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Co-expression and Immunity of Legionella pneumophila mip Gene and Immunoadjuvant ctxB Gene

Acta Biochimica et Biophysica Sinica 2005年第3期37卷 199-204页

作者： Tao WANG Jian-Ping CHEN Hong LI Ke-Qian ZHI Lei ZHANG Chun-Lei YANG Da-Chang TAO school of Precliaical and forensic Medicine of West China,Sichuan University,Chengdu 610041,China school of Stomatolngy,Xi'an Jiaotong University,Xi'an 710004,China school of Life science,Sichuan University,Chengdu 610041,China school of Preclinical and forensic Medicine of West China Sichuan UniversityChengdu 610041 China school of Stomatology Xi'an Jiaotong UniversityXi'an 710004 China school of Life science Sichuan UniversityChengdu 610041 China

The mip gene of Legionella pneumophila and the ctxB gene of Vibrio cholerae were amplifiedby PCR *** amplified cDNA was ligated to the pcDNA3.1(+)*** recombinant plasmidspcDNA3,1-mip and pcDNA3.1-ctxB were identified by restriction analysis and PCR,and further confirmedby sequencing ***3T3 cells were transfected with pcDNA3.1-mip and pcDNA3.1-ctxB accordingto the Lipofection *** and stable products of the co-expression of the mip gene and ctxB genewere detected by immunofluorescence and Western *** results showed that NIH3T3 cells weresuccessfully transfected,and that the transiently and stably co-expressed products can be detected in thetransfected *** detect the humoral and cellular immune response in immunized mice induced by the co-immunization of the mip and ctxB genes,female BALB/c mice were immunized intramuscularly with pcDNA3.1-mip and *** results showed that the specific antibody titer and the cytotoxic T-lymphocyteresponse for pcDNA3,1-mip immunization and co-immunization were increased compared with that ofpcDNA3.1(+) ***,the specific antibody titer and cytotoxic T-lymphocyte responsefor co-immunization were increased compared with that of pcDNA3.1-mip *** analysisusing one-way analysis of variance(ANOVA)showed that there was a significant difference between thegroups(P<0.01).The results indicated that the ctxB gene enhanced the humoral and cellular immune responseto the mip gene *** findings provide experimental evidence to support the development ofthe *** DNA vaccine.

关键词： Legionella pneumophila co-expression humoral immunity cellular immunity

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Characteristics of Mechanical Asphyxia:A Retrospective Epidemiological Study in Two Developed Cities in China

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Journal of forensic science and Medicine 2023年第1期9卷 57-63,I0001,I0002页

作者： Erwen Huang Guoli Lv Yumei Wang Qiangwei Wang Jing Zhang Lie Liang Ben Liu Wen Yang Chuanyi Tang Chao Liu Jianding Cheng Guangdong Province Translational forensic Medicine Engineering Technology Research Center Faculty of Forensic MedicineZhongshan School of MedicineSun Yat-Sen UniversityGuangzhouChina Guangzhou forensic science Institute GuangzhouChina The Institute of criminal science and Technology of Dongguan Public Security Bureau DongguanChina Guangzhou Public Security Bureau GuangzhouChina

Background:Mechanical asphyxia(MA)is *** epidemiological characteristics are shared and the others different among different MA *** and Objectives:To reveal the characteristics of different types of MA and provide reference data for forensic identification of *** and Methods:We analyzed 913 cases to reveal the epidemiological characteristics of MA in Dongguan and Guangzhou,***:Seven types of MA were identified and strangulation accounted for up to 89.2%.Those aged 20-29 were the most susceptible to all types,except for traumatic and positional *** accounted for 96.6%of hanging cases,and accident was the main manner of death in cases of choking,traumatic,and positional *** was the most common in cases of ligature strangulation(LS),manual strangulation(MS),smothering,and asphyxia concomitant with lethal mechanical *** homicide rate in LS was positively correlated with the rate of female victims(P<0.001,OR=21.14).In MS,the ratio of female:male was 101:15 in *** to native residents,migrant workers were more likely to suffer from LS and *** was used in 50.8%of hanging cases,while LS tools were evenly *** marks were mostly found above the thyroid cartilage in both hanging and LS *** U shaped marks were shown in 51.01%of hanging cases but absent in LS *** LS,ligature marks completely encircling the neck,discontinuous marks,as well as≥two marks,were more(P<0.001)than in *** LS and MS cases,abrasion and subcutaneous hemorrhages on the neck were more frequent than in hanging cases(P=0.004 and P<0.001),but the difference in frequency of blister marks was opposite(P<0.001).Conclusion:In summary,this study revealed the epidemiological characteristics of the MA cases in Guangzhou and Dongguan *** characteristics of death scene and autopsy findings are important in differentiation of different types of MA,especially between suicide and homicide.

