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Neural Regeneration Research 2014年第22期9卷 2013-2018页

作者： Xiaoge Zhang Tian Tang Zhihe Zhao Leilei Zheng Yin Ding State Key Laboratory of Military Stomatology Department of OrthodonticsSchool of Stomatologythe Fourth Military Medical University State Key Laboratory of Oral Diseases Department of OrthodonticsWest China Hospital of StomatologySichuan University Department of orthodontics the Affiliated Hospital of StomatologyChongqing Medical University

This study analyzed 85 articles indexed by the Web of Science concerning nerve regeneration and osseoperception during tooth loss repair. Using the Web of Science database and Citespace Ⅲ software, a document co-citation network map was drawn by document co-citation analysis and word frequency analysis methods. Combined with emergent node secondary literature retrieval, subject headings with apparent changing word frequency trends were retrieved so as to identify research frontiers and development trends. Research frontiers and hotspots for neuronal calcium sensor protein were quantitatively explored to forecast future research developments in nerve regeneration and osseoperception during repair of tooth loss.

关键词： nerve regeneration tooth nerve injury peripheral nerve injury nerve repair osseoperception neuroprotection scientific map Web of Science Citespace neural regeneration

Effects of orthodontic load on the periodontium of autogenously transplanted teeth in beagle dogs

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第11期14卷 1025-1032页

作者： Lu LU Hui-fang SUN Han XUE Jing GUO Yang-xi CHEN Department of orthodontics Stomatology HospitalSchool of StomatologyShandong University Shandong Provincial Key Laboratory of Oral Biomedicine Shandong University Department of orthodontics West China College of StomatologySichuan University

Objective:To observe the periodontal healing of autogenously transplanted teeth loaded orthodontically after autotransplantation in Beagle ***:Forty-eight teeth were autogenously transplanted,24 of which were loaded postoperatively with orthodontic force at different time points and for different *** healing was evaluated by probing pocket depth(PPD),the expression of relevant proteins,and histomorphometric ***:The dental pockets of loaded and non-loaded teeth were both much deeper after the first postoperative week than before transplantation(P<0.05).Later,the PPD,which was measured after postoperative weeks 1,3,5,9 and 13,gradually became *** expressions of alkaline phosphatase(ALP)and basic fibroblast growth factor(bFGF)were higher in loaded teeth than in non-loaded teeth(P<0.05),and in groups subjected to two weeks duration of loading than in other groups at the same load time point(P<0.05).For the same load duration,the expressions of ALP and bFGF in teeth loaded after postoperative week 4 were higher than those of other treatments(P<0.05).According to histomorphometric analyses,an orthodontic force on transplanted teeth applied after postoperative weeks 4 or 8 for two weeks duration should be favorable for periodontal ***:It is advisable to apply an appropriate magnitude of force on autotransplanted teeth,such as orthodontic force,at appropriate time points and for a suitable duration,to achieve the optimal clinical prognosis following autogenous tooth *** results may serve as a basis for subsequent studies in humans so as to make clinical improvements.

关键词： Autogenous tooth transplantation Periodontium healing Orthodontic load

Characterization of the osteogenic potential of mesenchymal stem cells from human periodontal ligament based on cell surface markers

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International Journal of Oral Science 2015年第4期7卷 213-219页

作者： Ruth Alvarez Hye-Lim Lee Cun-Yu Wang Christine Hong Division of Oral Biology and Medicine School of DentistryUniversity of California at Los Angeles Section of orthodontics School of DentistryUniversity of California at Los Angeles

Mesenchymal stem cell （MSC）-mediated therapy has been shown to be clinically effective in regenerating tissue defects. For improved regenerative therapy, it is critical to isolate homogenous populations of MSCs with high capacity to differentiate into appropriate tissues. The utilization of stem cell surface antigens provides a means to identify MSCs from various tissues. However, few surface markers that consistently isolate highly regenerative MSCs have been validated, making it challenging for routine clinical applications and making it all the more imperative to identify reliable surface markers. In this study, we used three surface marker combinations： CD51/CD140a, CD271, and STRO-1/CD146 for the isolation of homogenous populations of dental mesenchymal stem cells （DMSCs） from heterogeneous periodontal ligament cells （PDLCs）. Fluorescence-activated cell sorting analysis revealed that 24% of PDLCs were CD51＋/CD140a＋, 0.8% were CD271＋, and 2.4% were STRO-1＋/CD146＋. Sorted cell populations were further assessed for their multipotent properties by inducing osteogenic and chondrogenic differentiation. All three subsets of isolated DMSCs exhibited differentiation capacity into osteogenic and chondrogenic lineages but with varying degrees. CD271＋ DMSCs demonstrated the greatest osteogenic potential with strong induction of osteogenic markers such as DLX5, RUNX2, and BGLAP. Our study provides evidence that surface marker combinations used in this study are sufficient markers for the isolation of DMSCs from PDLCs. These results provide important insight into using specific surface markers for identifying homogenous populations of DMSCs for their improved utilization in regenerative medicine.

