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LSQP‑DB:a species‑specific quantitative PCR primer database for 307 Lactobacillaceae species

Systems Microbiology and Biomanufacturing 2023年第4期3卷 593-601页

作者： Rubing Du Shilei Wang Qun Wu Yan Xu Lab of brewing Microbiology and Applied Enzymology State Key Laboratory of Food Science and TechnologySynergetic Innovation Center of Food Safety and NutritionSchool of BiotechnologyJiangnan UniversityWuxi 214122JiangsuChina

Quantitative real-time PCR is widely used to determine absolute abundance of microbes in food ***,it remains challenges in the application for quantification at the species level due to the difficulty in designing species-specific primer *** work,using Lactobacillaceae,a dominant family within the lactic acid bacteria that involved in diversity food fermentations,as a case,presents an extendable strategy to design species-specific primer sets for microbial quantita-tive analysis.136,257 species-specific genes were obtained from all 307 species within Lactobacillaceae family through comparative genomics analysis.A total of 130,521 primer sets were designed using species-specific *** them,81,710 primer sets had 100%interspecific specificity and 100%intraspecific coverage,and were reserved to quantify all 307 individual Lactobacillaceae *** primer sets had uniform melting temperature(57–63℃)and product size(100–300 bp),that allowed simultaneously quantify different Lactobacillaceae species with the same qPCR *** then established a Lactobacillaceae species quantitation primer database(LSQP-DB,http://***)containing all 81,710 species-specific primer *** database would facilitate a fast and easy absolute quantitation analysis of all indi-vidual Lactobacillaceae *** work represented the first ever large-scale integration of species-specific primer sets for microorganism,it can be extended to other bacterial and fungal genera to advance development of microbial absolute quantification.

关键词： Lactobacillaceae Primer database Species-specific primer qPCR

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Directional design and synthesis of high-yield hollow Fe-MFI zeolite encapsulating ultra-small Fe_(2)O_(3) nanoparticles by using mother liquid

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Nano Research 2021年第11期14卷 4304-4313页

作者： Yi Zhai Fumin Wang Xubin Zhang Guojun Lv Yuzhou Wu Tao Jiang Qing Zhang Mengyue Li Mengyao Li Yongkui Liu school of Chemical engineering and Technology Tianjin UniversityTianjin300350China school of Environmental and Chemical engineering Jiangsu University of Science and TechnologyZhenjiang212003China

How to directionally design the hollow zeolite via a green route is of great significance. Here, we successfully synthesized the hollow Fe-silicate-1 encapsulated ultra-small Fe_(2)O_(3) nanoparticles (2.5 nm) with higher yield (85.2%) by mother liquid than traditional dissolution-recrystallization for the first time, which was achieved by precisely regulating the number and distribution of defects in zeolite and cleverly utilizing the TPAOH and nuclei in mother liquor. The effects of synthetic temperature, synthetic period and addition amount of parent zeolite on the formation of hollow zeolite have been investigated and the effect of synthetic conditions on the defects in parent zeolite has been also firstly quantified. The corresponding formation mechanism has been proposed. The abundant inner defects provided by the zeolite synthesized at 130 °C for 1 day and large amount of TPAOH remaining in mother liquid are conducive to the formation of hollow zeolite. Meanwhile, both parent zeolite and nuclei (4-, 5-member rings and structure units) in mother liquid obtained at 130 °C play the crucial roles in enhancing the zeolite yield. Notably, Fe_(2)O_(3) nanoparticles could decompose into small fragments by the interaction with nuclei in mother liquid. Partial ultra-small Fe_(2)O_(3) nanoparticles would be encapsulated in cavity and the rest could be inserted in the zeolite framework, which is significantly different from the conventional dissolution-recrystallization mechanism. The obtained encapsulated catalyst shows the superior catalytic performance and stability in phenol and tetracycline degradation reactions.

