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Effects ofβ-1,3-glucan and ascorbic acid on the nutritional-immune response and antioxidant signaling pathways of live tiger grouper during simulated transport

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Aquaculture and Fisheries 2024年第2期9卷 265-272页

作者： Bo Wu Zhenkun Xu Jie Cao Qi Wang Jun Mei Jing Xie College of Food Science and Technology Shanghai Ocean UniversityShanghai201306China National Experimental Teaching Demonstration center for Food Science and engineering Shanghai Ocean UniversityShanghai201306China shanghai engineering research center of aquatic product processing and preservation Shanghai201306China shanghai Professional Technology Service Platform on Cold Chain Equipment Performance and Energy Saving Evaluation Shanghai201306China

Transport in water is the most common method for transporting live fish in China,however,transport is a strong *** stress could lead to a reduced immune and antioxidant system function of tiger grouper,resulting in sickness and ***,tiger grouper were continuously stressed during transport,which resulted in quality *** is necessary that find a way to relieve the stress of transportation of tiger *** acid is not only a good anti-stress agent,but it is also an effective immunostimulant.β-1,3-glucan is a feed additive that can enhance the immune response of ***,this study evaluated the effects ofβ-1,3-glucan and ascorbic acid on the nutritional-immune response and antioxidant signaling pathways of live tiger grouper during simulated *** indicated that addingβ-1,3-glucan and ascorbic acid in transport-water muted the increase of alanine aminotransferase(ALT)and aspartate aminotransferase(AST)*** addition,β-1,3-glucan and ascorbic acid activated Nrf2 and mediated TOR expression and then up-regulate related mRNA expression of antioxidant and immune *** concluded that the application ofβ-1,3-glucan and ascorbic acid inhibit the increase of metabolism enzymes and inflammatory factors and activate immune and antioxidant signaling pathways to relieve oxidant stress,immune response,and *** the loss of amino acids provided nutrients to relieve oxidative stress and immune response,which demonstrated immune-nutritional response in live tiger grouper during simulated *** results may provide a new solution for alleviating the decline of immune and antioxidant function of tiger grouper caused by transportation stress.

关键词： Tiger grouper β-1,3-Glucan Ascorbic acid Immune response Antioxidant signaling pathway

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Untargeted metabolomic profiling， nutritional composition and enzyme changes on Bigeye tuna(Thunnus obesus) during cold storage by HPLC-MS coupled with logistic regression

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Food Science and Human Wellness 2024年

作者： Xin-Yun Wang Zi-Xuan Zhao Jun Yan Jing Xie shanghai engineering research center of aquatic product processing & preservation shanghai Ocean University The International Peace Maternity and Child Health Hospital School of Medicine shanghai Jiao Tong University National Experimental Teaching Demonstration center for Food Science and engineering shanghai Ocean University College of Food Science & Technology shanghai Ocean University

Bigeye tuna is a protein-rich fish that is susceptible to spoilage during cold storage， however， there is limited information on untargeted metabolomic profiling of bigeye tuna concerning spoilage-associated enzymes and metabolites. This study aimed to investigate how cold storage affects enzyme activities， nutrient composition， tissue microstructures and spoilage metabolites of bigeye tuna. The activities of cathepsin B， H， L increased， while Na+/K+-ATPase and Mg2＋-ATPase decreased， α-glucosidase （AG） and β-1，4-N-acetylglucosaminidase （NAG）， lipase and lipoxygenase first increased and then decreased during cold storage， suggesting that proteins undergo degradation and ATP metabolism occurs at a faster rate during cold storage. Nutrient composition （moisture， protein， and lipid content）， free amino acids， total amino acids decreased， suggesting that the nutritional value of bigeye tuna was reduced. Besides， a logistic regression equation has been established as a food analysis tool and assess the dynamics and correlation of the enzyme of bigeye tuna during cold storage. Based on untargeted metabolomic profiling analysis， a total of 484 metabolites were identified as significantly differential metabolites in the bigeye tuna contained several spoilage metabolites involved in lipid metabolism （1-Acyl-sn-glycero-3phosphocholine， Glycerophosphocholine， Choline phosphate）， amino acid metabolism (L-Histidine， 5-Deoxy-5'-（methylthio）adenosine， 5-Methylthioadenosine)， carbohydrate metabolism （D-Gluconicacid， beta-D-Fructose 1，6-bisphosphate， D-Glyceraldehyde 3-phosphate）. The results of tissue microstructures of tuna showed a looser network and visible deterioration of tissue fiber during cold storage. Therefore， metabolomic analysis and tissue microstructures provide insight into the spoilage mechanism investigations on bigeye tuna during cold storage.

