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Detecting SARS-CoV-2 BA.2,BA.4,and BA.5 Variants Utilizing a Robust RT-RPA-CRISPR/Cas12a-Based Method-China,2023

China CDC weekly 2023年第26期5卷 584-591页

作者： Meihui Luo Yang Pan Yaqing He Ruhan A Changcheng Wu Baoying Huang Roujian Lu Li Zhao Bo Peng Fei Ye Huijuan Wang Yuda Chen Zhen Li Daitao Zhang wenling Wang Wenjie Tan MHC Key Laboratory of Biosafety National Institute for Viral Disease Control and PreventionChina CDCBeijingChina Beijing Center for Disease Prevention and Control BeijingChina Microbiology Laboratory Shenzhen Center for Disease Control and PreventionShenzhen CityGuangdong ProvinceChina school of Public Health Baotou Medical CollegeBaotou CityInner Mongolia Autonomous RegionChina Collaborative Innovation Centre for Regenerative Medicine and Medical BioResourse Development and Application Co-constructed by the Province and Ministry Guangxi Medical UniversityGuangxi Zhuang Autonomous RegionChina

Introduction:Since 2019,numerous variants of concern for severe acute respiratory syndrome virus 2(SARS-CoV-2)have emerged,leading to significant *** development of novel,highly accurate,and rapid detection techniques for these new SARSCoV-2 variants remains a primary focus in the ongoing efforts to control and prevent the coronavirus disease 2019(COVID-19)***:Reverse transcription-recombinase polymerase amplification combined with the clustered regularly interspaced short palindromic repeatsassociated protein 12a(CRISPR/Cas12a)system was used to validate the detection of the Omicron BA.2,BA.4,and BA.5 variants of ***:Our results demonstrate that the CRISPR/Cas12a assay is capable of effectively detecting the SARS-CoV-2 BA.2,BA.4,and BA.5 variants with a limit of detection of 10,1,and 10 copies/μL,***,our assay successfully differentiated the three SARS-CoV-2 Omicron strains from one ***,we evaluated 46 SARS-CoV-2 positive clinical samples consisting of BA.2(n=20),BA.4(n=6),and BA.5(n=20)variants,and the sensitivity of our assay ranged from 90%to 100%,while the specificity was 100%.Discussion:This research presents a swift and reliable CRISPR-based method that may be employed to track the emergence of novel SARS-CoV-2 variants.

关键词： CRISPR/Cas limit specificity

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Atomically dispersed N-coordinated Fe-Fe dual-sites with enhanced enzyme-like activities

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Nano Research 2022年第2期15卷 959-964页

作者： Lei Jiao Wei Ye Yikun Kang Yu Zhang Weiqing Xu Yu Wu wenling Gu Weiyu Song Yujie Xiong Chengzhou Zhu Key Laboratory of Pesticide and Chemical Biology of Ministry of Education International Joint Research Center for Intelligent Biosensing Technology and HealthCollege of ChemistryCentral China Normal UniversityWuhan 430079China College of Material Chemistry and Chemical EngineeringHangzhou Normal UniversityHangzhou 311121China State Key Laboratory of Heavy Oil Processing China University of PetroleumBeijing 102249China Hefei National Laboratory for Physical Sciences at the Microscale Collaborative Innovation Center of Chemistry for Energy Materials(iChEM)School of Chemistry and Materials Scienceand National Synchrotron Radiation LaboratoryUniversity of Science and Technology of ChinaHefei 230026China

Replacement of enzymes with nanomaterials such as atomically dispersed metal catalysts is one of the most crucial steps in addressing the challenges in *** the breakthroughs of single-atom catalysts in enzyme-mimicking,a fundamental investigation on the development of an instructional strategy is still required for mimicking biatomic/multiatomic active sites in natural enzymes and constructing synergistically enhanced metal atom active ***,Fe_(2)NC catalysts with atomically dispersed Fe-Fe dual-sites supported by the metal-organic frameworks-derived nitrogen-doped carbon are employed as biomimetic catalysts to perform proof-of-concept *** effect of Fe atom number toward typical oxidase(cytochrome C oxidase,NADH oxidase,and ascorbic acid oxidase)and peroxidase(NADH peroxidase and ascorbic acid peroxidase)activities is systematically evaluated by experimental and theoretical investigations.A peroxo-like O_(2) adsorption in Fe_(2)NC nanozymes could accelerate the O-O activation and thus achieve the enhanced enzyme-like *** work achieves the vivid simulation of the enzyme active sites and provides the theoretical basis for the design of high-performance *** a concept application,a colorimetric biosensor for the detection of S^(2-) in tap water is established based on the inhibition of enzyme-like activity of Fe_(2)NC nanozymes.

