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Two antibodies show broad,synergistic neutralization against SARS-CoV-2 variants by inducing conformational change within the RBD

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Protein & Cell 2024年第2期15卷 121-134页

作者： Hui Sun Tingting Deng Yali Zhang Yanling Lin Yanan Jiang Yichao Jiang Yang Huang Shuo Song Lingyan Cui Tingting Li Hualong Xiong Miaolin Lan Liqin Liu Yu Li Qianjiao Fang Kunyu Yu Wenling Jiang Lizhi Zhou Yuqiong Que Tianying Zhang Quan Yuan Tong Cheng Zheng Zhang Hai Yu Jun Zhang Wenxin Luo Shaowei Li Qingbing Zheng Ying Gu Ningshao Xia State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics School of Public HealthSchool of Life SciencesXiamen UniversityXiamen 361102China National Institute of Diagnostics and Vaccine Development in Infectious Diseases School of Public HealthSchool of Life SciencesXiamen UniversityXiamen 361102China State Key Laboratory of Vaccines for Infectious Diseases School of Public HealthSchool of Life SciencesXiamen UniversityXiamen 361102China Xiang An Biomedicine Laboratory Xiamen 361102China Institute for Hepatology National Clinical Research Center for Infectious DiseaseShenzhen Third People's HospitalSchool of MedicineSouthem University of Science and TechnologyShenzhen 518112China The Second Affliated Hospital School of MedicineSouthern University of Science and TechnologyShenzhen 518112China Research Unit of Frontier Technology of Structural Vaccinology Chinese Academy of Medical SciencesXiamen 361102China

Continual evolution of the severe acute respiratory syndrome coronavirus(SARS-CoV-2)virus has allowed for its gradual evasion of neutralizing antibodies(nAbs)produced in response to natural infection or *** rapid nature of these changes has incited a need for the development of superior broad nAbs(bnAbs)and/or the rational design of an antibody cocktail that can protect against the mutated virus ***,we report two angiotensin-converting enzyme 2 competing nAbs—8H12 and 3E2—with synergistic neutralization but evaded by some Omicron ***-electron microscopy reveals the two nAbs synergistic neutralizing virus through a rigorous pairing permitted by rearrangement of the 472-489 loop in the receptor-binding domain to avoid steric *** antibodies based on these two nAbs tremendously extend the neutralizing breadth and restore neutralization against recent variants including currently dominant ***,these findings expand our understanding of the potential strategies for the neutralization of SARS-CoV-2 variants toward the design of broad-acting antibody therapeutics and vaccines.

关键词： SARS-CoV-2 broad neutralizing antibody rearrangement synergistic neutralization

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Evolution and Determinants of Population Agglomeration in Less Developed Metropolitan Areas:A Case Study of the Taiyuan Metropolitan Area,China

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Chinese Geographical Science 2024年第4期34卷 739-751页

作者： QIN Zhiqin LIANG Ye AN Shuwei DOU Yongjing school of Economics and Management Shanxi UniversityTaiyuan 030031China school of Urban Economics and public Affairs Capital University of Economics and BusinessBeijing 100070China school of Geography Science Taiyuan Normal UniversityJinzhong 030619China

It is of importance to enhance the urban areas'capacity for population aggregation in underdeveloped regions,aiming to rectify the imbalanced and insufficient pattern of economic development in *** the Taiyuan Metropolitan Area(TMA)in central China as a case study,this paper examines the evolutionary process and characteristics of population agglomeration from 2000 to 2020,and identifies factors associated with agglomeration and their spatial *** findings indicated that:1)against the background of sustained population shrinkage in the provincial area,the TMA showed a demographic trend of steady increase,albeit with a decelerated growth *** the metropolitan area,urban population size continued to grow rapidly,whereas the rural areas endured sustained *** in city size continued to widen,and the polarization of concentrated population in the core cities kept increasing.2)Agglomerations in both secondary and service industries had significant positive effects on local population agglomeration,with the former effect being *** economic development,government fiscal expenditure,and financial advancement all contributed to facilitating local population *** a spatial spillover perspective,service agglomeration and financial development promoted population agglomeration in surrounding ***,fiscal expenditure inhibited such *** for industrial agglomeration and regional economic development,their spatial spillover effects were *** results obtained reveal several policy implications aimed at enhancing the population agglomeration capacity of the metropolitan area in underdeveloped regions during the new era.

