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Food science and Human Wellness 2024年第1期13卷 506-516页

作者： Zhongai Chen Hong Chen Huan Du Cong Chen Kaixiang Lu Qiaoli Xue Yongjin Hu College of Food science and technology Yunnan Agricultural UniversityKunming 650000China Institute of Food Processing Guizhou Academy of Agricultural SciencesGuiyang 550006China Editorial department of Journal of yunnan agricultural university Yunnan Agricultural UniversityKunming 650000China

Sanchuan ham is appreciated in yunnan Province,China,for its characteristic flavor and taste,while the microbial community structure and biogenic amines content remain unclear during fermentation processes.In this study,we explored the physicochemical property,biogenic amines concentration and microbial diversity of external and internal Sanchuan ham by high-throughput sequencing during the processing of Sanchuan ham.Results showed that the nitrite remained at a stable level of 0.15 mg/kg which was significantly lower than the national health standard safety level of 20 mg/kg.In addition,compared with fresh hams,the content of total free amino acids in ripe Sanchuan ham has grown 14 folds;sour and bitter were the main tastes of Sanchuan ham.Notably,the concentration of cadaverine was the highest of all biogenic amines during the entire fermentation period.At the bacterial phyla level,Firmicutes and Actinobacteria were the two main phyla,while at the genus level,Staphylococcus was a significant strain throughout the whole fermentation.Moreover,the dry stage has a great impact on the succession change of microbial community structure.Simultaneously,the change trends and composition of bacteria in the interior have slight discrepancies with those of the exterior of Sanchuan ham.

关键词： Sanchuan ham Nitrite cont ent Free amino acids Cadaverine Staphylococcus

Exploring the material basis and mechanism of Moringa oleifera in alleviating slow transit constipation based on network pharmacology and animal models

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Food science and Human Wellness 2024年

作者： Sijin Li Xiaoyu Gao Kaifeng Guo Shuangfeng Liu Weiqian Yang Yan Zhao Jun Sheng Zhongbin Bai Yang Tian College of Food science and technology yunnan agricultural university yunnan Key Laboratory of Precision Nutrition and Personalized Food Manufacturing yunnan agricultural university department of science and technology yunnan agricultural university College of Veterinary Medicine yunnan agricultural university Engineering Research Center of Development and Utilization of Food and Drug Homologous Resources Ministry of Education yunnan agricultural university

Moringa oleifera(M. oleifera) have laxative effects， but their active compositions and mechanisms are not very clear thus far. To this end， we systematically explored the active components and mechanism of M. oleifera leaves in relieving constipation by using the slow transit constipation (STC) mouse model and network pharmacology. The results of animal experiments showed that M. oleifera aqueous extract (MOA) had good laxative activity， and its 70% alcohol soluble part (ASP) also showed significant laxative activity(P < 0.01). Network pharmacological prediction results suggested that L-phenylalanine (Phe) was the key compound of ASP， and it might relieve constipation through tachykinin receptor 1 (TACR1) and three kinds of adrenergic receptors， including alpha-1a (ADRA1A)， alpha-2a (ADRA2A)， and alpha-2b (ADRA2B). Further animal experiment results showed that Phe significantly promoted gastrointestinal motility. Phe may relieve STC by enhancing the release of substance P (SP) and upregulating the mRNA expression of TACR1 in the ileum. Importantly， Phe may also promote intestinal movement by downregulating ADRA2A and ADRA2B and upregulating calmodulin and the mRNA and protein expression of myosin light chain 9 (MYL9) in the ileum， thereby activating the GPCR-MLC signaling pathway. These results lay a foundation for the application of M. oleifera and Phe in constipation.

