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Quantifying muskmelon fruit attributes with A-TEP-based model and machine vision measurement

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Journal of Integrative agriculture 2018年第6期17卷 1369-1379页

作者： CHANG Li-ying HE San-peng LIU Qian XIANG Jia-lin HUANG Dan-feng School of Agriculture and Biology Shanghai Jiaotong University

In this study, we established a dynamic morphological model using the accumulated thermal effectiveness and photosynthetic active radiation （PAR）（A-TEP）, aiming to explore the relationship between muskmelon （Cucumis melo L.） fruit attributes and environmental factors. Muskmelon surface color was described by parameters of red, green, blue, hue, saturation and brightness （HSI）. Three characteristic parameters, gray level co-occurrence matrix （GLCM）, angular second moment （ASM）, entropy, contrast, and the coverage rate were used to describe the process of muskmelon fruit netting formation. ASM was not significant difference during muskmelon fruit growth. The number and deep of netting stripes gradually increased with fruit growth. Coverage rate increased rapidly for 15-30 d after pollination. The vertical and horizontal diameters of muskmelon fruit were followed a logistic curve. And root mean squared errors （RMSE） between the simulated and measured vertical and horizontal diameters were 3.527 and 4.696 mm, respectively. RMSE of red, green, blue, saturation and brightness were 0.999, 2.690, 2.992, 0.033 and 5.51, respectively, and the RMSE for entropy, contrast and coverage rates were 0.077, 0.063 and 0.015, respectively, indicating a well consistent between measured and simulated values.

关键词： machine vision technology fruit attributes A-TEP skin netting coverage rate

Determination of trans-4-hydroxy-2-alkenals in thermally treated soybean oil by SPE-HPLC

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Grain & Oil Science and Technology 2022年第3期5卷 107-113页

作者： Hongying Hua Shimin Wu Xin Ma School of Agriculture and Biology Shanghai Jiao Tong UniversityShanghai 200240China

Trans-4-hydroxy-2-hexenal(4-HHE) and trans-4-hydroxy-2-nonenal(4-HNE) are secondary lipid peroxidation products in edible oils, which are cytotoxic and genotoxic. They could covalently bind with protein, phospholipids and DNA, further disrupting the normal function of liver, lung and brain.Derivation process was generally conducted during pretreatment before detection and quantification of 4-HHE and 4-HNE. However, the derivation procedures were time consuming and chemical degradation may occur during the process. Hence, this paper aims to establish a simple solid phase extractionhigh performance liquid chromatography(SPE-HPLC) method to determine the 4-HHE and 4-HNE contents in thermally treated soybean oil. C18 solid phase extraction was applied in the pretreatment process. Firstly, the reliability of the method was evaluated. Good linearity was observed in the range of 0.1–0.5 μg/m L and 0.5–10 μg/m L for 4-HHE and 4-HNE. The limit of detection(LOD) of 4-HHE and 4-HNE were 0.0486 and 0.0129 μg/m L, respectively. And the limit of quantitation(LOQ) of4-HHE and 4-HNE were 0.1458 and 0.0431 μg/m L, respectively. Recovery rate were in the range of89.11%–91.58% and 71.83%–79.40% for 4-HHE and 4-HNE, respectively. The method achieved the extraction, purification and detection of 4-HHE and 4-HNE simultaneously and had the advantages of simple operation, effectiveness, high precision, good repeatability. Then, the method was applied to monitor the concentrations of 4-HHE and 4-HNE in soybean oil heated at 180 °C for 40 h. The contents of 4-HHE and 4-HNE were 0–0.32 μg/g and 0–6.97 μg/g, respectively, which provided guidance for evaluating health risks of thermally treated soybean oil during heating.

