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Detection of anti-preS1 antibodies for recovery of hepatitis B patients by immunoassay

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World Journal of Gastroenterology 2002年第2期8卷 276-281页

作者： Jun Wei Guang-Di Li Yuan Wang Zu-Chuan Zhang,Institute of Biochemsitry and Cell Biology,shanghai institutes for biological sciences,Chinese Academy of sciences,shanghai 200031,China Yu-Qin Wang Zhi-Meng Lu,Department of Clinical virology,Rui-Jin Hospital,shanghai Second Medical University 200025,shanghai,China Institute of Biochemsitry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China.

AIM: To establish a convenient immunoassay method based on recombinant antigen preS1(21-119aa) to detect anti-preS1 antibodies and evaluate the clinical significance of antibodies in hepatitis B. METHODS: The expression plasmid pET-28a-preS1 was constructed, and a large quantity of preS1(21-119aa) fragment of the large HBsAg protein was obtained. The preS1 fragment purified by Ni(2+)-IDA affinity chromatography was used as coated antigen to establish the indirect ELISA based on streptavidin-biotin system for detection of the anti-preS1 antibodies in sera from HBV-infected patients. For follow-up study, serial sera were collected during the clinical course of 21 HBV-infected patients and anti-preS1 antibodies, preS1 antigen, HBV-DNA and other serological HBV markers were analyzed. RESULTS: preS1(21-119aa) fragment was highly expressed from the plasmid pET-28a-preS1 in a soluble form in *** (30mg.L(-1)), and easily purified to high purity over 90% by one step of Ni(2+)-IDA-sepharose 6B affinity chromatography. The purity and antigenicity of the purified preS1(21-119aa) protein was determined by 150g.L(-1) SDS-PAGE, Western blot and a direct ELISA. Recombinant preS1(21-119aa) protein was successfully applied in the immunoassay which could sensitively detect the anti-preS1 antibodies in serum specimens of acute or chronic hepatitis B patients. Results showed that more than half of 19 acute hepatitis B patients produced anti-preS1 antibodies during recovery of the disease, however, the response was only found in a few of chronic patients. In the clinical follow-up study of 11 patients with anti-preS1 positive serological profile, HBsAg and HBV-DNA clearance occurred in 6 of 10 acute hepatitis B patients in 5-6 months, and seroconversion of HBeAg and disappearance of HBV-DNA occurred in 1 chronic patients treated with lavumidine, a antiviral agent. CONCLUSION: The high-purity preS1(21-119aa) coated antigen was successfully prepared by gene expression and affinity chrom

关键词： Amino Acid Sequence Antibodies Base Sequence Genetic Vectors Hepatitis B Hepatitis B Surface Antigens Humans Immunoassay Molecular Sequence Data Peptide Fragments Protein Precursors Recombinant Fusion Proteins Research Support, Non-U.S. Gov't Viral Envelope Proteins

Axonal bleb recording

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Neuroscience Bulletin 2012年第4期28卷 342-350页

作者： Wenqin Hu Yousheng Shu Institute of Neuroscience State Key Laboratory of NeuroscienceShanghai Institutes for Biological SciencesChineseAcademy of SciencesShanghai 200031China

Patch-clamp recording requires direct accessibility of the cell membrane to patch pipettes and allows the investigation of ion channel properties and functions in specific cellular *** cell body and relatively thick dendrites are the most accessible compartments of a neuron,due to their large diameters and therefore great membrane surface ***,axons are normally inaccessible to patch pipettes because of their thin structure;thus studies of axon physiology have long been hampered by the lack of axon recording ***,a new method of patchclamp recording has been developed,enabling direct and tight-seal recording from cortical *** recordings are performed at the enlarged structure（axonal bleb） formed at the cut end of an axon after slicing *** method has facilitated studies of the mechanisms underlying the generation and propagation of the main output signal,the action potential,and led to the finding that cortical neurons communicate not only in action potential-mediated digital mode but also in membrane potential-dependent analog mode.

关键词： axon patch-clamp recording ion channel action potential

Comparative analysis of rapamycin biosynthesis clusters between Actinoplanes sp. N902-109 and Streptomyces hygroscopicus ATCC29253

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Chinese Journal of Natural Medicines 2015年第2期13卷 90-98页

作者： HUANG He REN Shuang-Xi YANG Sheng HU Hai-Feng shanghai Institute of Pharmaceutical Industry Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences

The present study was designed to identify the difference between two rapamycin biosynthetic gene clusters from Streptomyces hygroscopicus ATCC29253 and Actinoplanes sp. N902-109 by comparing the sequence and organization of the gene clusters. The biosynthetic gene cluster for rapamycin in Streptomyces hygroscopicus ATCC29253 was reported in 1995. The second rapamycin producer, Actinoplanes sp. N902-109, which was isolated in 1995, could produce more rapamycin than Streptomyces hygroscopicus ATCC29253. The genomic map of Actinoplanes sp. N902-109 has been elucidated in our laboratory. Two gene clusters were compared using the online software anti-SMASH, Glimmer 3.02 and Subsystem Technology(RAST). Comparative analysis revealed that the organization of the multifunctional polyketide synthases(PKS) genes: Rap A, RapB, RapC, and NRPS-like RapP were identical in the two clusters. The genes responsible for precursor synthesis and macrolactone modification flanked the PKS core region in N902-109, while the homologs of those genes located downstream of the PKS core region in ATCC29253. Besides, no homolog of the gene encoding a putative type II thioesterase that may serve as a PKS "editing" enzyme accounted for over-production of rapamycin in N902-109, was found in ATCC29253. Furthermore, no homologs of genes rapQ(encoding a methyltransferase) and rap G in N902-109 were found in ATCC29253, however, an extra rap M gene encoding methyltransferase was discovered in ATCC29253. Two rapamycin biosynthetic gene clusters displayed overall high homology as well as some differences in gene organization and functions.