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Optimization of product Distribution for MIP Units Using Data Mining

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China Petroleum processing & Petrochemical Technology 2024年第2期26卷 146-157页

作者： Wang Qing Zhang Xiaoguo Mei Junwei Gao Zhibo Yang Kuizhi Yang Dawei Ouyang Fusheng International Joint research center of Green Energy Chemical engineering East China University of Science and TechnologyShanghai 200237China SINOPEC Changling Petrochemical Co. LtdYueyang 414012China

Based on data from a petrochemical company’s MIP unit over the past three years,19 input variables and 2 output variables were selected for modeling using the maximum information coefficient and Pearson correlation coefficient among 155 variables,which included properties of feedstock oil and spent catalyst,operational variables,and material *** distillation range variables were reduced using factor analysis,and the feedstock oils were clustered into three types using the K-means++*** feedstock oil type was then used as an input variable for *** XGBoost model and a back propagation(BP)neural network model with a structure of 20-15-15-2 were developed to predict the combined yield of gasoline and propylene,as well as the coke *** the test set,the BP neural network model demonstrated better fitting and generalization abilities with a mean absolute percentage error and determination coefficient of 1.48%and 0.738,respectively,compared to the XGBoost *** was therefore chosen for further optimization *** genetic algorithm was utilized to optimize operational variables in order to increase the combined yield of gasoline and propylene while controlling the growth of coke *** commercial test results in the MIP unit showed an average increase of 1.39 percentage points for the combined yield of gasoline and propylene and an average decrease of 0.11 percentage points for coke *** results indicate that the model effectively improves the combined yield of gasoline and propylene while controlling the increase in coke yield.

关键词： MIP process K-Means++ BP neural network model XGBoost algorithm genetic algorithm

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Preparation of multicore millimeter-sized spherical alginate capsules to specifically and sustainedly release fish oil

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Food Science and Human Wellness 2023年第2期12卷 397-406页

作者： Lina Tao Panpan Wang Ting Zhang Mengzhen Ding Lijie Liu Ningping Tao Xichang Wang Jian Zhong National R&D Branch center for Freshwater aquatic products processing Technology(shanghai) Integrated Scientific Research Base on Comprehensive Utilization Technology for By-Products of Aquatic Product ProcessingMinistry of Agriculture and Rural Affairs of the People's Republic of ChinaShanghai Engineering Research Center of Aquatic-Product Processing and PreservationCollege of Food Science&TechnologyShanghai Ocean UniversityShanghai 201306China

Specific and sustained release of nutrients from capsules to the gastrointestinal tract has attracted many attentions in the field of food and drug *** this work,we reported a monoaxial dispersion electrospraying-ionotropic gelation technique to prepare multicore millimeter-sized spherical capsules for specific and sustained release of fish *** spherical capsules had diameters from 2.05 mm to 0.35 mm with the increased applied *** capsules consisted of uniform(at applied voltages of≤10 k V)or nonuniform(at applied voltages of>10 k V)*** obtained capsules had reasonable loading ratios(9.7%-6.3%)due to the multicore *** addition,the obtained capsules had specific and sustained release behaviors of fish oil into the small intestinal phase of in vitro gastrointestinal tract and small intestinal tract *** simple monoaxial dispersion electrospraying-ionotropic gelatin technique does not involve complicated preparation formulations and polymer modification,which makes the technique has a potential application prospect for the fish oil preparations and the encapsulation of functional active substances in the field of food and drug industries.

