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Effect of lamellarization on the microstructure and mechanical properties of marine 10Ni5CrMoV steel

International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 402-413页

作者： Tao Zou Yanwu Dong Zhouhua Jiang Shuyang Du Yushuo Li school of Metallurgy Northeastern UniversityShenyang 110819China key laboratory of Rolling and Automation Northeastern UniversityShenyang 110819China key laboratory for Ecological Metallurgy of Multimetallic Ores(ministry of education) Northeastern UniversityShenyang 110819China

Multistage heat treatment involving quenching(Q),lamellarizing(L),and tempering(T)is applied to marine 10Ni5CrMoV *** microstructure and mechanical properties were studied by multiscale characterizations,and the kinetics of reverse austenite transformation,strain hardening behavior,and toughening mechanism were further *** lamellarized specimens possess low yield strength but high toughness,especially cryogenic *** leads to the development of film-like reversed austenite at the martensite block and lath boundaries,refining the martensite structure and lowering the equivalent grain *** analysis of austenite reversion based on the JMAK model shows that the isothermal transformation is dominated by the growth of reversed austenite,and the maximum transformation of reversed austenite is reached at the peak temperature(750℃).The strain hardening behavior based on the modified Crussard-Jaoul analysis indicates that the reversed austenite obtained from lamellarization reduces the proportion of martensite,significantly hindering crack propagation via martensitic transformation during the *** a consequence,the QLT specimens exhibit high machinability and low yield *** with the QT specimen,the ductile-brittle transition temperature of the QLT specimens decreases from-116 to-130℃due to the low equivalent grain size and reversed austenite,which increases the cleavage force required for crack propagation and absorbs the energy of external load,*** work provides an idea to improve the cryogenic toughness of marine 10Ni5CrMoV steel and lays a theoretical foundation for its industrial application and comprehensive performance improvement.

关键词： 10Ni5CrMoV steel lamellarizing reversed austenite cryogenic toughness ductile-brittle transition temperature

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MoS_(2)Lubricate‑Toughened MXene/ANF Composites for Multifunctional Electromagnetic Interference Shielding

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Nano-Micro Letters 2025年第2期17卷 358-371页

作者： Jiaen Wang Wei Ming Longfu Chen Tianliang Song Moxi Yele Hao Zhang Long Yang Gegen Sarula Benliang Liang Luting Yan Guangsheng Wang school of Physical Science and Engineering Beijing Jiaotong UniversityBeijing 100044People’s Republic of China key laboratory of Bio‑Inspired Smart Interfacial Science and Technology of ministry of education School of ChemistryBeihang UniversityBeijing 100191People’s Republic of China

The design and fabrication of high toughness electromagnetic interference(EMI)shielding composite films with diminished reflection are an imperative task to solve electromagnetic pollution *** MXene/ANF(aramid nanofibers)–MoS_(2)composite films with nacre-like layered structure here are fabricated after the introduction of MoS_(2)into binary MXene/ANF composite *** introduction of MoS_(2)fulfills an impressive“kill three birds with one stone”improvement effect:lubrication toughening mechanical performance,reduction in secondary reflection pollution of electromagnetic wave,and improvement in the performance of photothermal *** the introduction of MoS_(2)into binary MXene/ANF(mass ratio of 50:50),the strain to failure and tensile strength increase from 22.1±1.7%and 105.7±6.4 MPa and to 25.8±0.7%and 167.3±9.1 MPa,*** toughness elevates from 13.0±4.1 to 26.3±0.8 MJ m^(−3)(~102.3%)*** the reflection shielding effectiveness(SE_(R))of MXene/ANF(mass ratio of 50:50)decreases~10.8%.EMI shielding effectiveness(EMI SE)elevates to 41.0 dB(8.2–12.4 GHz);After the introduction of MoS_(2)into binary MXene/ANF(mass ratio of 60:40),the strain to failure increases from 18.3±1.9%to 28.1±0.7%(~53.5%),the SE_(R)decreases~22.2%,and the corresponding EMI SE is 43.9 *** MoS_(2)also leads to a more efficient photothermal conversion performance(~45 to~55℃).Additionally,MXene/ANF–MoS_(2)composite films exhibit excellent electric heating performance,quick temperature elevation(15 s),excellent cycle stability(2,2.5,and 3 V),and long-term stability(2520 s).Combining with excellent mechanical performance with high MXene content,electric heating performance,and photothermal conversion performance,EMI shielding ternary MXene/ANF–MoS_(2)composite films could be applied in many industrial *** work broadens how to achieve a balance between mechanical properties and versatility of composites in the case of high-function fillers.

