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Journal of Biomedical Research 2024年第3期38卷 278-286页

作者： Vishal P.Dubey Varun P.Sureja Dharmeshkumar B.Kheni Department of scientific and Medical affairs Sundyota Numandis Probioceuticals Pvt.Ltd.AhmedabadGujarat 380015India

Menopause is characterized by various physical,mental and emotional *** 731®is a standardized extract from Rheum rhaponticum root and has been clinically studied for its role in reducing menopausal *** current systematic review and meta-analysis aimed to evaluate the efficacy of ERr 731®supplementation in alleviating the severity of menopausal *** this review,we searched across three online databases up to March 2023,evaluated the quality of the included studies by the Physiotherapy Evidence Database scale,and assessed the risk of bias by the Cochrane Risk of Bias *** then performed a metaanalysis using RevMan software to estimate the pooled mean difference(MD).The study protocol was registered in the Prospective Register of Systematic Reviews(CRD42023416808).After screening and evaluation,we included four high-quality studies(a total of 390 participants;the ERr 731®group:193 participants;the control group:197 participants)in the *** results showed that ERr 731®supplementation significantly reduced the Menopause Rating Scale score(MD:–15.12;P<0.001),compared with control *** analysis revealed no effect of individual studies on the overall pooled estimate or overall observed *** current review provides evidence that ERr 731®supplementation is effective in reducing menopause *** bias and high heterogeneity in the results warrant further clinical studies.

关键词： menopause Rheum rhaponticum hot flashes systematic review

A nanobody-based blocking enzyme-linked immunosorbent assay for detecting antibodies against pseudorabies virus glycoprotein E

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Journal of Integrative Agriculture 2024年第4期23卷 1354-1368页

作者： Huanhuan Lü Pinpin Ji Siyu Liu Ziwei Zhang Lei Wang Yani Sun Baoyuan Liu Lizhen Wang Qin Zhao Department of Preventive Veterinary Medicine College of Veterinary MedicineNorthwest A&F University/Shaanxi Scientific Observing and Experimental Station of Veterinary Pharmacology and Diagnostic TechnologyMinistry of Agriculture and Rural AffairsYangling 712100China

Pseudorabies(PR)is an acute infectious disease of pigs caused by the PR virus(PRV)and results in great economic losses to the pig industry *** glycoprotein E(gE)-based enzyme-linked immunosorbent assay(ELISA)has been used to distinguish gE-deleted vaccine-immunized pigs from wild-type virus-infected pigs to eradicate PR in some *** has the advantages of small size and easy genetic engineering and has been a promising diagnostic ***,there were few reports about developing nanobody-based ELISA for detecting anti-PRV-gE *** the present study,the recombinant PRV-gE was expressed with a bacterial system and used to immunize the Bactrian ***,two nanobodies against PRV-gE were screened from the immunized camel by phage display ***,two nanobody-HRP fusion proteins were expressed with HEK293T *** PRV-gE-Nb36-HRP fusion protein was selected as the probe for developing the blocking ELISA(bELISA)to detect anti-PRV-gE *** optimizing the conditions of bELISA,the amount of coated antigen was 200 ng per well,and dilutions of the fusion protein and tested pig sera were separately 1:320 and 1:*** cut-off value of bELISA was 24.20%,and the sensitivity and specificity were 96.43 and 92.63%,*** detecting 233 clinical pig sera with the developed bELISA and a commercial kit,the results showed that the coincidence rate of two assays was 93.99%.Additionallly,epitope mapping showed that PRV-gE-Nb36 recognized a conserved conformational epitope in different reference PRV ***,great stability and low-cost nanobody-based bELISA for detecting anti-PRV-gE antibodies were *** bELISA could be used for monitoring and eradicating PR.

