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Production and characterization of monoclonal antibodies against grass carp CD4-1 and CD4-2

Aquaculture and Fisheries 2024年第2期9卷 154-163页

作者： Bangjie Wang Yuting Qin Zhaosheng Sun Xiaozhen Zhu Zhao Jia Kangyong Chen Danjie Liu Junya Wang Jun Zou Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Ministry of EducationShanghai Ocean UniversityShanghai201306China International Research Center for Marine Biosciences at shanghai Ocean University Ministry of Science and TechnologyShanghai201306China National Demonstration Center for Experimental Fisheries Science education Shanghai Ocean UniversityShanghai201306China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao266071China

CD4 T helper cells are an important group of cells in the immune system of vertebrates and express CD4 receptor on the cell *** mammals,the CD4 receptor is encoded by a single copy gene,whilst in fish,two copies of cd4 genes,namely cd4-1 and cd4-2,are *** this study,the ectodomains of grass carp(Ctenopharyngodon idella,Ci)CD4-1 and CD4-2 were expressed in the *** cells and used to generate monoclonal antibodies in *** blotting,confocal microscopy and flow cytometry were performed to characterize the monoclonal *** has been shown that the CiCD4-1 and CiCD4-2 monoclonal antibodies had good specificity to react with the recombinant ectodomains of CiCD4-1 and CiCD4-2 expressed in the CHO-S cells and the native CD4 molecules of grass *** CiCD4-1 monoclonal antibody did not recognize CiCD4-2 and verse *** addition,the CD4-1 and CD4-2 monoclonal antibodies specifically recognized the CD4-1 and CD4-2 receptors expressed in the HEK293 cells and native molecules of fish ***,the percentages of lymphocytes in immune tissues of healthy fish were analyzed by flow *** was found that 17.6%of lymphocytes were CD4-1^(+)cells and 22.5%were CD4-2^(+)in the head *** the spleen,13.1%of lymphocytes were CD4-1^(+)and 18.6%were CD4-2^(+)while 7.3%of blood lymphocytes were CD4-1^(+)cells and 8.8%were CD4-2^(+)*** availability of the CD4-1 and CD4-2 monoclonal antibodies provides antibody-based tools for further elucidation of the functions of CD4 T cells in grass carp.

关键词： Grass carp CD4 Monoclonal antibodies Flow cytometry Confocal microscopy

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Exploring the feasibility of prestressed anchor cables as an alternative to temporary support in the excavation of super-large-span tunnel

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Railway Engineering Science 2024年第3期32卷 344-360页

作者： Shunhua Zhou Yuyin Jin Zhiyao Tian Chunhua Zou Heming Zhao Zengrun Miao shanghai Key Laboratory of Rail Infrastructure Durability and System Safety Tongji UniversityShanghai 201804China Key Laboratory of Road and Traffic Engineering of the Ministry of education Tongji UniversityShanghai 201804China China Railway Construction Corporation Limited Beijing 100855China The 2nd Engineering Co. Ltd.of China Railway Construction Bridge Engineering Bureau GroupShenzhen 518083China China Railway Construction Bridge Engineering Bureau group Co. LtdTianjin 300300China

Excavating super-large-span tunnels in soft rock masses presents significant *** ensure safety,the sequential excavation method is commonly *** utilizes internal temporary supports to spatially partition the tunnel face and divide the excavation into multiple ***,these internal supports generally impose spatial constraints,limiting the use of large-scale excavation equipment and reducing construction *** address this constraint,this study adopts the“Shed-frame”principle to explore the feasibility of an innovative support system,which aims to replace internal supports with prestressed anchor cables and thus provide a more spacious working space with fewer internal *** evaluate its effectiveness,a field case involving the excavation of a 24-m span tunnel in soft rock is presented,and an analysis of extensive field data is conducted to study the deformation characteristics of the surrounding rock and the mechanical behavior of the support *** results revealed that prestressed anchor cables integrated the initial support with the shed,creating an effective“shed-frame”system,which successively maintained tunnel deformation and frame stress levels within safe regulatory ***,the prestressed anchor cables bolstered the surrounding rock effectively and reduced the excavation-induced disturbance zone *** summary,the proposed support system balances construction efficiency and *** field experiences may offer valuable insights into the popularization and further development of prestressed anchor cable support systems.