关键词： MXene-MoS_(2) Lubrication toughening EMI shielding Photothermal conversion Electric heating performance

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Complete genome sequence of Bacillus amyloliquefaciens YP6, a plant growth rhizobacterium efficiently degrading a wide range of organophosphorus pesticides

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Journal of Integrative Agriculture 2019年第11期18卷 2668-2672页

作者： MENG Di ZHAI Li-xin TIAN Qiao-peng GUAN Zheng-bing CAI Yu-jie LIAO Xiang-ru key laboratory of industrial biotechnology Ministry of Education School of Biotechnology Jiangnan University

Bacillus amyloliquefaciens YP6,a plant growth promoting rhizobacteria,is capable of efficiently degrading a wide range of organophosphorus pesticides(OPs).Here,we report the complete genome sequence of this bacterium with a genome size of 4009619 bp,4210 protein-coding genes and an average GC content of 45.9%.Based on the genome sequence,several genes previously described as being involved in solubilizing-phosphorus,OPs-degradation,indole-3-acetic acid(IAA)and siderophores ***,compared with the genomes of *** species,strain YP6 had larger genome size and the most protein-coding ***,the four categories of“cell envelope biogenesis,outer membrane(M),”“translation,ribosomal structure and biogenesis(J),”“transcription(K),”and“signal transduction mechanisms(T)”were *** differences may be related to extensive environmental adaptability of the genus *** results expand the application potential of strain YP6 for environmental bioremediation,provide gene resources involved in OPs degradation for biotechnology and gene engineering,and contribute to provide insights into the relationship between microorganism and living environment.

关键词： organophosphorus pesticides genome sequence plant growth promoting rhizobacteria Bacillus amyloliquefaciens

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Evaluation of Bacillus sp. MZS10 for decolorizing Azure B dye and its decolorization mechanism

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Journal of Environmental Sciences 2014年第5期26卷 1125-1134页

作者： Huixing Li Ruijing Zhang Lei Tang Jianhua Zhang Zhonggui Mao The key laboratory of industrial biotechnology Ministry of Education School of Biotechnology Jiangnan University school of Biological and Chemical Engineering Nanyang Institute of Technology

To evaluate decolorization and detoxification of Azure B dye by a newly isolated Bacillus sp. MZS 10 strain, the cultivation medium and decolorization mechanism of the isolate were investigated. The decolorization was discovered to be dependent on cell density of the isolate and reached 93.55% （0.04 g/L） after 14 hr of cultivation in a 5 L stirred-tank fermenter at 2.0 g/L yeast extract and 6.0 g/L soluble starch and a small amount of mineral salts. The decolorization metabolites were identified with ultra performance liquid chromatography-tandem mass spectroscopy （UPLC-MS）. A mechanism for decolorization of Azure B was proposed as follows： the C=N in Azure B was initially reduced to -NH by nicotinamide adenine dinucleotide phosphate （NADPH）-dependent quinone dehydrogenase, and then the -NH further combined with -OH derived from glucose to form a stable and colorless compound through a dehydration reaction. The phytotoxicity was evaluated for both Azure B and its related derivatives produced by Bacillus sp. MZS 10 decolorization, indicating that the decolorization metabolites were less toxic than original dye. The decolorization efficiency and mechanism shown by Bacillus sp. MZS10 provided insight on its potential application for the bioremediation of the dye Azure B.