关键词： nanobody nanobody-HRP blocking ELISA PRV-gE antibody

Genetic analysis and candidate gene identification of salt tolerancerelated traits in maize

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Journal of Integrative Agriculture 2024年第7期23卷 2196-2210页

作者： Hui Fang Xiuyi Fu Hanqiu Ge Mengxue Jia Jie Ji Yizhou Zhao Zijian Qu Ziqian Cui Aixia Zhang Yuandong Wang Ping Li Baohua Wang scientific Observing and Experimental Station of Maize in Plain Area of Southern Region Ministry of Agriculture and Rural Affairs/School of Life SciencesNantong UniversityNantong 226019China Maize Research Institute Beijing Academy of Agriculture and Forestry Sciences(BAAFS)Beijing 100097China

Soil salinization poses a threat to maize production worldwide,but the genetic mechanism of salt tolerance in maize is not well ***,identifying the genetic components underlying salt tolerance in maize is of great *** the current study,a teosinte-maize BC2F7 population was used to investigate the genetic basis of 21 salt tolerance-related *** total,125 QTLs were detected using a high-density genetic bin map,with one to five QTLs explaining 6.05–32.02%of the phenotypic variation for each *** total phenotypic variation explained(PVE)by all detected QTLs ranged from 6.84 to 63.88%for each *** all 125 QTLs,only three were major QTLs distributed in two genomic regions on chromosome 6,which were involved in three salt tolerance-related *** addition,10 pairs of epistatic QTLs with additive effects were detected for eight traits,explaining 0.9 to 4.44%of the phenotypic ***,18 QTL hotspots affecting 3–7 traits were *** one hotspot(L5),a gene cluster consisting of four genes(ZmNSA1,SAG6,ZmCLCg,and ZmHKT1;2)was found,suggesting the involvement of multiple pleiotropic ***,two important candidate genes,Zm00001d002090 and Zm00001d002391,were found to be associated with salt tolerance-related traits by a combination of linkage and marker-trait association ***00001d002090 encodes a calcium-dependent lipid-binding(CaLB domain)family protein,which may function as a Ca^(2+)sensor for transmitting the salt stress signal downstream,while Zm00001d002391 encodes a ubiquitin-specific protease belonging to the C19-related *** findings provide valuable insights into the genetic basis of salt tolerance-related traits in maize and a theoretical foundation for breeders to develop enhanced salt-tolerant maize varieties.

关键词： maize salt tolerance-related traits QTL mapping region-based association analysis

Assessing the yield difference of double-cropping rice in South China driven by radiation use efficiency

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Journal of Integrative Agriculture 2024年第11期23卷 3692-3705页

作者： Jian Lu Sicheng Deng Muhammad Imran Jingyin Xie Yuanyuan Li Jianying Qi Shenggang Pan Xiangru Tang Meiyang Duan State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/College of Agriculture South China Agricultural University scientific Observing and Experimental Station of Crop Cultivation in South China Ministry of Agriculture and Rural Affairs Guangzhou Key Laboratory for Science and Technology of Fragrant Rice

Double-cropping rice in South China continues to break the total yield record,but the yield potential of singlecropping rice is not being *** use efficiency （RUE） has been singled out as an important determinant of grain yield in many cereal ***,there is no information on whether the yield gaps in doublecropping rice involve differences in *** experiments were performed over two years to evaluate the effects of intercepted radiation （IP） and RUE on the above-ground biomass production,crop growth rate （CGR）,and harvest index （HI）,in four representative rice varieties,i.e.,Xiangyaxiangzhan （XYXZ）,Meixiangzhan 2 （MXZ2）,Nanjingxiangzhan （NJXZ）,and Ruanhuayoujinsi （RHYJS）,during the early and late seasons of rice cultivation in South *** results revealed that grain yield in the early season was 8.2%higher than in the late *** yield advantage in the early season was primarily due to higher spikelets per panicle and above-ground biomass resulting from a higher *** spikelets per panicle in the early season were 6.5,8.3,6.9,and 8.5%higher in XYXZ,MXZ2,NJXZ,and RHYJS,respectively,than in the late *** higher early season grain yield was more closely related to RUE in the middle tillering stage （R2=0.34）,panicle initiation （R2=0.16）,and maturation stage（R2=0.28）,and the intercepted photosynthetically active radiation （IPAR） in the maturation stage （R2=0.28）,while the late season grain yield was more dependent on IPAR in the middle tillering stage （R2=0.31） and IPAR at panicle initiation （R2=0.23）.The results of this study conclusively show that higher RUE contributes to the yield progress of early season rice,while the yield improvement of late season rice is attributed to higher radiation during the early reproductive *** allocating the RUE of double-cropping rice with high RUE varieties or adjustments of the sowing period merits further study.

