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Generation of Eco-Friendly and Disease-Resistant Channel Catfish(Ictalurus punctatus)Harboring the Alligator Cathelicidin Gene via CRISPR/Cas9 Engineering

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Engineering 2024年第8期39卷 273-286页

作者： Jinhai Wang Baofeng Su De Xing Timothy J.Bruce Shangjia Li Logan Bern Mei Shang Andrew Johnson Rhoda Mae C.Simora Michael Coogan Darshika U.Hettiarachchi Wenwen Wang Tasnuba Hasin Jacob Al-Armanazi Cuiyu Lu Rex A.Dunham school of Fisheries Aquacultureand Aquatic SciencesAuburn UniversityAuburnAL 36849USA

As a precise and versatile tool for genome manipulation,the clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)platform holds promise for modifying fish traits of *** the aim of reducing transgene introgression and controlling reproduction,upscaled disease resistance and reproductive intervention in catfish species have been studied to lower the potential environmental risks of the introgression of escapees as transgenic *** advantage of the CRISPR/Cas9-mediated system,we succeeded in integrating the cathelicidin gene(As-Cath)from an alligator(Alligator sinensis)into the target luteinizing hormone(lh)locus of channel catfish(Ictalurus punctatus)using two delivery systems assisted by double-stranded DNA(dsDNA)and single-stranded oligodeoxynucleotides(ssODNs),*** this study,high knock in(KI)efficiency(22.38%,64/286)but low ontarget events was achieved using the ssODN strategy,whereas adopting a dsDNA as the donor template led to an efficient on-target KI(10.80%,23/213).The on-target KI of As-Cath was instrumental in establishing the lh knockout(LH^(–)_As-Cath^(+))catfish line,which displayed heightened disease resistance and reduced fecundity compared with the wild-type(WT)sibling ***,administration of human chorionic gonadotropin(HCG)and luteinizing hormone-releasing hormone analogue(LHRHa)can restore the reproduction of the transgenic fish ***,we replaced the lh gene with an alligator cathelicidin transgene and then administered hormone therapy to move towards complete reproductive control of diseaseresistant transgenic catfish in an environmentally responsible *** strategy not only effectively improves consumer-valued traits but also guards against unwanted introgression,providing a breakthrough in aquaculture genetics to confine fish reproduction and prevent the establishment of transgenic or domestic genotypes in the natural environment.

关键词： Genome editing ssODN dsDNA Antimicrobial peptide Reproductive confinement Aquaculture

Triangle-free Graphs with Three Positive Eigenvalues

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Algebra Colloquium 2024年第3期31卷 525-540页

作者： Fang Duan school of Mathematical sciences Xinjiang Normal UniversityUrumqi 830017China

A graph G is called triangle-free if G does not contain any triangle as its induced *** G_(n)be the set of triangle-free graphs of order n each of which has three positive *** this paper,we find 20 specific graphs in G_(n),each of which has nullity no more than 2,and we show that in terms of three graph transformations all the other graphs of G_(n)can be constructed from these 20 specific ***,we completely characterize the triangle-free graphs with exactly three positive eigenvalues.

关键词： positive(negative)inertia index nullity triangle-free graph

Maximal operators of pseudo-differential operators with rough symbols

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Applied Mathematics(A Journal of Chinese Universities) 2024年第1期39卷 174-180页

作者： Ramla Benhamoud ZHU Xiang-rong school of Mathematical sciences Zhejiang Normal UniversityJinhua 321004China

Consider a pseudo-differential operator T_(a)f(x)=∫_(R^(n))e^(ix,ζ)a(x,ζ)f(ζ)dζwhere the symbol a is in the rough Hormander class L^(∞)S_(ρ)^(m)with m∈R andρ∈[0,1].In this note,when 1≤p≤2,if n(ρ-1)/p and a∈L^(∞)S_(ρ)^(m),then for any f∈S(R^(n))and x∈R^(n),we prove that M(T_(a)f)(x)≤C(M(|f|^(p))(x))^(1/p) where M is the Hardy-Littlewood maximal *** theorem improves the known results and the bound on m is sharp,in the sense that n(ρ-1)/p can not be replaced by a larger constant.