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Exploration of Cyclooxygenase-2 (COX-2) Inhibitory Peptides from Walnut Dreg Proteins Based on In Silico and In Vitro Assessments

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Food science and Human Wellness 2023年

作者： Zi-Shan Hong Jing Xie Liang Tao Jing-Jing Dai Ting-Ting Li Li Zhang Yu-Ying Bai Xia Hu Jin-Lian Chen Jun Sheng Yang Tian College of Food science and technology， yunnan agricultural university Puer university Key Laboratory of Puer Tea science， Ministry of Education， yunnan agricultural university Engineering Research Center of Development and Utilization of Food and Drug Homologous Resources， Ministry of Education， yunnan agricultural university yunnan Provincial Engineering Research Center for Edible and Medicinal Homologous Functional Food， yunnan agricultural university National Research and Development Professional Center for Moringa Processing technology， yunnan agricultural university

Walnut dreg protein hydrolysates (WDPHs) exhibit a variety of biological activities， however， the COX-2 inhibitory peptide of WDPHs remain unclear. This aim of this study was to rapidly screen for such peptides in WDPHs through a combination of in silico and in vitro analysis. In total， 1262 peptide sequences were observed by nano LC-MS/MS and four novel COX-2 inhibitory peptides (AGFP， FPGA， LFPD， and VGFP) were identified for the first time. Enzyme kinetic data indicated that AGFP， FPGA， and LFPD displayed mixed-type COX-2 inhibition， whereas VGFP was a non-competitive inhibitor. This is mainly because the peptides form hydrogen bonds and hydrophobic interactions with residues in the COX-2 active site. These results demonstrate that computer analysis combined with in vitro evaluation allows for rapid screening of COX-2 inhibitory peptides in walnut protein dregs.

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Exploration of cyclooxygenase-2 inhibitory peptides from walnut dreg proteins based on in silico and in vitro analysis

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Food science and Human Wellness 2024年第3期13卷 1636-1644页

作者： Zishan Hong Jing Xie Liang Tao Jing-Jing Dai Tingting Li Li Zhang Yuying Bai Xia Hu Jinlian Chen Jun Sheng Yang Tian College of Food science and technology Yunnan Agricultural UniversityKunming 650201China yunnan Provincial Key Laboratory of Precision Nutrition and Personalized Food Manufacturing Yunnan Agricultural UniversityKunming 650201China Engineering Research Center of Development and Utilization of Food and Drug Homologous Resources Ministry of EducationYunnan Agricultural UniversityKunming 650201China School of Tea and Coffee Pu’er UniversityPu’er 665000China yunnan Provincial Engineering Research Center for Edible and Medicinal Homologous Functional Food Yunnan Agricultural UniversityKunming 650201China Key Laboratory of Pu-er Tea science Ministry of EducationYunnan Agricultural UniversityKunming 650201China

Walnut dreg protein hydrolysates(WDPHs)exhibit a variety of biological activities,however,the cyclooxygenase-2(COX-2)inhibitory peptide of WDPHs remain unclear.The aim of this study was to rapidly screen for such peptides in WDPHs through a combination of in silico and in vitro analysis.In total,1262 peptide sequences were observed by nano liquid chromatography/tandem mass spectrometry(nano LC-MS/MS)and 4 novel COX-2 inhibitory peptides(AGFP,FPGA,LFPD,and VGFP)were identified.Enzyme kinetic data indicated that AGFP,FPGA,and LFPD displayed mixed-type COX-2 inhibition,whereas VGFP was a non-competitive inhibitor.This is mainly because the peptides form hydrogen bonds and hydrophobic interactions with residues in the COX-2 active site.These results demonstrate that computer analysis combined with in vitro evaluation allows for rapid screening of COX-2 inhibitory peptides in walnut protein dregs.

