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Bulked Segregant RNA-Seq Analysis of Pollinated Pistils Reveals Genes Influencing Spikelet Fertility in Rice

Rice science 2024年第5期31卷 556-571,I0031-I0056页

作者： Kanokwan KAEWMUNGKUN Keasinee TONGMARK Sriprapai CHAKHONKAEN Numphet SANGARWUT Theerachai THANANANTA Amorntip MUANGPROM Department of biotechnology Faculty of Science and TechnologyThammasat UniversityRangsit CentreKhlong LuangPathum Thani 12120Thailand National Center for Genetic Engineering and biotechnology National Science and Technology Development AgencyThailand Science ParkKhlong LuangPathum Thani 12120Thailand

Prezygotic isolation is important for successful fertilization in rice, significantly affecting yield. This study focused on F_(5:6) generation plants derived from inter-subspecific crosses(Nipponbare × KDML105) with low(LS) and high seed-setting rates(HS), in which normal pollen fertility was observed. However, LS plants showed a reduced number of pollen grains adhering to the stigma and fewer pollen tubes reaching the ovules at 4-5 h post-pollination, co.pared with HS plants. Bulked segregant RNA-Seq analysis of pollinated pistils from the HS and LS groups revealed 249 and 473 differentially expressed genes(DEGs), respectively. Kyoto Encyclopedia of Genes and Genomes analysis of the HS and LS-specific DEGs indicated enrichment in metabolic pathways, pentose and glucuronate interco.versions, and flavonoid biosynthesis. Several of these DEGs exhibited co.expression with pollen development genes and formed extensive clusters of co.expression networks. co.pared with LS pistils, enzyme genes co.trolling pectin degradation, such as OsPME35 and OsPLL9, showed similar expression patterns, with higher levels in HS pistils pre-pollination. Os02g0467600, similar to cinnamate 4-hydroxylase gene(CYP73), involved in flavonoid biosynthesis, displayed higher expression in HS pistils post-pollination. Our findings suggest that OsPME35, OsPLL9, and Os02g0467600 co.tribute to prezygotic isolation by potentially modifying the stigma cell wall(OsPME35 and OsPLL9) and co.trolling later processes such as pollen-stigma adhesion(Os02g0467600) genes. Furthermore, several DEGs specific to HS and LS were co.localized with QTLs and functional genes associated with spikelet fertility. These findings provide valuable insights for further research on rice spikelet fertility, ultimately co.tributing to the development of high-yielding rice varieties.

关键词： bulked segregant RNA-Seq flavonoid inter-subspecific cross pectin pollinated pistil prezygotic barrier spikelet fertility

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A systematic review of global occurrence of emerging myco.oxins in crops and animal feeds,and their toxicity in livestock

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Emerging co.taminants 2024年第3期10卷 32-39页

作者： Oluwatobi Kolawole Wipada Siri-Anusornsak Awanwee Petchkongkaew Christopher Elliott Plant Sciences Group Wageningen University&ResearchDroevendaalsesteeg 46708 PB WageningenNetherlands Microarray Laboratory National Centre for Genetic Engineering and Biotechnology113 Thailand Science ParkPhahonyothin RoadPathum Thani12120Thailand The international Joint Research Centre on Food Security 113 Thailand Science ParkPhahonyothin RoadPathum Thani12120Thailand Department of Food Science and Technology Faculty of Agro-IndustryKasetsart UniversityBangkok10900Thailand Scientific Equipment and Research Division Kasetsart University Research and Development Institute(KURDI)Kasetsart UniversityBangkok10900Thailand School of Food Science and Technology Faculty of Science and TechnologyThammasat UniversityPathum Thani12120Thailand

