检索结果-南通市图书馆

Double‑Layer Nanofibrous Sponge Tube via Electrospun Fiber and Yarn for Promoting Urethral Regeneration

Advanced Fiber Materials 2023年第2期5卷 662-680页

作者： Kaile Zhang Muhammad Aqeel Bhutto Liyang Wang Kai Wang Jie Liu Wenyao Li Wenguo Cui Qiang Fu The Department of Urology Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai Jiao Tong UniversityShanghai 200233People’s Republic of China Institute of Biotechnology and Genetic Engineering University of SindhJamshoro 76080Pakistan School of Materials Science and Engineering Shanghai University of Engineering ScienceShanghai 201620People’s Republic of China Clinical Research center Shanghai Chest HospitalShanghai Jiao Tong UniversityShanghai 200233People’s Republic of China Regenerative Medicine Research center The First People’s Hospital of Yunnan ProvinceThe Affiliated Hospital of Kunming University of Science and TechnologyKunming 650000People’s Republic of China shanghai Institute of Traumatology and Orthopaedics Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint DiseasesRuijin HospitalShanghai Jiao Tong University School of MedicineShanghai 200025People’s Republic of China

Insufficient bionic performance is a structural obstacle and makes urethral repair *** overcome this challenge,we mimicked the urethral matrix and applied two electrospinning techniques to build a double-layer sponge tube of nanofib-ers and ***,silk fibroin(SF)and vitamin B5(VitB5)could be introduced to increase the elasticity of the outer layer and reduce the hydrophobicity to further improve mesenchymal cell *** experiments validated the bionic structure,biocompatibility,and exosome delivery capacity in this *** achieved scarless urethral repair by delivering the bioactive growth factors from adipose-derived stem cell exosomes by physical *** regeneration of the urethra can be accomplished with continuous epithelium in ***,bioinformatics studies revealed that the expression of cell proliferation and fibrotic genes(e.g.,Wnt7a,cfa-miR-574)was responsible for the bio-logical regeneration of the adipose-derived stem cells exosomes(ADSC-exos)by delivering poly l-lactide-co-caprolactone/SF/VitB5 bilayer sponge(PSVBS)via reduced fibrosis gene expression,as well as improved epithelial formation and blood vessel ***,the PSVBS design appeared to be an instructive approach for urethral and other tubular organ regeneration.

关键词： Electrospinning Nanofiber Nanoyarn Urethral stricture Urethral reconstruction

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

HPC-Atlas:Computationally Constructing A Comprehensive Atlas of Human Protein Complexes

引用

Genomics, Proteomics & bioinformatics 2023年第5期21卷 976-990页

作者： Yuliang Pan Ruiyi Li Wengen Li Liuzhenghao Lv Jihong Guan Shuigeng Zhou Department of Computer Science and Technology College of Electronic and Information EngineeringTongji UniversityShanghai 201804China Translational Medical center for Stem Cell Therapy Shanghai East HospitalSchool of MedicineTongji UniversityShanghai 200120China shanghai Key Laboratory of Intelligent Information Processing School of Computer ScienceFudan UniversityShanghai 200433China

A fundamental principle of biology is that proteins tend to form complexes to play important roles in the core functions of *** a complete understanding of human cellular functions,it is crucial to have a comprehensive atlas of human protein ***,we still lack such a comprehensive atlas of experimentally validated protein complexes,which prevents us from gaining a complete understanding of the compositions and functions of human protein complexes,as well as the underlying biological *** fill this gap,we built Human Protein Complexes Atlas(HPC-Atlas),as far as we know,the most accurate and comprehensive atlas of human protein complexes available to *** integrated two latest protein interaction networks,and developed a novel computational method to identify nearly 9000 protein complexes,including many previously uncharacterized *** with the existing methods,our method achieved outstanding performance on both testing and independent ***,with HPC-Atlas we identified 751 severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)-affected human protein complexes,and 456 multifunctional proteins that contain many potential moonlighting *** results suggest that HPC-Atlas can serve as not only a computing framework to effectively identify biologically meaningful protein complexes by integrating multiple protein data sources,but also a valuable resource for exploring new biological *** HPCAtlas webserver is freely available at http://***/HPC-Atlas.