关键词： Hanging ligature strangulation manual strangulation mechanical asphyxia smothering

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Deoxyribozymes inhibit the expression of periodl gene in vitro

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science China(Life sciences) 2005年第3期48卷 195-201页

作者： ZHOU Wei WANG Yueqi LIU Yanyou PENG Wenzhen XIAO Jing ZHU Bin WANG Zhengrong Biomedical Engineering Department School of West China Basic and Forensic ScienceSichuan UniversityChengdu610041China Molecular Biology and Biology Chemistry Department School of West China Basic and Forensic ScienceSichuan UniversityChengdu610041China

To investigate the effect of two deoxyribozymes targeting period1(per1)mRNA in vitro for exploring a novel gene therapy approach about circadian rhythm diseases,the specific deoxyribozymes targeting per1 were designed and synthesized chemically following MFold analysis according to its mRNA secondary ***1 RNA fragments were prepared by in vitro transcription of pcDNA3.1(+)-per1_(164:256).The cleavage reactions containing deoxyribozymes and per1 RNA fragments were performed under certain *** the transfection tech-nique mediated by LipofectAMINE^(TM),pcDNA3-per1 and DRz164 or DRz256 were introduced into NIH3T3 *** effects of deoxyribozymes on per1 were studied by reverse tran-script-polymerase chain reaction(RT-PCR)and flow cytometry(FCM).When deoxyribozymes and RNA transcripts were incubated under the adopted conditions at 37℃for 2 h,about 63%of per1_(164:256)RNA transcripts were cleaved by DRz164 and about 50.5%by *** co-transfecting pcDNA3-per1 with DRz164 or DRz256,the expression of per1 mRNA was de-creased,as indicated by RT-PCR semi-quantity *** analysis showed that Per1 protein was *** DRz164 and DRz256 targeting per1 have the specific cleavage activity to-ward per1 mRNA in vitro and can highly block the expression of per1 gene in cellular milieu.

关键词： period1 gene deoxyribozymes cleavage in vitro gene transfection.

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Activated carbon fibers with manganese dioxide coating for flexible fiber supercapacitors with high capacitive performance

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Journal of Energy Chemistry 2019年第4期28卷 95-100页

作者： Huifang Li Jiachen Liang Huan Li Xiaoyu Zheng Ying Tao Zheng-Hong Huang Quan-Hong Yang Nanoyang Group School of Chemical Engineering and Technology Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) Tianjin University Institute of forensic science Ministry of Public Security Laboratory of Advanced Materials School of Materials Science and Engineering Tsinghua University

Fiber supercapacitor(FSC)is a promising power source for wearable/stretchable electronics and high capacitive performance of FSCs is highly desirable for practice flexible ***,we report a composite of manganese dioxide(Mn O_2)and activated carbon fibers(ACFs)with high MnO_2mass loading and microporous structure(abbreviated as Mn O_2@ACF),which is used as a fiber electrode to produce a FSC with a high capacitive performance and a good *** MnO_2@ACF composite electrode in FSCs delivers an ultrahigh specific capacitance of 410 mF/cm^2at 0.1 mA/cm^2,corresponding to a high energy density of 36μWh/cm^2and high power density of 726μW/cm^*** high capacitive performance and simple fabrication method indicates that the Mn O_2@ACF composite is a very promising electrode material for flexible fiber supercapacitors.

关键词： Fiber supercapacitors Activated carbon fibers Mass loading Flexibility

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Objective evaluation of similarity scores derived by Evofinder^(®) system for marks on bullets fired from Chinese Norinco QSZ-92 pistols

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forensic sciences Research 2022年第1期7卷 40-46页

作者： Feng Dong Yabin Zhao Yaping Luo Weifang Zhang Yuesong Li school of forensic science People’s Public Security University of ChinaBeijingChina Shanghai Key Laboratory of Crime Scene Evidence Shanghai Research Institute of Criminal Science and TechnologyShanghaiChina Graduate school People’s Public Security University of ChinaBeijingChina

In recent years,many studies have been conducted in the field of firearm identification with the objective of providing an objective method of evaluating the comparison of cartridge ***,less attention has been paid to the objective evaluation of bullet *** this study,1000 registered Chinese Norinco QSZ-92 pistols were used,and a database of 2996 bullets was *** the receiver operating characteristic(ROC)curve and the score-based likelihood ratio method were used to objectively evaluate the similarity scores derived by the EvofinderVR *** results indicate that this system has excellent ability to discriminate between the selected *** paper proposes an objective evaluation method,which serves as a response to the ongoing debates regarding the foundation of the discipline.