关键词： Walnut dreg proteins Cyclooxygenase-2 inhibitory peptide Identification Virtual screening Molecular docking

Phenolic compounds from Moringa oleifera Lam.: Extraction， separation and identification， biological activities， and comprehensive applications

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Food science and Human Wellness 2023年

作者： Liang Tao Zi-Lin Wang Min Yang Xin-Rui Kang Jia-He Dai Cun-Chao Zhao Tian-Yi Dai Jun Sheng Yang Tian Puer university National Research and Development Professional Center for Moringa Processing technology yunnan agricultural university Engineering Research Center of Development and Utilization of Food and Drug Homologous Resources Ministry of Education yunnan agricultural university yunnan Provincial Engineering Research Center for Edible and Medicinal Homologous Functional Food yunnan agricultural university College of Food science and technology yunnan agricultural university

Moringa oleifera Lam. (M. oleifera Lam) is a Moringa genus in the Moringaceae family that is high in nutrients and has a wide range of applications. Phenolic compounds are widely found in plants and have various health benefits for the human body. With its high content and wide variety of phenolic compounds， M. oleifera Lam has been widely studied for its health benefits. The phenolic compounds in M. oleifera Lam (MOPCs) can be a potential source of functional food ingredients in pharmaceutical and industrial applications. Numerous studies have shown that MOPCs have antioxidant， anti-obesity， anti-diabetic， and antibacterial effects. Although the research on MOPCs has been gradually increasing， the extraction， isolation， identification， biological activities， and comprehensive application of MOPCs need a more systematic summary and generalization. Therefore， this paper reviews the isolation and extraction methods， structure identification， biological activities， and comprehensive applications to provide a further reference for the research and application of MOPCs.

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Muscle Biological Characteristics of Differentially Expressed Genes in Wujin and Landrace Pigs

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Journal of Integrative Agriculture 2014年第10期13卷 2236-2242页

作者： XU Hong HUANG Ying LI Wei-zhen YANG Ming-hua GE Chang-rong ZHANG Xi LI Liu-an GAO Shi-zheng ZHAO Su-mei School of Public Finance and Economics Yunnan University of Finance and Economics yunnan Key Laboratory of Animal Nutrition and Feed science Yunnan Agricultural University Faculty of Animal science and technology Yunnan Agricultural University Animal Husbandry Post-Doctoral Station Nanjing Agricultural University department of Animal science Tianjin Agricultural University

The biological chemistry would be responsible for the meat quality. This study tried to investigate the transcript expression profile and explain the characteristics of differentially expressed genes between the Wujin and Landrace pigs. The results showed that 526 differentially expressed genes were found by comparing the transcript expression profile of muscle tissue between Wujin and Landrace pigs. Among them, 335 genes showed up-regulations and 191 genes showed down-regulations in Wujin pigs compared with the Landrace pigs. Gene ontology （GO） analysis indicated that the differentially expressed genes were clustered into three groups involving in protein synthesis, energy metabolism and immune response. Kyoto encyclopedia of genes and genomes （KEGG） pathway analysis found that these differentially expressed genes participated in protein synthesis metabolism, energy metabolism and immune response pathway. The Database for Annotation, Visualization and Integrated Discovery （DAVID） analysis of protein function and protein domains function also confirmed that differentially expressed genes belonged to protein synthesis, energy metabolism and immune response. Genes related protein synthesis metabolism pathway in Landrace was higher than in Wujin pigs. However, differentially expressed genes related energy metabolism and immune response was up-regulated in Wujin pigs compared with Landrace pigs. Quantitative real-time RT-PCR on selected genes was used to confirm the results from the microarray. These suggested that the genes related to protein synthesis, energy metabolism and immune response would contribute to the growth performance, meat quality as well as anti-disease capacity.

关键词： pigs muscle tissue microarray bioinformatics analysis differentially expressed genes

FedCDR:Privacy-preserving federated cross-domain recommendation

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Digital Communications and Networks 2022年第4期8卷 552-560页

作者： Dengcheng Yan Yuchuan Zhao Zhongxiu Yang Ying Jin Yiwen Zhang School of Computer science and technology Anhui UniversityHefeiChina Weifang Key Laboratory of Blockchain on agricultural Vegetables Weifang University of Science and TechnologyWeifangChina department of management Hefei UniversityHefeiChina

