检索结果-南通市图书馆

Integrated multi-omics reveals contrasting epigenetic patterns in leaf and root morphogenesis in Aegilops speltoides

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The Crop Journal 2025年第1期13卷 292-298页

作者： Lingfeng Miao Zhe Chen Lifeng Gao Jizeng Jia Weilong Guo Guangyao Zhao Key Laboratory of Crop gene Resources and Breeding the National Key Facility for Crop Gene Resources and Genetic ImprovementInstitute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China Frontiers Science Center for Molecular Design Breeding Key Laboratory of Crop Heterosis and UtilizationBeijing Key Laboratory of Crop Genetic ImprovementChina Agricultural UniversityBeijing 100193China

Aegilops speltoides,the closest ancestor of the wheat B subgenome,has been well studied ***,the epigenetic landscape of *** and the effects of epigenetics on its growth and development remain poorly ***,we present a comprehensive multi-omics atlas of leaves and roots in ***,encompassing transcriptome,DNA methylation,histone modifications,and small RNA *** DNA methylation levels were detected between leaves and roots,and were associated with differences in accumulated 24-nt *** methylation changes in promoters and gene bodies showed strong connections with altered expression between leaves and *** regulatory networks(TRN)reconstructed between leaves and roots were driven by tissue-specific TF *** methylation and histone modification act together as switches that shape root and leaf morphogenesis by modulating the binding of tissue-specific TFs to their target *** TRNs in leaves and roots reshaped during wheat polyploidization were associated with alterations in epigenetic ***,these results not only shed light on the critical contribution of epigenetic regulation in the morphogenesis of leaves and roots in *** but also provide new insights for future investigations into the complex interplay of genetic and epigenetic factors in the developmental biology of common wheat.

关键词： Aegilops speltoides DNA methylation Histone modification Transcriptional regulatory network

Identification of a NF-кB site in the negative regulatory element (ε-NRAII) of human ε-globin gene and its binding protein NF-кB p50 in the nuclei of K562 cells

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cell Research 2002年第1期12卷 79-82页

作者： CHUN Hui HOU, JIAN HUANG, Ruo LAN QIAN Group of Globin gene expression and regulation, State Key Labortory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 1Group of Globin gene expression and regulation State Key Labortory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China 1Group of Globin gene expression and regulation State Key Labortory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China 1Group of Globin gene expression and regulation State Key Labortory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China

The developmental control of the human e-globin gene expression is mediated by transcription regulatory elements in the 5’ flanking DNA of this gene. Sequence analysis has revealed a DNA motif (GGGGAATTTGCT) similar to NF-кB consensus sequence resides in the negative regulatory element (-3028bp～ -2902bp, termed ε-NRAII) 5’ to the cap site of this gene. NRF DNA fragment (-3010bp～ -2986bp) containing the NF-кB motif similar sequence was synthesized and used in electrophoresis mobility shift assay (EMSA) and competitive analysis. Data showed that a protein factor from nuclear extracts of K562 cells specifically interacted with NRF DNA fragment. The synthetic NF DNA fragment (containing NF-кB consensus sequence) could competed for the protein binding, but MNF DNA fragment (mutated NF-кB motif) could not, suggesting that the binding protein is a member of NF-кB/Rel family. Western blot assay demonstrated that the molecular weight of NF-кB protein in the nuclei of K562 cells is 50ku. We suggested that NF-кB p50 may play an important role in the regulation of human c-globin gene expression.

关键词： Human ε-globin gene negative regulatory element NE-кB p50.

The in vitro reconstitution of nucleosome and its binding patterns with HMG1/2 and HMG14/17 proteins

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Cell Research 2003年第5期13卷 351-359页

作者： SHU BING ZHANG, JIAN HUANG, HUI ZHAO, YI ZHANG, CHUN HuI HOU, XIAO DONG CHENG, CHU JIANG, MIN QIAN LI, JUN HU, RUO LAN QIAN 1 Group of Globin gene expression and regulation, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China. 2 Shanghai Institute of Nuclear Research, Chinese Academy of Sciences, Shanghai 201800, China Group of Globin gene expression and regulation State Key Laboratory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China

Using atomic force microscopy (AFM), the dynamic process of the in vitro nucleosome reconstitution followed by slow dilution from high salt to low salt was visualized. Data showed that the histone octamers were dissociated from DNA at 1M NaCl. When the salt concentration was slowly reduced to 650 mM and 300 mM, the core histones bound to the naked DNA gradually. Once the salt concentration was reduced to 50 mM the classic 'beads-on-astring' structure was clearly visualized. Furthermore, using the technique of the in vitro reconstitution of nucleosome, the mono- and di- nucleosomes were assembled in vitro with both HS2core (-10681 to -10970 bp) and NCR2 (-372 to -194 bp) DNA sequences in the 5’flanking sequence of human b-globin gene. Data revealed that HMG 1/2 and HMG 14/17 proteins binding to both DNA sequences are changeable following the assembly and disassembly of nucleosomes. We suggest that the changeable binding patterns of HMG 14/17 and HMG1/2 proteins with these regulatory elements may be criti

关键词： atomic force microscopy HMG proteins (HMG1/2 and HMG14/17) human β-globin gene in vitro reconstitution nucleosome.

