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Journal of food Science and engineering 2011年第5期1卷 374-378页

作者： I. R. Freitas G. V. Gautério D. G. Rios C. Prentice Laboratory of food Technology School of Chemistry and Food Federal University of Rio Grande

The aim of this study was to obtain a protein isolate from Argentine anchovy （Engraulis anchoita） residue produced using the pH shifting process, with acid and alkaline solubilization and isoelectric precipitation of the protein, and evaluate their functional properties. The lowest solubility was obtained at pH 5 where 8.32% and 2.96% were found for acid and alkali respectively. The highest solubility was obtained at extreme pH. The maximum water holding capacity was presented by the alkaline protein isolate at pH 11 （9.63 g H20/g protein）. The oil holding capacity was 4.20 mL/g protein, 7.26 mL/g of protein for the acid and alkaline protein isolates, respectively. The strain showed higher oil holding capacity in alkaline pH, lower solubility in pH near the protein isoelectric point and a lower capacity to retain water in extreme pH when compared with the acid isolate.

关键词： Fish anchoita protein isolates by-products.

Identification of potential sex-related genes in Siniperca chuatsi

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Journal of Oceanology and Limnology 2021年第4期39卷 1500-1512页

作者： Qiaoying ZHU Chong HAN Cheng PENG Xingni ZHOU Chongwei WANG Linqiang HAN Shuisheng LI Guifeng LI Haoran LIN Yong ZHANG State Key Laboratory of Biocontrol Guangdong Provincial Key Laboratory for Aquatic Economic Animals and Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai)School of Life SciencesSun Yat-Sen UniversityGuangzhou 510275China Laboratory for Marine Fisheries Science and food Production Processes Qingdao National Laboratory for Marine Science and TechnologyQingdao 266373China Guangdong Key Laboratory of Animal Conservation and Resource Utilization Guangdong Public Laboratory of Wild Animal Conservation and UtilizationGuangdong Institute of Applied Biological ResourcesGuangzhou 510260China Guangdong Liangshi Aquatic Seed Industry Co. Ltd.Foshan 528100China

Mandarin fish(Siniperca chuatsi)is an economically important freshwater fish cultured in *** this species,females grow faster than ***,due to the lack of available genomic and transcriptome information,the mechanisms of sex differentiation remain poorly *** this study,Illumina high-throughput sequencing technology was used to sequence four cDNA libraries;the tissues examined included hypothalamus,pituitary gland,ovary,and testis.A total of 134124 high-quality unigenes were obtained on average length of 1361 bp and N50 of 3312 bp.A search of all-unigene sequences against NR,NT,SwissProt,KOG,KEGG,GO,and InterPro databases resulted in 59688(44.50%),76329(56.91%),50432(37.60%),45741(34.10%),48760(36.35%),5241(3.91%),and 46099(34.37%)annotations,*** a comparison of ovarian and testicular libraries,15289 ovary-biased genes and 10035 testis-biased genes were identified,including a series of genes related to sex determination and differentiation,such as cyp19a1a,foxl2,sox9,dmrt1,amh,and *** addition,49495 SSRs and 85899 SNPs were detected in transcriptome *** real-time PCR results of 15 sex-related functional genes indicated that RNA-seq data was *** study will contribute to a better understanding of the molecular mechanisms of sex differentiation and development in Mandarin fish.

关键词： transcriptome Mandarin fish HPG axis female and male

Decoding the intercellular communication network during tumorigenesis

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Cancer Biology & Medicine 2022年第3期19卷 265-272页

作者： Liwei An Ruixian Yu Yi Han Zhaocai Zhou State Key Laboratory of Genetic engineering Zhongshan HospitalSchool of Life SciencesFudan UniversityShanghai 200438China Department of Medical Ultrasound Shanghai Tenth People’s HospitalTongji University School of MedicineShanghai Engineering Research Centre of Ultrasound Diagnosis and TreatmentShanghai 200072China

Under physiological conditions,decisions about cell fate and behavior are not made by the individual cell itself,but instead strictly depend on the collective interactions of the whole cell population in the ***,continuous intercellular communication influences almost all biological processes ranging from tissue/organ development,and homeostasis maintenance,to disease *** tumorigenesis,intercellular communications between cancer cells and other cells coexist within microenvironments involving stromal cells,immune cells,and even neural cells,playing crucial roles in tumor initiation,progression,and distal metastasis.