关键词： population agglomeration population shrinkage spatial spillover effects Taiyuan Metropolitan Area(TMA) China

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Demethylase-assisted site-specific detection of N^(1)-methyladenosine in RNA

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Chinese Chemical Letters 2024年第5期35卷 397-401页

作者： Jun Xiong Ke-Ke Chen Neng-Bin Xie Wei Chen Wen-Xuan Shao Tong-Tong Ji Si-Yu Yu Yu-Qi Feng Bi-Feng Yuan College of Chemistry and Molecular Sciences Research Center of Public HealthRenmin Hospital of Wuhan UniversitySchool of Public HealthWuhan UniversityWuhan 430071China Department of Laboratory Medicine Zhongnan Hospital of Wuhan UniversityWuhan UniversityWuhan 430071China

The dynamic RNA modifications have been viewed as new posttranscriptional regulator in modulating gene expression as well as in a broad range of physiological processes.N^(1)-methyladenosine(m^(1)A)is one of the most prevalent modifications existing in multiple types of ***-depth investigation of the functions of m^(1)A requires the site-specific assessment of m^(1)A stoichiometry in ***,we established a demethylase-assisted method(DA-m^(1)A)for the site-specific detection and quantification of m^(1)A in RNA.N^(1)-methyl group in m^(1)A could result in the stalling of reverse transcription at m^(1)A site,thus producing the truncated *** AlkB is a demethylase that can demethylate m^(1)A to produce adenine in RNA,thus generating full-length cDNA from AlkB-treated *** of the produced amounts of full-length cDNA by quantitative real-time PCR can achieve the site-specific detection and quantification of m^(1)A in *** the DA-m^(1)A method,we examined and successfully confirmed the previously well-characterized m^(1)A sites in various types of RNAs with low false positive *** addition,we found that the level of m^(1)A was significantly decreased at the bromodomain containing 2(BRD2)mRNA position 1674 and CST telomere replication complex component 1(CTC1)mRNA position 5643 in human hepatocellular carcinoma *** results suggest that these two m^(1)A sites in mRNA may be involved in liver *** together,the DA-m^(1)A method is simple and enables the rapid,cost-effective,and site-specific detection and quantification of m^(1)A in RNA,which provides a valuable tool to decipher the functions of m^(1)A in human diseases.

关键词： N^(1)-methyladenosine RNA modification AlkB demethylase RNA epigenetics Quantitative real-time PCR Hepatocellular carcinoma

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Higher ambient temperatures may worsen obstructive sleep apnea:A nationwide smartwatch-based analysis of 6.2 million person-days

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Science Bulletin 2024年第13期69卷 2114-2121页

作者： Anni Li Qingli Zhang Yuan Yao Xinlei Zhu Cong Liu Yutao Guo Haidong Kan Renjie Chen school of public Health Key Lab of Public Health Safety of the Ministry of Education and NHC Key Lab of Health Technology AssessmentFudan UniversityShanghai 200032China Ministry of Education-Shanghai Key Laboratory of Children’s Environmental Health Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200092China Department of Pulmonary Vessel and Thrombotic Disease Sixth Medical CenterChinese PLA General HospitalBeijing 100048China Institute for Hospital Management Research Chinese PLA General HospitalBeijing 100048China Children’s Hospital of Fudan University National Children’s Medical CenterShanghai 201102China school of public Health Hengyang Medical SchoolUniversity of South ChinaHengyang 421001China

Obstructive sleep apnea(OSA)is a serious type of sleep disorder that can lead to cardiometabolic and neurocognitive *** utilized smart device-based photoplethysmography technology to collect sleep data from the Chinese population from 2019 to *** lag nonlinear models combined with a generalized nonlinear model or a linear mixed effects model were used to investigate the shortterm associations between daily temperature and indicators of OSA *** included a total of 6,232,056 d of sleep monitoring data from 51,842 participants with moderate to severe risk of OSA from 313 Chinese *** relationships between ambient temperature and OSA exacerbation,apneahypopnea index(AHI),and minimum oxygen saturation(MinSpO_(2))were almost linear and present only on the same *** temperatures were associated with a greater risk of OSA exacerbation,with an 8.4%(95%confidence interval(CI):7.6%–9.3%)increase per 10℃increase in temperature.A 10℃increase in daily temperature corresponded to an AHI increase of 0.70 events/h(95%CI:0.65–0.76)and a MinSpO_(2)decrease of 0.18%(95%CI:0.16%–0.19%).Exposure to elevated temperatures during the night can also lead to adverse *** effects of higher temperatures on OSA severity were stronger among men,participants with a body mass index24 kg/m2,those aged 45 years and older,individuals with a history of hypertension and diabetes,and during the cold *** large-scale,nationwide,longitudinal study provides robust evidence suggesting that higher ambient temperatures may immediately worsen OSA.