关键词： cell surface markers dental mesenchymal stem cells periodontal ligament

Comparative Characterization of Maxillary Expansion and Alternate Maxillary Expansions and Constrictions in Rats

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2014年第6期34卷 935-941页

作者：冯光耀邹冰爽曾祥龙 Department of First Dental Center Peking University School and Hospital of Stomatology Department of orthodontics Peking University School and Hospital of Stomatology

The purpose of this study was to evaluate whether the cranial and circumaxillary sutures react differently to maxillary expansion （ME） and alternate maxillary expansions and constrictions （Alt-MEC） in a rat model. Twenty-two male Sprague-Dawley rats （6 weeks old） were used and divided into three groups. In ME group （n=9）, an expander was activated for 5 days. In Alt-MEC group （9 animals）, an al- ternate expansion and constriction protocol （5-day expansion and 5-day constriction for one cycle） was conducted for 2.5 cycles （25 days total）. The control group comprised 4 animals with no appliances used, each of two sacrificed on day 5 and day 25 respectively. Midpalatal suture expansion or constriction levels were assessed qualitatively and quantitatively by bite-wing X-rays and cast models. Distances between two central incisors and two maxillary first molars were measured on cast models after each activation. Circumaxillary sutures （midpalatal, maxillopalatine, premaxillary, zygomaticotemporal and frontonasal suture） in each group were characterized histologically. Results showed that midpalatal suture was wid- ened and restored after each expansion and constriction. At the end of activation, the widths between both central incisors and first molars in Alt-MEC group were significantly larger than those in ME group （P〈0.05）. Histologically, all five circumaxillary sutures studied were widened in multiple zones in Alt- MEC group. However, only midpalatal suture was expanded with cellular fibrous tissue filling in ME group. Significant osteoclast hyperplasia was observed in all circumaxillary sutures after alternate expan- sions and constrictions, but osteoclast count increase was only observed in midpalatal suture in ME group. These results suggested that cranial and circumaxillary sutures were actively reconstructed after Alt-MEC, while only midpalatal suture had active reaction after ME.

关键词： maxillary expansion alternate maxillary expansions and constrictions circumaxiUary su-tures

Three-dimensional Localization of Impacted Canines and Root Resorption Assessment Using Cone Beam Computed Tomography

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2014年第3期34卷 425-430页

作者： Eyad Almuhtaseb 毛靖 Derek Mahony Rawan Bader 张智星 Department of orthodontics Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Department of orthodontics London Dental School London United Kingdom Ministry of Health Kingdom of Saudi Arabia

Summary： The purpose of this study was to develop a new way to localize the impacted canines from three dimensions and to investigate the root resorption of the adjacent teeth by using cone beam computed tomography （CBCT）. Forty-six patients undergoing orthodontic treatments and having impacted canines in Tongii Hospital were examined. The images of CBCT scans were obtained from KaVo 3D exam vision. Angular and linear measurements of the cusp tip and root apex according to the three planes （mid-sagittal, occlusal and frontal） have been taken using the cephalometric tool of the InVivo Dental Anatomage Version 5.1.10. The measurements of the angular and linear coordinates of the maxillary and mandibular canines were obtained. Using this technique the operators could envision the location of the impacted canine according to the three clinical planes. Adjacent teeth root *** 28.26 % was in the upper lateral incisors while 17.39% in upper central incisors, but no lower root resorption was found in our samples. Accurate and reliable localization of the impacted canines could be obtained from the novel analysis system, which offers a better surgical and orthodontic treatment for the patients with impacted canines.