关键词： Human protein complex Protein interaction network SARS-CoV-2-affected complex Multifunctional protein Complex identification method

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Draft Genome of White-blotched River Stingray Provides Novel Clues for Niche Adaptation and Skeleton Formation

引用

Genomics, Proteomics & bioinformatics 2023年第3期21卷 501-514页

作者： Jingqi Zhou Ake Liu Funan He Yunbin Zhang Libing Shen Jun Yu Xiang Zhang School of Public Health Shanghai Jiao Tong University School of MedicineShanghai 200025China Department of Life Sciences Changzhi UniversityChangzhi 046011China Greehey Children’s Cancer Research Institute UT Health San AntonioSan AntonioTX 78229USA State Key Laboratory of Cell Biology Shanghai Key Laboratory of Molecular AndrologyShanghai Institute of Biochemistry and Cell BiologyCenter for Excellence in Molecular Cell ScienceChinese Academy of SciencesShanghai 200031China International Human Phenome Institutes(shanghai) Shanghai 200433China Institute of Neuroscience Center for Excellence in Brain Science and Intelligence TechnologyChinese Academy of SciencesShanghai 200031China Beijing Institute of Genomics Chinese Academy of Sciences and China National Center for BioinformationBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China shanghai Nanmulin Biotechnology Company Limited Shanghai 200031China

The white-blotched river stingray(Potamotrygon leopoldi)is a cartilaginous fish native to the Xingu River,a tributary of the Amazon River *** a rare freshwater-dwelling cartilaginous fish in the Potamotrygonidae family in which no member has the genome sequencing information available,*** provides the evolutionary details in fish phylogeny,niche adaptation,and skeleton *** this study,we present its draft genome of 4.11 Gb comprising 16,227 contigs and 13,238 scaffolds,with contig N50 of 3937 kb and scaffold N50 of 5675 kb in *** analysis shows that *** is a slow-evolving fish that diverged from elephant sharks about 96 million years ***,two gene families related to the immune system(immunoglobulin heavy constant delta genes and T-cell receptor alpha/delta variable genes)exhibit expansion in *** *** also identified the Hox gene clusters in *** and discovered that seven Hox genes shared by five representative fish species are missing in *** RNA sequencing data from *** and other three fish species demonstrate that fishes have a more diversified tissue expression spectrum when compared to *** functional studies suggest that lack of the gc gene encoding vitamin D-binding protein in cartilaginous fishes(both *** and Callorhinchus milii)could partly explain the absence of hard bone in their ***,this genome resource provides new insights into the niche adaptation,body plan,and skeleton formation of ***,as well as the genome evolution in cartilaginous fishes.

关键词： White-blotched river stingray Potamotrygon leopoldi De novo genome assembly Vitamin D-binding protein Niche adaptation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Development of genome-wide insertion/deletion markers in rice based on graphic pipeline platform

引用

Journal of Integrative Plant Biology 2015年第11期57卷 980-991页

作者： Yang L Xiao Cui Rui Li Piaopiao Huang Jie Zong Danqing Yao Gang Li Dabing Zhang Zheng Yuan State Key Laboratory of Hybrid Rice School of Life Sciences and BiotechnologyShanghai Jiao Tong University Novel bioinformatics Company shanghai Agricultural Technology Extension and Service center

DNA markers play important roles in plant breed- ing and genetics. The Insertion/Deletion （InDel） marker is one kind of co-dominant DNA markers widely used due to its low cost and high precision. However, the canonical way of searching for InDel markers is time-consuming and labor- intensive. We developed an end-to-end computational solution （InDel Markers Development Platform, IMDP） to identify genome-wide InDel markers under a graphic pipeline environment. IMDP constitutes assembled genome sequen- ces alignment pipeline （AGA-pipe） and next-generation re- sequencing data mapping pipeline （NGS-pipe）. With AGA-pipe we are able to identify 12,944 markers between the genome of rice cultivars Nipponbare and 93-11. Using NGS-pipe, we reported 34,794 InDels from re-sequencing data of rice cultivars Wu-Yun-Geng7 and Guang-Lu-Ai4. Combining AGA- pipe and NGS-pipe, we developed 2o5,659 InDels in eight japonica and nine indica cultivars and 2,681 InDels showed a subgroup-specific pattern. Polymerase chain reaction （PCR） analysis of subgroup-specific markers indicated that the precision reached 90% （86 of 95）. Finally, to make them available to the public, we have integrated the InDels/markers information into a website （Rice InDel Marker Database, RIMD, http：I/2o2.12o.45.71/）. The application of IMDP in rice will facilitate efficiency for development of genome-wide InDel markers, in addition it can be used in other species with reference genome sequences and NGS data.

