检索结果-南通市图书馆

Evaluation of anti-tubercular activity of linolenic acid and conjugatedlinoleic acid as effective inhibitors against Mycobacterium tuberculosis

引用

Asian Pacific Journal of Tropical Medicine 2016年第2期9卷 121-125页

作者： Won Hyung Choi Department of biomedical science and Department of Medical Zoology Kyung Hee University School of Medicine

Objective: To evaluate a new pharmacological activity/effect of linolenic acid(α- and γ-form) and conjugated-linoleic acid(CLA) causing antibacterial activity against Mycobacterium tuberculosis(Mtb). Methods: The anti-Mtb activity/effect of linolenic acid and CLA were determined using different anti-Mtb indicator methods such as resazurin microtiter assay(REMA) and MGIT 960 system assay. The Mtb was incubated with various concentrations(12.5–200) μg/m L of the compounds and anti-Mtb first-line drugs for 5 d in the REMA, and for 3 wk in MGIT 960 system assay. Results: Linolenic acid and CLA obviously indicated their anti-Mtb activity/effect by strongly inhibiting the growth/proliferation of Mtb in a dosedependent manner in the REMA and the MGIT 960 system assay. Interestingly, linolenic acid and CLA consistently induced anti-Mtb activity/effect by effectively inhibiting the growth/proliferation of Mtb in MGIT 960 system for 21 d with a single-treatment, and their minimum inhibitory concentrations were measured as 200 μg/m L respectively. Conclusions: These results demonstrate that linolenic acid and CLA not only have effective anti-Mtb activity/properties, but also induce the selective-anti-Mtb effects by strongly inhibiting and blocking the growth/proliferation of Mtb through a new pharmacological activity/action. Therefore, this study provides novel perspectives for the effective use of them and the potential that can be used as potent anti-Mtb candidate drugs, as well as suggests the advantage of reducing the cost and/or time for developing a new/substantive drug by effectively repurposing the existing drugs or compounds as one of new strategies for the global challenge of tuberculosis.

关键词： MGIT 960 system Tuberculosis Drug susceptibility testing γ-linolenic acid

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Rethinking resolution estimation in fluorescence microscopy: from theoretical resolution criteria to super-resolution microscopy

引用

science China(Life sciences) 2020年第12期63卷 1776-1785页

作者： Mengting Li Zhen-Li Huang Britton Chance Center for biomedical Photonics.Wuhan National Laboratory for Optoelectronics-Huazhong University of science and Technology Wuhan 430074.China MoE Key Laboratory for biomedical Photonics Collaborative Innovation Center for Biomedical Engineering.School of Engineering Sciences.Huazhong University of Science and TechnologyWuhan 430074China school of biomedical Engineering Hainan UniversityHaikou 570228China

Resolution is undoubtedly the most important parameter in optical microscopy by providing an estimation on the maximum resolving power of a certain optical microscope. For centuries, the resolution of an optical microscope is generally considered to be limited only by the numerical aperture of the optical system and the wavelength of light. However, since the invention and popularity of various advanced fluorescence microscopy techniques, especially super-resolution fluorescence microscopy, many new methods have been proposed for estimating the resolution, leading to confusions for researchers who need to quantify the resolution of their fluorescence microscopes. In this paper, we firstly summarize the early concepts and criteria for predicting the resolution limit of an ideal optical system. Then, we discuss some important influence factors that deteriorate the resolution of a certain fluorescence microscope. Finally, we provide methods and examples on how to measure the resolution of a fluorescence microscope from captured fluorescence images. This paper aims to answer as best as possible the theoretical and practical issues regarding the resolution estimation in fluorescence microscopy.

关键词： fluorescence microscopy super-resolution microscopy Abbe limit Rayleigh criterion image resolution

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Ordinal distribution regression for gait-based age estimation

引用

science China(Information sciences) 2020年第2期63卷 21-34页

作者： Haiping ZHU Yuheng ZHANG Guohao LI Junping ZHANG Hongming SHAN Shanghai Key Lab of Intelligent Information Processing School of Computer Science Fudan University Department of biomedical Engineering Rensselaer Polytechnic Institute

Computer vision researchers prefer to estimate age from face images because facial features provide useful information. However, estimating age from face images becomes challenging when people are distant from the camera or occluded. A person’s gait is a unique biometric feature that can be perceived efficiently even at a distance. Thus, gait can be used to predict age when face images are not ***, existing gait-based classification or regression methods ignore the ordinal relationship of different ages, which is an important clue for age estimation. This paper proposes an ordinal distribution regression with a global and local convolutional neural network for gait-based age estimation. Specifically, we decompose gait-based age regression into a series of binary classifications to incorporate the ordinal age ***, an ordinal distribution loss is proposed to consider the inner relationships among these classifications by penalizing the distribution discrepancy between the estimated value and the ground truth. In addition,our neural network comprises a global and three local sub-networks, and thus, is capable of learning the global structure and local details from the head, body, and feet. Experimental results indicate that the proposed approach outperforms state-of-the-art gait-based age estimation methods on the OULP-Age dataset.