关键词： intercellular tumorigenesis homeostasis

Carbon Fiber Supported Binary Metal Sulfide Catalysts with Multi-Dimensional Structures for Electrocatalytic Nitrogen Reduction Reactions Over a Wide pH Range

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Advanced Fiber Materials 2021年第4期3卷 229-238页

作者： Tianyi Zhang Wei Zong Yue Ouyang Yue Wu Yue-E Miao Tianxi Liu State Key Laboratory for Modification of Chemical Fibers and Polymer Materials College of Materials Science and EngineeringInnovation Center for Textile Science and TechnologyDonghua University2999 North Renmin RoadShanghai 201620People’s Republic of China Research Center for Analysis and Measurement Donghua UniversityShanghai 201620People’s Republic of China Key Laboratory of Synthetic and biological Colloids Ministry of EducationSchool of Chemical and Material EngineeringJiangnan UniversityWuxi 214122People’s Republic of China

Green and environmentally friendly electrocatalytic nitrogen(N_(2))fixation to synthesize ammonia(NH3)is recognized as an effective method to replace the traditional Haber-Bosch ***,the difficulties in N_(2) adsorption and fracture of hard N≡N bond still remain major challenges in electrocatalytic N_(2) reduction reactions(NRR).From the perspectives of enhancing N_(2) adsorption and providing more catalytic sites,two-dimensional(2D)FeS_(2) nanosheets and three-dimensional(3D)metal organic framework-derived ZnS embedded within N-doped carbon polyhedras are grown on the carbon cloth(CC)template in this ***,a composite NRR catalyst with multi-dimensional structures,which is signed as FeS_(2)/ZnS-NC@CC,is obtained for using over a wide pH *** uniform distribution of hollow ZnS-NC frameworks and FeS_(2) nanosheets on the surface of CC largely increase the N_(2) enrichment efficiency and offer more active sites,while the CC skeleton acts as an independent conductive substrate and S-doping helps promote the fracture of N≡N bond during the NRR *** a result,the FeS_(2)/ZnS-NC@CC electrode achieves a high Faraday efficiency of 46.84%and NH3 yield of 58.52μg h^(−1) mg^(−1) at-0.5 V ***/AgCl in 0.1 M ***,the FeS_(2)/ZnS-NC@CC electrode displays excellent NRR catalytic activity in acidic and neutral electrolytes as well,which outperforms most previously reported electrocatalysts including noble ***,this work provides a new way for the design of multi-dimensional electrocatalysts with excellent electrocatalytic efficiency and stability for NRR applications.

关键词： Multi-dimensional structure Heteroatom doping Carbon fiber cloth Electrocatalytic nitrogen reduction

Spline regression modelling of PTO performance of tractor fuelled with different biodiesels

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International Journal of Agricultural and biological engineering 2017年第3期10卷 115-120页

作者： Ali Aybek Selcuk Arslan Mustafa Ucgul Serdar Üçok KahramanmarasSutcu Imam University Faculty of AgricultureDepartment of Biosystems EngineeringKahramanmarasTurkey Uludag University Faculty of AgricultureDepartment of Biosystems EngineeringBursaTurkey University of South Australia School of EngineeringMawson Lakes5095Australia

The objective of this study was to investigate the possibility of fitting spline regression models for power take-off(PTO)performance characteristics of an agricultural tractor tested with four different fuels,including diesel fuel(B0)and three biodiesel blends made of canola oil(B10:10%biodiesel+90%petro-diesel blend;B20:20%biodiesel+90%petro-diesel blend;B30:30%biodiesel+90%petro-diesel blend).The performance characteristics evaluated were PTO power,engine torque,engine fuel consumption,and specific fuel *** to sharp slopes in measured quantities around the nominal engine speed(2200 r/min),compared to the standard regression method,the spline regression models suited well to the experimental data with coefficient of determination R2=0.99 for PTO power and engine torque.R2 varied between 0.97 and 0.99 for fuel consumption and 0.91 and 0.95 for specific fuel *** weaker correlation found for specific fuel consumption could be attributed to profound fluctuations in measured data causing atypical pattern in the corresponding graphs around the nominal engine *** was concluded that splines were useful to accurately predict the measured PTO power and engine *** standard methods nor splines were sufficient to obtain very good regression models for specific fuel consumption.

关键词： biodiesel performance characteristics power take-off tractor spline modelling

A Review on the Coupled Method of Using the Magnetic and Acoustic Fields for biological Tissue Imaging

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Chinese Journal of Electrical engineering 2023年第1期9卷 47-60页

作者： Yuanyuan Li Guoqiang Liu Institute of Electrical engineering Chinese Academy of SciencesBeijing 100190China Institute of Electrical engineering and Advanced Electromagnetic Drive Technology Qilu ZhongkeJinan 250102China school of Electronic Electrical and Communication engineering University of Chinese Academy of SciencesBeijing 100049China

Magnetic field and acoustic field coupled imaging methods mainly include magnetoacoustic tomography,magneto-acousto-electrical tomography,and thermoacoustic tomography,all of which non-invasively achieve the electrical conductivity imaging of tissues with a resolution of up to the millimeter *** principles of these three imaging methods and the research progress in the last two decades are ***,the principles of the three magnetic and acoustic field coupled methods are individually *** progress in medical electromagnetic imaging is further elaborated,and finally the future directions and summary of the coupled imaging methods are summarized.