关键词： Ionotropic gelation Millimeter-sized spherical capsule Monoaxial dispersion electrospraying Multicore Specific and sustained release

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Chemical characterization of a new sulfated polysaccharide from Gracilaria chouae and its activation effects on RAW264.7 macrophages

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2022年第1期23卷 84-88页

作者： Feifei LI Kehai LIU Kewu LIU College of Food Science and Technology Shanghai Ocean UniversityShanghai201306China shanghai engineering research center of aquatic-product processing&preservation Shanghai201306China Heilongjiang Forest By-product and Speciality Institute Mudanjiang157011China

This study aimed to characterize the chemical composition of a new sulfated polysaccharide from the red alga Gracilaria chouae and evaluate its activation effects on RAW264.7 *** showed that the obtained *** polysacch... 详细信息

关键词： activation RAW264.7 characterization

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Characterization of refined fish oil from small fish in Mauritania

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Aquaculture and Fisheries 2022年第6期7卷 639-646页

作者： Xuebing Zhang Liu Lin Zhuanxia Chen Jing Zhang Xichang Wang Ningping Tao College of Food Science and Technology Shanghai Ocean UniversityShanghai201306China shanghai engineering research center of aquatic-product processing&preservation Shanghai201306China National R&D Branch center for Freshwater aquatic products processing Technology Shanghai201306China

In this study,the effects of chemical refining on the physicochemical indices,environmental pollutants,lipid functional groups,relaxation characteristics,fatty acid profiles,and triglyceride(TG)molecular species in crude oil extracted from small fish in Mauritania were *** acid,peroxide,and iodine values of crude oil were identified as 5.52 mg KOH/g,1.73 meq/kg,and 200.78 g/100 g oil,*** refining,these values were determined to be 0.29 mg KOH/g,0.76 meq/kg,and 210.80 g/100 g oil,*** polychlorinated biphenyls and arsenic content of crude oil were identified as 32.30μg/kg and 1.00 mg/kg,*** refining,these were not *** content of eicosapentaenoic acid(EPA)and docosahexaenoic acid(DHA)accounted for as much as 87.55%of the polyunsaturated fatty acids in the refined oil,indicating that high-quality fish oil is suitable as a food *** correlation coefficient of the infrared spectra before and after refining was 0.968,and the relaxation time and peak shape data were almost similar before and after ***,there were no significant differences(P≥0.05)in fatty acid profiles between the crude and refined oils,except for C16:0(P<0.01).Based on UPLC-Q E-MS/MS detection,137 TG molecules were identified in both crude and refined *** the detected TG molecular species,64 contained EPA or DHA,which accounted for 46.83%and 46.00%of the total TG content in crude and refined oils,*** refining,the content of the molecular species decreased by 31.95 mg/10 g,among which the three most variable molecular species were TG(16:0/18:1/22:6),(16:1/16:2/18:1),and(18:4/16:0/20:5),accounting for 29.26%of the decline of the molecular ***,the refining process had little effect on the fatty acid profile and distribution of TG molecular species in fish oil.

关键词： ω-3 PUFAs Crude fish oil Fish oil refinement Triglyceride molecular species Mauritania Sea Small pelagic fish

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Metabolomics mechanism of traditional soy sauce associated with fermentation time

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Food Science and Human Wellness 2022年第2期11卷 297-304页

作者： Li Zhu Siyu He Ying Lu Jianhong Gan Ningping Tao Xichang Wang Zaoli Jiang Yuanxiang Hong Changhua Xu College of Food Science and Technology Shanghai Ocean UniversityShanghai 201306China shanghai engineering research center of aquatic-product processing&preservation Shanghai 201306China Laboratory of Quality and Safety Risk Assessment for aquatic products on Storage and preservation(shanghai) Ministry of AgricultureShanghai 201306China Department of Pharmacology Yale UniversityNew HavenCT06520US Xiamen Gulong Food Co. Ltd.Xiamen 361000China National R&D Branch center for Freshwater aquatic products processing Technology(shanghai) Shanghai 201306China