关键词： Super-large-span tunnel Construction safety Sequential excavation method Shed-frame principle Prestressed anchor cables

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Multiple factors influencing high-purity indium electrolytic refining

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Chinese Journal of Chemical Engineering 2024年第7期71卷 148-160页

作者： Hong-Qiang Fan Fei Li Hong-Xing Zheng Wu-ji Pan Mei-Zhen Wu Yashar Behnamian Ju-Bo Peng Dong-Hai Lin State Key Laboratory of Advanced Special Steel&School of Materials Science and Engineering Shanghai UniversityShanghai 200444China shanghai Engineering Research Center for Integrated Circuits and Advanced Display Materials Shanghai UniversityShanghai 200444China Research&Development Center Yunnan Tin Group(Holding)Limited CompanyKunming 650032China Department of Chemical and Materials Engineering University of AlbertaEdmonton T6G 2V4AlbertaCanada School of Energy and Materials Shanghai Key Laboratory of Engineering Materials Application and EvaluationShanghai Polytechnic UniversityShanghai 201209China

The effects of various contaminants in the electrolytic refinement of indium were investigated using a glow discharge mass spectrometer(GDMS).The effects of several factors such as the indium ion(In3+)concentration,the sodium chloride(NaCl)concentration,the current density,the gelatin concentration,the pH,and the electrode distance,were *** variations in impurity levels concerning gelatin concentration were *** the gelatin and In3+concentration were moderately positively correlated with the Pb *** Sb concentration was associated positively with the NaCl concentration,while the Ti concentration had an adverse correlation with the NaCl *** Bi element content was positively linked to the electrode *** the current density increased,Cu,Pb,and Bi impurities initially rose and then eventually ***,a critical current density of 45 A·m^(-2) was identified in this behavior.

关键词： High-purity indium Electrolysis Multiple factors Electrochemistry Purification

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Microfluidic separation of particles by synergistic effect of geometry-induced hydrodynamics and magnetic field

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Chinese Chemical Letters 2024年第2期35卷 380-385页

作者： Du Qiao Hongxia Li Weiping Zhu Lili Zhu Danyang Zhao Honglin Li Key Laboratory for Precision & Non-traditional Machining Technology of Ministry of education Dalian University of TechnologyDalian 116023China shanghai Key Laboratory of New Drug Design School of PharmacyEast China University of Science and TechnologyShanghai 200237China Lingang Lab Shanghai 200031China

Microfluidic combined with magnetic field have been demonstrated to be the promising solutions for fast and low-damage particles ***,the difficulties in the precise layout of magnets and accurate prediction of particle trajectories lead to under and over separation of target particles.A novel particle separation lab-on-chip(LOC)prototype integrated with microstructures and micropolar arrays is designed and ***,a numerical model for the separation of magnetic particles by the synergistic effect of geometry-induced hydrodynamics and magnetic field is *** effect of geometry and magnetic field layout on particle deflection is systematically analyzed to implement accurate prediction of particle *** is found that the separation efficiency of magnetic particles increased from 50.2%to 91.7%and decreased from 88.6%to 85.7%in the range of depth factors from 15µm to 27µm and width factors from 30µm to 60µm,*** particular,the combined effect of the offset distance of permanent magnets and the distance from the main flow channel exhibits a significant difference from the conventional ***,the developed LOC prototype was generalized for extension to arbitrary *** work provides a new insight and robust method for the microfluidic separation of magnetic particles.