关键词： mother liquid hollow zeolite high yield defect sites degradation reaction

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Growth properties of mixed liquor suspended solids in SMBR for pharmaceutical wastewater treatment

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Journal of Harbin Institute of Technology(New Series) 2010年第2期17卷 171-175页

作者：祁佩时李正陈兆波 school of Municipal and Environmental engineering Harbin Institute of Technology school of Material Science and Chemical engineering Harbin Engineering University

This paper presents the performance results of a 366-day pilot-scale submerged membrane bioreactor (SMBR) for treating high-strength pharmaceutical wastewater. The study focuses on the growth properties of mixed liquor suspended solids (MLSS) in SMBR operated at high volumetric loading rates. The influences of MLSS on COD removal,sludge yield,oxygen utilization rates and sludge viscosity are studied. Results show that the bioreactor can be operated at higher volumetric loading rate with a low sludge yield. VSS/SS and observed sludge yield coefficient (Yo) present a decreasing trend with the decrease of hydraulic retention time (HRT) . Sludge oxygen utilization rate decreases with the increase of mixed liquor volatile suspended solids (MLVSS) . A mathematical model between sludge viscosity and MLSS is developed.

关键词： submerged membrane bioreaetor (SMBR) pharmaceutical wastewater mixed liquor suspended solids

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Production of high-purity hydrogen from paper recycling black liquor via sorption enhanced steam reforming

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Green Energy & Environment 2021年第5期6卷 771-779页

作者： Hanke Li Shijie Wu Chengxiong Dang Guangxing Yang Yonghai Cao Hongjuan Wang Feng Peng Hao Yu State Key Laboratory of Pulp and Paper engineering School of Chemistry and Chemical EngineeringSouth China University of TechnologyGuangzhou510640China school of Chemistry and Chemical engineering Guangzhou UniversityGuangzhou510006China

Environmentally friendly and energy saving treatment of black liquor(BL),a massively produced waste in Kraft papermaking process,still remains a big ***,by adopting a NieCaOeCa_(12)Al_(14)O_(33) bifunctional catalyst derived from hydrotalcite-like materials,we demonstrate the feasibility of producing high-purity H_(2)(~96%)with 0.9 mol H_(2) mol^(-1) C yield via the sorption enhanced steam reforming(SESR)of *** SESRBL performance in terms of H_(2) production maintained stable for 5 cycles,but declined from the 6th ***,Raman spectroscopy,elemental analysis and energy dispersive techniques were employed to rationalize the deactivation of the *** was revealed that gradual sintering and agglomeration of Ni and CaO and associated coking played important roles in catalyst deactivation and performance degradation of SESRBL,while deposition of Na and K from the BL might also be responsible for the declined *** the other hand,it was demonstrated that the SESRBL process could effectively reduce the emission of sulfur species by storing it as CaSO_(3).Our results highlight a promising alternative for BL treatment and H_(2) production,thereby being beneficial for pollution control and environment governance in the context of mitigation of climate change.

关键词： Black liquor High-purity hydrogen Sorption enhanced steam reforming Sulfur removal

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Hepatoprotective ability of tetramethylpyrazine produced by Bacillus amyloliquefaciens

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Systems Microbiology and Biomanufacturing 2021年第2期1卷 223-233页

作者： Wenqing Zhang Guanru Si Zhiming Rao Shuai Zong Jinglei Li Xian Zhang Chuanqiang Gao Zhou Ping Ming Ye school of Food and Biological engineering Hefei University of Technology230009 HefeiChina Research Institute of Jiangnan Small Pit brewing Technology Xuancheng 242000China The Key Laboratory of Industrial Biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi 214122JiangsuChina College of Food Science and engineering Yangzhou UniversityYangzhou 225127JiangsuChina

Tetramethylpyrazine(TTMP),an important aroma compound,was produced by Bacillus amyloliquefaciens XJB-104 with distillers’grains as raw *** yield of TTMP under optimized fermentation procedure was 3176.52 mg/*** in the fermentation broth was purified and the purity(>99%)was measured by gas chromatography-mass ***,the hepatoprotective activity of TTMP in ethanol-water system was evaluated by biochemical indicators of liver injury,parameters of antioxidant defense system and inflammatory response,and liver histopathological assessment in *** treatment increased serum levels of alanine aminotransferase,aspartate aminotransferase,alkaline phos-phatase and lactate dehydrogenase,suggesting liver *** high and low dose of TTMP significantly decreased levels of these ***,the reduction in the activities or concentrations of superoxide dismutase,catalase,reduced glutathione and malondialdehyde in liver tissue caused by ethanol was significantly alleviated by *** inflammatory cytokines including transcription factor,tumor necrosis factor,interleukin-1beta,interleukin,macrophage chemoattractant protein,inducible nitric oxide synthase and cyclooxygenase were also suppressed by TTMP *** is concluded that TTMP in ethanol-water system has potential liver-protective activity,especially when ethanol is consumed at low doses for short periods of time.