关键词： Genetic polymorphism genome alignment InDel marker molecular breeding next-generation sequencing

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Complex networks theory for analyzing metabolic networks

引用

Chinese Science Bulletin 2006年第13期51卷 1529-1537页

作者： ZHAO Jing YU Hong LUO Jianhua CAO Z. W. LI Yixue Department of Biomedical Engineering Shanghai Jiao Tong University Shanghai 200240 China shanghai center for Bioinformation and Technology Shanghai 200235 China shanghai Institutes for Biological Sciences Chinese Academy ofSciences Shanghai 200031 China Department of mathematics Logistical Engineering UniversityChongqing 400016 China

One of the main tasks of post-genomic informatics is to systematically investigate all mole- cules and their interactions within a living cell so as to understand how these molecules and the interactions between them relate to the function of the organism, while networks are appropriate abstract description of all kinds of interactions. In the past few years, great achievement has been made in developing theory of complex networks for revealing the organizing prin- ciples that govern the formation and evolution of various complex biological, technological and social networks. This paper reviews the accomplishments in constructing genome-based metabolic networks and describes how the theory of complex networks is applied to analyze metabolic networks.

关键词：生物信息学系统生物学代谢网络网络拓扑网络负载

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The contribution of cis-regulatory elements to head-to-head gene pairs' co-expression pattern

引用

Science China(Life Sciences) 2009年第1期52卷 74-79页

作者： HAO Pei1,2,YU Yao2,3,ZHANG XiaoYan4,TU Kang2,FAN HaiWei2 & ZHONG Yang1 1 School of Life Sciences,Fudan University,shanghai 200433,China 2 bioinformatics center,Key Lab of Systems Biology,shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,shanghai 200031,China 3 shanghai center for Bioinformation Technology,shanghai 200235,China 4 School of Life Sciences,Tongji University,shanghai 200092,China School of Life Sciences Fudan University Shanghai China bioinformatics center Key Lab of Systems Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China shanghai center for Bioinformation Technology Shanghai China School of Life Sciences Tongji University Shanghai China

Transcription regulation is one of the most critical pipelines in biological process,in which cis-elements play the role as gene expression *** attempt to deduce the principles underlying the co-expression of "head-to-head" gene pairs by analyzing activities or behaviors of the shared cis-elements.A network component analysis was performed to estimate the impact of cis-elements on gene promoters and their activities under different *** discoveries reveal how biological system uses those regulatory elements to control the expression pattern of "head-to-head" gene pairs and the whole transcription regulation system.

关键词： transcription regulation cis-elements "head-to-head" gene pairs

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Bow-tie topological features of metabolic networks and the functional significance

引用

Chinese Science Bulletin 2007年第8期52卷 1036-1045页

作者： ZHAO Jing TAO Lin YU Hong LUO JianHua CAO ZhiWei LI YiXue School of Life Sciences & Technology Shanghai Jiao Tong University Shanghai 200240 China shanghai center for Bioinformation and Technology Shanghai 200235 China shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China Department of Mathematics Logistical Engineering University Chongqing 400016 China

Exploring the structural topology of genome-based large-scale metabolic network is essential for in- vestigating possible relations between structure and *** would be helpful for obtaining immediate information about structural *** this work,metabolic networks of 75 organisms were investigated from a topological point of view.A spread bow-tie model was proposed to give a clear visualization of the bow-tie structure for metabolic *** revealed topological pattern helps to design more efficient algorithm specifically for metabolic *** coarse- grained graph also visualizes the vulnerable connections in the network,and thus could have important implication for disease studies and drug target *** addition,analysis on the reciprocal links and main cores in the GSC part of bow-tie also reveals that the bow-tie structure of metabolic networks has its own intrinsic and significant features which are significantly different from those of random networks.

关键词：代谢网络领结结构拓扑特征生物信息学功能

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Expression of mapk1 and egr1 genes in Onchidium reevesii under tidal stimulation

引用

Aquaculture and Fisheries 2023年第1期8卷 42-49页

作者： Rongyu Wu Rongcheng Rao Xiaoming Zhang Heding Shen Key Laboratory of Aquatic Germplasm Resources Exploration and Utilization of Ministry of Education Shanghai Ocean UniversityShanghai201306China National Experimental Teaching Demonstration center of Aquaculture Science Shanghai Ocean UniversityShanghai201306China Classification and Evolution of Marine Animal System Shanghai Ocean University Key Laboratory of Shanghai UniversitiesShanghai201306China