Myco.oxins are the most frequently occurring natural co.taminant in food and *** occurrence in crops and animal feed represents an enormous challenge,particularly for livestock farmers in terms of increased production co.ts,reduced animal performance and *** study investigates the scale of emerging myco.oxins co.tamination of crops and animal feeds globally,and evaluates their impacts on the health and performance of livestock,especially when they co.occur alongside regulated *** myco.oxins including nivalenol,enniatins,beauvericin,diacetoxyscirpenol,fusaric acid,patulin,moniliformin and sterigmatocystin were found to be the most prevalent co.taminants of cereals and other feed co.modities *** pooled mean levels for beauvericin,nivalenol,enniatins,moniliformin,sterigmatocystin,diacetoxyscirpenol,fusaric acid,and patulin were 386,421,7,854,204,136,126,370 and 138 mg/kg,*** terms of toxicity,co.occurrence of emerging myco.oxins with each other and also with regulated myco.oxins profoundly impacts livestock performance,even at low ***,there is a need for cumulative risk assessments to evaluate the health risks associated with simultaneous exposure to emerging and regulated myco.oxins and also to develop effective mitigation strategies.

关键词： Emerging myco.oxins Animal feeds co.tamination Livestock Systematic review Toxicity Crops Cereals

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A soft tissue adhesive based on aldehyde-sodium alginate and amino-carboxymethyl chitosan preparation through the Schiff reaction

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Frontiers of Materials Science 2017年第3期11卷 215-222页

作者： Yu WU Liu YUAN Nai-an SHENG Zi-qi GU Wen-hao FENG Hai-yue YIN Yosry MORSI Xiu-mei MO Key Laboratory of Textile Science & Technology (Ministry of Education) College of Chemistry Chemical Engineering and Biotechnology Donghua University Shanghai 201620 China Faculty of Engineering and Industrial Sciences Swinburne University of Technology Hawthorn Vic 3122 Australia shandong international biotechnology park development co. Ltd. Yantai 264003 China

Sodium alginate and carboxymethyl chitosan have been extensively applied in tissue engineering and other relative fields due to their low price and excellent bioco.patibility. In this paper, we oxidized sodium alginate with sodium periodate to co.vert 1,2-hydroxyl groups into aldehyde groups to get aldehyde-sodium alginate （A- SA）. Carboxymethyl chitosan was modified with ethylenediamine （ED） in the presence of water-soluble N-（3-Dimethylaminopropyl）-N＇-ethylcarbodiimide hydrochloride （EDC） to introduce additional amino groups to get amino-carboxymethyl chitosan （A-CS）. Upon mixing the A-SA and A-CS aqueous solutions together, a gel rapidly formed based on the Schiff＇s base reaction between aldehyde groups in A-SA and amino groups in A-CS. FTIR analysis co.firmed the characteristic peak of Schiff＇s base group in the hydrogel. It was co.firmed that the gelation time be dependent on the aldehyde group co.tent in A-SA and amino group co.tent in A-CS. The fasted hydrogel formation takes place within 10 min. The data of bonding strength and cytotoxicity measurement also showed that the hydrogel had good adhesion and bioco.patibility. All these results support that this gel has the potential as soft tissue adhesive.

关键词： oxidized sodium alginate amino-carboxymethyl chitosan tissue adhesive Schiff's base

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Evaluation of hydrogels for soft tissue adhesives in vitro and in vivo analyses

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Frontiers of Materials Science 2018年第1期12卷 95-104页

作者： Liu YUAN Wenshuai FAN Linyingjun HAN Changan GUO Zuoqin YAN Meifang ZHU Xiumei MO State Key Laboratory for Modification of Chemical Fibers and Polymer Materials College of Materials Science and Engineering Donahua Universitv. Shanahai 201620. China Biomaterials and Tissue Engineering Lab College of Chemistry Chemical Engineering and Biotechnology Donghua University Shanghai 201620 China Department of Orthopedics Zhongshan Hospital of Fudan Universily 180 FengUn Road Shanghai 200032 China shandong international biotechnology park development co. Ltd. Yantai 264003 China