关键词： pseudo-differential operator rough Hormander class H-L maximal operator

Effects of asymmetric coupling and boundary on the dynamic behaviors of a random nearest neighbor coupled system

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Chinese Physics B 2024年第6期33卷 309-322页

作者：徐玲姜磊 school of Mathematical sciences Sichuan Normal UniversityChengdu 610066China school of Mathematics and Statistics Linyi UniversityLinyi 276000China

This study investigates the dynamical behaviors of nearest neighbor asymmetric coupled systems in a confined ***, the study derivative analytical stability and synchronization conditions for the asymmetrically coupled system in an unconfined space, which are then validated through numerical simulations. Simulation results show that asymmetric coupling has a significant impact on synchronization conditions. Moreover, it is observed that irrespective of whether the system is confined, an increase in coupling asymmetry leads to a hastened synchronization pace. Additionally, the study examines the effects of boundaries on the system's collective behaviors via numerical experiments. The presence of boundaries ensures the system's stability and synchronization, and reducing these boundaries can expedite the synchronization process and amplify its effects. Finally, the study reveals that the system's output amplitude exhibits stochastic resonance as the confined boundary size increases.

关键词： asymmetric coupled confined space synchronization stochastic resonance stability

Design of zero-determinant strategies and its application to networked repeated games

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Science China(Information sciences) 2024年第10期67卷 227-243页

作者： Daizhan CHENG Changxi LI Key Laboratory of Systems and Control Academy of Mathematics and Systems SciencesChinese Academy of Sciences school of Mathematics Shandong University

Using the semi-tensor product（STP） of matrices, the profile evolutionary equation（PEE） for repeated finite games is obtained. By PEE, the zero-determinant（ZD） strategies are developed for general finite games. A formula is then obtained to design ZD strategies for general finite games with multiple players and asymmetric strategies. A necessary and sufficient condition is obtained to ensure the availability of the designed ZD strategies. It follows that player i can unilaterally design ki-1（one less than the number of its strategies） dominating linear relations about the expected payoffs of all players. Finally, the fictitious opponent player is proposed for networked repeated games（NRGs）. A technique is proposed to simplify the model by reducing the number of frontier strategies.

关键词： finite repeated game profile evolutionary equation ZD strategy networked repeated games semi-tensor product of matrices

Stability of Gas Diffusion Flame in Laterally Confined Space

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Journal of Thermal Science 2024年第6期33卷 2437-2449页

作者： WANG Xiuzhen ZHU Feng WANG Shuangfeng Key Laboratory of Microgravity Institute of MechanicsChinese Academy of Sciences school of Engineering Science University of Chinese Academy of Sciences

Lateral-confined coaxial jet diffusion flame is common in micro thrusters,and the specific impulse is mainly obtained through thermodynamic calculations with an assumption of fuel combustion with an equivalence ratio,regardless of the stability of the combustion ***,the flame behavior plays an important impact on the performance of a micro thruster through the varied combustion *** stability of confined coaxial jet diffusion flames with air coflow was studied by experiments and numerical ***,hydrogen,and propane were used as *** attachment,liftoff,blowout(extinction limits of lifted flame),and blowoff(extinction limits of attached flame) behaviors with the effect of confinement ratios and fuel properties were focused *** the range of the jet flow velocity in this research,the hydrogen flame is always attached to the jet exit,the flame tip goes from closed to open as the jet velocity increases,while the flame transitions from attachment to liftoff in the case of CH4 and ***,in a narrow confined space,the attached flame for both CH4 and C3H8 undergoes liftoff followed by ***,in a space with a high confinement ratio,the CH4 flame transitions directly from attachment to *** critical modified Craya-Curtet number,which is used to predict the onset of the recirculation,is determined through simulation and experiment,and the number is about *** work provides valuable data on flame stability inside a confined space and gives insights into the design of a thruster.

关键词： confined space Craya-Curtet number flame stability jet diffusion flame

SAT-Based Automatic Searching for Differential and Linear Trails: Applying to CRAX

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Chinese Journal of Electronics 2024年第1期33卷 72-79页

作者： Yiyi HAN Caibing WANG Zhongfeng NIU Lei HU State Key Laboratory of Information Security Institute of Information Engineering Chinese Academy of Sciences school of Cyber Security University of Chinese Academy of Sciences

Boolean satisfiability problem(SAT) is now widely applied in differential cryptanalysis and linear cryptanalysis for various cipher *** generated many excellent results for some ciphers,for example,*** this research,we study the differential and linear propagations through the operations of addition,rotation and XOR(ARX),and construct the SAT *** apply the models to CRAX to search differential trails and linear trails *** this sense,our contnbution can be broadly divided into two *** give the bounds for differential and linear cryptanalysis of Alzette both up to 12 steps,by which we present a 3-round differential attack and a 3-round linear attack for *** construct a 4-round key-recovery attack for CRAX with time complexity289times of 4-round encryption and data complexity 225.