关键词： Azure B Bacillus sp. decolorization ultra performance liquid chromato graphy-tandem mass spectroscopy phytotoxicity

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Development of a new cleaner production process for cassava ethanol

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Chinese Journal of Chemical Engineering 2017年第4期25卷 493-498页

作者： Ke Wang Xinchao Yang Xidong Ren Jianhua Zhang Zhonggui Mao The key laboratory of industrial biotechnology Ministry of EducationSchool ofBiotechnologyJiangnan UniversityWuxi 214122China

A new cleaner production process for cassava ethanol has been developed, in which the thin stillage by-product was treated initially by anaerobic digestion, and the digestate further processed by hydrogen-form cation exchange resin before being recycled as process water to make mash for the next ethanol fermentation *** wastewater was eliminated and freshwater and energy consumption was significantly reduced. To evaluate the new process, ten consecutive batches of ethanol fermentation and anaerobic digestion at lab scale were carried out. Average ethanol production in the recycling batches was 11.43%(v/v) which was similar to the first batch, where deionized(DI) water was used as process water. The chemical oxygen demand(COD) removal rate reached 98% and the methane yield was 322 ml per gram of COD removed, suggesting an efficient and stable operation of the anaerobic digestion. In conclusion, the application of the new process can contribute to sustainable development of the cassava ethanol industry.

关键词： anaerobic fermentation digestion resin ethanol batch eliminated processed wastewater removed

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Time-resolved fluoroimmunoassay of zearalenone in cereals with a europium chelate as label

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Journal of Rare Earths 2009年第6期27卷 1088-1091页

作者：马智鸿黄飚张珏张艺朱岚屠蔷 Jiangsu Institute of Nuclear Medicine Key Laboratory of Nuclear MedicineMinistry of Health Department of Pharmaceutical and Molecular biotechnology School of BiotechnologyKey Laboratory of Industrial BiotechnologyMinistry of EducationSouthern Yangtze University

A competitive indirect time-resolved fluoroimmunoassay(TRFIA) was developed for detection of zearalenone(ZEN) in cereals,in which ZEN conjugated to bovine serum albumin(BSA) is used as solid-phase antigen.A competitive indirect TRFIA was conducted by simultaneously incubating ZEN in standard or extracted samples with anti-ZEN monoclonal antibody over ZEN-BSA coated plates,and then determining the bound ZEN monoclonal antibody with goat anti-mouse europium *** were extracted with methanol/water...

关键词： time-resolved fluoroimmunoassay zearalenone europium rare earths food safety

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Molecular characterization of bacterial community in aerobic granular sludge stressed by pentachlorophenol

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Journal of Environmental Sciences 2008年第10期20卷 1243-1249页

作者： LIU, He LI, Guangwei LI, Xiufen CHEN, Jian laboratory of Environmental biotechnology School of Biotechnology Jiangnan University Wuxi 214122 China key laboratory of industrial biotechnology Ministry of Education of China Jiangnan University Wuxi 214122 China

To characterize the effects of pentachlorophenol (PCP) on the performance and microbial community of aerobic granular sludge in sequencing batch reactor (SBR), the web-based terminal restriction fragment length polymorphism (T-RFLP) and real-time PCR (RT- PCR) techniques were used to explore the bacterial community structure. When PCP increased from 0 to 50 mg/L, the COD removal rate changed little, while the ammonia removal rate dropped from 100% to 64.9%. The results of molecular characterization showed t...