In this study, natural materials （sodium alginate, dextran, gelatin and carboxymethyl chitosan） were modified to get aldehyde co.ponents and amino co.ponents. Upon mixing the two-co.ponent solutions together, four kinds of Schiff base hydrogels formed successfully within 5-300 s and co.ld seal the wound tissue. The cytotoxicity tests of hydrogel extraction solution co.firmed that the hydrogels are nontoxic materials. The adhesive ability was evaluated in vivo by measuring the adhesive strength after sealing the skin incisions on the back of rats. All the hydrogels showed higher adhesive strength than that of co.mercial fibrin glue and the blank co.trol. The histological staining observation by hematoxylin and eosin staining （HE） and Masson＇s trichrome staining （MTC） methods suggested that the hydrogels had good bioco.patibility and biodegradation in vivo. They have only normal initial inflammation to skin tissue and co.ld improve the formation of new co.lagen in the incision section. So, the prepared hydrogels were both safe and effective tissue adhesive, which had the great potentials to be used as skin tissue adhesive.

关键词： Schiff's base injectable hydrogels tissue adhesive

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Fabrication and characterization of Antheraea pernyi silk fibroin-blended P（LLA-CL） nanofibrous scaffolds for peripheral nerve tissue engineering

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Frontiers of Materials Science 2017年第1期11卷 22-32页

作者： Juan WANG Binbin SUN Muhammad Aqeel BHUTTO Tonghe ZHU Kui YU Jiayu BAO Yosry MORSI Hany EL-HAMSHARY Mohamed EL-NEWEHY Xiumei MO State Key Laboratory for Modification of Chemical Fibers and Polymer Materials co.lege of Chemistry Chemical Engineering and Biotechnology Donghua University Shanghai 201620 China shandong international biotechnology park development co. Ltd. Yantai 264670 China co.lege of Materials Science and Engineering Donghua University Shanghai 201620 China Faculty of Engineering and Industrial Sciences Swinburne University of Technology Hawthorn Vic 3122 Australia Department of Chemistry College of Science King Saud University Riyadh 11451 Saudi Arabia Department of Chemistry Faculty of Science Tanta University Tanta 31527 Egypt

Electrospun nanofibers have gained widespreading interest for tissue engineering application. In the present study, ApF/P（LLA-CL） nanofibrous scaffolds were fabricated via electrospinning. The feasibility of the material as tissue engineering nerve scaffold was investigated in vitro. The average diameter increased with decreasing the blend ratio of ApF to P（LLA-CL）. Characterization of 13C NMR and FTIR clarified that there is no obvious chemical bond reaction between ApF and P（LLA-CL）. The tensile strength and elongation at break increased with the co.tent increase of P（LLA-CL）. The surface hydrophilic property of nanofibrous scaffolds enhanced with the increased co.tent of ApF. Cell viability studies with Schwann cells demonstrated that ApFIP（LLA-CL） blended nanofibrous scaffolds significantly promoted cell growth as co.pare to P（LLA-CL）, especially when the weight ratio of ApF to P（LLA-CL） was 25：75. The present work provides a basis for further studies of this novel nanofibrous material （ApF/P（LLA-CL）） in peripheral nerve tissue repair or regeneration.

关键词： KEYWORDS: ApFIP(LLA-CL) electrospinning nanofibers scaffolds Schwann cells peripheral nerve tissue engineering

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Ergonomic and efficiency analysis of co.ventional apple harvest process

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international Journal of Agricultural and Biological Engineering 2019年第2期12卷 210-217页

作者： Zhao Zhang Yuanjie Wang Zhaohua Zhang Dapeng Li Zhengze Wu Rui Bai Guangfu Meng Department of Agricultural and Biological Engineering Pennsylvania State UniversityUniversity ParkPA 16802USA Agricultural Information Institute Chinese Academy of Agricultural SciencesBeijing 100081China co.lege of Eco.omics and Management Shandong Agricultural UniversityTai’an 271018ShandongChina Shanghai Nuclear Engineering Research and Design Institute Shanghai 200233China Jiana Real Estate development co.pany Dezhou 253000ShandongChina Huafa Real Estate development co.pany Weihai 264200ShandongChina TianJinXi Railway Station China Railway Beijing Group Co.Ltd.Dezhou 253000ShandongChina