关键词： nanozymes atomically dispersed dual-metal sites oxidase-like activities single-atom catalysis sensors

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Development and Evaluation of a Universal and Supersensitive NS1-Based Luciferase Immunosorbent Assay to Detect Zika Virus-Specific IgG

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Virologica Sinica 2020年第1期35卷 93-102页

作者： Tianyu Wang Ying Zhan De Wu Zhihai Chen Wei Wu Yao Deng wenling Wang Wenjie Tan Shixing Tang Guangdong Provincial Key Laboratory of Tropical Disease Research Department of EpidemiologySchool of Public HealthSouthern Medical UniversityGuangzhou 510515China NHC Key Laboratory of Biosafety National Institute for Viral Disease Control and PreventionChina CDCBeijing 102206China Key Laboratory for Repository and Application of Pathogenic Microbiology Research Center for Pathogens Detection Technology of Emerging Infectious DiseasesGuangdong Provincial Center for Disease Control and PreventionGuangzhou 511430China The National Clinical Key Department of Infectious Disease Beijing Ditan HospitalCapital Medical UniversityBeijing 100015China

Zika virus(ZIKV) causes rash, moderate fever, conjunctivitis, and arthralgia, and has serious connection with neurological complications;therefore, it is a major threat to public health. A rapid and supersensitive method for detecting anti-ZIKV antibodies in humans and animals is thus urgently required. Here, we report an NS1-based luciferase immunosorbent assay(LISA), developed to detect ZIKV-specific IgG. Fusion proteins including a reporter Nano-luciferase(NLuc) and various fragments of ZIKV NS1 protein were expressed in 293 T cells. LISA was performed using the above cell lysates containing the expressed fusion proteins. Sample panels of humans and animals infected with ZIKV were examined for sensitivity of LISA, relative to those of ZIKV RT-PCR, commercial NS1-based ELISA, and micro-neutralization(MN) *** and potential cross-reactivity were also evaluated using various convalescent serum samples derived from patients infected with dengue virus(DENV), Japanese encephalitis virus(JEV), and hepatitis C virus(HCV). Results indicated the optimal antigenic domain for anti-ZIKV IgG detection was located within 172–352 amino acids(aa) of ZIKV NS1 protein. NS1-based LISA performs better than commercial ELISA in anti-ZIKV Ig G detection. LISA was shown to be at least fourfold more sensitive than commercial ELISA, and could detect anti-ZIKV Ig G in various animal hosts without the need of species-specific labeled antibody. This novel assay is potentially useful for the rapid and sensitive detection of anti-ZIKV IgG in human and animal samples.

关键词： Zika virus(ZIKV) NS1 Luciferase immunosorbent assay IgG Detection

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The mechanisms of sorafenib resistance in hepatocellular carcinoma:theoretical basis and therapeutic aspects

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Signal Transduction and Targeted Therapy 2020年第1期5卷 1713-1727页

作者： Weiwei Tang Ziyi Chen wenling Zhang Ye Cheng Betty Zhang Fan Wu Qian Wang Shouju Wang Dawei Rong F.P.Reiter E.N.De Toni Xuehao Wang Department of General Surgery Nanjing First HospitalNanjing Medical UniversityNanjingChina Hepatobiliary/Liver Transplantation Center The First Affiliated Hospital of Nanjing Medical UniversityKey Laboratory of Living Donor TransplantationChinese Academy of Medical SciencesNanjingChina Department of Gastroenterology The First Affiliated Hospital of Nanjing Medical UniversityNanjingChina Michael G.DeGroote school of Medicine McMaster UniversityHamiltonONCanada Department of Radiology The First Affiliated Hospital of Nanjing Medical UniversityNanjingChina Department of Medicine II University HospitalLMUMunichGermany Liver Center Munich University HospitalLMUMunichGermany

Sorafenib is a multikinase inhibitor capable of facilitating apoptosis,mitigating angiogenesis and suppressing tumor cell *** late-stage hepatocellular carcinoma(HCC),sorafenib is currently an effective first-line ***,the development of drug resistance to sorafenib is becoming increasingly *** study aims to identify factors contributing to resistance and ways to mitigate *** studies have shown that epigenetics,transport processes,regulated cell death,and the tumor microenvironment are involved in the development of sorafenib resistance in HCC and subsequent HCC *** study summarizes discoveries achieved recently in terms of the principles of sorafenib resistance and outlines approaches suitable for improving therapeutic outcomes for HCC patients.

