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Operando investigation of particle re-entrainment mechanism in electrostatic capture process on the lab-on-a-chip

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Journal of Environmental Sciences 2024年第2期136卷 337-347页

作者： Yong Zhu Yikun Zhang Xiaoyong Yang Shanlong Tao Mingxia Chen Wenfeng Shangguan research center for Combustion and Environmental Technology School of Mechanical EngineeringShanghai Jiao Tong UniversityShanghai 200240China

Inhalable particle is a harmful air pollutant that causes a significant threat to people's health and ecological environments,which should be removed to purify air,but there exists limited removal efficiency due to particle re-entrainment.Here,Operando observation system based on microscopic visualization method is developed to make in situ test of particle migration,deposition and re-entrainment characteristics on a lab-on-a-chip to achieve the investigation in micro-level scale.The deposition evolution of charged particles is recorded in electric field region intuitively,which confirms the fracture of particle chain occurs during the growth process of deposited particles.It captures the instantaneous process that a larger particle with micron size due to the coagulation of submicron particles fractures from main body of the particle chain for the first time.The analysis of migration behavior of a single submicron particle near electrode surface demonstrates the direct influence of drag force on the fracture of particle chain.This work is the first-time visualization of dynamic process and mechanism elucidation of particle re-entrainment at the micron level,and the findings will provide the theory support for the particle re-entrainment mechanism and bring inspires of enhancing capture efficiency of inhalable particle.

关键词： Inhalable particle Re-entrainment mechanism Operando observation Lab-on-a-chip Capture efficiency

Boosting Hydrogen Storage Performance of MgH_(2) by Oxygen Vacancy-Rich H-V_(2)O_(5) Nanosheet as an Excited H-Pump

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Nano-Micro Letters 2024年第8期16卷 398-416页

作者： Li Ren Yinghui Li Zi Li Xi Lin Chong Lu Wenjiang Ding Jianxin Zou National engineering research center of Light Alloys Net Forming&State Key Laboratory of Metal Matrix Composites Shanghai Jiao Tong UniversityShanghai 200240People’s Republic of China shanghai engineering research center of Mg Materials and Applications&School of Materials Science and engineering Shanghai Jiao Tong UniversityShanghai 200240People’s Republic of China center of Hydrogen Science Shanghai Jiao Tong UniversityShanghai 200240People’s Republic of China Instrumental Analysis center of SJTU Shanghai Jiao Tong UniversityShanghai 200240People’s Republic of China

MgH_(2) is a promising high-capacity solid-state hydrogen storage material,while its application is greatly hindered by the high desorption temperature and sluggish kinetics.Herein,intertwined 2D oxygen vacancy-rich V_(2)O_(5) nanosheets(H-V_(2)O_(5))are specifically designed and used as catalysts to improve the hydrogen storage properties of MgH_(2).The as-prepared MgH_(2)-H-V_(2)O_(5) composites exhibit low desorption temperatures(Tonset=185℃)with a hydrogen capacity of 6.54 wt%,fast kinetics(Ea=84.55±1.37 kJ mol^(-1) H_(2) for desorption),and long cycling stability.Impressively,hydrogen absorption can be achieved at a temperature as low as 30℃ with a capacity of 2.38 wt%within 60 min.Moreover,the composites maintain a capacity retention rate of~99%after 100 cycles at 275℃.Experimental studies and theoretical calculations demonstrate that the in-situ formed VH_(2)/V catalysts,unique 2D structure of H-V_(2)O_(5) nanosheets,and abundant oxygen vacancies positively contribute to the improved hydrogen sorption properties.Notably,the existence of oxygen vacancies plays a double role,which could not only directly accelerate the hydrogen ab/de-sorption rate of MgH_(2),but also indirectly affect the activity of the catalytic phase VH_(2)/V,thereby further boosting the hydrogen storage performance of MgH_(2).This work highlights an oxygen vacancy excited“hydrogen pump”effect of VH_(2)/V on the hydrogen sorption of Mg/MgH_(2).The strategy developed here may pave a new way toward the development of oxygen vacancy-rich transition metal oxides catalyzed hydride systems.