关键词： Microfluidic Particle separation Synergistic effect Hydrodynamics Magnetic field Numerical calculation

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Unveiling multi-element synergy in polymetallic oxides for efficient nitrate reduction to ammonia

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Science China Materials 2024年第9期67卷 2941-2948页

作者： Yaning Qie Jiachen Gao Siqi Li Mingjin Cui Xuejiao Mao Xinyu Wang Bo Zhang Sijia Chi Yiran Jia Quan-Hong Yang Chunpeng Yang Zhe Weng Nanoyang group Tianjin Key Laboratory of Advanced Carbon and Electrochemical Energy StorageSchool of Chemical Engineering and Technologyand Collaborative Innovation Center of Chemical Science and Engineering(Tianjin)Tianjin UniversityTianjin300072China National Industry-education Integration Platform of Energy Storage Tianjin UniversityTianjin300072China Haihe Laboratory of Sustainable Chemical Transformations Tianjin300192China Institute of Energy Materials Science University of Shanghai for Science and TechnologyShanghai200093China

Electrocatalytic nitrate reduction reaction is considered as a promising and sustainable method for ammonia ***,the selectivity and yield rate of ammonia are limited by the competitive hydrogen evolution reaction and the complex eight-electron transfer ***,we developed a(FeCoNiCu)Ox/CeO_(2)polymetallic oxide electrocatalyst for effective nitrate reduction to *** synergistic effects among the multiple elements in the electrocatalyst were clearly elucidated by comprehensive ***,Cu acted as the active site for reducing nitrate to nitrite,and Co facilitated the subsequent reduction of nitrite to ammonia,while Fe and Ni promoted water dissociation to provide ***,the incorporation of CeO_(2)increased the active surface area of(FeCoNiCu)Ox,resulting in an improved ammonia yield rate to meet industrial ***,the(FeCoNiCu)Ox/CeO_(2)electrocatalyst achieved an ammonia current density of 382 mA cm^(-2)and a high ammonia yield rate of 30.3 mg h^(-1)cm^(-2)with a long-term *** work offers valuable insights for the future design of highly efficient multi-element electrocatalysts.

关键词： multi-element synergy electrocatalysis ammonia synthesis polymetallic oxides rapid Joule-heating method

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Structural effects of sulfonamides on the proliferation dynamics of sulfonamide resistance genes in the sequencing batch reactors and the mechanism

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Journal of Environmental Sciences 2024年第1期135卷 161-173页

作者： Ligui Wu Mingyu Wang Lingling Rong Wenbiao Wang Linwei Chen Qiaofeng Wu Haoyu Sun Xiangfeng Huang Xiaoming Zou College of Environmental Science and Engineering Tongji UniversityShanghai 200092China School of Life Science Jinggangshan UniversityJi’an 343009China Key Laboratory of Organic Compound Pollution Control Engineering(MOE) School of Environmental and Chemical EngineeringShanghai UniversityShanghai 200444China Fuzhou Urban and Rural Construction group Co.Ltd. Fuzhou 350007China

Antibiotic resistance genes(ARGs)can be easily promoted by antibiotics,however,the structural effects of antibiotics on the proliferation of ARGs dynamic and the associated mechanisms remain obscure in,especially,activated sludge sequencing batch *** the present study,the effects of 9 sulfonamides(SAs)with different structures on the proliferation dynamic of sulfonamide resistance genes(Suls)in the activated sludge sequencing batch reactors and the corresponding mechanisms were determined(30 days),and the results showed that the largest proliferation value(ΔA^(R))of Suls dynamic for SAs(sulfachloropyridazine)was approximately 2.9 times than that of the smallest one(sulfadiazine).The proliferation of Suls was significantly related to the structural features(minHBint6,SssNH,SHBd and SpMax2_Bhm)that represent the biological activity of *** interpret the phenomenon,a mechanistic model was developed and the results indicated that the biodegradation of SAs(T_(1/2))rather than conjugative transfer frequency or mutation frequency tends to be the key process for affecting Suls proliferation.T_(1/2)was proved to be dependent on the interactions between SAs and receptors(E_(binding)),the cleavage mode(bond dissociation energy),and the site of nucleophilic ***,the metagenomic analysis showed that SAs posed significant effect on antibiotic resistome and Tnp31 played a vital role in the proliferation of ***,our findings provide important insight into a theoretical basis for understanding the structural effects of SAs on the proliferation of ARGs in SBR systems.