关键词： sorafenib therapeutic hepatocellular

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Carboxyl-functionalized Nanocellulose Reinforced Nanocomposite Proton Exchange Membrane

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Chemical Research in Chinese Universities 2019年第4期35卷 735-741页

作者： YANG Xue WEI Yingcong CHU Xuefeng ZHAO Qi YAN wenling DONG Chenjun LIU Baijun SUN Zhaoyan HU Wei ZHANG Niaona College of Chemical Engineering Changchun University of TechnologyChangchun 130012P. R. China State Key Laboratory of Photocatalysis on Energy and Environment College of Chemistry Fuzhou University Fuzhou 350002 P. R. China school of Electrical and Electronic Information Engineering Jilin Jianzhu University Changchun 13011& P. R. China College of Chemistry Jilin UniversityChangchun 130012P. R. China State Key Laboratory of Polymer Physics and Chemistry Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 P. R. China

Nanocellulose(NCC) was prepared through the acid hydrolysis of microcellulose(MCC) and was reacted with maleic anliydride to obtain carboxyl-functionized nanocellulose(MA-NCCs). The presence of .OH and .SO3H on the surface of rod-like MA-NCC was confirmed by Fourier transfonn infrared spectrometry(FTIR). Sulfonated poly(aryl ether ether ketone ketone)(Ph-SPEEKK) was synthesized with bis(4-fluorophenyl-methanone) and 2-phenylhydroquinone as monomer. MA-NCC/Ph-SPEEKK nanocomposite membranes with different MA-NCCs content were prepared, and their properties were characterized. Compared with Ph-SPEEKK, MA-NCC/Ph-SPEEKK nanocomposite membrane showed better mechanical and thermal properties and higher proton conductivity. The proton conductivity of the composite membrane with 4%(mass fraction) MA-NCCs under 80℃ was 0.095 S/cm. And its tensile strength reached 30.3 MPa, which was 21.2% higher than that of Ph-SPEEKK pure polymer membrane.

关键词： Sulfonated poly(arylether etlier ketone ketone) Carboxylation Nanocellulose Proton exchange membrane

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Morphological and genetic comparison of two strains of a Prorocentrum species isolated from Zhejiang coastal water of China and Masan Bay of Korea

Morphological and genetic comparison of two strains of a Pro...

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中国藻类学会第八次会员大会暨第十六次学术讨论会

作者： GOEBEL Jeanette JEONG Hae Jin Landesamt für Landwirtschaft Umwelt und lndliche RumeHamburger Chaussee 2524220 FlintbekGermany school of Earth and Environmental Science College of Natural SciencesSeoul National UniversityKorea

＜正＞In this paper,we examined the detailed morphology of two strains of Prorocentrum isolated from the coastal waters of Zhejiang（wenling area）,China and Masan Bay of Korea.A taxonomic comparison was made among strains on the basis of morphological and molecular *** cellular dimensions of the Chinese wenling strain （LAMB090508） and Korean strain（PDKS0206） were similar and the cells of both strains were of asymmetric and elongated *** posterior end of most cells was rounded. Megacytic zones of senescent cells were broader with dense tiny *** roundish nucleus was positioned in the posterior part of the cell.A few irregular shaped chloroplasts were distributed within the *** nucleotide similarity of the two strains,determined from the ITS rDNA sequences,was 99.83%.The comparative results suggested that both strains isolated from China and Korea were the identical species, Prorocentrum donghaiense Lu.

关键词： Prorocentrum morphology nrDNA ITS coastal waters of China Korea

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Single-atom nanozymes with axial ligand-induced self-adaptive conformation in alkaline medium boost chemiluminescence

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Science China Chemistry 2023年第3期66卷 904-912页