Hot spots of DNA double-strand breaks and genomic contacts of human rDNA units are involved in epigenetic regulation

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Journal of Molecular Cell Biology 2015年第4期7卷 366-382页

作者： Nickolai A.Tchurikov Daria M.Fedoseeva Dmitri V.Sosin Anastasia V.Snezhkina Nataliya V.Melnikova Anna V.Kudryavtseva Yuri V.Kravatsky Olga V.Kretova Department of Epigenetic Mechanisms of gene expression regulation Engelhardt Institute of Molecular BiologyMoscow 119334Russia Group of Postgenomic Studies Engelhardt Institute of Molecular BiologyMoscow 119334Russia Laboratory of DNA-Protein Interactions Engelhardt Institute of Molecular BiologyMoscow 119334Russia

DNA double-strand breaks(DSBs)are involved in many cellular mechanisms,including replication,transcription,and genome *** recent observation that hot spots of DSBs in human chromosomes delimit DNA domains that possess coordinately expressed genes suggests a strong relationship between the organization of transcription patterns and hot spots of *** this study,we performed mapping of hot spots of DSBs in a human 43-kb ribosomal DNA(rDNA)repeated *** observed that rDNA units corresponded to the most fragile sites in human chromosomes and that these units possessed at least nine specific regions containing clusters of extremely frequently occurring DSBs,which were located exclusively in non-coding intergenic spacer(IGS)*** hot spots of DSBs corresponded to only a specific subset of DNase-hypersensitive sites,and coincided with CTCF,PARP1,and HNRNPA2B1 binding sites,and H3K4me3 *** rDNA-4C data indicate that the regions of IGS containing the hot spots of DSBs often form contacts with specific regions in different chromosomes,including the pericentromeric regions,as well as regions that are characterized by H3K27ac and H3K4me3 marks,CTCF binding sites,ChIA-PET and RIP signals,and high levels of *** data suggest a strong link between chromosome breakage and several different mechanisms of epigenetic regulation of gene expression.

关键词： double-strand breaks fragile sites rDNA IGS PARP1 HNRNPA2B1 4C

Thyroid hormone regulation of apoptotic tissue remodeling during anuran metamorphosis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cell Research 2001年第4期11卷 245-252页

作者： SHI YUN-Bo, LIEZHEN FU, SHAO CHUNG VICTOR HSIA, AKIHIRO TOMITA, DANIEL BUCHHOLZ Laboratory of gene regulation and Development, National Institute of Child Health and Human Develop- ment, National Institutes of Health, Bethesda, Maryland 20892-5431, USA 1Laboratory of gene regulation and Development National Institute of Child Health and Human Development National Institutes of Health Bethesda Maryland 20892-5431 USA 1Laboratory of gene regulation and Development National Institute of Child Health and Human Development National Institutes of Health Bethesda Maryland 20892-5431 USA 1Laboratory of gene regulation and Development National Institute of Child Health and Human Development National Institutes of Health Bethesda Maryland 20892-5431 USA 1Laboratory of gene regulation and Development National Institute of Child Health and Human Development National Institutes of Health Bethesda Maryland 20892-5431 USA 1Laboratory of gene regulation and Development National Institute of Child Health and Human Development National Institutes of Health Bethesda Maryland 20892-5431 USA

Anuran metamorphosis involves systematic transformations of individual organs in a thyroid hormone (TH)-dependent manner. Morphological and cellular studies have shown that the removal of larval or- gans/tissues such the tail and the tadpole intestinal epithelium is through programmed cell death or apop- tosis. Recent molecular investigations suggest that TH regulates metamorphosis by regulating target gene expression through thyroid hormone receptors (TRs), which are DNA-binding transcription factors. Cloning and characterization of TH response genes show that diverse groups of early response genes are induced by TH. The products of these TH response genes are believed to directly or indirectly affect the expression and/or functions of cell death genes, which are conserved at both sequence and function levels in different animal species. A major challenge for future research lies at determining the signaling pathways leading to the activation of apoptotic processes and whether different death genes are involved in the regulation of apoptosis in different tissues/organs to effect tissue-specific transformations.

关键词： Xenopus laevis thyroid hormone receptor extracellular matrix apoptosis.

TERC suppresses PD-L1 expression by downregulating RNA binding protein HuR

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2022年第12期65卷 2505-2516页

作者： Heping Jin Yanlian Chen Jian Ren Junjiu Huang Yong Zhao Haiying Liu MOE Key Laboratory of gene Function and regulation School of Life SciencesSun Yat-sen UniversityGuangzhou 510006China

TERC is the RNA component of telomerase,and provides a template for TERT to synthesize telomere repeats at chromosome *** evidence has revealed that TERC is involved in other biological processes beyond ***,we found that the expression level of TERC is negatively correlated with PD-L1 and that ectopic expression of TERC but not TERT in ALT cells significantly inhibits PD-L1,suggesting that TERC suppresses PD-L1 expression in a telomerase-independent ***,instead of regulating PD-L1 m RNA directly,TERC accelerates PD-L1 m RNA degradation by inhibiting the expression of Hu R,which binds to the 3′UTR of PD-L1 m RNA and maintains its *** also found that the small molecule AS1842856,a Fox O1 inhibitor,promotes TERC expression and reverses the PD-L1 upregulation caused by chemotherapy,providing a potential combination cancer therapy that avoids cancer immune escape during chemotherapy.