关键词： Trans-4-hydroxy-2-hexenal Trans-4-hydroxy-2-nonenal Soybean oil

Overexpression of G10-EPSPS in soybean provides high glyphosate tolerance

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Journal of Integrative agriculture 2019年第8期18卷 1851-1858页

作者： XIAO Pei-ying LIU Yi CAO Yue-ping School of Agriculture and Biology Shanghai Jiao Tong University

Glyphosate is a highly efficient, broad-spectrum nonspecific herbicide that inhibits the 5-enolpyruvylshikimate-3-phosphate synthase(EPSPS)-mediated pathway of shikimic acid. The screening of glyphosate-resistant EPSPS gene is a major means for the development of new genetically modified glyphosate-resistant transgenic crop. Currently, the main commercialized glyphosate-resistant soybean contains glyphosate-resistant gene CP4-EPSPS. In this study, a G10-EPSPS gene was reported providing glyphosate resistance in Zhongdou 32. Here, G10-EPSPS gene was introduced into soybeans through Agrobacterium-mediated soybean cotyledon node. PCR, Southern blotting, semi-quantitative RT-PCR, qRT-PCR, and Western blotting were used, and the results revealed that G10-EPSPS had been integrated into the soybean genome and could be expressed steadily at both mRNA and protein levels. In addition, glyphosate resistance analysis showed that the growth of transgenic soybean had not been affected by concentrations of 900 and 2 700 g a.e. ha–1 of glyphosate. All the results indicated that G10-EPSPS could provide high glyphosate resistance in soybeans and be applied in production of glyphosate-resistant soybean.

关键词： glyphosate transgenic soybean G10-EPSPS glyphosate-resistant

Micro-trichome as a class I homeodomain-leucine zipper gene regulates multicellular trichome development in Cucumis sativus

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Journal of Integrative Plant biology 2015年第11期57卷 925-935页

作者： Jun-Long Zhao Jun-Song Pan Yuan Guan Wei-Wei Zhang Bei-Bei Bie Yun-Li Wang Huan-Le He Hong-Li Lian Run Cai School of Agriculture and Biology Shanghai Jiao Tong University

Plant trichomes serve as a highly suitable model for investigating cell differentiation at the single-cell level. The regulatory genes involved in unicellular trichome develop- ment in Arabidopsis thaliana have been intensively studied, but genes regulating multicellular trichome development in plants remain unclear. Here, we characterized Cucumis sativus （cucumber） trichomes as representative multicellular and unbranched structures, and identified Micro-trichome （Mict）, using map-based cloning in an F2 segregating population of 7,936 individuals generated from a spontaneous mict mutant. In mitt plants, trichomes in both leaves and fruits, are small, poorly developed, and denser than in the wild type. Sequence analysis revealed that a 2,649-bp genomic deletion, spanning the first and second exons, occurred in a plant-specific class I homeodomain-leucine zipper gene. Tissue-specific expression analysis indicated that Mict is strongly expressed in the trichome cells. Transcriptome profiling identified potential targets of Mict including putative homologs of genes known in other systems to regulate trichome development, meristem determinacy, and hormone responsiveness. Phylo- genic analysis charted the relationships among putative homologs in angiosperms. Our paper represents initial steps toward understanding the development of multicellular trichomes.

关键词： Cucumis sativus HD-Zip gene map-based cloning multicellular trichome transcriptome

Functional Characterization of SmbHLH13 in Anthocyanin Biosynthesis and Flowering in Eggplant

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Horticultural Plant Journal 2021年第1期7卷 73-80页

作者： Haochun Xi Yongjun He Huoying Chen School of Agriculture and Biology Shanghai JiaoTong UniversityShanghai 200240China

Anthocyanin is abundant in a few vegetables,including eggplant.It protects plants from abiotic stress and benefits human health,making the research of anthocyanin biosynthesis increasingly important.Flowering time is an important reference for judging reproduction and adaptability,which can guide plant production.In this study,SmbHLH13 from eggplant was identified.Yeast one-hybrid and dual-luciferase assays showed that SmbHLH13 binded and activated the expression of structural genes SmCHS and SmF3H in anthocyanin biosynthesis and it also was bound to the promoter of the key gene SmFT in flowering.Furthermore,genetic transformation of Arabidopsis revealed that overexpression of SmbHLH13 enhanced anthocyanin accumulation and delayed flowering.These results demonstrated that SmbHLH13 might promote anthocyanin accumulation through positive regulation of SmCHS and SmF3H.Moreover,SmbHLH13 might have a role in delaying eggplant flowering.