关键词： Activated sludge Sulfonamides Suls Proliferation Mechanism

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Microstructure and Mechanical Property of the Large Cross-Sectioned Mg-Gd-Y-Zn-Zr Alloy Produced by Small Extrusion Ratio

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Acta Metallurgica Sinica(English Letters) 2024年第6期37卷 999-1006页

作者： Sen Wang Hucheng Pan Caixia Jiang Zhihao Zeng Zhen Pan Weineng Tang Chubin Yang Yuping Ren Gaowu Qin Key Laboratory for Anisotropy and Texture of Materials(Ministry of education) College of Materials Science and EngineeringNortheastern UniversityShenyang110819China Mg and Mg Alloy Institute Technology CenterBaosteel Metal Co.LtdChina Baowu Steel GroupShanghai200940China Jiangxi Provincial Engineering Research Center for Magnesium Alloy Gannan Normal UniversityGanzhou341000China

In this work,the microstructure and mechanical properties of large cross-sectioned Mg-9Gd-3Y-1.2Zn-0.5Zr(VWZ931)samples produced by the small extrusion ratio has been *** as-extruded VWZ931 sample with diameter of~30 mm can exhibit the high yield strength(YS)of 339 MPa,ultimate tensile strength(UTS)of 387 MPa and elongation of 8.2%,*** peak-aged,the YS and UTS of the Mg samples were significantly increased to 435 MPa and 467 *** small extrusion ratio leads to the low fraction of dynamic recrystallized(DRX)grains in VWZ931 sample,and the texture hardening effect can be fully utilized to achieve high *** combined effect of precipitation strengthening due to the long-period stacking ordered phases and theβ′phase,grain boundary strengthening due to the fine DRX grains,heterogeneous deformation-induced strengthening caused by bimodal microstructure,can together contribute to the high strength of present Mg *** findings can shed light on designing other large-sized Mg wrought alloys with high mechanical performance.

关键词： Mg-RE alloy Mechanical property Bimodal grains Texture hardening

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Diverse functions of sox9 in liver development and homeostasis and hepatobiliary diseases

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Genes & Diseases 2024年第4期11卷 170-187页

作者： Taiyu Shang Tianyi Jiang Xiaowen Cui Yufei Pan Xiaofan Feng Liwei Dong Hongyang Wang School of Life Sciences Institute of Metabolism and Integrative BiologyFudan UniversityShanghai 200438China National Center for Liver Cancer The Naval Medical UniversityShanghai 201805China International Cooperation Laboratory on Signal Transduction Eastern Hepatobiliary Surgery InstituteThe Second Military Medical UniversityShanghai 200438China Laboratory of Signaling Regulation and Targeting Therapy of Liver Cancer Second Military Medical University&Ministryof EducationShanghai 200438China

The liver is the central organ for digestion and detoxification and has unique metabolic and regenerative *** hepatobiliary system originates from the foregut endoderm,in which cells undergo multiple events of cell proliferation,migration,and differentiation to form the liver parenchyma and ductal system under the hierarchical regulation of transcription *** on liver development and diseases have revealed that SRY-related high-mobility group box 9(SOx9)plays an important role in liver embryogenesis and the progression of hepatobiliary ***9 is not only a master regulator of cell fate determination and tissue morphogenesis,but also regulates various biological features of cancer,including cancer stemness,invasion,and drug resistance,making Sox9 a potential biomarker for tumor prognosis and *** review systematically summarizes the latest findings of sox9 in hepatobiliary development,homeostasis,and *** also highlight the value of sox9 as a novel biomarker and potential target for the clinical treatment of major liverdiseases.

关键词： Biliary system Cancer Homeostasis Liver diseases SOX9

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Rock mass quality prediction on tunnel faces with incomplete multi-source dataset via tree-augmented naive Bayesian network

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International Journal of Mining Science and Technology 2024年第3期34卷 323-337页

作者： Hongwei Huang Chen Wu Mingliang Zhou Jiayao Chen Tianze Han Le Zhang Key Laboratory of Geotechnical and Underground Engineering of Ministry of education Tongji UniversityShanghai 200092China Department of Geotechnical Engineering Tongji UniversityShanghai 200092China School of Civil Engineering Beijing Jiaotong UniversityBeijing 100044China Qingdao Guoxin Second Jiaozhou Bay Subsea Tunnel Co. Ltd.Qingdao 266071China