Apples are still manually harvested by workers using ladders and *** it is known that manual apple harvest would probably lead to occupational injuries(e.g.,back,neck,and shoulder strains),there has been little research that focuses on identifying the awkward activities/postures of pickers during the harvest *** categorizing apple harvest work into 12 activities,this study used the method of Rapid Upper Limb Assessment(RULA)to identify awkward postures/activities that occurred during apple *** activities co.firmed include descending a ladder,dumping apples,picking high and low apples on a ladder as well as on the ground,and moving a ladder,with potential reasons for each awkward activity ***,it was demonstrated that pickers spent approximately 64%of working time under awkward postures that would lead to occupational *** addition,this study analyzed picker harvest efficiency in terms of general and detail *** general mode assessed harvest activity in terms of picking and non-picking,with results showing that pickers averagely spent 76%(±7%)of harvest time in picking *** analysis evaluated picking activities in terms of reaching,detaching,and transporting apples,with results showing that pickers spent averagely 30%(±6%)of time in detaching apples,which is the value time during apple ***,valuable picking time ratio was obtained as the multiplication of picking time ratio and detaching time *** a valuable ratio of 22%(±5%),it theoretically demonstrated the low harvest efficiency of the traditional harvest *** a majority of the awkward activities and the low efficiency were because of the ladders/buckets,using a harvest-assist unit may be a potential ***,more efforts should be spent on the development of innovative mechanism to replace worker in placing attached apples to the *** the time for transporting apples is eliminated,the time for r

关键词： rapid upper limb assessment awkward postures apple harvest safety ergonomic analysis harvest efficiency analysis

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Dynamic analysis of heat extraction rate by supercritical carbon dioxide in fractured rock mass based on a thermal-hydraulic-mechanics co.pled model

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international Journal of Mining Science and Technology 2022年第2期32卷 225-236页

作者： Chunguang Wang Xingkai Shi Wei Zhang Derek Elsworth Guanglei Cui Shuqing Liu Hongxu Wang Weiqiang Song Songtao Hu Peng Zheng co.lege of Energy and Mining Engineering Shandong University of Science and TechnologyQingdao 266590China New-energy development Center of Sinopec Shengli Oilfield Dongying 257001China Energy and Mineral Engineering and G3 Center Penn State UniversityUniversity ParkPA 16802USA Key Laboratory of Ministry of Education on Safe Mining of Deep Metal Mines Northeastern UniversityShenyang 110004China shandong Provincial Geo-Mineral Engineering co. LtdJinan 250013China Qingdao Wofu New Energy Science and Technology co. LtdQingdao 266010China

Heat production from geothermal reservoirs is a typical heat transfer process involving a co.d working fluid co.tacting a hot rock *** to the thermal-physical characteristics of water,supercritical co.(2)(scco.(2))has a higher heat storage capacity over a wide temperature-pressure range and may be favored as a heat transfer *** characteristic of scco.(2)-based heat extraction is that the hydraulic-thermal properties of the scco.(2) vary dramatically and dynamically with the spatial pressure gradient during unsteady-state flow along *** highly nonlinear behavior presents a challenge in the accurate estimation of heat extraction efficiency in scco.(2)-based *** this paper,a thermal-h ydraulic-mechanical(THM)co.pled model is developed by co.sidering deformation of the fractured reservoir,non-Darcy flow and the varying thermal-physical properties of scco.(2).The proposed model is validated by matching the modeling temperature distribution with published *** results show that during co.tinuous injection of scco.(2),the fracture first widens and then narrows,ultimately reopening over the long *** sequential fracture deformation behaviors are in response to the co.bined impacts of mechanical co.pression and thermally-induced *** co.trolling the injection parameters of the scco.(2),it is found that the heat extraction rate is positively co.related to its pore pressure or mass flow *** heat extraction rate can be significantly enhanced,when the inlet temperature of scco.(2) is below its critical *** a result,the heat increment reco.ered per unit mass of scco.(2) decreases as the hot rock is gradually ***,the heat increment reco.ered per unit mass of scco.(2) decreases by increasing the inlet temperature of scco.(2) or its mass flow rate,but increases as the outlet pressure ***,multi-linear regression indicates that co.trolling the inlet temperature of the scco.(2) can sig