关键词： Ciphers Encryption Complexity theory Time complexity

Compensated acceleration feedback based active disturbance rejection control for launch vehicles

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Chinese Journal of Aeronautics 2024年第4期37卷 464-478页

作者： Xiaoyan ZHANG Wenchao XUE Zibo LIU Ran ZHANG Huifeng LI Key Laboratory of Systems and Control Institute of Systems ScienceAcademy of Mathematics and Systems ScienceChinese Academy of SciencesBeijing 100190China school of Mathematical sciences University of Chinese Academy of SciencesBeijing 100049China school of Astronautics Beihang UniversityBeijing 100191China

In this paper, the attitude tracking and load relief control problems against wind disturbances and uncertain aerodynamics as well as the engine thrust of launch vehicles are ***, a framework of Compensated Acceleration Feedback based Active Disturbance Rejection Control(CAF-ADRC) is established to achieve both desired attitude tracking and load relief performances. In particular, the total disturbance that includes the effects caused by both aerocoefficient perturbations and disturbances is estimated by constructing an Extended State Observer(ESO) to achieve attitude tracking. Furthermore, combined with the normal acceleration due to the engine thrust, the accelerometer measurement is also compensated to enhance the load relief ***, the quantitative analysis of ESO and the entire closed-loop system are studied. It can be concluded that the desired attitude tracking and load relief performances can be achieved simultaneously under the proposed approach. Besides, tuning laws of the proposed approach are systematically given, which are divided into ESO, Proportional Derivative(PD) and Compensated Acceleration Feedback(CAF) modules. Moreover, the performances under CAF-ADRC approach can be better than those under CAF based PD(CAF-PD) approach by tuning load relief ***, the approach presented is applied to a typical control problem of launch vehicles with wind disturbances and parameter uncertainties.

关键词： Launch vehicles Uncertainty analysis Active disturbance rejection control(ADRC) Load relief control Extended state observer(ESO)

Controlling the dynamic behavior of decentralized cluster through centralized approaches

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Chinese Physics B 2024年第6期33卷 46-54页

作者：袁大明王培龙王鹏马星宇汪楚云王璟陈怀城王高叶方富 school of Ophthalmology and Optometry Eye HospitalSchool of Biomedical EngineeringWenzhou Medical UniversityWenzhou 325035China Postgraduate Training Base Alliance Wenzhou Medical UniversityWenzhou 325035China Wenzhou Institute University of Chinese Academy of SciencesWenzhou 325011China school of Physical sciences University of Chinese Academy of SciencesBeijing 100049China

How to control the dynamic behavior of large-scale artificial active matter is a critical concern in experimental research on soft matter, particularly regarding the emergence of collective behaviors and the formation of group patterns. Centralized systems excel in precise control over individual behavior within a group, ensuring high accuracy and controllability in task execution. Nevertheless, their sensitivity to group size may limit their adaptability to diverse tasks. In contrast, decentralized systems empower individuals with autonomous decision-making, enhancing adaptability and system robustness. Yet, this flexibility comes at the cost of reduced accuracy and efficiency in task execution. In this work, we present a unique method for regulating the centralized dynamic behavior of self-organizing clusters based on environmental interactions. Within this environment-coupled robot system, each robot possesses similar dynamic characteristics, and their internal programs are entirely identical. However, their behaviors can be guided by the centralized control of the environment, facilitating the accomplishment of diverse cluster tasks. This approach aims to balance the accuracy and flexibility of centralized control with the robustness and task adaptability of decentralized control. The proactive regulation of dynamic behavioral characteristics in active matter groups, demonstrated in this work through environmental interactions, holds the potential to introduce a novel technological approach and provide experimental references for studying the dynamic behavior control of large-scale artificial active matter systems.

关键词： self-organizing system centralized control dynamics regulation