Rock mass quality serves as a vital index for predicting the stability and safety status of rock tunnel *** tunneling practice,the rock mass quality is often assessed via a combination of qualitative and quantitative ***,due to the harsh on-site construction conditions,it is rather difficult to obtain some of the evaluation parameters which are essential for the rock mass quality *** this study,a novel improved Swin Transformer is proposed to detect,segment,and quantify rock mass characteristic parameters such as water leakage,fractures,weak *** site experiment results demonstrate that the improved Swin Transformer achieves optimal segmentation results and achieving accuracies of 92%,81%,and 86%for water leakage,fractures,and weak interlayers,respectively.A multisource rock tunnel face characteristic(RTFC)dataset includes 11 parameters for predicting rock mass quality is *** the limitations in predictive performance of incomplete evaluation parameters exist in this dataset,a novel tree-augmented naive Bayesian network(BN)is proposed to address the challenge of the incomplete dataset and achieved a prediction accuracy of 88%.In comparison with other commonly used Machine Learning models the proposed BN-based approach proved an improved performance on predicting the rock mass quality with the incomplete *** utilizing the established BN,a further sensitivity analysis is conducted to quantitatively evaluate the importance of the various parameters,results indicate that the rock strength and fractures parameter exert the most significant influence on rock mass quality.

关键词： Rock mass quality Tunnel faces Incomplete multi-source dataset Improved Swin Transformer Bayesian networks

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Resting-state brain network remodeling after different nerve reconstruction surgeries:a functional magnetic resonance imaging study in brachial plexus injury rats

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Neural Regeneration Research 2025年第5期20卷 1495-1504页

作者： Yunting Xiang Xiangxin Xing Xuyun Hua Yuwen Zhang Xin Xue Jiajia Wu Mouxiong Zheng He Wang Jianguang Xu School of Rehabilitation Science Shanghai University of Traditional Chinese MedicineShanghaiChina Engineering Research Center of Traditional Chinese Medicine Intelligent Rehabilitation Ministry of EducationShanghaiChina Department of Rehabilitation Medicine Yueyang Hospital of Integrated Traditional Chinese and Western MedicineShanghai University of Traditional Chinese MedicineShanghaiChina Department of Traumatology and Orthopedics Yueyang Hospital of Integrated Traditional Chinese and Western MedicineShanghai University of Traditional Chinese MedicineShanghaiChina Institute of Science and Technology for Brain-Inspired Intelligence Fudan UniversityShanghaiChina Key Laboratory of Computational Neuroscience and Brain-Inspired Intelligence(Fudan University) Ministry of EducationShanghaiChina Human Phenome Institute Fudan UniversityShanghaiChina

Distinct brain remodeling has been found after different nerve reconstruction strategies,including motor representation of the affected ***,differences among reconstruction strategies at the brain network level have not been *** study aimed to explore intranetwork changes related to altered peripheral neural pathways after different nerve reconstruction surgeries,including nerve repair,endto-end nerve transfer,and end-to-side nerve ***–Dawley rats underwent complete left brachial plexus transection and were divided into four equal groups of eight:no nerve repair,grafted nerve repair,phrenic nerve end-to-end transfer,and end-to-side transfer with a graft sutured to the anterior upper ***-state brain functional magnetic resonance imaging was obtained 7 months after *** independent component analysis algorithm was utilized to identify group-level network components of interest and extract resting-state functional connectivity values of each voxel within the *** in intra-network resting-state functional connectivity were compared among the *** muscle reinnervation was assessed by behavioral observation(elbow flexion)and *** results showed that alterations in the sensorimotor and interoception networks were mostly related to changes in the peripheral neural *** repair was related to enhanced connectivity within the sensorimotor network,while end-to-side nerve transfer might be more beneficial for restoring control over the affected limb by the original motor *** thalamic-cortical pathway was enhanced within the interoception network after nerve repair and end-to-end nerve *** areas related to cognition and emotion were enhanced after end-to-side nerve *** study revealed important brain networks related to different nerve *** networks may be potential targets for enhancing motor recovery.

关键词： brain functional networks end-to-end nerve transfer end-to-side nerve transfer independent component analysis nerve repair peripheral plexus injury resting-state functional connectivity

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