcription-level control in RP genes of these two organisms. Tri-nucleotide repeats motif CTT and CCG were also common in 5’ flanking region of RP genes in Arabidopsis and O. sativa. However, we only found CCG repeat motif was enriched in O. sativa RP genes and most of them were clustered in the 5’ UTR region. This finding reveals molecular mechanism for divergent regulation of RP genes in Arabidopsis and O. sativa, and gives the possible clue to the mechanism of controlling O. sativa RP genes expression at the translation level.

关键词： Ribosome Motif Genes Expression Mechanism

Development and Validation of Multiplex One-Step Real-Time TaqManqRT-PCR Assays for Detection and Quantification of Arboviral Encephalitis Viruses

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Advances in Microbiology 2018年第7期8卷 519-557页

作者： Donggen Zhou Jie Luo Ningbo international travel healthcare center Ningbo China

Arboviral encephalitis is a group of animal and human illness that is mostly caused by several distinct families of viruses including orthobunya virus, phlebovirus, flaviviruses, and the alphaviruses. Although specific signs and symptoms vary by the type of central nervous system (CNS), initial signs and symptoms are very similar. Therefore rapid immunologic and molecular tools for differential diagnosis of arboviral encephalitis viruses are important for effective case management and control of the spread of encephalitis. The qRT-PCR assay, especially multiplex PCR, has the potential to produce considerable savings in time and resources in the laboratory detection. Meanwhile, the use of IC can prevent false negatives effectively by monitoring the processes of nucleic acid extraction and amplification. This report describes the development of a panel of internally controlled multiplex one-step real-time RT-PCR assays in which two virus specific-probe sets were used in the same reaction for the detection of 15 species arboviral encephalitis viruses: the comparative sensitivity of multiplex one-step qRT-PCR assays to single plex one-step qRT-PCR assays as well as one-step RT-PCR assays for detection of each viral species. And total of 150 human serum samples were detected to evaluate the multiplex one-step qRT-PCR assays. These multiplex one-step real-time RT-PCR assays with IC were evaluated in terms of sensitivity, linearity, precision, specificity, and also field samples including serum and vector. These assays can detect and differentiate arboviral encephalitis viruses by high throughput, sensitive, and specific way. It is useful for clinical management and outbreak control of arboviral encephalitis viruses and vector surveillance.

关键词： Multiplex One-Step Real-Time TaqManqRT-PCR Assays Arboviral Encephalitis Viruses Internal Control

General methods to obtain and analyze the complete mitochondrial genome of aphid species: Eriosoma lanigerum(Hemiptera: Aphididae) as an example

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Zoological Systematics 2016年第2期41卷 123-132页

作者： Yuan Wang Liyun Jiang Yongjie Liu Jing Chen Gexia Qiao Shaanxi international travel healthcare center Shaanxi Entry-Exit Inspection and Quarantine Bureau Key Laboratory of Zoological Systematics and Evolution Institute of Zoology Chinese Academy of Sciences

Insect mitochondrial （mt） genomes are of great interest in researching on molecular evolution, phylogenetics and population genetics. Aphidoidea have about more than 5 000 known species including some agricultural, forestry and horticultural pests. However, only nine complete mt genomes have been previously released in this insect group since the first one, Schizaphis graminum （Hemiptera： Aphididae： Aphidinae） in 2004. Herein, we present the complete mitochondrial genome of Eriosoma lanigerum, the first species from the subfamily Eriosomatinae. This study takes this species as an example, combing the research during past 12 years, and concludes the general methods to obtain, analyze and annotate the complete mitochondrial genome of aphid species.

关键词： Mitochondrial genome Eriosoma lanigerum methods aphids