关键词： impacted canines root resorption cone beam computed tomography

RANKL deletion in periodontal ligament and bone lining cells blocks orthodontic tooth movement

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International Journal of Oral Science 2018年第1期10卷 27-35页

作者： Chia-Ying Yang Hyeran Helen Jeon Ahmed Alshabab Yu Jin Lee Chun-Hsi Chung Dana T. Graves Department of orthodontics School of Dental Medicine University of Pennsylvania Philadelphia PA USA Department of Pedodontics School of Dental Medicine University of Pennsylvania Philadelphia PA USA

The bone remodeling process in response to orthodontic forces requires the activity of osteoclasts to allow teeth to move in the direction of the force applied. Receptor activator of nuclear factor-κB ligand(RANKL) is essential for this process although its cellular source in response to orthodontic forces has not been determined. Orthodontic tooth movement is considered to be an aseptic inflammatory process that is stimulated by leukocytes including T and B lymphocytes which are presumed to stimulate bone resorption. We determined whether periodontal ligament and bone lining cells were an essential source of RANKL by tamoxifen induced deletion of RANKL in which Cre recombinase was driven by a 3.2 kb reporter element of the Col1α1 gene in experimental mice(Col1α***+.RANKLf/f) and compared results with littermate controls(Col1α***-.RANKLf/f). By examination of Col1α***+.ROSA26 reporter mice we showed tissue specificity of tamoxifen induced Cre recombinase predominantly in the periodontal ligament and bone lining cells. Surprisingly we found that most of the orthodontic tooth movement and formation of osteoclasts was blocked in the experimental mice, which also had a reduced periodontal ligament space. Thus, we demonstrate for the first time that RANKL produced by periodontal ligament and bone lining cells provide the major driving force for tooth movement and osteoclastogenesis in response to orthodontic forces.

关键词： DEPENDENT UP-REGULATION OSTEOCLAST DIFFERENTIATION ALKALINE-PHOSPHATASE MECHANICAL VIBRATION PDL FIBROBLASTS GENE-TRANSFER B-CELLS EXPRESSION TISSUE COMPRESSION

Loss of cathepsin C enhances keratinocyte proliferation and inhibits apoptosis

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Science Bulletin 2016年第14期61卷 1107-1114页

作者： Xin Li Ling-Fei Jia Yunfei Zheng Yiping Huang Man Qin Yuan Yang Department of Pediatric Dentistry Peking University School and Hospital of Stomatology Department of Oral and Maxillofacial Surgery Peking University School and Hospital of Stomatology Central Laboratory Peking University School and Hospital of Stomatology Department of orthodontics Peking University School and Hospital of Stomatology

Papillon-Lefevre Syndrome is a rare autosomal recessive disorder characterized by rapidly progressive periodontitis and confined palrnoplantar hyperkeratosis resulting from genetic mutations in cathepsin C （CTSC）. The present study investigated the effect of CTSC on keratinocyte proliferation and apoptosis. HaCaT keratinocytes were transfected with wild-type CTSC and CTSC-targeted siRNAs to investigate the effects of CTSC expression on cell keratosis. Real-time PCR and Western blot analyses showed that the levels of loricrin and keratin （KRT）-I, but not KRT9, was correlated with CTSC expression. Loricrin was increased in the CTSC-overex- pression group and downregulated in the CTSC-silenced group. A positive association between loricrin expression and cell apoptosis was detected in HaCaT keratinocytes. KRT1 was decreased in the CTSC-overexpression group and increased in the CTSC-silenced group. Prominent, punctuate KRT1 aggregates were present in CTSC-knockdown HaCaT cells. This study suggested that loss of CTSC contributes to keratinocyte hyperkeratosis via downregulation of loricrin and enhanced cell proliferation.

关键词： Papillon-Lefevre syndrome CathepsinC Loricrin Cytokeratin 1 - Cytokeratin 9

The Effect of Simvastatin on mRNA Expression of Transforming Growth Factor-β1,Bone Morphogenetic Protein-2 and Vascular Endothelial Growth Factor in Tooth Extraction Socket

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International Journal of Oral Science 2009年第2期1卷 90-98页

作者： Chang Liu Zhe Wu Hong-chen Sun Department of orthodontics School of Stomatology Jilin University Changchun China Department of Prosthodontics School of Stomatology Jilin University Changchun China Department of Oral Pathology School of Stomatology Jilin University Changchun China

Aim To determine the effect of local simvastatin application on the mRNA expression level of transforming growth factor-β1 （TGF-β1）, bone morphogenetic protein-2 （BMP-2） and vascular endothelial growth factor （VEGF） in the tooth sockets of rat. Methodology Forty-eight male Wistar rats were randomly divided into experimental and control groups （n=24）. Polylactic acid/polyglycolic acid copolymer carriers, with or without simvastatin, were implanted into extraction sockets of right mandibular incisors. The expression of TGF-β1, BMP-2 and VEGF mRNA was determined by in situ hybridization in the tooth extraction socket at five days, one week, two weeks and four weeks after implantation. Results The fusiform stroma cells in the tooth extraction socket began to express TGF-β1, BMP-2 and VEGF mRNA in both experimental and control groups from one week after tooth extraction until the end of experiment. The expression of TGF-131 and BMP-2 mRNA in the experimental group was significantly up-regulated after one, two and four weeks, and expression of VEGF mRNA was significantly increased after one and two weeks compared with that in the control group. Conclusion The findings indicate that local administration of simvastatin can influence alveolar bone remodeling by regulating the expression of a school of growth factors which are crucial to osteogenesis in the tooth extraction socket.