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Membrane vesicles derived from Streptococcus suis serotype 2 induce cell pyroptosis in endothelial cells via the NLRP3/Caspase-1/GSDMD pathway

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Journal of Integrative Agriculture 2024年第4期23卷 1338-1353页

作者： Keda Shi Yan Li Minsheng Xu Kunli Zhang Hongchao Gou Chunling Li Shaolun Zhai Institute of Animal Health Guangdong Academy of Agricultural Sciences/Key Laboratory of Livestock Disease Prevention ofGuangdong Province/Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of GuangdongProvinceMinistry of Agriculture and Rural AffairsGuangzhou 510640China Maoming Branch Guangdong Laboratory for Lingnan Modern Agricultural Science and TechnologyMaoming 525000China College of Animal Science&Technology Zhongkai University of Agriculture and EngineeringGuangzhou 510225China

Streptococcus suis serotype 2(*** 2)is a zoonotic pathogen that clinically causes severe swine and human infections(such as meningitis,endocarditis,and septicemia).In order to cause widespread diseases in different organs,*** 2 must colonize the host,break the blood barrier,and cause exaggerated *** the last few years,most studies have focused on a single virulence factor and its influences on the *** vesicles(MVs)can be actively secreted into the extracellular environment contributing to bacteria-host ***-negative bacteria-derived outer membrane vesicles(OMVs)were recently shown to activate host Caspase-11-mediated non-canonical inflammasome pathway via deliverance of OMV-bound lipopolysaccharide(LPS),causing host cell ***,little is known about the effect of the MVs from *** 2(Gram-positive bacteria without LPS)on cell ***,we investigated the molecular mechanism by which *** 2 MVs participate in endothelial cell *** this study,we used proteomics,electron scanning microscopy,fluorescence microscope,Western blotting,and bioassays,to investigate the MVs secreted by *** ***,we demonstrated that *** 2 secreted MVs with an average diameter of 72.04 nm,and 200 proteins in MVs were ***,we showed that MVs were transported to cells via mainly dynamin-dependent *** *** 2 MVs activated NLRP3/Caspase-1/GSDMD canonical inflammasome signaling pathway,resulting in cell pyroptosis,but it did not activate the Caspase-4/-5 *** importantly,endothelial cells produce large amounts of reactive oxygen species(ROS)and lost their mitochondrial membrane potential under induction by *** 2 *** results in this study suggest for the first time that MVs from *** 2 were internalized by endothelial cells via mainly dynamin-dependent endocytosis and might promote NLRP3/Caspase-1/GSDMD pathway by mitochondrial damage,which produced mtDNA and ROS under induction,

关键词： Streptococcus suis serotype 2 membrane vesicles endocytosis pyroptosis NLRP3 inflammasomes mitochondrial damage endothelial cell

Artificial enhancement of sturgeon stock in freshwater reservoirs:A case study on sterlet Acipenser ruthenus of the Kama reservoir

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Aquaculture and Fisheries 2024年第2期9卷 287-294页

作者： Pavel B.Mikheev Semyon N.Kazarinov Alla G.Melnikova Stanislav V.Ponosov Nikolai G.Petrenko Andrei I.Nikiforov Alexey Yu Puzik Olga N.Elchenkova Perm State National Research University Perm614990Russia Khabarovsk Branch of the Federal State Budget scientific Institution“Russian Federal Research Institute of Fisheries and Oceanography”“KhabarovskNIRO” Khabarovsk680038Russia Perm Branch of the Federal State Budget scientific Institution“Russian Federal Research Institute of Fisheries and Oceanography”“PermNIRO” Perm614991Russia Federal State Budget scientific Institution“Russian Federal Research Institute of Fisheries and Oceanography”“VNIRO” Moscow117454Russia Moscow State Institute of International Relations(University)of the Ministry for Foreign affairs of Russia“MGIMO” Moscow117454Russia Department of State Control Supervision and Protection of Aquatic Biological Resources for Perm KraiMiddle-Volga Regional Office of the Federal Fishery AgencyPerm614990Russia