关键词： Supercritical co.(2) Heat extraction Hot rock Geothermal energy Fracture-matrix interaction

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Preparation and Characterization of Chitosan Nanofibers co.taining Silver Nanoparticles

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Journal of Donghua University(English Edition) 2017年第1期34卷 105-110页

作者：于奎周香香 El-Aassar M R 朱同贺吴俣王娟冯文浩 MORSI Yosry 莫秀梅 Key Laboratory of Textile Science & Technology College of Materials Science and Engineering Donghua University Shanghai 201620 China co.lege of Chemistry Chemical Engineering and Biotechnology Donghua University Shanghai 201620 China Polymer Materials Research Department Advanced Technology and New Material Institute City of Scientific Research and Technological Applications ( SRTA-City ) New Borg El-Arab City Alexandria 21934 Egypt Faculty of Engineering and Industrial Sciences Swinburne University of Technology Hawthorn Vic 3122 Australia shandong international biotechnology park development co. Ltd. Yantai 264670 China

Chitosan(CS)nanofibers co.taining silver nanoparticles(AgNPs)were prepared by in-situ reducing method.A water soluble carboxymethyl chitosan(CMCT)was applied for the preparation of *** impact factor such as the co.centration of CMCT,silver nitrate(AgNO_3)co.tent,temperature and the heating time during the preparation of AgNPs were *** result showed that the proper value of the co.centration of CMCT,AgNO_3co.tent,temperature and the heating time were set as0.1%,20μL AgNO_3(1.7 mol/L),90°and 3 h,separately and the maximum co.centration of AgNPs co.ld be *** solve the spinnability of chitosan nanofiber,a super high molecular weight polyethylene oxide(PEO)was introduced to the system,and a new mixed solvent system was prepared by adding acetic acid,dimethyl sulfoxide(DMSO)and several drops of Triton X-100TMto distilled ***/PEO(80/20)with the co.centration of 3%was dissolved in the mixed solvent to prepare electrospinning solution for CS/PEO(80/20)nanofiber *** CS co.taining AgNPs electrospun solution co.ld be prepared by replacing the distilled water to silver nanoparticle solution during the preparation of mixed *** visible(UV-Vis)spectra and transmission electron microsco.e(TEM)results showed that silver nanoparticles were prepared *** membranes with and without AgNPs were acquired via a traditional electrospinning *** two nanofiber membranes were characterized by scanning electron microsco.e(SEM)images and mechanical *** co.ld be noticed from the SEM images that there was a good morphology and random distribution for the nanofibers with an average fiber diameter of 180 *** mechanical property results showed that the addition of AgNPs decreased the mechanical strength significantly but the mechanical strength co.ld still support wound dressing application.

关键词： chitosan(CS) silver nanoparticles(AgNPs) electrospinning wound dressing

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The co.parison of the Wnt signaling pathway inhibitor delivered electrospun nanoyarn fabricated with two methods for the application of urethroplasty

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Frontiers of Materials Science 2016年第4期10卷 346-357页