作者： Zhen Luo Liliang Tian Hengjia Wang Zhichao Wu Xin Luo Xiaosi Wang Lei Jiao Xiaoqian Wei Ying Qin Lirong Zheng Liuyong Hu wenling Gu Le Shi Chengzhou Zhu Key Laboratory of Pesticides and Chemical Biology of Ministry of Education International Joint Research Center for Intelligent Biosensing Technology and HealthCollege of ChemistryCentral China Normal UniversityWuhan 430079China State Key Laboratory of Electrical Insulation and Power Equipment Centre of Nanomaterials for Renewable EnergySchool of Electrical EngineeringXi’an Jiaotong UniversityXi’an 710049China Beijing Synchrotron Radiation Facility Institute of High Energy PhysicsChinese Academy of SciencesBeijing 100049China Hubei Key Laboratory of Plasma Chemistry and Advanced Materials Hubei Engineering Technology Research Center of Optoelectronic and New Energy MaterialsWuhan Institute of TechnologyWuhan 430205China Fujian Shuikou Power Generation Group Corp. Fuzhou 350000China

Mimicking the structure of natural enzymes for designing advanced alternatives provides great opportunities to address the bottleneck of enzyme-involved chemiluminescence(CL). Herein, according to theoretical calculations, we found that an endogenous axial ligand of M-N-C single-atom nanozymes(SAzymes), originating from OH-spontaneously bonding to the metal center in an alkaline medium, can self-adaptively change its strength to facilitate intermediate steps. Furthermore, the lowest energy barrier of the rate-determining step and the strongest affinity and fastest electron transfer with luminol anion endow CoN-C with the highest peroxidase-like activity. Guided by the theoretical calculations, a series of M-N-C SAzymes(M=Fe, Co,Ni) were synthesized to boost CL, where Co-N-C SAzymes with superior catalytic activity and high selective generation of O_(2)·- were validated. As a proof-of-concept, Co-N-C SAzymes were employed for sensitive detection of acetylcholinesterase and organophosphorus pesticide.

关键词： single-atom nanozymes chemiluminescence hydrogen peroxide activation biosensing

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RXRα agonist bexarotene attenuates radiation-induced skin injury by relieving oxidative stress

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Radiation Medicine and Protection 2022年第2期3卷 56-63页

作者： Sheng Jiang Weichao Cai Jianhui Chen wenling Tu Yulan Liu Lixin Gong Yahui Feng Wei Mo Tao Yan Shuyu Zhang Daojiang Yu The Second Affiliated Hospital of Chengdu Medical College China National Nuclear Corporation No.416 HospitalChengdu 610051China Department of Plastic Surgery Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical UniversityTaizhou 317000China West China Second University Hospital West China School of Basic Medical Sciences&Forensic MedicineSichuan UniversityChengdu 610041China State Key Lab of Radiation Medicine and Protection School of Radiation Medicine and ProtectionMedical College of Soochow UniversitySuzhou 215123China NHC Key Laboratory of Nuclear Technology Medical Transformation(Mianyang Central Hospital) Mianyang 621099China

Objective: To investigate the protective effect of bexarotene in radiation-induced skin injury and elucidate underlying ***: Irradiated cellular and animal models were established using an X-ray linear accelerator. Cell viabilityand apoptosis were evaluated by CCK8 and flow cytometry. In vivo protective effect of bexarotene was measuredin irradiated SD rats. The antioxidant capacity of bexarotene was validated by DCF-DA method. The signalingpathways involved in bexarotene-mediated skin repair were enriched by RNA ***: Bexarotene could significantly restore the proliferation and inhibit the apoptosis of WS1 cells with radiation damage (P < 0.05). Moreover, bexarotene could effectively shorten the process of skin damage andpromote skin repair in a rat model of radiation-induced skin injury (P < 0.05). Mechanistically, bexaroteneeffectively reduced the expression of RXRα (P < 0.05), thus leading to an early decrease in reactive oxygen species(ROS) after radiation exposure. Furthermore, a transcriptome analysis indicated that bexarotene-mediated recovery of radiation damage involves redox signaling, immune regulation, lipid metabolism and ***: Bexarotene is a promising therapeutic agent in the treatment of radiation-induced skin injury.

关键词： Radiation Skin injury Oxidative stress RXRα Bexarotene

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A novel luciferase immunosorbent assay performs better than a commercial enzyme-linked immunosorbent assay to detect MERS-CoV specific IgG in humans and animals

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Biosafety and Health 2019年第3期1卷 134-143页

作者： wenling Wang Tianyu Wang Yao Deng Peihua Niu Ruhan A Jincun Zhao Malik Peiris Shixing Tang Wenjie Tan MHC Key Laboratory of Biosafety National Institute for Viral Disease Control and PreventionChina CDCBeijing 102206China Guangdong Provincial Key Laboratory of Tropical Disease Research Department of EpidemiologySchool of Public HealthSouthern Medical UniversityGuangzhou 510515China State Key Laboratory of Respiratory Disease National Clinical Research Center for Respiratory DiseaseGuangzhou Institute of Respiratory DiseaseThe first Affiliated Hospital of Guangzhou Medical UniversityGuangzhou 510182China school of Public Health Li Ka Shing Faculty of MedicineThe University of Hong KongPokfulamHong Kong Special Administrative Region Center for Biosafety Mega-science Chinese Academy of SciencesBeijing 100101China