关键词： Hydrogen storage MgH_(2) V_(2)O_(5)nanosheets Oxygen vacancies VH_(2)

Lactobacillus plantarum AR495 improves stress-induced irritable bowel syndrome in rats by targeting gut microbiota and Mast cell-PAR2-TRPV1 signaling pathway

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Food Science and Human Wellness 2024年第2期13卷 698-708页

作者： Hongyun Zhang Guangqiang Wang Zhiqiang Xiong Zhuan Liao Yangyan Qian Xin Song Li Sui Lianzhong Ai Yongjun Xia shanghai engineering research center of Food Microbiology School of Health Science and EngineeringUniversity of Shanghai for Science and TechnologyShanghai 200093China Department of Gastroenterol Digestive Endoscopy CenterShanghai HospitalShanghai 200093China

Probiotics have great potential in regulating intestinal pain.In this study,the effects of Lactobacillus plantarum AR495 on the visceral sensitivity and gut microbiota of irritable bowel syndrome(IBS)rats were studied.The results showed that tryptase released after mast cell activation and degranulation plays a key role in visceral pain,and L.plantarum AR495 reduced the stimulation of colonic mast cells and the expression of protease-activated receptor 2(PAR2)and TRPV1 in dorsal root ganglia.research further showed that supplementation with L.plantarum AR495 increased the level of short-chain fatty acids(SCFAs)and enhanced the barrier function of the colon.In addition,the microbiota analysis of the colon indicated that L.plantarum AR495 promoted the proliferation of Bifidobacterium and inhibited the proliferation of Lachnospiraceae,which alleviated the imbalance of the intestinal microbiota caused by IBS to a certain extent.In total,L.plantarum AR495 might reduce visceral sensitivity through the Mast cell-PAR2-TRPV1 signaling pathway by maintaining the homeostasis of the intestinal barrier.

关键词： Lactobacillus plantarum Irritable bowel syndrome Visceral sensitivity Dorsal root ganglia Tryptase Microbiota

Promoted catalytic performance of Ag-Mn bimetal catalysts synthesized through reduction route

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Journal of Environmental Sciences 2024年第3期137卷 358-369页

作者： Wenkai Hu Tao Guo Kaiyao Ma Xu Li Wangting Luo Mingzhi Wu Hao Guo Yaxin Zhang Wenfeng Shangguan School of Chemical engineering and Technology Xinjiang UniversityUrumchi 830017China research center for Combustion and Environmental Technology Shanghai Jiao Tong UniversityShanghai 200240China

VOCs can exert great harm to both human and environment,and catalytic oxidation is believed to be an effective technique to eliminate these pollutants.In this paper,Ag-Mn bimetal catalysts with 10 wt.%of silver were synthesized using doping,impregnation,and reduction methods respectively,and then they were applied to the catalytic oxidation of benzene.Through series of characterizations it showed that the loading of silver using reduction method significantly resulted in improved physico-chemical properties of manganese oxides,such as larger surface area and pore volume,higher proportion of surface Mn~(3+)and Mn~(4+),stronger reducibility and more active of surface oxygen species,which were all beneficial to its catalytic activity.As a result,the Ag-Mn catalysts synthesized by reduction method showed a lower T_(90)value(equals to the temperature at which 90%of initial benzene was removed)of 203℃.Besides,both the used and fresh Ag-Mn catalysts synthesized by reduction method showed preferable stability in this research.

关键词： Ag-Mn Benzene oxidation Catalytic performance Reduction route

Buckling Properties of Water-Drop-Shaped Pressure Hulls with Various Shape Indices Under Hydrostatic External Pressure

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China Ocean engineering 2024年第1期38卷 1-17页

作者： DING Xiao-de ZHANG Jian WANG Fang JIAO Hui-feng WANG Ming-lu School of Mechanical engineering Jiangsu University of Science and TechnologyZhenjiang 212003China shanghai engineering research center of Marine Renewable Energy College of Engineering Science and Technol-ogyShanghai Ocean UniversityShanghai 201306China China Ship Scientific research center Wuxi 214082China

The water-drop-shaped pressure hull has a good streamline,which has good application prospect in the underwater observatory.Therefore,this study conducted analytical,experimental and numerical investigation of the buckling properties of water-drop-shaped pressure hulls under hydrostatic pressure.A water-drop experiment was conducted to design water-drop-shaped pressure hulls with various shape indices.The critical loads for the water-drop-shaped pressure hulls were resolved by using Mushtari’s formula.Several numerical simulations including linear buckling analysis and nonlinear buckling analysis including eigenmode imperfections were performed.The results indicated that the critical loads resolved by Mushtari's formula were in good agreement with the linear buckling loads from the numerical simulations.This formula can be extended to estimate the buckling capacity of water-drop-shaped pressure hulls.In addition,three groups of pressure hulls were fabricated by using stereolithography,a rapid prototyping technique.Subsequently,three groups of the pressure hulls were subjected to ultrasonic measurements,optical scanning,hydrostatic testing and numerical analysis.The experimental results were consistent with the numerical results.The results indicate that the sharp end of the water-drop-shaped pressure hulls exhibited instability compared with the blunt end.This paper provides a new solution to the limitations of experimental studies on the water-drop-shaped pressure hulls as well as a new configuration and evaluation method for underwater observatories.