关键词： computer vision deep learning ordinal distribution regression global and local features gaitbased age estimation

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

A new scheme of coded ultrasound using Golay codes

引用

Journal of Zhejiang University-science C(Computers and Electronics) 2010年第6期11卷 476-480页

作者： Cheng JIN Si-ping CHEN Zheng-di QIN Tian-fu WANG school of Medicine Shenzhen University Shenzhen 518060 China school of biomedical Engineering and Instrument science Zhejiang University Hangzhou 310027 China

Golay codes are the most practical code in coded ultrasound imaging *** the trade-off for perfect range sidelobe cancellation is the requirement for two firings, thus resulting in motion-dependent decoding *** view of this, we propose a new scheme using the simultaneous emission of code *** code pair is allocated to different elements of an aperture and transmitted *** process of separating the code pair from the echo received is based on the orthogonality of the code *** last the autocorrelation functions of the individual Golay codes are added *** simultaneous emission of code pairs instead of two firings recovers the frame rate loss, and eliminates the motion-dependent decoding *** theoretical analysis and simulations show that the scheme can be used to eliminate the tissue motion effects.

关键词： Coded ultrasound Golay codes Tissue motion Ultrasonic imaging

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Bacteroides fragilis enterotoxin upregulates heme oxygenase-1 in dendritic cells via reactive oxygen species-,mitogen-activated protein kinase-,and Nrf2-dependent pathway

引用

World Journal of Gastroenterology 2020年第3期26卷 291-306页

作者： Su Hyuk Ko Jong Ik Jeon Hyun Ae Woo Jung Mogg Kim Department of Microbiology and Department of biomedical science Hanyang University College of Medicine and Graduate School of Biomedical Science and EngineeringSeoul 04763South Korea Graduate school of Pharmaceutical sciences Ewha Womans UniversitySeoul 03760South Korea

BACKGROUND Enterotoxigenic Bacteroides fragilis(ETBF)causes colitis and diarrhea,and is considered a candidate pathogen in inflammatory bowel diseases as well as colorectal *** diseases are dependent on ETBF-secreted toxin(BFT).Dendritic cells(DCs)play an important role in directing the nature of adaptive immune responses to bacterial infection and heme oxygenase-1(HO-1)is involved in the regulation of DC *** To investigate the role of BFT in HO-1 expression in *** Murine DCs were generated from specific pathogen-free C57BL/6 and Nrf2−/−knockout *** were exposed to BFT,after which HO-1 expression and the related signaling factor activation were measured by quantitative RT-PCR,EMSA,fluorescent microscopy,immunoblot,and *** HO-1 expression was upregulated in DCs stimulated with *** BFT activated transcription factors such as NF-κB,AP-1,and Nrf2,activation of NF-κB and AP-1 was not involved in the induction of HO-1 expression in BFT-exposed ***,upregulation of HO-1 expression was dependent on Nrf2 activation in ***,HO-1 expression via Nrf2 in DCs was regulated by mitogenactivated protein kinases such as ERK and ***,BFT enhanced the production of reactive oxygen species(ROS)and inhibition of ROS production resulted in a significant decrease of phospho-ERK,phospho-p38,Nrf2,and HO-1 CONCLUSION These results suggest that signaling pathways involving ROS-mediated ERK and p38 mitogen-activated protein kinases-Nrf2 activation in DCs are required for HO-1 induction during exposure to ETBF-produced BFT.

关键词： Bacteroides fragilis enterotoxin Dendritic cells Heme oxygenase-1 Mitogen-activated protein kinases Nrf2 Signaling

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

High-resolution mapping of brain vasculature and its impairment in the hippocampus of Alzheimer's disease mice

引用

National science Review 2019年第6期6卷 1223-1238页

作者： Xiaochuan Zhang Xianzhen Yin Jingjing Zhang Anan Li Hui Gong Qingming Luo Haiyan Zhang Zhaobing Gao Hualiang Jiang school of Pharmacy East China University of Science and Technology CAS Key Laboratory of Receptor Research Shanghai Institute of Materia MedicaChinese Academy of Sciences Britton Chance Center for biomedical Photonics Wuhan National Laboratory for OptoelectronicsHuazhong University of Science and Technology MoE Key Laboratory for biomedical Photonics School of Engineering Sciences Huazhong University of Science and Technology Shanghai Institute for Advanced Immunochemical Studies and School of Life Science and TechnologyShanghai Tech University