关键词： Tetramethylpyrazine Bacillus amyloliquefaciens Hepatoprotective effect Distillers’grains Ethanol-water system

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Preparation and characterization of PTFE coating in new polymer quartz piezoelectric crystal sensor for testing liquor products

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Chinese Physics B 2015年第7期24卷 544-547页

作者：谷宇李强 Department of Intelligence Science and Technology School of Automation and Electrical EngineeringUniversity of Science & Technology Beijing Department of Mechanics School of Civil EngineeringBeijing Jiaotong University

A new method was developed based on the electron beam vacuum dispersion（EBVD） technology to prepare the PTFE polymer coating of the new polymer quartz piezoelectric crystal sensor for testing liquor products. The new method was applied in the new EBVD equipment which we designed. A real-time system monitoring the polymer coating＇s thickness was designed for the new EBVD equipment according to the quartz crystal microbalance（QCM） principle, playing an important role in preparing stable and uniform PTFE polymer coatings of the same thickness. 30 pieces of PTFE polymer coatings on the surface of the quartz crystal basis were prepared with the PTFE polymer ultrafine powder（purity ≥ 99.99%）as the starting material. We obtained 30 pieces of new PTFE polymer sensors. By using scanning electron microscopy（SEM）, the structure of the PTFE polymer coating＇s column clusters was studied. One sample from the 30 pieces of new PTFE polymer sensors was analysed by SEM in four scales, i.e., 400×, 1000×, 10000×, and 25000×. It was shown that under the condition of high bias voltage and low bias current, uniformly PTFE polymer coating could be achieved, which indicates that the new EBVD equipment is suitable for mass production of stable and uniform polymer coating.

关键词： PTFE coating electron beam vacuum dispersion mass production of polymer coating

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Effect of powdered activated carbon on Chinese traditional medicine wastewater treatment in submerged membrane bioreactor with electronic control backwashing

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Journal of Environmental Sciences 2007年第9期19卷 1037-1042页

作者： LIU Xiao-lei REN Nan-qi MA Fang school of Municipal and Environmental engineering Harbin Institute of Technology Harbin 150090 China. school of Water Conservancy and Environment engineering Changchun Institute of Technology Changchun 130012 China

Chinese traditional medicine wastewater, rich in macromolecule and easy to foam in aerobic biodegradation such as Glycosides, was treated by two identical bench-scale aerobic submerged membrane bioreactors （SMBRs） operated in parallel under the same feed, equipped with the same electronic control backwashing device. One was used as the control SMBR （CSMBR） while the other was dosed with powdered activated carbon （PAC）（PAC-amended SMBR, PSMBR）. The backwashing interval was 5 min. One suction period was about 90 min by adjusting preestablished backwashing vacuum and pump frequency. The average flux of CSMBR during a steady periodic state of 24 d （576 h） was 5.87 L/h with average hydraulic residence time （HRT） of 5.97 h and that of PSMBR during a steady periodic state of 30 d （720 h） was 5.85 L/h with average HRT of 5.99 h. The average total chemical oxygen demand （COD） removal efficiency of CSMBR was 89.29% with average organic loading rate （OLR） at 4.16 kg COD/（m^3.d） while that of PSMBR was 89.79% with average OLR at 5.50 kg COD/（m^3.d）. COD concentration in the effluent of both SMBRs achieved the second level of the general wastewater effluent standard GB8978-1996 for the raw medicine material industry （300 mg/L）. Hence, SMBR with electronic control backwashing was a viable process for medium-strength Chinese traditional medicine wastewater treatment. Moreover, the increasing rates of preestablished backwashing vacuum, pump frequency, and vacuum and flux loss caused by mixed liquor in PSMBR all lagged compared to those in CSMBR; thus the actual operating time of the PSMBR system without membrane cleaning was extended by up to 1.25 times in contrast with the CSMBR system, and the average total COD removal efficiency of PSMBR was enhanced with higher average OLR.