关键词： buckling pressure hull water drop underwater observatory

A Comparison of Four Neural Networks Algorithms on Locomotion Intention Recognition of Lower Limb Exoskeleton Based on Multi-source Information

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Journal of Bionic engineering 2024年第1期21卷 224-235页

作者： Duojin Wang Xiaoping Gu Hongliu Yu Institute of Rehabilitation engineering and Technology University of Shanghai for Science and Technology516 Jungong RoadShanghai 200093China shanghai engineering research center of Assistive Devices 516 Jungong RoadShanghai 200093China

Lower Limb Exoskeletons(LLEs)are receiving increasing attention for supporting activities of daily living.In such active systems,an intelligent controller may be indispensable.In this paper,we proposed a locomotion intention recognition system based on time series data sets derived from human motion signals.Composed of input data and Deep Learning(DL)algorithms,this framework enables the detection and prediction of users’movement patterns.This makes it possible to predict the detection of locomotion modes,allowing the LLEs to provide smooth and seamless assistance.The pre-processed eight subjects were used as input to classify four scenes:Standing/Walking on Level Ground(S/WOLG),Up the Stairs(US),Down the Stairs(DS),and Walking on Grass(WOG).The result showed that the ResNet performed optimally compared to four algorithms(CNN,CNN-LSTM,ResNet,and ResNet-Att)with an approximate evaluation indicator of 100%.It is expected that the proposed locomotion intention system will significantly improve the safety and the effectiveness of LLE due to its high accuracy and predictive performance.

关键词： Lower limb exoskeletons(LLEs) Locomotion intention ResNet Multi-source

Transfer of disulfide bond formation modules via yeast artificial chromosomes promotes the expression of heterologous proteins inKluyveromyces marxianus

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mLife 2024年第1期3卷 129-142页

作者： Pingping Wu Wenjuan Mo Tian Tian Kunfeng Song Yilin Lyu Haiyan Ren Jungang Zhou Yao Yu Hong Lu State Key Laboratory of Genetic engineering School of Life SciencesFudan UniversityShanghaiChina shanghai engineering research center of Industrial Microorganisms ShanghaiChina

Kluyveromyces marxianus is a food-safe yeast with great potential for producing heterologous proteins.Improving the yield in K.marxianus remains a challenge and incorporating large-scale functional modules poses a technical obstacle in engineering.To address these issues,linear and circular yeast artificial chromosomes of K.marxianus(KmYACs)were constructed and loaded with disulfide bond formation modules from Pichia pastoris or K.marxianus.These modules contained up to seven genes with a maximum size of 15 kb.KmYACs carried telomeres either from K.marxianus or Tetrahymena.KmYACs were transferred successfully into K.marxianus and stably propagated without affecting the normal growth of the host,regardless of the type of telomeres and configurations of KmYACs.KmYACs increased the overall expression levels of disulfide bond formation genes and significantly enhanced the yield of various heterologous proteins.In high-density fermentation,the use of KmYACs resulted in a glucoamylase yield of 16.8 g/l,the highest reported level to date in K.marxianus.Transcriptomic and metabolomic analysis of cells containing KmYACs suggested increased flavin adenine dinucleotide biosynthesis,enhanced flux entering the tricarboxylic acid cycle,and a preferred demand for lysine and arginine as features of cells overexpressing heterologous proteins.Consistently,supplementing lysine or arginine further improved the yield.Therefore,KmYAC provides a powerful platform for manipulating large modules with enormous potential for industrial applications and fundamental research.Transferring the disulfide bond formation module via YACs proves to be an efficient strategy for improving the yield of heterologous proteins,and this strategy may be applied to optimize other microbial cell factories.