关键词： Biomedical imaging electromagnetic field acoustic field electrical conductivity coupled method

Recognition of weeds at asparagus fields using multi-feature fusion and backpropagation neural network

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International Journal of Agricultural and biological engineering 2021年第4期14卷 190-198页

作者： Yafei Wang Xiaodong Zhang Guoxin Ma Xiaoxue Du Naila Shaheen Hanping Mao school of Agricultural engineering Jiangsu UniversityZhenjiang 212013China Key Laboratory of Modern Agricultural Equipment and Technology Ministry of EducationJiangsu UniversityZhenjiang 212013JiangsuChina school of Electrical and Information engineering Jiangsu UniversityZhenjiang 212013JiangsuChina

In order to solve the problem of low recognition rates of weeds by a single feature,a method was proposed in this study to identify weeds in Asparagus(Asparagus officinalis L.)field using multi-feature fusion and backpropagation neural network(BPNN).A total of 382 images of weeds competing with asparagus growth were collected,including 135 of Cirsium arvense(L.)Scop.,138 of Conyza sumatrensis(Retz.)***,and 109 of Calystegia hederacea *** grayscale images were extracted from the RGB images of weeds using the 2G-R-B *** segmentation of the grayscale image of weeds was applied using Otsu *** the internal holes of the leaves were filled through the expansion and corrosion morphological operations,and other interference targets were removed to obtain the binary *** foreground image was obtained by masking the binary image and the RGB ***,the color moment algorithm was used to extract weeds color feature,the gray level co-occurrence matrix and the Local Binary Pattern(LBP)algorithm was used to extract weeds texture features,and seven Hu invariant moment features and the roundness and slenderness ratio of weeds were extracted as their shape *** to the shape,color,texture,and fusion features of the test samples,a weed identification model was *** test results showed that the recognition rate of Cirsium arvense(L.)Scop.,Calystegia hederacea *** Conyza sumatrensis(Retz.)*** were 82.72%(color feature),72.41%(shape feature),86.73%(texture feature)and 93.51%(fusion feature),***,this method can provide a reference for the study of weeds identification in the asparagus field.

关键词： weeds recognition image processing feature extraction multi-feature fusion BP neural network asparagus field

E93 promotes transcription of RHG genes to initiate apoptosis during Drosophila salivary gland metamorphosis

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Insect Science 2023年第3期30卷 588-598页

作者： Jiahui Zhang Wenhao Zhang Lin Wei Lidan Zhang Jiali Liu Shumin Huang Sheng Li Weike Yang Kang Li Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology Institute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhou510631China Guangdong Laboratory for Lingnan Modern Agriculture South China Agricultural UniversityGuangzhou510642China College of biological and food engineering Huaihua UniversityHuaihua418000China The Sericultural and Apicultural Research Institute Yunnan Academy of Agricultural SciencesMengziYunnan661100China Guangmeiyuan R&D Center Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied TechnologySouth China Normal UniversityMeizhou514779China

20-hydroxyecdysone(20E)induced transcription factor E93 is important for larval–adult transition,which functions in programmed cell death of larval obsolete tissues,and the formation of adult new ***,the apoptosis-related genes directly regulated by E93 are still *** this study,an E93 mutation fly strain was obtained by clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated protein 9-mediated long exon deletion to investigate whether and how E93 induces apoptosis during larval tissues *** transcriptional profile of E93 was consistent with 3 RHG(rpr,hid,and grim)genes and the effector caspase gene drice,and all their expressions peaked at the initiation of apoptosis during the degradation of salivary *** transcription expression of 3 RHG genes decreased and apoptosis was blocked in E93 mutation salivary gland during *** contrast,E93 overexpression promoted the transcription of 3 RHG genes,and induced advanced apoptosis in the salivary ***,E93 not only enhance the promoter activities of the 3 RHG genes in Drosophila Kc cells in vitro,but also in the salivary gland in *** results demonstrated that 20E induced E93 promotes the transcription of RHG genes to trigger apoptosis during obsolete tissues degradation at metamorphosis in Drosophila.

关键词： apoptosis Drosophila E93 RHG genes salivary gland transcription

Spectrophotometric Determination of Arginine in Grape Juice Using 8-Hydroquinoline

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Agricultural Sciences in China 2008年第10期7卷 1210-1215页

作者： WANG Hua LIANG Xin-hong ZHAO Rui-xiang FENG Li-dan LI Hua College of Enology Northwest A ＆ F University Yangling 712100 P.R.China school of food Science Henan Institute of Science and Technology Xinxiang 453003 P.R.China Shaanxi engineering Research Center for Viti-Viniculture Yangling 712100 P.R.China

Arginine in grape juice can be metabolized by wine yeasts and malolactic bacteria to precursors of ethyl carbamate, known as carcinogen. The aim of this study was to develop a simple, fast, and accurate method for determining arginine in grape juice with Sakaguchi reaction by separating arginine with strong cation-exchange resins. Parameters were optimized including the concentrations of 8-hydroquinoline and sodium hydrobromite. The color stability lasted for 4 min, which is sufficient to finish the measurement. The method is simple, reproducible and accurate, and can be applied for quick measurement of arginine in grape juice to take necessary measures for controlling the level of ethyl carbamate.

关键词： grape juice L-arginine ethyl carbamate Sakaguchi reaction