The paper presents the results of the sterlet sturgeon Acipenser ruthenus reintroduction into the Kama Reservoir(Perm Krai,Russia)by sterlet fry from hatcheries into the reservoir since *** effectiveness of hatchery releases was evaluated by monitoring gillnet sampling *** results showed that the species was reported throughout entire Kama Reservoir,which contrasts with 1988-1994 data,when the species was registered only at the upper part of the reservoir near the Kama River *** highest number and biomass values of catch per unit effort(CPUE)of the sterlet were found in submerged river channels-the deepest habitats with flow conditions which are close to the ecological requirements of the *** distribution of the sterlet within the channel biotopes of the reservoir is defined by reduced *** biggest individuals and the widest size range of the sterlet were typical for the upper area of the reservoir and Chusovskoy Bay,which have the greatest *** smallest sizes were typical for fish from catches near the dam of the reservoir,which is consistent with the published data on the distribution of sterlet in reservoir *** presented results may indicate the artificial formation of the sterlet stock in the Kama Reservoir maintained by hatchery *** revealed distribution of the sterlet in the reservoir determines the direction of further research on natural reproduction and thus formation of an artificially formed ***,future studies are required to assess the possibility of hybridization of the sterlet of artificial origin with natural populations of the species,since the low genetic diversity of the sterlet released into the Kama Reservoir which can lead to irreversible loss of genetic individuality of the natural stocks of the species in the catchment of upper course of the Kama River.

关键词： Sterlet Acipenser ruthenus Kama reservoir Hatchery releases CPUE Artificial stock formation

Improving model performance in mapping cropland soil organic matter using time-series remote sensing data

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Journal of Integrative Agriculture 2024年第8期23卷 2820-2841页

作者： Xianglin Zhang Jie Xue Songchao Chen Zhiqing Zhuo Zheng Wang Xueyao Chen Yi Xiao Zhou Shi Institute of Applied Remote Sensing and Information Technology College of Environmental and Resource SciencesZhejiang UniversityHangzhou 310058China Department of Land Management Zhejiang UniversityHangzhou 310058China ZJU-Hangzhou Global scientific and Technological Innovation Center Zhejiang UniversityHangzhou 311215China Institute of Digital Agriculture Zhejiang Academy of Agricultural SciencesHangzhou 310021China Key Laboratory of Spectroscopy Sensing Ministry of Agriculture and Rural AffairsHangzhou 310058China

Faced with increasing global soil degradation,spatially explicit data on cropland soil organic matter(SOM)provides crucial data for soil carbon pool accounting,cropland quality assessment and the formulation of effective management *** a spatial information prediction technique,digital soil mapping(DSM)has been widely used to spatially map soil information at different ***,the accuracy of digital SOM maps for cropland is typically lower than for other land cover types due to the inherent difficulty in precisely quantifying human *** overcome this limitation,this study systematically assessed a framework of“information extractionfeature selection-model averaging”for improving model performance in mapping cropland SOM using 462 cropland soil samples collected in Guangzhou,China in *** results showed that using the framework of dynamic information extraction,feature selection and model averaging could efficiently improve the accuracy of the final predictions(R^(2):0.48 to 0.53)without having obviously negative impacts on *** the dynamic information of the environment was an efficient way to generate covariates that are linearly and nonlinearly related to SOM,which improved the R^(2)of random forest from 0.44 to 0.48 and the R^(2)of extreme gradient boosting from 0.37to *** recursive feature selection(FRFS)is recommended when there are relatively few environmental covariates(500).The Granger-Ramanathan model averaging approach could improve the prediction accuracy and average *** the structures of initial prediction models are similar,increasing in the number of averaging models did not have significantly positive effects on the final *** the advantages of these selected strategies over information extraction,feature selection and model averaging have a great potential for high-accuracy soil mapping

关键词： cropland soil organic matter digital soil mapping machine learning feature selection model averaging

Nucleophiles promotes the decomposition of electrophilic functional groups of tetracycline in ZVI/H2O2 system: Efficiency and mechanism

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Chinese Chemical Letters 2024年第10期35卷 440-446页

作者： Xin Li Wanting Fu Ruiqing Guan Yue Yuan Qinmei Zhong Gang Yao Sheng-Tao Yang Liandong Jing Song Bai Key Laboratory of Pollution Control Chemistry and Environmental Functional Materials for Qinghai-Tibet Plateau of the National Ethnic affairs Commission School of Chemistry and Environment Southwest Minzu University Key Laboratory of General Chemistry of the National Ethnic affairs Commission School of Chemistry and Environment Southwest Minzu University Institute of Environmental Engineering RWTH Aachen University