关键词： disulfide bond formation expression of heterologous proteins Kluyveromyces marxianus telomere yeast artificial chromosome

A multifunctional electrolyte additive for zinc-ion capacitors with low temperature resistant and long lifespan

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Journal of Energy Chemistry 2024年第7期94卷 477-485页

作者： Ming Sun Zekai Zhang Shuqing Fu Yifan Zhang Ruoyu Wang Hongchun Mu Cheng Lian Wenqiang Wang Gengchao Wang shanghai engineering research center of Hierarchical Nanomaterials Shanghai Key Laboratory of Advanced Polymeric MaterialsSchool of Materials Science and EngineeringEast China University of Science and TechnologyShanghai 200237China State Key Laboratory of Chemical engineering Shanghai Engineering Research Center of Hierarchical NanomaterialsFrontiers Science Center for Materiobiology and Dynamic Chemistryand School of Chemistry and Molecular EngineeringEast China University of Science and TechnologyShanghai 200237China State Key Laboratory of Molecular engineering of Polymers Department of Macromolecular ScienceFudan UniversityShanghai 200438China

Aqueous zinc-ion capacitors(ziCs)are considered as potential candidates for next generation electro-chemical energy storage devices due to their high safety and low cost.However,the existing aqueous ZiCs usually have the problems of zinc dendrite growth and unsatisfactory performance at low temper-ature.Herein,an erythritol(Eryt)additive with inhibition of zinc dendrites and anti-freezing capability was introduced into the ZnSO_(4) electrolyte.The experimental characterization and theoretical calculation confirm that the Eryt adsorbed on the surface of zinc anodes regulates the deposition orientation of Zn^(2+)and inhibits the formation of dendrites.It also reconstructs the solvation structure in the electrolyte to reduce water activity,enabling the electrolyte to have a lower freezing point for operation at low tem-perature.With the assistance of Eryt,the ZnZn symmetric cell exhibits a long cycle life of 2000 h,while the ZIC assembled with activated carbon(AC)cathode and zinc anode(ZnllAC)maintains a capacity retention of 98.2%after 30,000 cycles at a current density of 10 A g^(-1)(even after 10,000 cycles at-20°C,the capacity retention rate reached 94.8%.).This work provides a highly scalable,low-cost and effective strategy for the protection of the anodes of low-temperature aqueous ZiCs.

关键词： Zinc-ioncapacitors Electrolyte additive Dendrite-free Freeze-resistant electrolyte

TCERG1L hypermethylation is a risk factor of diabetic retinopathy in Chinese children with type 1 diabetes

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International Journal of Ophthalmology(English edition) 2024年第3期17卷 537-544页

作者： Yu Qian Ying Xiao Qiu-Rong Lin Zhao-Yu Xiang Li-Pu Cui Jia-Qi Sun Si-Cong Li Xin-Ran Qin Hai-Dong Zou Chen-Hao Yang Pei-Yao Jin Department of Ophthalmology Shanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai First People’s HospitalShanghai 200080China shanghai Eye Diseases Prevention&Treatment center Shanghai Eye HospitalShanghai 200040China National Clinical research center for Eye Diseases Shanghai Clinical Research Center for Eye DiseasesShanghai Key Clinical SpecialtyShanghai Key Laboratory of Ocular Fundus DiseasesShanghai Engineering Center for Visual Science and PhotomedicineShanghai Engineering Center for Precise Diagnosis and Treatment of Eye DiseasesShanghai 200080China Department of Ophthalmology Children’s Hospital of Fudan UniversityShanghai 201102China

●AIM:To identify the differential methylation sites(DMS)and their according genes associated with diabetic retinopathy(DR)development in type 1 diabetes(T1DM)children.●METHODS:This study consists of two surveys.A total of 40 T1DM children was included in the first survey.Because no participant has DR,retina thinning was used as a surrogate indicator for DR.The lowest 25%participants with the thinnest macular retinal thickness were included into the case group,and the others were controls.The DNA methylation status was assessed by the Illumina methylation 850K array BeadChip assay,and compared between the case and control groups.Four DMS with a potential role in diabetes were identified.The second survey included 27 T1DM children,among which four had DR.The methylation patterns of the four DMS identified by 850K were compared between participants with and without DR by pyrosequencing.●RESULTS:In the first survey,the 850K array revealed 751 sites significantly and differentially methylated in the case group comparing with the controls(|Δβ|>0.1 and Adj.P0.05)between participants with and without DR.●CONCLUSION:The hypermethylation of the TCERG1L gene is a risk factor for DR development in Chinese children with T1DM.

关键词： DNA methylation 850K array pyrosequencing diabetic retinopathy type 1 diabetes children