关键词： eggplant Solanum melongena transcription factor anthocyanin biosynthesis flowering

Inhalation of Cananga odorata essential oil relieves anxiety behaviors in autism-like rats via regulation of serotonin and dopamine metabolism

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Journal of Integrative Medicine 2023年第2期21卷 205-214页

作者： Nan Zhang Shu-ting Wang Lei Yao school of Design Shanghai Jiao Tong UniversityShanghai 200000China School of Agriculture and Biology Shanghai Jiao Tong UniversityShanghai 200000China

Objective:Anxiety is one of the most common symptoms associated with autistic spectrum disorder.The essential oil of Cananga odorata(Lam.)Hook.f.&Thomson,usually known as ylang-ylang oil(YYO),is often used in aromatherapy as a mood-regulating agent,sedative,or hypotensive agent.In the present study,the effects and mechanisms of YYO in alleviating anxiety,social and cognitive behaviors in autism-like rats were investigated.Methods:The prenatal valproic acid(VPA)model was used to induce autism-like behaviors in offspring rats.The effectiveness of prenatal sodium valproate treatment(600 mg/kg)on offspring was shown by postnatal growth observation,and negative geotaxis,olfactory discrimination and Morris water maze(MWM)tests.Then three treatment groups were formed with varying exposure to atomized YYO to explore the effects of YYO on the anxiety,social and cognitive behaviors of the autistic-like offspring through the elevated plus-maze test,three-chamber social test,and MWM test.Finally,the monoamine neurotransmitters,including serotonin,dopamine and their metabolites,in the hippocampus and prefrontal cortex(PFC)of the rats were measured using a high-performance liquid chromatography.Results:Offspring of VPA exposure rats showed autism-like behaviors.In the VPA offspring,mediumdose YYO exposure significantly elevated the time and entries into the open arms in the elevated plusmaze test,while low-dose YYO exposure significantly enhanced the social interaction time with the stranger rat in session 1 of the three-chamber social test.VPA offspring treated with YYO exposure used less time to reach the platform in the navigation test of the MWM test.YYO exposure significantly elevated the metabolism of serotonin and dopamine in the PFC of VPA offspring.Conclusion:YYO exposure showed the effects in alleviating anxiety and improving cognitive and social abilities in the offspring of VPA exposure rats.The role of YYO was related to the regulation of the metabolism of serotonin and dopamine.

关键词： Cananga odorata essential oil autism-like rat anxiety social behavior monoamine neurotransmitter

基于数据独立采集的磷酸化蛋白质组学全面揭示I型酪蛋白激酶在植物发育中的作用

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科学通报:英文版 2023年第18期68卷 2077-2093,M0004页

作者：渠莉刘默洋郑玲丽王旭薛红卫 Shanghai Collaborative Innovation Center of Agri-Seeds Joint Center for Single Cell BiologySchool of Agriculture and BiologyShanghai Jiao Tong UniversityShanghai 200240China

I型酪蛋白激酶(CKI)是一类真核生物中高度保守的丝氨酸/苏氨酸蛋白激酶,通过介导底物的磷酸化调节生长发育和信号转导:拟南芥蛋白AELs,是植物特异的CK1,具有多种功能,但其内源底物的准确识别和验证极大地限制了对CK1生理功能的系统阐明... 详细信息

I型酪蛋白激酶(CKI)是一类真核生物中高度保守的丝氨酸/苏氨酸蛋白激酶,通过介导底物的磷酸化调节生长发育和信号转导:拟南芥蛋白AELs,是植物特异的CK1,具有多种功能,但其内源底物的准确识别和验证极大地限制了对CK1生理功能的系统阐明.本研究通过数据独立采集模式(DIA)开展的磷酸化蛋白质组学研究系统揭示了CKI的内源底物:从AELs过表达及多种突变体的幼苗中提取蛋白并进行质谱检测,与野生型比较分析获取了3985个在AELs过表达材料中丰度高于野生型(Col-0)以及在缺失突变体中丰度低于Col-0的磷酸化肽段(AEL依赖的磷酸化肽段).对应获得1032个磷酸化蛋白。进一步从磷酸化肽段中富集了8个底物识别基序并对其中4个新基序进行了实验验证,用于预测CK1的潜在候选底物,基于富集到的一个参与生殖发育的锌指转录因子,C3H17,我们通过生化和遗传分析进行了底物验证和功能鉴定,证明AEL通过介导C3H17磷酸化以增强蛋白稳定性和转录活性进而调控拟南芥的胚胎发育,基于CK1在真核生物中的高度保守性,进一步利用CK1的底物识别基序搜索了水稻、小鼠和人类的蛋白数据库,获得了远超已知数目的众多候选底物,极大地扩展了对CK1在动植物中发挥的共同和独特功能的理解,为跨物种全面揭示CK1的保守功能提供了重要线索.