When zero-valent iron（ZVI） is prepared and applied under neutral conditions, it is easy to form oxides or hydroxides on its surface, which hinders the electron release of ZVI. To this end, a nucleophile was introduced into the ZVI system to inhibit the precipitation of iron ions, improve the conductivity of the solution, and promote the removal efficiency of electrophilic functional groups in organic compounds. In this study, the addition of nucleophiles such as ethylenediamine, methylamine and dimethylamine to the ZVI/H2O2system resulted in an enhanced removal efficiency of tetracycline（TC） under neutral condition,while electrophiles such as EDTA-2Na and oxalic acid dihydrate impeded the removal of TC. Experimental results demonstrated that the presence of nucleophiles could effectively promote the release of iron ions and increase the proportion of ferrous in both aqueous solution and solid surface of ZVI. Experimental and theoretical calculation results revealed that the electrophilic functional group was eliminated in the TC molecule, and the toxicity of the treated solution was reduced significantly. Overall, this work provides a selection of the conditions and pollutants applicable to ZVI under neutral pH conditions.

关键词： Electronic transfer Electrophilic functional group Nucleophiles Tetracycline ZVI

A mite parasitoid,Pyemotes zhonghuajia,negatively impacts the fitness traits and immune response of the fall armyworm,Spodoptera frugiperda

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Journal of Integrative Agriculture 2024年第1期23卷 205-216页

作者： Yanfei Song Tai’an Tian Yichai Chen Keshi Zhang Maofa Yang Jianfeng Liu Institute of Entomology Guizhou Provincial Key Laboratory for Agricultural Pest Management of the Mountainous RegionGuizhou University/Scientific Observing and Experiment Station of Crop Pests in GuiyangMinistry of Agriculture and Rural AffairsGuiyang 550025China Administration of Guizhou Fanjingshan National Nature Reserve Jiangkou 554400China Centre for Biodiversity and Biosecurity School of Biological SciencesThe University of AucklandAuckland 1072New Zealand College of Tobacco Science Guizhou UniversityGuiyang 550025China

Parasitoids are key regulators in ecological communities and widely used as agents in biocontrol *** fall armyworm,Spodoptera frugiperda,recently invaded multiple continents and caused substantial economic losses in *** zhonghuajia,a newly identified mite parasitoid,has shown potential for controlling various agricultural insect ***,this study tested the performance of *** in parasitising *** also investigated the sublethal effects of parasitism by *** on host fitness traits,transgenerational impacts,and cellular and humoral *** result showed that the fifth-instar larvae of *** parasitised by 40 *** were all dead(i.e.,a lethal effect),while parasitism by 5 or 10 *** was considered sublethal since many *** survived to adulthood and produced offspring after *** sublethal influences from parasitism by *** resulted in reduced pupal weight,adult emergence rate and fecundity,but increased developmental time and *** at both lethal(40 mites)and sublethal(10 mites)levels impaired the cellular and humoral immunity of *** study presents the first empirical evidence that mite parasitoids can negatively influence host ***,it provides insights into the biocontrol potential of mite parasitoids and their interactions with hosts.

关键词： Pyemotes zhonghuajia Spodoptera frugiperda immunity parasitically-induced influence biocontrol parasitoid

Interaction Between Ustilaginoidea virens and Rice and Its Sustainable Control

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Rice science 2024年第3期31卷 269-284页

作者： ZHANG Fengmin CAO Zhenzhen ZHENG Xin HE Yuntao CHEN Mingxue LIN Xiaoyan Rice Product Quality Inspection&Supervision Testing Center of Ministry of Agriculture and Rural affairs China National Rice Research InstituteHangzhou 310006China

Ustilaginoidea virens is a common rice pathogen that can easily lead to a decline in rice quality and the production of toxins pose potential risks to human *** this review,we present a comprehensive literature review of research since the discovery of rice false *** provide a comprehensive and,at times,critical overview of the main results and findings from related research,and propose future research ***,we delve into the interaction between *** and rice,including the regulation of transcription factors,the process of *** infecting rice panicles,and the plant immune response caused by rice *** that,we discuss the identification and characterization of mycotoxins produced by the pathogenic fungus,as well as strategies for disease *** emphasize the importance of comprehensive agricultural prevention and control methods for the sustainable management of *** knowledge will update our understanding of the interaction between *** and rice plants,offering a valuable perspective for those interested in ***.

关键词： rice false smut flower-specific infection mycotoxin food safety plant disease