Given that fermentation time has significant impact on quality and flavor of soy sauce,a global understanding of the metabolic mechanism as fermentation time prolonged is essential for producing satisfactory and consistent quality of traditional soy ***,the metabolic compounds changes of soy sauce associated with fermentation time up to 10 years were comprehensively investigated by using Chinese traditional fermented soy sauce(CTFSS)as a *** showed that formaldehyde nitrogen,total soluble nitrogen(TSN),non-salt soluble solids,amino acids,free 5’-nucleotides and volatile compounds in CTFSS changed obviously during ***,glutamic acid and aspartic acid were prominent in *** decrease in content of hypoxanthine(Hx)was found from 1 M(1-month soy sauce)to7 M(7-months soy sauce).Furthermore,a significant opposite tendency for changes between some volatile compounds and amino acids was indicated that there was a transformation between these two ***,a better understanding to the influence of fermentation time on soy sauce had been *** the formation and conversion of amino acids and sugars might be mainly responsible for flavor formation in CTFSS,the ratio of these two reactions rate led the metabolism to be divided into three steps,degradation,conversion and balance.

关键词： Soy sauce Fermentation Amino acids Volatile compounds Flavor Metabolomics

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The application of Melissa officinalis *** oil nanoemulsions protects sea bass(Lateolabrax japonicus)against myofibrillar protein and lipid oxidation during refrigeration

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Food Quality and Safety 2023年第3期7卷 447-455页

作者： Yao Xie Huijie Yu Xin Zhao Chuhan Bian Hao Cheng Jun Mei Jing Xie College of Food Science&Technology Shanghai Ocean UniversityShanghaiChina shanghai Professional Technology Service Platform on Cold Chain Equipment Performance and Energy Saving Evaluation Shanghai Ocean UniversityShanghaiChina National Experimental Teaching Demonstration center for Food Science and engineering Shanghai Ocean UniversityShanghaiChina shanghai engineering research center of aquatic product processing&preservation Shanghai Ocean UniversityShanghaiChina

Objectives:The nutrient rich sea bass is prone to oxidation of lipid and protein during *** and Methods:The research was to investigate the effect of different concentrations of Melissa officinalis *** oil(MOEO)nanoemulsions on myofibrillar protein(MP)and lipid oxidation in sea bass(Lateolabrax japonicus)during refrigeration at 4°***:The results of thiobarbituric acid reactive substances and mitochondrial membrane potential showed that carboxymethyl chitosan/locust bean gum active coating solutions incorporating 2%MOEO nanoemulsions(C/L-2M)was the most effective in inhibiting lipid oxidation that occurred in sea bass under attack by reactive oxygen ***-field nuclear magnetic resonance results showed that C/L-2M maximally slowed the conversion of bound water to free water during *** oxidation of lipids and MP disrupted the secondary and tertiary conformations of MP and accelerated protein aggregation and ***:C/L-2M slowed the oxidation of lipids and proteins by inhibiting the oxidation of reactive oxygen species.C/L-2M is a very promising preservative emulsion for the preservation of sea bass.

关键词： Lateolabrax japonicus Melissa officinalis L.essential oil lipid oxidation protein oxidation active coating solutions

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Ultra-thin CoAl layered double hydroxide nanosheets for the construction of highly sensitive and selective QCM humidity sensor

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中国化学快报:英文版 2023年第8期34卷 376-381页

作者： Yongheng Zhu Xuhua Dong Jinsheng Cheng Lumin Wang Cheng Zhao Yonghui Deng Siqi Xie Yingjie Pan Yong Zhao Gengzhi Sun Tianjun Ni College of Food Science and Technology Laboratory of Quality&Safety Risk Assessment for Aquatic Products on Storage and Preservation(Shanghai)Ministry of Agriculture and Shanghai Engineering Research Center of Aquatic-Product Processing&Preservation Shanghai Ocean UniversityShanghai 201306China School of Basic Medicine Xinxiang Medical UniversityXinxiang 453003China Key Laboratory of Flexible Electronics(KLOFE)&Institute of Advanced Materials(IAM) Jiangsu National Synergetic Innovation Center for Advanced Materials(SICAM)Nanjing Tech University(NanjingTech)Nanjing 211816China Department of Chemistry FudanUniversityShanghai 200433China Henry-Fork School of Food Sciences Shaoguan UniversityShaoguan 512005China