关键词： Obstructive sleep apnea Temperature Apnea-hypopnea index Oxygen saturation Smart device

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Microbiome-driven anticancer therapy:A step forward from natural products

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mLife 2024年第2期3卷 219-230页

作者： Yunxuan Guan Di Wu Hui Wang Ning-Ning Liu State Key Laboratory of Systems Medicine for Cancer Center for Single‐Cell OmicsSchool of Public HealthShanghai Jiao Tong University School of MedicineShanghaiChina

Human microbiomes,considered as a new emerging and enabling cancer hallmark,are increasingly recognized as critical effectors in cancer development and *** of microbiome revitalizing anticancer therapy from natural products shows promise toward improving cancer ***,we summarize our current understanding of the human microbiome‐driven molecular mechanisms impacting cancer progression and anticancer *** highlight the potential translational and clinical implications of natural products for cancer prevention and treatment by developing targeted therapeutic strategies as adjuvants for chemotherapy and immunotherapy against *** challenges and opportunities for future investigations using modulation of the microbiome for cancer treatment are further discussed in this review.

关键词： anticancer cancer chemotherapy immunotherapy microbiota

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Interregional Value-Added Tax in the Era of E-Commerce: Tax Policy Design Based on Big Data from Online Retailing

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Journal of Social Computing 2024年第1期5卷 46-57页

作者： Yu Chen Luwei Xiang Hu Yang school of public Finance and Taxation Central University of Finance and EconomicsBeijing 100008China school of Management and Economics The Chinese University of Hong KongShenzhen 518172China school of Information Central University of Finance and EconomicsBeijing 100008China

The value-added tax(VAT)in China is levied and allocated based on the origin *** the background of the increasing substitution of online retail for traditional offline retail,this mechanism will exacerbate the disparity of regional tax revenue,and intensify tax competition among local ***,reconsidering the allocation mechanism of value-added tax in China can be an important policy decision,and it is influenced by various economic and social ***,we utilize large-scale retail transaction data from an e-commerce platform to measure regional disparities in retail and consumption among different regions and then reveals present tax policy results in revenue imbalance in different ***,we establish a model based on game theory to illustrate how the origin principle leads to fierce tax competition among ***,by establishing and solving tax allocation models between local governments and the central government,this study simulates and calculates the degree of revenue imbalance under different scenarios and attempts to propose policy *** results indicate that implementing the destination principle will reduce regional tax ***,adjusting the allocation ratio between the central government and local governments based on city levels is advantageous for further reducing regional tax revenue disparities.

关键词： value-added tax e-commerce destination principle origin principle

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Downregulation of Serum PTEN Expression in Mercury-Exposed Population and PI3K/AKT Pathway-Induced Inflammation

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Biomedical and Environmental Sciences 2024年第4期37卷 354-366页

作者： MEI Peng DING En Min YIN Hao Yang DING Xue Xue WANG Huan WANG Jian Feng HAN Lei ZHANG Heng Dong ZHU Bao Li school of public Health Nanjing Medical UniversityNanjing 210000JiangsuChina Institute of Occupational Disease Prevention Jiangsu Province Center for Disease Prevention and ControlNanjing 21000JiangsuChina Suzhou Center for Disease Prevention and Control Suzhou 215004JiangsuChina Center for Global Health School of Public HealthNanjing Medical UniversityNanjing 210000JiangsuChina

Objective This study investigated the impact of occupational mercury(Hg) exposure on human gene transcription and expression, and its potential biological *** Differentially expressed genes related to Hg exposure were identified and validated using gene expression microarray analysis and extended validation. Hg-exposed cell models and PTEN lowexpression models were established in vitro using 293T cells. PTEN gene expression was assessed using qRT-PCR, and Western blotting was used to measure PTEN, AKT, and PI3K protein levels. IL-6 expression was determined by *** Combined findings from gene expression microarray analysis, bioinformatics, and population expansion validation indicated significant downregulation of the PTEN gene in the high-concentration Hg exposure group. In the Hg-exposed cell model(25 and 10 μmol/L), a significant decrease in PTEN expression was observed, accompanied by a significant increase in PI3K, AKT, and IL-6 ***, a low-expression cell model demonstrated that PTEN gene knockdown led to a significant decrease in PTEN protein expression and a substantial increase in PI3K, AKT, and IL-6 *** This is the first study to report that Hg exposure downregulates the PTEN gene, activates the PI3K/AKT regulatory pathway, and increases the expression of inflammatory factors, ultimately resulting in kidney inflammation.