Accumulating evidence indicates the critical importance of cerebrovascular dysfunction in the pathogenesis of Alzheimer’s disease(AD). However, systematic comparative studies on the precise brain vasculature of wild-type and AD model mice are still rare. Using an image-optimization method for analysing Micro-Optical Sectioning Tomography(MOST) data, we generated cross-scale whole-brain 3 D atlases that cover the entire vascular system from large vessels down to smallest capillaries at submicron resolution,for both wild-type mice and a transgenic(APP/PS1) mouse model of AD. In addition to distinct vascular patterns in different brain regions, we found that the main vessels of the molecular layer of the hippocampal dentate gyrus(DG-ml) undergo abrupt changes in both diameter and branch angle, spreading a unique comb-like pattern of capillaries. By using a quantitative analysis workflow, we identified in the hippocampus of AD mice an overall reduction of the mean vascular diameter, volume fraction and branch angle, with most significant impairment in the DG-ml. In addition, virtual endoscopy revealed irregular morphological features in the vessel lumen of the AD mice, potentially contributing to the impairment of blood flow. Our results demonstrate the capability of high-resolution cross-scale evaluation of brain vasculature and underscore the importance of studying hippocampal microcirculation for understanding AD pathogenesis.

关键词： Micro-Optical Sectioning Tomography image optimization Alzheimer’s disease impaired hippocampal vasculature virtual vascular endoscopy

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Studying the Effect of the Pre-Stimulation Paradigm on Steady-State Visual Evoked Potentials with Dynamic Models Based on the Zero-Pole Analytical Method

引用

Tsinghua science and Technology 2020年第3期25卷 435-446页

作者： Shangen Zhang Xu Han Xiaorong Gao the Department of biomedical Engineering School of Medicine Tsinghua University

This study explored methods for improving the performance of Steady-State Visual Evoked Potential(SSVEP)-based Brain-Computer Interfaces(BCI), and introduced a new analytical method to quantitatively analyze and reflect the characteristics of SSVEP. We focused on the effect of the pre-stimulation paradigm on the SSVEP dynamic models and the dynamic response process of SSVEP, and performed a comparative analysis of three pre-stimulus paradigms(black, gray, and white). Four dynamic models with different orders(second-and third-order)and with and without a zero point were used to fit the SSVEP envelope. The zero-pole analytical method was adopted to conduct quantitative analysis on the dynamic models, and the response characteristics of SSVEP were represented by zero-pole distribution characteristics. The results of this study indicated that the pre-stimulation paradigm affects the characteristics of SSVEP, and the dynamic models had good fitting abilities with SSVEPs under various types of pre-stimulation. Furthermore, the zero-pole characteristics of the models effectively characterize the damping coefficient, oscillation period, and other SSVEP characteristics. The comparison of zeros and poles indicated that the gray pre-stimulation condition corresponds to a lower damping coefficient, thus showing its potential to improve the performance of SSVEP-BCIs.

关键词： Steady-State Visual Evoked Potential(SSVEP) dynamic model pre-stimulation zero and pole analysis brain-computer interface

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Flexible Graphene Field‑Effect Transistors and Their Application in Flexible biomedical Sensing

引用

Nano-Micro Letters 2025年第2期17卷 252-313页

作者： Mingyuan Sun Shuai Wang Yanbo Liang Chao Wang Yunhong Zhang Hong Liu Yu Zhang Lin Han Institute of Marine science and Technology Shandong UniversityQingdao 266237ShandongPeople’s Republic of China State Key Laboratory of Crystal Materials Shandong UniversityJinan 250100ShandongPeople’s Republic of China school of Integrated Circuits Shandong UniversityJinan 250100ShandongPeople’s Republic of China Shandong Engineering Research Center of Biomarker and Artificial Intelligence Application Jinan250100ShandongPeople’s Republic of China

Flexible electronics are transforming our lives by making daily activities more *** to this innovation are field-effect transistors(FETs),valued for their efficient signal processing,nanoscale fabrication,low-power consumption,fast response times,and ***,known for its exceptional mechanical properties,high electron mobility,and biocompatibility,is an ideal material for FET channels and *** combination of graphene and FETs has given rise to flexible graphene field-effect transistors(FGFETs),driving significant advances in flexible electronics and sparked a strong interest in flexible biomedical ***,we first provide a brief overview of the basic structure,operating mechanism,and evaluation parameters of FGFETs,and delve into their material selection and patterning *** ability of FGFETs to sense strains and biomolecular charges opens up diverse application *** specifically analyze the latest strategies for integrating FGFETs into wearable and implantable flexible biomedical sensors,focusing on the key aspects of constructing high-quality flexible biomedical ***,we discuss the current challenges and prospects of FGFETs and their applications in biomedical *** review will provide valuable insights and inspiration for ongoing research to improve the quality of FGFETs and broaden their application prospects in flexible biomedical sensing.