关键词： forensic sciences firearm identification EvofinderVR likelihood ratio automatic correlation

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Determining the Electrical Conductivity of Rat Cadaveric Liver,Spleen,and Kidney to Estimate Early Postmortem Interval

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Journal of forensic science and Medicine 2017年第3期3卷 111-114页

作者： Zhe Zheng Xiandun Zhai Zhiyuan Xia Yaonan Mo Department of forensic Medicine Forensic Medicine SchoolHenan University of Science and TechnologyLuoyangHenanChina

Previous research has found that electrical conductivity(EC),an important index to predict meat freshness and shelflife,is very promising for estimating the late postmortem interval(PMI).However,whether it has potential use in the early PMI has not been fully studied *** test this possibility in the present study,EC of three internal organs of rat liver,spleen,and kidney were determined within 24 postmortem hows,and then,EC changes at different PMIs were carefully *** overall results showed that EC of liver and spleen increased significantly with PMI while EC of kidney had minor changes during the same ***,significant linear positive correlations between EC of liver and spleen and PMI were found and the coefficients of their regression functions were R^(2)=0.98 and R^(2)=0.95,*** can be concluded that determination of EC in certain internal organs such as liver and spleen may be a potential tool in the early PMI ***,more researches on its influencing factors are needed to facilitate its final use in practice.

关键词： Early postmortem interval electrical conductivity forensic science kidney liver spleen

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Preliminary study on the mechanisms of ankle injuries under falling and impact conditions based on the THUMS model

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forensic sciences Research 2022年第3期7卷 518-527页

作者： Zhengdong Li Jianhua Zhang Jinming Wang Ping Huang Donghua Zou Yijiu Chen Department of forensic Medicine School of Basic Medical SciencesFudan UniversityShanghaiChina Shanghai Key laboratory of forensic Medicine Academy of Forensic ScienceMinistry of JusticeShanghaiChina

Ankle injuries are common in forensic practice,which are mainly caused by falling and traffic *** the mechanisms and manners of ankle injuries is a critical and challenging problem for forensic *** identification of the injury mechanism is still experience-based and strongly *** also lacks systematic research in current *** our study,based on the widely used Total Human Model of Safety 4.0(THUMS 4.0),we utilized the finite element(FE)method to simulate ankle injuries caused by falls from different heights(5 m,10 m and 20 m)with different landing postures(natural posture,inversion,eversion,plantar-flexion and dorsi-flexion)and injuries caused by impacts from different directions(anterior-posterior,lateral-medial and posterior-anterior)with different speeds(10 m/s,15m/s and 20m/s)at different sites(ankle and lower,middle and upper sections of leg).We compared the injury morphology and analyzed the mechanisms of ankle *** results showed that falling causes a specific compression fracture of the distal tibia,while fractures of the tibia and fibula diaphysis and ligament injuries caused by falling from a lower height or inversion,planter flexion or dorsiflexion at a large angle are not distinguishable from the similar injury patterns caused by impact on the middle and upper segments of the *** obvious compression fracture of the tibia distal was caused by the impacts,whereas ligament injuries and avulsion fractures of the medial or lateral condyle and fractures of the diaphysis of the tibia and fibula were *** studies will be helpful in reconstructing the ankle injury processes and analyzing the mechanisms in forensic practice,providing a deeper understanding of ankle injury mechanisms for forensic experts.

关键词： forensic sciences ankle injuries fall and impact injury finite element analysis injury biomechanisms

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A Novel Fine-Grained Method for Vehicle Type Recognition Based on the Locally Enhanced PCANet Neural Network

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Journal of Computer science & Technology 2018年第2期33卷 335-350页

作者： Qian Wang You-Dong Ding school of Computer Enqineering and science Shan qhai University Shanqhai 200072 China Information Center Criminal Investigation Department of Shanghai Public Security Bureau Shanghai 200083 China Shanghai Engineering Research Center of Motion Picture Special Effects Shanghai 200072 China Shanghai Film Academy Shanghai University Shanghai 200072 China

In this paper, we propose a locally enhanced PCANet neural network for fine-grained classification of vehicles. The proposed method adopts the PCANet unsupervised network with a smaller number of layers and simple parameters compared with the majority of state-of-the-art machine learning methods. It simplifies calculation steps and manual labeling, and enables vehicle types to be recognized without time-consuming training. Experimental results show that compared with the traditional pattern recognition methods and the multi-layer CNN methods, the proposed method achieves optimal balance in terms of varying scales of sample libraries, angle deviations, and training speed. It also indicates that introducing appropriate local features that have different scales from the general feature is very instrumental in improving recognition rate. The 7-angle in 180° （12-angle in 360°） classification modeling scheme is proven to be an effective approach, which can solve the problem of suffering decrease in recognition rate due to angle deviations, and add the recognition accuracy in practice.