关键词： Casein kinase 1(CK1) Arabidopsis EL1-like(AEL) Data-independent acquisition(DIA) Phosphoproteomics C3H17 Embryo development

The small and large subunits of carbamoyl-phosphate synthase exhibit diverse contributions to pathogenicity in Xanthomonas citri subsp.citri

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Journal of Integrative agriculture 2015年第7期14卷 1338-1347页

作者： GUO Jing SONG Xue ZOU Li-fang ZOU Hua-song CHEN Gong-you School of Agriculture and Biology Shanghai Jiao Tong University

Carbamoyl-phosphate synthase plays a vital role in the carbon and nitrogen metabolism cycles. In Xanthomonas citrisubsp. citri, carA and carB encode the small and large subunits of carbamoyl-phosphate synthase, respectively. The deletion mutation of the coding regions revealed that carA did not affect any of the phenotypes, while carB played multiple roles in pathogenicity. The deletion of carB rendered the loss of pathogenicity in host plants and the ability to induce a hyper- sensitive reaction in the non-hosts. Quantitative reverse transcription-PCR assays indicated that 11 hrp genes coding the type Ill secretion system were suppressed when interacting with citrus plants. The mutation in carB also affected bacterial utilization of several carbon and nitrogen resources in minimal medium MMX and extracellular enzyme activities. These data demonstrated that only the large subunit of carbamoyl-phosphate synthase was essential for canker development by X. citri subsp, citri.

关键词： Xanthomonas citri subsp citri carbamoyl-phosphate synthase pathogenicity citrus canker hrp gene

Genomic and transcriptomic analyses reveal metabolic complementarity between whiteflies and their symbionts

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Insect Science 2022年第2期29卷 539-549页

作者： Dan-Tong Zhu Qiong Rao Chi Zou Fei-Xue Ban Juan-Juan Zhao Shu-Sheng Liu Ministry of agriculture Key Lab of Molecular biology of Crop Pathogens and Insects Institute of Insect SciencesZhejiang UniversityHangzhou310029 China school of agriculture and Food Science Zhejiang Agriculture and Forestry UniversityLin'anHangzhou311300 China

Nutritional mutualism between insects and symbiotic bacteria is widespread.The various sap-feeding whitefly species within the Bemisia tabaci complex associate with the same obligate symbiont(Portiera)and multiple secondary symbionts.It is often assumed that some of the symbionts residing in the whiteflies play crucial roles in the nutritional physiology of their insect hosts.Although effort has been made to understand the functions of the whitefly symbionts,the metabolic complementarity offered by these symbionts to the hosts is not yet well understood.We examined two secondary symbionts,Arsenophonus and Wolbachia,in two species of the B.tabaci whitefly complex,provisionally named as Asia II 3 and China 1.Genomic sequence analyses revealed that Arsenophonus and Wolbachia retained genes responsible for the biosynthesis of B vitamins.We then conducted transcriptomic surveys of the bacteriomes in these two species of whiteflies together with that in another species named MED of this whitefly complex previously reported.The analyses indicated that several key genes in B vitamin syntheses from the three whitefly species were identical.Our findings suggest that,similar to another secondary symbiont Hamiltonella,Arsenophonus and Wolbachia function in the nutrient provision of host whiteflies.Although phylogenetically distant species of symbionts are associated with their respective hosts,they have evolved and retained similar functions in biosynthesis of some B vitamins.Such metabolic complementarity between whiteflies and symbionts represents an important feature of their coevolution.

关键词： symbiont B vitamins complement evolution whitefly