关键词： electronic control backwashing powdered activated carbon membrane bioreactor aerobic process wastewater treatment

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Effects of CFB Ash on the Adsorption Mechanism of Polycarboxylate Superplasticiser

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Journal of Wuhan University of Technology(Materials Science) 2018年第6期33卷 1323-1333页

作者： LIU Juanhong GAO Meng SONG Shaomin school of Civil and Resource engineering University of Science and Technology Beijing Beijing Key Laboratory of Urban Underground Space engineering College of Civil engineering and Architecture Henan University of Technology school of Civil and Transportation engineering Beijing University of Civil Engineering and Architecture

The effects of circulating fluid bed(CFB) ash on the adsorption performance of polycarboxylate superplasticiser and the mechanism of this influence on the dispersive property of the polycarboxylate superplasticiser were investigated by determing the cement paste fluidity, total organic carbon adsorption, infrared spectroscopic analyses and ζ potential test. The experimental results show that the addition of an inorganic salt into the mixture to change the content of SO42-and Fe2O3can improve the adaptability between the CFB ash and polycarboxylate superplasticiser. Adsorption may occur between the polycarboxylate superplasiciser and Fe2O3, SO42-or other components in CFB ash, leading to a significant reduction in paste fluidity. As the content of Na2SO4in CFB ash reaches 3% or Fe2O3reaches 9%, the paste loses its liquidity. The organic carbon content in the liquor decreases with an increase in Na2SO4or Fe2O3content. Adding some Ba(NO3)2and Na2S to the liquor can recover the organic carbon content to a certain extent, and the absolute value of ζ potential will increase. The addition of Ba(-NO3)2or Na2S reduces the adsorption property of Na2SO4or Fe2O3in CFB ash on the polycarboxylate superplasticiser.

关键词： CFB ash polycarboxylate superplasticiser fluidity TOC adsorption

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Thermal decomposition behaviour of polyacrylamidomethyltrimethyl ammonium chloride in red mud separation process

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Journal of Central South University of Technology 2008年第6期15卷 808-813页

作者：胡慧萍张琨瑜张丽娟陈启元 school of Chemistry and Chemical engineering Central South University

In order to provide references for selecting highly efficient red mud flocculants, the behaviour of polyacrylamidomethyltrimethyl ammonium chloride （PATAC） in red mud separation process was investigated. PATAC was employed as a flocculant for red mud separation from the caustic aluminate liquor at 95 ℃. The used red mud was generated from Chinese diaspore bauxite in Bayer process of alumina production. And the changes of PATAC before or after being treated in caustic solution at 95 ℃ were studied by thermogravimetry （TG） and Fourier transform infrared （FTIR） spectral analysis. The results show that PATAC fails in effectively focculating red mud and PATAC is readily converted to a quaternary ammonium hydroxide （PATAH） in caustic solution. PATAH can be decomposed to a new polymer （HPATAH） even at 95 ℃. Furthermore, there is an intramolecular hydrogen bond formed in the HPATAH polymer chain with two functional groups of--CH2--OH and --CONH2. Therefore, the poor flocculation property of PATAC for red mud separation can be attributed to the thermal decomposition of PATAC in the caustic red mud slurry at 95 ℃ and the formation of intramolecular hydrogen bond in the polymer chain of HPATAH during the thermal decomposition, which causes the absorbable functional groups of PATAC to decrease greatly.

关键词： alumina red mud polyacrylamidomethyltrimethyl ammonium chloride (PATAC) settlement thermal decomposition intramolecular hydrogen bond

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Study of decomposing carbonyl slag

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中国有色金属学会会刊：英文版 2006年第2期16卷 477-482页

作者：陈爱良孙培梅赵中伟李洪桂陈星宇 school of Metallurgical Science and engineering Central South University Changsha 410083 China school of Chemical engineering and Technology China University of Mining and Technology Beijing 100 083 China

A new technology was put forward to deal with the carbonyl slag at low acidity and low oxygen pressure in the kettle. With the orthogonal experiments for analyzing the sequence of four factors and some single factor experiments for the best conditions. The best conditions are used for extracting nickel, cobalt and copper and enriching precious metals: the cupric ion concentration is 5 g/L; and pH=6; the sulfur coefficient is 1.4; the oxygen pressure is 0.08 MPa; the time bubbling oxygen is 20 min; the ratio of liquid to solid is 8:1; the leaching time is 2 h; the heating time is 2.5 h. The leaching rates of nickel and cobalt are more than 98% and that of copper is above 97%. Nickel and cobalt can be separated efficiently from copper and precious metals from the carbonyl slag. Moreover, its leaching liquor has less copper. Nickel and cobalt can be reclaimed only once. During the whole process, the leaching rates of Au and Ag are more than 99.9%, while other precious metals are still in the residue without any loss.

关键词：羰基矿渣分解浸出有色金属综合利用

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