关键词： fine-grained classification PCANet local enhancement vehicle type recognition

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Genetic characterization of four dog breeds with Illumina CanineHD BeadChip

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forensic sciences Research 2019年第4期4卷 354-357页

作者： Zihao Yang Jingyi Zhang Jiashuo Zhang Ruiyang Tao Wei Ren Jie Zhang Jilin Dong Chengtao Li Suhua Zhang Department of forensic Medicine School of Basic Medical ScienceWenzhou Medical UniversityWenzhouChina Shanghai Key Laboratory of forensic Medicine Shanghai Forensic Service PlatformAcademy of Forensic SciencesMinistry of JusticeP.R.ChinaShanghaiChina Department of forensic science Medical School of Soochow UniversitySuzhouChina Institute of forensic Medicine West China School of Basic Medical Sciences&Forensic MedicineSichuan UniversityChengduChina criminal police detachment of Qingdao Public Security Bureau QingdaoChina Department of forensic Medicine School of Basic Medical ScienceWenzhou Medical University Department of forensic science Medical School of Soochow UniversitySuzhouChina Institute of Forensic MedicineWest China School of Basic Medical Sciences&Forensic MedicineSichuan UniversityChengduChina

Dear Editor,Due to human’s cohabitation with domesticated animals,molecular analysis of animal DNA is increasingly being admitted as evidence in forensic *** 2011,recommendations from the International Society of forensic Genetics(ISFG)for non-human DNA analysis in forensic casework were published based on the successful model for human DNA[1].Among domesticated animals,canine DNA is perhaps the most often encountered and investigated in the forensic community[2–5].The US,Brazil and China are the top three countries in regards to ownership of *** DNA in the form of hair,saliva,blood,urine and feces is abundant in the domestic environment and consequently is often present on evidence collected during forensic investigations.A strong need for identity identification,parentage verification and breed recognition has become apparent within the forensic community.

关键词： habitat admitted characterization

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Developmental validation of the novel six-dye Goldeneye^(TM)DNA ID System 35InDel kit for forensic application

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forensic sciences Research 2022年第4期7卷 673-684页

作者： Qi Yang Huan Yu Yiling Qu Xiaochun Zhang Ruocheng Xia Ziwei Wang Rui Tan Lei Xiong Shihan Xi Jun Wu Yuzhen Gao Suhua Zhang Chengtao Li Shanghai Key Laboratory of forensic Medicine Shanghai Forensic Service PlatformAcademy of Forensic SciencesMinistry of JusticeShanghaiChina Department of forensic science Medical School of Soochow UniversitySuzhouChina Department of forensic Medicine School of Basic Medical ScienceWenzhou Medical UniversityWenzhouChina school of Basic Medicine Inner Mongolia Medical UniversityHohhotChina Clinical Medical school Inner Mongolia University for the NationalitiesTongliaoChina PEOPLESPOTINC BeijingChina

Insertion/deletion polymorphisms(InDels)have been treated as a prospective and helpful genetic marker in the fields of forensic human identification,anthropology and population genetics for the past few *** this study,we developed a six-dye multiplex typing system consisting of 34 autosomal InDels and Amelogenin for forensic *** contained InDels were specifically selected for Chinese population with the MAF≥0.25 in East Asia,which do not overlap with the markers of Investigator^(■)DIPplex *** typing system was named as GoldeneyeTM DNA ID System 35InDel Kit,and a series of developmental validation studies including repeatability/reproducibility,concordance,accuracy,sensitivity,stability,species specificity and population genetics were conducted on this *** confirmed that the 35InDel kit is precise,sensitive,species specific and robust for forensic ***,the 35InDel kit is capable of typing DNA extracted from forensic routine case-type samples as well as degraded samples and mixture *** markers are proved to be highly polymorphic with an average observed heterozygosity(He)of *** combined power of discrimination(CPD)is 0.999999999999978 and the combined power of exclusion in duos(CPE_(D))and trios(CPE_(T))are 0.978837 and 0.999573,respectively,which are higher than those of the Investigator^(■)DIPplex ***,the GoldeneyeTM DNA ID System 35InDel kit is suitable for forensic human identification and could serve as a supplementary typing system for paternity testing.

关键词： forensic sciences forensic genetics insertion and deletion polymorphism(InDel) 35InDel kit developmental validation capillary electrophoresis(CE)

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