关键词： Rapamycin Actinoplanes Biosynthetic gene cluster Comparative analysis

Effects of cisplatin on telomerase activity and telomere length in BEL-7404 human hepatoma cells

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Cell Research 2002年第1期12卷 55-62页

作者： Ru GANG ZHANG, Ru PING ZHANG, XING WANG WANG, HONG XIE Department of Biotherapy, Institute of Biochemistry and Cell Biology, shanghai institutes for biological sciences, the Chinese Academy of sciences, shanghai 200031, China Department of Biotherapy Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences Shanghai China

Telomerase activity was inhibited in a dose and time-dependent manner with the treatment of cisplatin for 24, 48, or 72 h in a concentration ranged from 0.8 to 50 1uM in BEL-7404 human hepatoma cells. There were no changes in expression pattern of three telomerase subunits, its catalytic reverse transcriptase subunit (hTERT), its RNA component (hTR) or the associated protein subunit (TP1), after cisplatin treated for 72 h with indicated concentrations. Mean telomere lengths were decreased by the cisplatin treatment. Cell growth inhibition and cell cycle accumulation in G2/M phase were found to be correlated with telomerase inhibition in the present study, but percentages of cell apoptosis did not change markedly during the process.

关键词： Telomerase telomere cisplatin hepatocellular carcinoma cell cycle cell growth.

Bitter but tasty cucumber

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National Science Review 2015年第2期2卷 129-130页

作者： Xiao-Ya Chen Institute of Plant Physiology and Ecology Shanghai Institutes for Biological SciencesChinese Academy of Sciences

Plant natural products consisting mainly of phenolic compounds,alkaloids and terpenoids,are valuable for both plants and *** a breakthrough has been made in elucidation of biosynthesis of cucurbitacins[1],a class of h... 详细信息

关键词： gene

Synthesis and Insulin-sensitizing Activity of a Series of 2-Benzyl-1,3-dicarbonyl Derivatives

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Chinese Journal of Chemistry 2003年第4期21卷 365-368页

作者：汤磊冷颖王火权冯颖杨玉社嵇汝运 State Key Laboratory of Drug Research Shanghai Institute of Materia MedicaShanghai Institutes for Biological SciencesChinese Academy of Sciences State Key Laboratory of Drug Research Shanghai Institute of Materia MedicaShanghai Institutes for Biological SciencesChinese Academy of Sciences State Key Laboratory of Drug Research hanghai Institute of Materia MedicaShanghai Institutes for Biological SciencesChinese Academy of Sciences

A series of 2 benzyl 1,3 dicabonyl derivatives was synthesized. Their insulin sensitizing activity was evaluated in 3T3 L1 preadipocyte cells. Compounds 3, 26 and 27 were found to possess strong insulin sens... 详细信息

关键词： insulin sensitizing activity 3T3 L1 cells type 2 diabetes

Novel Secondary Structure of Calcitonin in Solid State as Revealed by Circular Dichroism Spectroscopy

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Chinese Journal of Chemistry 2002年第7期20卷 697-698页

作者：杜海宁丁金国崔大敷胡红雨 Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China

The solid-state circular dichroic study reveals that salmon calci-toninpresents a typical α-helical structure while human calci-tonin appears to form a β-sheet in solidstate, although both of them adopt random coil ... 详细信息

关键词： calcitonin circular dichroism solid state secondary structure

Evolution from genetics to phenotype： reinterpretation of NSCLC plasticity, heterogeneity, and drug resistance

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Protein & Cell 2017年第3期8卷 178-190页

作者： Yingjiao Xue Shenda Hou Hongbin Ji Xiangkun Han Key Laboratory of Systems Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China Innovation Center for Cell Signaling Network Shanghai Institutes for Biological Sciences Chinese Academy of Sciences_ Shanghai 200031 China CAS Center for Excellence in Molecular Cell Science Institute of Biochemistry and Cell Biology Shanghai Institutes forBiological Sciences Chinese Academy of Sciences Shanghai 200031 China School of Life Science and Technology Shanghai Tech University Shanghai 200120 China Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of SciencesShanghai 200031 China

Lung cancer is the leading cause of cancer-related deaths worldwide. Targeted therapy is beneficial in most cases, but the development of drug resistance stands as an obstacle to good prognosis. Multiple mechanisms were explored such as genetic alterations, activation of bypass signaling, and phenotypic transition. These intrinsic and/or extrinsic dynamic regulations facilitate tumor cell survival in meeting the demands of signaling under different stimulus. This review introduces lung cancer plasticity and heterogeneity and their correlation with drug resistance. While cancer plasticity and heterogeneity play an essential role in the development of drug resistance, the manipulation of them may bring some inspirations to cancer prognosis and treatment. That is to say, lung cancer plasticity and heterogeneity present us with not only challenges but also opportunities.

关键词： lung cancer plasticity heterogeneity drugresistance phenotypic transition

Whereto the mega brain projects?

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National Science Review 2014年第1期1卷 12-14页

作者： Mu-ming Poo Institute of Neuroscience Shanghai Institutes for Biological Sciences Chinese Academy of Sciences

The mega brain projects announced by the US President and European Commission in2013 ofer new initiatives for developing innovative neurotechnology,but the prospect of efective therapies for brain disease remains *** his 2013 State of the Union address President Obama of the United States shook the global neuroscience community by announcing a 10-year,3 billion dollar project,the‘Brain Activity Map’,that aims to‘image every spike

关键词： Whereto the mega brain projects