Cross-Domain Recommendation(CDR)aims to solve data sparsity and cold-start problems by utilizing a relatively information-rich source domain to improve the recommendation performance of the data-sparse target domain.However,most existing approaches rely on the assumption of centralized storage of user data,which undoubtedly poses a significant risk of user privacy leakage because user data are highly privacy-sensitive.To this end,we propose a privacy-preserving Federated framework for Cross-Domain Recommendation,called FedCDR.In our method,to avoid leakage of user privacy,a general recommendation model is trained on each user's personal device to obtain embeddings of users and items,and each client uploads weights to the central server.The central server then aggregates the weights and distributes them to each client for updating.Furthermore,because the weights implicitly contain private information about the user,local differential privacy is adopted for the gradients before uploading them to the server for better protection of user privacy.To distill the relationship of user embedding between two domains,an embedding transformation mechanism is used on the server side to learn the cross-domain embedding transformation model.Extensive experiments on real-world datasets demonstrate that ourmethod achieves performance comparable with that of existing data-centralized methods and effectively protects user privacy.

关键词： Cross-domain recommendation Federated learning Privacy preserving

Deciphering the chromatin spatial organization landscapes during BMMSC differentiation

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Journal of Genetics and Genomics 2023年第4期50卷 264-275页

作者： Zhaowei Teng Yun Zhu Da Lin Qinggang Hao Qiaoning Yue Xiaochao Yu Shuo Sun Lihong Jiang Sheng Lu department of Orthopedics The First People's Hospital of Yunnan ProvinceAffiliated Hospital of Kunming University of Science and TechnologyKunmingYunnan 650032China Key Laboratory of yunnan Provincial Innovative Application of Traditional Chinese Medicine The First People's Hospital of Yunnan ProvinceKunmingYunnan 650032China Clinical Medical Research Center The First People's Hospital of Yunnan ProvinceKunmingYunnan 650032China The Sixth Affiliated Hospital of Kunming Medical university YuxiYunnan 653100China State Key Laboratory of agricultural Microbiology Huazhong Agricultural UniversityWuhanHubei 430070China Center for Life sciences School of Life SciencesYunnan UniversityKunmingYunnan 650504China

The differentiation imbalance in bone marrow mesenchymal stem cells(BMMSCs)is critical for the development of bone density diseases as the population ages.BMMSCs are precursor cells for osteoblasts and adipocytes;however,the chromatin organization landscapes during BMMSC differentiation remain elusive.In this study,we systematically delineate the four-dimensional genome and dynamic epigenetic atlas of BMMSCs by RNA sequencing,assay for transposase-accessible chromatin sequencing,and highthroughput chromosome conformation capture.The structure analyses reveal 17.5%common and28.5%-30%specific loops among BMMSCs,osteoblasts,and adipocytes.The subsequent correlation of genome-wide association studies and expression quantitative trait locus(e QTL)data with multi-omics analysis reveal 274 genes and 3634 single nucleotide polymorphisms(SNPs)associated with bone degeneration and osteoporosis(OP).We hypothesize that SNP mutations affect transcription factor(TF)binding sites,thereby affecting changes in gene expression.Furthermore,26 motifs,260 TFs,and 291SNPs are identified to affect the e QTL.Among these genes,DAAM2,TIMP2,and TMEM241 are found to be essential for diseases such as bone degeneration and OP and may serve as potential drug targets.

关键词： Bone marrow mesenchymal stem cells Differentiation Hi-C ATAC-Seq Single nucleotide polymorphisms Genome-wide association studies

2,3-Butanediol from the leachates of pine needles induces the resistance of Panax notoginseng to the leaf pathogen Alternaria panax

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Plant Diversity 2023年第1期45卷 104-116页

作者： Tian-Yao Li Chen Ye Yi-Jie Zhang Jun-Xing Zhang Min Yang Xia-Hong He Xin-Yue Mei Yi-Xiang Liu You-Yong Zhu Hui-Chuan Huang Shu-Sheng Zhu School of Agriculture Yunnan UniversityKunming650500China State Key Laboratory for Conservation and Utilization of Bio-Resources in yunnan Yunnan Agricultural UniversityKunming650201China Key Laboratory of Agro-Biodiversity and Pest management of Education Ministry of China Yunnan Agricultural UniversityKunming650201China Southwest Forestry university Kunming650224China