To achieve real-time monitoring of humidity in various applications,we prepared facile and ultra-thin CoAl layered double hydroxide(CoAl LDH)nanosheets to engineer quartz crystal microbalances(QCM).The characteristics of CoAl LDH were investigated by transmission electron microscopy(TEM),X-ray diffraction(XRD),X-ray photoelectric spectroscopy(XPS),Brunauer–Emmett–Telle(BET),atomic force microscopy(AFM)and zeta *** to their large specific surface area and abundant hydroxyl groups,CoAl LDH nanosheets exhibit good humidity sensing *** a range of 11.3%and 97.6%relative humidity(RH),the sensor behaved an ultrahigh sensitivity(127.8 Hz/%RH),fast response(9.1 s)and recovery time(3.1 s),low hysteresis(3.1%RH),good linearity(R^(2)=0.9993),stability and ***,the sensor can recover the initial response frequency after being wetted by deionized water,revealing superior self-recovery ability under high *** on in-situ Fourier transform infrared spectroscopy(FT-IR),the adsorption mechanism of CoAl LDH toward water molecules was *** QCM sensor can distinguish different respiratory states of people and wetting degree of fingers,as well as monitor the humidity in vegetable packaging,suggesting excellent properties and a promising application in humidity sensing.

关键词： Layered double hydroxide nanosheets Quartz crystal microbalance Humidity sensor Respiratory monitoring Sensing mechanism

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The preservation effects of chitosan copolymers(gallic acid and protocatechuic acid)on sea bass(Lateolabrax japonicus)fillets

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Aquaculture and Fisheries 2023年第3期8卷 305-315页

作者： Jiali Liu Weiqing Lan Yi Wu Xiaohong Sun Jun Mei Yiheng Chen Jing Xie College of Food Science and Technology Shanghai Ocean UniversityShanghai201306China shanghai aquatic products processing and Storage engineering Technology research center Shanghai201306China National Experimental Teaching Demonstration center for Food Science and engineering(shanghai Ocean University) Shanghai201306China Laboratory of Quality&Safety Risk Assessment for aquatic products on Storage and preservation(shanghai) Ministry of AgricultureShanghai201306China

This study was aimed at preparing chitosan(CS)-phenolic acids copolymers to obtain better preservation effect in marine *** this work,CS was grafted onto phenolic acids such as gallic acid(GA)and protocatechuic acid(PA)by a carbodiimide coupling *** copolymers(CS-grafted-GA(CS-g-GA)and CS-grafted-PA(CS-g-PA))were further confirmed through physicochemical properties including ultraviolet-visible(UV-Vis)spectrophotography,fourier transform infrared(FTIR)spectrometry,differential scanning calorimetry(DSC)thermogram,rheological behaviour,scanning electron microscope(SEM)and antioxidant activity ***,the effects of copolymers on preservation of refrigerated sea bass(Lateolabrax japonicus)fillets were *** results showed that the CS-g-GA(110.82 mg GA/g)exhibited higher grafting rate than CS-g-PA(62.63 mg PA/g).According to the assessment of UV-Vis and FTIR spectrometry,both the GA and PA were grafted onto CS *** analysis demonstrated that the microstructure of copolymers became ***,both the CS-g-GA and CS-g-PA copolymers displayed superior thermal stability,rheological properties and antioxidant capacity than the pure ***,the copolymers also revealed a significantly stronger preservation effect on sea bass fillets including inhibiting microbial growth and delaying the deterioration of color,texture and sensory ***,CS-g-GA and CS-g-PA could be used as promising preservatives for seafood.

关键词： Sea bass fillets Chitosan Grafted Coating Carbodiimide coupling Antioxidant capacity

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