关键词： aerobic granular sludge bacterial community real-time PCR terminal restriction fragment length polymorphism sequencing batch reactor pentachlorophenol (PCP)

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Identification and expression analysis of bovine ANGPTL gene family

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Current Zoology 2010年第4期56卷 445-453页

作者： Zhengbing GUAN Guolin CAI Junyong SUN Jian LU key laboratory of industrial biotechnology Ministry of Education Jiangnan University Wuxi 214122 China school of biotechnology Jiangnan University Wuxi 214122 China

Encoded by seven genes, angiopoietin-like （ANGPTL） family members structurally similar to the angiogenic regulating factor angiopoietin are known to possess biological activities in angiogenesis and metabolism. Here we reports for the first time the identification and expression analysis of all the seven members of bovine ANGPTL gene family, which were designated bANGPTL1 to bANGPTL7 in order. The seven bANGPTL genes consist of 4-9 exons, span 3800M-3000 bp and are located on different chromosomes. The deduced amino acid sequences of the members all possess an N-terminal coiled-coil domain and a C-terminal fibrinogen-like domain, both characteristics of angiopoietins. Phylogenetic analysis showed that the 32 identified ANGPTL homologs from 9 species could be classified into two major groups. Real-time quantitative PCR （Q-PCR） analysis revealed that the bANGPTL family members have different expression patterns. This study will be helpful for investigation on the biological role of the bANGPTL family in this economically important species. Furthermore, it provides an insight into the molecular evolution of the emerging ANGPTL family

关键词： Cattle Angiopoietin-like Phylogenetic analysis Q-PCR

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A new photon kinetic-measurement based on the kinetics of electron-hole pairs in photodegradation of textile wastewater using the UV-H_2O_(2FS)-TiO_2 process

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Journal of Environmental Sciences 2007年第3期19卷 367-373页

作者： SHIGWEDHA Nditange HUA Zhao-zhe CHEN Jian laboratory of Environmental biotechnology School of Biotechnology Southern Yangtze University Wuxi 214036 China key laboratory of industrial biotechnology Ministry of Education Southern Yangtze University Wuxi 214036 China

Actual textile wastewater and synthesized wastewater containing various textile dyes were photocatalytic degraded by the UVH2O2Fs-TiO2 process in an aimular-flow photocatalytic reactor. In this process, a photon kinetic-measure was adopted to obtain constant rates of dyes decomposition. It was theorized that, by illumination at different UV frequencies, the electrons within the semiconductor were excited from the valence band to the conduction band, yielding the formation of electron-hole pairs which are the pre-requisites for photocatalysis. CPT （critical photonic time） exposure required to cause 90% of vibrations between the double and single bonds along the molecular chain of the dyes to be oxidized, was taken to measure the photocatalytic activities. The CPTs varied with the frequencies of the UV spectral areas. The derivatization of CPT from the first-order kinetic law was presented.

关键词： critical photonic time (CPT) photon efficiency UV frequencies regression line textile wastewater

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Reconstruction of tyrosol synthetic pathways in Escherichia coli

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Chinese Journal of Chemical Engineering 2018年第12期26卷 2615-2621页

作者： Cui Yang Xianzhong Chen Junzhuang Chang Lihua Zhang Wei Xu Wei Shen You Fan key laboratory of Carbohydrate Chemistry & biotechnology Ministry of Education Jiangnan University school of biotechnology Jiangnan University

Tyrosol is a pharmacologically active phenolic compound widely used in the medicine and chemical *** methods of plant extraction are complicated and chemical synthesis of tyrosol is not commercially viable. In this study, a recombinant Escherichia coli strain was constructed by overexpressing the phenylpyruvate decarboxylase ARO10 from Saccharomyces cerevisiae, which could produce tyrosol from glucose. Furthermore,genes encoding key enzymes from the competing phenylalanine and tyrosine synthesis pathways and the repression protein TyrR were eliminated, and the resulting engineered strain generated 3.57 mmol·L^(-1) tyrosol from glucose. More significantly, codon optimization of ARO10 increased expression and tyrosol titer. Using the novel engineered strain expressing codon-optimized AR10 in shake-flask culture, 8.72 mmol·L^(-1) tyrosol was obtained after 48 h. Optimization of the induction conditions improved tyrosol production to 9.53 mmol·L^(-1)(1316.3 mg·L^(-1)). A higher titer of tyrosol was achieved by reconstruction of tyrosol synthetic pathway in E. coli.

关键词： Tyrosol Escherichia coli Phenylpyruvate decarboxylase Gene knockout Codon optimization

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