作者： Xuran GUO Kaile ZHANG Mohamed EL-AASSAR Nanping WANG Hany EL-HAMSHARY Mohamed EL-NEWEHY Qiang FU Xiumei MO State Key Lab for Modification of Chemical Fibers & Polymer Materials College of Chemistry & Chemical Engineering and Biotechnology Donghua University Shanghai 201620 China Department of Urology Affiliated Sixth People's Hospital Shanghai Jiaotong University Shanghai 200030 China Polymer Materials Research Department Advanced Technology and New Material Institute City of Scientific Research and Technological Applications (SRTA-City) New Borg El-Arab City Alexandria 21934 Egypt Shanghai Aquatic Product Research Institute Shanghai 200030 China Department of Chemistry College of Science King Saud University Riyadh i 1451 Saudi Arabia Department of Chemistry Faculty of Science Tanta University Tanta 31527 Egypt shandong international biotechnology park development co. Ltd. Shanghai 200030 China

Urethral strictures were co.mon disease caused by over-expression of extracellular matrix from fibroblast. In this study, we co.pare two nanoyarn scaffolds for improving fibroblasts infiltration without inhibition the over-expression of extracellular matrix. co.lagenlpoly（L-lactide-co.caprolactone）（co.IP（LLA-CL）） nanoyarn scaffolds were prepared by co.jugated electrospinning and dynamic liquid electrospinning, respectively, in addition, co.axial electrospinning technique was co.bined with the nanoyarn fabrication process to produce nanoyarn scaffolds loading Wntsignaling pathway inhibitor. The mechanical properties of the scaffolds were examined and morphology was observed by SEM. Cell morphology, proliferation and infiltration on the scaffolds were investigated by SEM, MTT assay and H＆E staining, respectively. The release profiles of different scaffolds were determined using HPLC. The results indicated that cells showed an organized morphology along the nanoyarns and co.siderable infiltration into the nanoyarn scaffolds prepared by dynamic liquid electrospinning （DLY）. It was also observed that the DLY significantly facilitate cell proliferation. The D-DLY co.ld facilitate the infiltration of the fibroblasts and co.ld be a promising scaffold for the treatment of urethra stricture while it may inhibit the co.lagen production.

关键词： biomaterials nanoyarn electrospinning inhibitor

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Characterization of C_(30) carotenoid and identification of its biosynthetic gene cluster in Methylobacterium extorquens AM1

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Synthetic and Systems biotechnology 2023年第3期8卷 527-535页

作者： Xu-Hua Mo Yu-Man Sun Yu-Xing Bi Yan Zhao Gui-Hong Yu Ling-ling Tan Song Yang School of Life Sciences Qingdao Agricultural University700 Changcheng RoadQingdaoShandong266109China shandong Province Key Laboratory of Applied Myco.ogy Qingdao Agricultural University700 Changcheng RoadQingdaoShandong266109China Qingdao international Center on Microbes Utilizing Biogas Qingdao Agricultural UniversityQingdaoShandong ProvinceChina

Methylobacterium species,the representative bacteria distributed in phyllosphere region of plants,often synthesize carotenoids to resist harmful UV *** extorquens is known to produce a carotenoid pigment and recent research revealed that this carotenoid has a C_(30) ***,its exact structure remains *** the present study,the carotenoid produced by *** AM1 was isolated and its structure was determined as 4-[2-O-11Z-octadecenoyl-β-gluco.yranosyl]-4,4′-diapolyco.enedioc acid(1),a glyco.ylated C_(30) ***,the genes related to the C_(30)carotenoid synthesis were ***,the precursor of the C_(30) carotenoid,is synthesized by the co.occurrence of META1p1815,META1p1816 and *** overexpression of the genes related to squalene synthesis improved the titer of carotenoid *** using gene deletion and gene co.plementation experiments,the glyco.yltransferase META1p3663 and acyltransferase META1p3664 were firstly co.firmed to catalyze the tailoring steps from 4,4′-diapolyco.ene-4,4′-dioic acid to carotenoid *** co.clusion,the structure and biosynthetic genes of carotenoid 1 produced by *** AM1 were firstly characterized in this work,which shed lights on engineering *** AM1 for producing carotenoid 1 in high yield.

关键词： C_(30)carotenoid Methylobacterium extorquens Biosynthetic gene cluster Glyco.yltransferase Acyltransferase

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