关键词： TERC PD-L1 HuR FoxO1 inhibitor

维普期刊数据库

Distinct expression profiles of transcriptional coactivators for thyroid hormone receptors during Xenopus laevis metamorphosis

在线全文

学校读者我要写书评

暂无评论

Cell Research 2003年第6期13卷 459-464页

作者： BINDU D PAUL National Institutes of Health Laboratory of gene regulation and Development National Institute of Child Health and Human Development Bldg. USA.

The biological effects of thyroid hormone (T3) are mediated by the thyroid hormone receptor (TR). Amphibian metamorphosis is one of the most dramatic processes that are dependent on T3. T3 regulates a series of orchestrated developmental changes, which ultimately result in the conversion of an aquatic herbivorous tadpole to a terrestrial carnivorous frog. T3 is presumed to bind to TRs, which in turn recruit coactivators, leading to gene activation. The best-studied coactivators belong to the p160 or SRC family. Members of this family include SRC1/ NCoA-1, SRC2/TIF2/GRIP1, and SRC3/pCIP/ACTR/AIB-l/RAC-3/TRAM-1. These SRCs interact directly with liganded TR and function as adapter molecules to recruit other coactivators such as p300/CBP. Here, we studied the expression patterns of these coactivators during various stages of development. Amongst the coactivators cloned in Xenopus laevis, SRC3 was found to be dramatically upregulated during natural and T3-induced metamorphosis, and SRC2 and p300 are express

关键词： transcription coactivators thyroid hormone receptor Xenopus laevis metamorphosis histone acetylation.

Non-coding RNAs in cancers with chromosomal rearrangements:the signatures,causes,functions and implications

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Journal of Molecular Cell Biology 2019年第10期11卷 886-898页

作者： Cai Han Lin-Yu Sunf Wen-Tao Wang Yu-Meng Sun Yue-Qin Chen MOE Key Laboratory of gene Function and regulation State Key Laboratory for BiocontrolSun Yat-sen UniversityGuangzhou 510275China

Chromosomal translocation leads to the juxtaposition of two otherwise separate DNA loci,which could result in gene *** rearrangements at the DNA level are catastrophic events and often have causal roles in *** oncogenic DNA messages are transferred to RNA molecules,which are in most cases translated into cancerous fusion *** expression programs and signaling pathways are altered in these cytogenetically abnormal ***,non-toding RNAs have attracted increasing attention and are believed to be tightly associated with chromosome-rearranged *** RNAs not only function as modulators in downstream pathways but also directly affect chromosomal translocation or the associated *** review summarizes recent research advances on the relationship between non-coding RNAs and chromosomal translocations and on diverse functions of non-coding RNAs in cancers with chromosomal rearrangements.

关键词： chromosomal translocation non-coding RNA fusion protein gene regulation non-coding fusion transcript

Beneficial metabolic activities of inflammatory cytokine interleukin 15 in obesity and type 2 diabetes

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Frontiers of Medicine 2015年第2期9卷 139-145页

作者： Jianping Ye Antioxidant and gene regulation Laboratory Pennington Biomedical Research Centel Louisiana State University System Baton Rouge LA70808 USA

In obesity, chronic inflammation is believed to induce insulin resistance and impairs adipose tissue function. Although this view is supported by a large body of literature, it has been challenged by growing evidence that pro-inflammatory cytokines may favor insulin sensitivity through induction of energy expenditure. In this review article, interleukin 15 （IL-15） is used as a new example to explain the beneficial effects of the pro- inflammatory cytokines. IL-15 is secreted by multiple types of cells including macrophages, neutrophils and skeletal muscle cells. IL-15 expression is induced in immune cells by endotoxin and in muscle cells by physical exercise. Its transcription is induced by transcription factor NF-KB. IL-15 binds to its receptor that contains three different subunits （~, [5 and 1,） to activate JAK/STAT, PI3K/Akt, IKK/NF-KB and JNK/API pathways in cells. In the regulation of metabolism, IL-15 reduces weight gain without inhibiting food intake in rodents. IL-15 suppresses lipogenesis, stimulates brown fat function, improves insulin sensitivity through weight loss and energy expenditure. In human, circulating IL-15 is negatively associated with body weight. In the immune system, IL-15 stimulates proliferation and differentiation of T cells, NK cells, monocytes and neutrophils. In the anti-obesity effects of IL-15, T cells and NK cells are not required, but leptin receptor is required. In summary, evidence from human and rodents supports that the pro-inflammatory cytokine IL-15 may enhance energy expenditure to protect the body from obesity and type 2 diabetes. The mechanism of IL-15 action remains to be fully uncovered in the regulation of energy expenditure.

关键词： inflammation obesity cytokine energy expenditure insulin resistance