关键词： Flexible Graphene Field-effect transistor Wearable Implantable Biosensor

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Low intensity ultrasound-induced apoptosis in human gastric carcinoma cells

引用

World Journal of Gastroenterology 2008年第31期14卷 4873-4879页

作者： Yi Feng Zhong-Min Tian Ming-Xi Wan Zhao-Bin Zheng Key Laboratory of biomedical Information Engineering of Ministry of Education Department of Biomedical Engineering School of Life Science and Technology Xi'an Jiaotong University Xi'an 710049 Shaanxi Province China

AIM： To investigate the low intensity ultrasound （US）- induced apoptosis in human gastric carcinoma cells and its potential mechanism and to suggest a new therapeutic approach to gastric carcinoma. METHODS： Human SGC-7901 gastric carcinoma cells were cultured in vitro and irradiated by low intensity US for 10 min at different intensities with different incubation times after irradiation. Morphologic changes were examined under microscope with trypan blue staining and then the percentage of early apoptotic cells was detected by flow cytometry （FCM） with double staining of fiuorescein isothiocyanate （FITC）- Annexin V/propidium iodide （PI）. Two-dimensional electrophoresis （2DE） was used to get the protein profile and some proteins differently expressed after US irradiation were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry （MALDI-TOF-MS）. Functional analysis was performed to investigate the mechanism of US-induced cell apoptosis. RESULTS： The percentage of apoptotic cells increased about 10% after US irradiation （12.0 W/cm^2, 12 h culture）, The percentage of early apoptosis and secondary necrosis in the US-irradiated cells increased with the increased US intensity. Moreover, apoptotic cells increased with the increased culture time after US irradiation and reached its maximum at about 12 *** new proteins appeared after US irradiation and were up or down regulated more than 2 times. Some heat shock proteins （HSPs） were found to be associated with the signal process simulating the apoptosis of cells. CONCLUSION： Low intensity US could induce apoptosis in human gastric carcinoma cells. US-induced apoptosis is related to US intensity/culture time. US-induced apoptosis may be caspases-dependent and endoplasmic reticulum （ER） stress-triggered apoptosis may also contribute to it. Proteomic experimental system is useful in finding the protein alteration in carcinoma cells after US irradiation, helping to develop a new cancer

关键词： SGC-7901 human gastric carcinoma cells Low intensity ultrasound Apoptosis Caspasesdependent Proteomics

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Free form deformation and symmetry constraint‐based multimodal brain image registration using generative adversarial nets

引用

CAAI Transactions on Intelligence Technology 2023年第4期8卷 1492-1506页

作者： Xingxing Zhu Mingyue Ding Xuming Zhang Department of biomedical Engineering School of Life Science and TechnologyMinistry of Education Key Laboratory of Molecular BiophysicsHuazhong University of Science and TechnologyWuhanChina

Multi‐modal brain image registration has been widely applied to functional localisation,neurosurgery and computational *** existing registration methods based on the dense deformation fields involve too many parameters,which is not conducive to the exploration of correct spatial correspondence between the float and reference ***,the unidirectional registration may involve the deformation folding,which will result in the change of topology during *** address these issues,this work has presented an unsupervised image registration method using the free form deformation(FFD)and the symmetry constraint‐based generative adversarial networks(FSGAN).The FSGAN utilises the principle component analysis network‐based structural representations of the reference and float images as the inputs and uses the generator to learn the FFD model parameters,thereby producing two deformation ***,the FSGAN uses two discriminators to decide whether the bilateral registration have been realised ***,the symmetry constraint is utilised to construct the loss function,thereby avoiding the deformation *** on BrainWeb,high grade gliomas,IXI and LPBA40 show that compared with state‐of‐the‐art methods,the FSGAN provides superior performance in terms of visual comparisons and such quantitative indexes as dice value,target registration error and computational efficiency.

关键词： Free‐form deformation Generative adversarial nets Multi‐modal brain image registration Structural representation Symmetry constraint

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：