关键词： bone morphogenetic protein-2 (BMP-2) in situ hybridization simvastatin tooth extraction socket,transforming growth factor-β1 (TGF-β1) vascular endothelial growth factor (VEGF)

Eysenck personality and psychosocial status of adult patients with malocclusion

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Asian Pacific Journal of Tropical Medicine 2012年第2期5卷 151-156页

作者： Li Zhang Xiang Liu Gen-Jian Zheng Lan Zhou Dian-Yue Lin Xiao-Dan Wang Hong He Department of orthodontics Hubei-MOST KLOS & KLOBMSchool and Hospital of StomatologyWuhan University school of Stomatology Hainan Medical University school of Public Health Hainan Medical University

Objective:To evaluate the personality and psychosocial status of adult malocclusion patients through the Eysenck Personality Questionnaire(EPQ) and the Symptom Checklist 90(SCL-90). Methods:The EPQ and the SCL-90 were administered to 348 adult respondents with Angle’s ClassⅠ,Ⅱ,Ⅲmalocclusion and normal *** and psychosocial traits were analyzed and compared among the ***:Patients in Angle’s ClassⅠ,D andⅢgroups scored lower on the EPQ-E and higher on the EPQ-N than those in the normal occlusion group, whereas patients in the Class II group scored higher on the *** mean scores of ClassⅠ,ⅡandⅢgroups were significantly higher than those of the normal group on somatization, obsessive-compulsiveness,interpersonal sensitivity,depression,anxiety,and paranoid ideation. All SCL-90 scores were significantly positively correlated with *** and neuroticism scores of female respondents were higher than those of male *** impact of education was greatest on the EPQ-P and the EPQ-E of adult personality,whereas the impact of deformity was greatest on the EPQ-N of adult ***:Personality and psychosocial status show differences in adult patients with ClassⅠ,Ⅱ,Ⅲmalocclusion and normal occlusion and can be influenced by gender,deformity,age and education.

关键词： EPQ SCL-90 Adult patients Malocclusion

A novel FZD6 mutation revealed the cause of cleft lip and/or palate in a Chinese family

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Genes & Diseases 2020年第3期7卷 440-447页

作者： Jieni Zhang Huaxiang Zhao Wenbin Huang Fengqi Song Wenjie Zhong Mengqi Zhang Yunfan Zhang Zhibo Zhou Jiuxiang Lin Feng Chen Department of orthodontics Peking University School and Hospital of StomatologyBeijingChina Central Laboratory Peking University School and Hospital of StomatologyBeijingChina Department of orthodontics College of StomatologyXi’an Jiaotong UniversityXi’anShaanxiChina Department of Oral and Maxillofacial Surgery Peking University School and Hospital of StomatologyBeijingChina

Cleft lip and/or palate(CL/P)is a most common craniofacial birth defect which has multifactorial *** our study,we aimed to discover the underlying etiological gene variation in a Chinese family diagnosed as non-syndromic CL/P(NSCL/P).The blood sample of the proband and her parents were detected by whole exome *** Mendelian inheritance pattern,allele frequency,variation location,function analysis and literature search were applied to filtrate and screen the ***,the candidates were confirmed by Sanger *** meanwhile explored the conservative analysis and protein homology *** a result,a start-lost mutation c.1A>GAtg/Gtg in the Frizzled-6(FZD6)gene predicting ***1 was *** variation has not been reported before and was predicted to be *** alteration caused missing of two starting amino acids that are evolutionarily conserved for FZD6 ***,the specific structure of the mutant protein obviously changed according to the results of the homologous *** conclusion,the results suggest c.1A>GAtg/Gtg in the FZD6(NM_001164616)might be the genetic etiology for non-syndromic CL/P in this ***,this finding provided new etiologic information,supplementing the evidence that FZD6 is a strong potential gene for CL/P.

关键词： Cleft lip and/or palate Frizzled-6 Hereditary pedigree Variant Whole-exome sequencing