Previous studies have speculated that tidal rhythm of Onchidium reevesii was linked to its memory ability, which has not been well explained. Mapk1 and egr1 genes are closely related to memory formation in the MAPK signaling pathway that have been extensively studied in vertebrates. These two genes are involved in Long-term potentiation (LTP), which is generally regarded as one of the main molecular mechanisms underlying learning and memory. To investigate the relationship between tidal rhythm of O. reevesii and its memory ability, we studied the molecular mechanism of mapk1 and egr1 genes cooperating with tidal rhythm. The full-length cDNA sequence of mapk1 gene was cloned by RACE method and analyzed by bioinformatics, then qRT-PCR was used to analyze its expression levels in each tissue. A homology analysis and phylogenetic tree reconstruction revealed that O. reevesii is most closely related to the Biomphalaria glabrata. The qRT-PCR showed that mapk1 was expressed in all the tissues, but the highest expression was in the ganglion. We speculated that mapk1 is related to memory formation of O. reevesii. Then we used qRT-PCR to detect the expression of mapk1 and egr1 in ganglion of O. reevesi under tidal stimulation. The expression of mapk1 and egr1 at the rising tide points were significantly higher (P < 0.05) than that at the previous one lowest tide points on May 15-21, except the rising tide point on May 20 and May 21 of mapk1. The expression of mapk1 and egr1 genes in the ganglion sampled for 7-day were basically the same as that of the tide change of the same day. The expression of the two genes were upregulated at raising tide and downregulated at the lowest tide, it is presumed that the O. reevesii was affected by the tide for a long time and formed the tide memory.

关键词： Onchidium reevesii Tide rhythm mapk1 egr1

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Functional analysis of schistosomes EF-hand domain-containing tegument proteins

引用

Chinese Science Bulletin 2007年第15期52卷 2100-2107页

作者： YU FuDong1,2,3,KANG Bin1,3,LI YuanYuan3,4 & LI YiXue1,3 1 bioinformatics center,Key Laboratory of Systems Biology,shanghai Institutes for Biological Sciences,Chinese Academy of Sci-ences,shanghai 200031,China 2 Graduate University of Chinese Academy of Sciences,shanghai 200031,China 3 shanghai center for Bioinformation Technology,shanghai 200235,China 4 Key Laboratory of Systems Biology,shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,shanghai 200031,China bioinformatics center Key Laboratory of Systems Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China Graduate University of Chinese Academy of Sciences Shanghai China shanghai center for Bioinformation Technology Shanghai China Key Laboratory of Systems Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China

Schistosomes cause schistosomiasis disease which severely threatens human health. Little is known about the functions of EF-hand domain containing schistosomes tegument proteins other than as an-tigens. More possible functions of these tegument proteins were investigated with in silico analyses including protein-protein functional interaction,site-specific variation and glycosylation modification. The analysis results suggested that schistosomes could actively modulate host immune responses for its own favor through functional interactions with host proteins with immunomodulatory function,and passively regulate host immune responses through sequence variation under positive selection and glycosylating the recognition sites of host immune attack. In addition,the analysis of the C-terminal domain of these tegument proteins indicated that they could assist schistosomes in escaping host immune attacks through inhibiting chemotaxis and non-complement fixing antibody (IgG4) responses. In summary,our results suggested that these tegument antigen proteins could assist schistosomes in escaping and modulating host immune responses for self-protection during the process of host-para-site interaction.

关键词：寄生虫血吸虫皮蛋白质健康

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Exploring and exploiting genetics and genomicsfor sweetpotato improvement: Status andperspectives

引用

Plant Communications 2022年第5期3卷 38-62页

作者： Mengxiao Yan Haozhen Nie Yunze Wang Xinyi Wang Robert Jarret Jiamin Zhao Hongxia Wang Jun Yang shanghai Key Laboratory of Plant Functional Genomics and Resources Shanghai Chenshan Botanical GardenShanghai 201602China College of Life Sciences Shanghai Normal UniversityShanghai 200234China USDA-ARS/PGRU GriffinGA 30223USA National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 200032China

Sweetpotato(Ipomoea batatas(L.)Lam.)is one of the most important root crops cultivated *** of its adaptability,high yield potential,and nutritional value,sweetpotato has become an important food crop,particularly in developing *** ensure adequate crop yields to meet increasing demand,it is essential to enhance the tolerance of sweetpotato to environmental stresses and other yield-limiting *** highly heterozygous hexaploid genome of *** complicates genetic studies and limits improvement of sweetpotato through traditional ***,application of next-generation sequencing and high-throughput genotyping and phenotyping technologies to sweetpotato genetics and genomics research has provided new tools and resources for crop *** this review,we discuss the genomics resources that are available for sweetpotato,including the current reference genome,databases,and available bioinformatics *** systematically review the current state of knowledge on the polyploid genetics of sweetpotato,including studies of its origin and germplasm diversity and the associated mapping of important agricultural *** then outline the conventional and molecular breeding approaches that have been applied to ***,we discuss future goals for genetic studies of sweetpotato and crop improvement via breeding in combination with state-of-the-art multi-omics approaches such as genomic selection and gene *** approaches will advance and accelerate genetic improvement of this important root crop and facilitate its sustainable global production.

关键词： sweetpotato polyploid genetics genomics breeding

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：