The Middle East respiratory syndrome(MERS)is a lethal zoonosis caused by MERS coronavirus(MERS-CoV)and poses a significant threat to public health ***,a rapid,sensitive,and specific serologic test for detecting anti-MERS-CoV antibodies in both humans and animals is urgently needed for the successful management of this ***,we evaluated various novel luciferase immunosorbent assays(LISA)based on nucleocapsid protein(NP)as well as fragments derived from spike protein(S)including subunit 1(S1),N terminal domain(NTD),receptorbinding domain(RBD)and subunit 2(S2)of S for the detection of MERS-CoV-specific *** proteins,including nanoluciferase(NLuc)and various fragments derived from the NP or S protein of MERS-CoV,were expressed in human embryonic kidney 293 T *** that detected anti-MERS-CoV IgG were further developed using cell lysates expressing various fusion *** of human or animal samples infected with MERS-CoV were used to analyze the sensitivity and specificity of various LISAs in reference to a MERS-CoV RT-PCR,commercial S1-based ELISA,and pseudovirus particle neutralization test(ppNT).Our results showed that the S1-,RBD-,and NP-LISAs were more sensitive than the NTD-and S2-LISAs for the detection of anti-MERS-CoV ***,the S1-,RBD-,and NP-LISAs were more sensitive(by at least 16-fold)than the commercially available ***,the S1-,RBD-,and NPLISA specifically recognized anti-MERS-CoV IgG and did not cross-react with samples derived from other human CoV(OC43,229E,HKU1,NL63)-infected *** importantly,these LISAs proved their applicability and reliability for detecting anti-MERS-CoV IgG in samples from camels,monkeys,and mice,among which the RBD-LISA exhibited excellent *** results of this study suggest that the novel MERS-CoV RBD-and S1-LISAs are highly effective platforms for the rapid and sensitive detection of anti-MERS-CoV IgG in human and animal *** assays have

关键词： Luciferase immunosorbent assay(LISA) MERS-CoV Serological IgG detection Samples of humans and animals

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Long non-coding RNAs in cancer

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Science China(Life Sciences) 2012年第12期55卷 1120-1124页

作者： GONG ZhaoJian ZHANG ShanShan ZHANG wenling HUANG HongBin LI Qiao DENG Hao MA Jian ZHOU Ming XIANG Juan Juan WU MingHua LI XiaYu XIONG Wei LI XiaoLing LI Yong ZENG ZhaoYang LI GuiYuan Hunan Key Laboratory of Nonresolving Inflammation and Cancer Disease Genome Research Center the Third Xiangya Hospital Central South University Changsha 410013 China Key Laboratory of Carcinogenesis of Ministry of Health Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education Cancer Research Institute Central South University Changsha 410078 China Department of Stomatology the Second Xiangya Hospital Central South University Changsha 410011 China Department of Stomatology Xiangya Hospital Central South University Changsha 410008 China Key Laboratory of lnformation System Engineering National University of Defense Technology Changsha 410073 China Department of Biochemistry and Molecular Biology Center for Genetics and Molecular Medicine School of Medicine University of Louisville Louisville KY 40202 USA

Long non-coding RNAs （lncRNAs） are a group of RNA transcripts that exceed 200 nt in length, yet lack significant open reading frames （ORFs） [1-4]. In contrast to small non-coding RNAs （ncRNAs）, such as microRNAs （miR- NAs） [4-23], small interfering RNAs （siRNAs） [24-31] and transfer RNAs （tRNAs） [32-34], there are thousands of IncRNA genes discovered during the past three years in the human genome and most of their functions remain elusive. The long nucleotide chain of lncRNAs can either form a complex spatial structure and interact with protein factors, or provide a large segment for the concurrent binding of many molecules that collectively participate in genomic imprinting, X-chromosome silencing, chromosome modifi- cation, intranuclear transport, transcriptional activation and interference, thereby regulating cell growth, differentiation, development, senescence and death [35].

关键词：非编码RNA tRNA基因 siRNAs 癌症小分子RNA 转录激活开放阅读框转移RNA

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