Compared with the use of monocultures in the field,cultivation of medicinal herbs in forests is an effective strategy to alleviate disease.Chemical interactions between herbs and trees play an important role in disease suppression in forests.We evaluated the ability of leachates from needles of Pinus armandii to induce resistance in Panax notoginseng leaves,identified the components via gas chromatography-mass spectrometry(GC-MS),and then deciphered the mechanism of 2,3-Butanediol as the main component in the leachates responsible for resistance induction via RNA sequencing(RNA-seq).Prespraying leachates and 2,3-Butanediol onto leaves could induce the resistance of P.notoginseng to Alternaria panax.The RNA-seq results showed that prespraying 2,3-Butanediol onto leaves with or without A.panax infection upregulated the expression of large number of genes,many of which are involved in transcription factor activity and the mitogen-activated protein kinase(MAPK) signaling pathway.Specifically,2,3-Butanediol spraying resulted in jasmonic acid(JA)-mediated induced systemic resistance(ISR) by activating MYC2 and ERF1.Moreover,2,3-Butanediol induced systemic acquired resistance(SAR) by upregulating pattern-triggered immunity(PTI)-and effector-triggered immunity(ETI)-related genes and activated camalexin biosynthesis through activation of WRKY33.Overall,2,3-Butanediol from the leachates of pine needles could activate the resistance of P.notoginseng to leaf disease infection through ISR,SAR and camalexin biosynthesis.Thus,2,3-Butanediol is worth developing as a chemical inducer for agricultural production.

关键词： Pinus armandii Allelopathy Herbs Induce resistance Diversity Leaf disease

Distribution of heavy metals in water and sediment of an urban river in a developing country:A probabilistic risk assessment

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International Journal of Sediment Research 2022年第2期37卷 173-187页

作者： Mir Mohammad Ali Saima Rahman Md Saiful Islam Md Refat Jahan Rakib Shaharior Hossen Md Zillur Rahman Tapos Kormoker Abubakr M. Idris Khamphe Phoungthong Environmental Assessment and technology for Hazardous Waste management Research Center Faculty of Environmental Management Prince of Songkla University Quality Control Chemistry Lab Department of Fisheries department of Aquaculture Sher-e-Bangla Agricultural University Research Center for Advanced Materials science (RCAMS) King Khalid University Institute of Disaster management Khulna University of Engineering and Technology department of Soil science Patuakhali Science and Technology University department of Emergency management Patuakhali Science and Technology University department of Urban and Regional Planning Khulna University of Engineering and Technology department of Fisheries and Marine science Noakhali Science and Technology University department of Fisheries science Chonnam National University department of Chemistry College of Science King Khalid University

River water and sediment embody environmental characteristics that give valuable eco-environmental information.Due to rapid industrialization,the aquatic environment of any urban river can be seriously polluted by heavy metals(HMs).The global concern is caused by heavy metal pollution because of its potential harm to aquatic ecosystems and human health.In the Bhairab River,Bangladesh,surface sediment concentrations of globally alarming toxic metals such as arsenic(As),chromium(Cr),cadmium(Cd),and lead(Pb) were measured to determine the ecological and human health risks of the riverine ecosystem.The average As,Cr,Cd,and Pb concentrations in water were 3.55,31.74,1.44,and23.82 μg/L,respectively,and in sediment were 4.13,34.17,1.66,and 25.46 mg/kg,respectively.During the winter,metals in sediment were higher than during the summer.For most sediment samples,the enrichment factor(EF),contamination factor(CF),geoaccumulation index(I),pollution load index(PLI) of As,Cr,Cd,and Pb indicated moderate contamination.The potential ecological risk(PER) in sediment followed the descending order of Cd> As> Pb> Cr.The contamination level of toxic metals implied that the condition is frightening and probably severely affecting the aquatic ecology of this riverine ecosystem.

关键词： Toxic metals Environmental risk Bioaccumulation Ecological risk Bangladesh