关键词： PTEN Occupational mercury exposure Occupational health PI3K/AKT pathway 293T cell IL-6

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Significance of school greenspaces in preventing childhood myopia

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The Innovation 2024年第1期5卷 11-12页

作者： Bo-Yi Yang Department of Occupational and Environmental Health School of Public HealthSun Yat-Sen UniversityGuangzhou 510080China

THE BURDEN OF MYOPIA IN CHINESE CHILDREN Childhood myopia has emerged as a critical and underestimated public health issue globally,particularly in China and east Asian *** prevalence of myopia in Chinese children and adolescents is alarmingly high,with rates of 72%and 81%among middle school(ages 11–13 years)and high school(ages 14–17 years)students,respectively,resulting in an overall prevalence of approximately 54%.

关键词： globally myopia school

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Associations of heavy metal mixtures with blood pressure among *** in NHANES 2017-2018 by four statistical models

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Chinese Medical Journal 2024年第5期137卷 628-630页

作者： Luli Wu Shixuan Zhang Junrou Zhang Ye Xin Piye Niu Jie Li Department of Occupational and Environmental Health School of Public HealthCapital Medical UniversityBeijing 100069China Beijing Key Laboratory of Environmental Toxicology School of Public HealthCapital Medical UniversityBeijing 100069China Department of Nutrition Beijing Chaoyang HospitalCapital Medical UniversityBeijing 100020China

To the Editor:Hypertension is one of the leading risk factors for cardiovascular disease and affects approximately 30%of the adult population.^([1,2])Environmental heavy metal pollution is an important public health *** metals may increase the risk of hypertension or other cardiovascular diseases,even beyond the influence of conventional behavioral risk factors.^([3])Meanwhile certain essential trace elements,such as selenium,zinc,and iron,are involved in various enzyme reactions directly related to blood pressure regulation and are beneficial to the human body within a specific range.^([4])The analysis of health outcomes specific to metal mixtures is challenging by nonlinear relationships and interactions between toxic metals and essential trace elements.^([5])The development of innovative statistical methods for analyzing mixtures has greatly facilitated the exploration of the health effects of multiple pollutant exposures,such as Bayesian kernel machine regression(BKMR)and quantile g-computation(QG-C).

关键词： cardiovascular diseases mixtures

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Prevalence of Cannabis Use around the World:A Systematic Review and Meta-Analysis,2000-2024

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China CDC weekly 2024年第25期6卷 597-604,I0013,I0014页

作者： Qianning Wang Zhen Qin Xing Xing He Zhu Zhongwei Jia National Institute of Health Data Science Center for Drug Abuse Prevention and ControlPeking UniversityBeijingChina school of public Health Peking UniversityBeijingChina

This study aims to perform a systematic review and meta-analysis on the global prevalence of cannabis use to inform drug prevention strategies,policy-making,and resource *** study initially screened 177,843 studies published between January 1,2000,and January 15,2024,using peer-reviewed databases including Web of Science,PubMed,Scopus,Embase,and Cochrane ***,595 studies were identified for data extraction,and 39 of these were selected as country-representative *** among the selected studies was assessed using the chi-squared test and I2 statistic,while sensitivity analysis was conducted to evaluate the robustness of the *** prevalence of cannabis use varied between 0.42%and 43.90%across 33 European countries,1.40%to 38.12%across 15 North and South American countries,0.30%to 19.10%across 16 Asian countries,and 1.30%to 48.70%across 18 Oceania and African *** pooled prevalence of cannabis use was 12.0%[95%confidence interval(CI):10.0,14.3]in countries where cannabis is legalized,compared to 5.4%(95%CI:4.3,6.9)in non-legalized *** findings indicate that the prevalence of cannabis use has disproportionately increased in most countries with the implementation of medical or recreational cannabis legalization policies and relevant geographic *** efforts are needed to monitor newly cannabis-legalized countries and prevent initial use.

关键词： prevention legal initially

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