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Sperm cryodamage occurs after rapid freezing phase:flow cytometry approach and antioxidant enzymes activity at different stages of cryopreservation

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Journal of animal Science and Biotechnology 2016年第4期7卷 495-503页

作者： L.S.Castro T.R.S.Hamilton C.M.Mendes M.Nichi V.H.Barnabe J.A.Visintin M.E.O.A.Assumpcao laboratory of spermatozoa biology department of animal reproductionschool of veterinary medicine and animal scienceuniversity of sao pauloBrazil laboratory of in vitro fertilization cloning and animal transgenesisdepartment of animal reproductionschool of veterinary medicine and animal scienceuniversity of sao paulosao pauloBrazil laboratory of andrology.department of animal reproduction school of veterinary medicine and animal scienceuniversity of sao paulosao pauloBrazil

Background： In order to improve the efficiency of bovine sperm cryopreservation process, it is important to understand how spermatozoa respond to differences in temperature as well as the ability to recover its own metabolism. The combination between flow cytometry approach and antioxidant enzymes activity allows a more sensible evaluation of sperm cell during cryopreservation. The aim of this study was to evaluate sperm attributes and antioxidant enzymes activity during different stages of cryopreservation process. Semen samples from Holstein bulls （n = 4） were separated in 3 treatments： fresh （37 ℃）; cooled （5 ℃）; and thawed. Evaluation occurred at 0 h and 2 h after incubation. Membrane integrity, mitochondrial membrane potential （MMP） and DNA damages were evaluated by flow cytometry; activities of antioxidant enzymes such as catalase, superoxide dismutase and gluthatione peroxidase were measured by spectrofotometry. Results： There was an increase in the percentage of sperm with DNA damage in the thawed group, compared to fresh and cooled, and for 2 hs of incubation when compared to 0 h. Considering MMP, there was an increase in the percentage of cells with medium potential in thawed group when compared to fresh and cooled groups. Opposingly, a decrease was observed in the thawed group considering high mitochondrial potential. Also in the thawed group, there was an increase on cells with damaged acrosome and membrane when compared to fresh and cooled groups. Significant correlations were found between antioxidant enzymes activity and membrane or mitochondrial parameters. Conclusion： Based on our results, we conclude that cryopreservation affects cellular and DNA integrity and that the critical moment is when sperm cells are exposed to freezing temperature. Also, our study indicates that intracellular antioxidant machinery （SOD and GPX enzymes） is not enough to control cryodamage.

关键词： Bovine DNA integrity JC-1 Sperm viability

Perturbations in the uterine luminal fluid composition are detrimental to pregnancy establishment in cattle

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Journal of animal Science and Biotechnology 2019年第1期10卷 56-66页

作者： Thiago Martins Guilherme Pugliesi Mariana Sponchiado Angela M.Gonella-Diaza Oscar A.Ojeda-Rojas Frederich D.Rodriguez Roney S.Ramos Andrea C.Basso Mario Binelli Department of animal Reproduction School of Veterinary Medicine and Animal ScienceUniversity of Sao Paulo Department of Nutrition and animal Production School of Veterinary Medicine and Animal ScienceUniversity of Sao Paulo University Corporation of Huila ABS Global In Vitro Brasil Department of animal Sciences University of Florida

Background: A major,unresolved issue is how the uterine microenvironment determines pregnancy success in *** implantation,conceptus development depends on the uterine secretome(i.e.,histotroph).Despite its pivotal role,little is known about the dynamics of histotroph synthesis and changes in composition throughout the early diestrus and the relevance to pregnancy *** hypothesize that disturbances on histotroph composition affect the establishment of *** was to disturb histotroph composition at early diestrus and verify the effects on:(Exp.1) timing to restore its composition; and(Exp.2) pregnancy rate after multiple-embryo *** cycle of multiparous Nelore cows were synchronized and estrus was considered d 0(D0) of the *** was through flushing each uterine horn with 30 m L of DMPBS and collecting the resulting uterine luminal flushing(ULF) on D1; D4; D7; D1 + D4 + *** group remained *** Exp.1,ULF was collected on D7.5 from all animals and used for quantification of total protein concentration and abundance of *** Exp.2,three in vitro-produced embryos were transferred to the uterine horn ipsilateral to the ovary containing the CL on D7.5 and pregnancy was checked on D25 by ***: In Exp.1,ULF collection on D4 or D7 increased(1.5-to 2.2-folds) the total protein concentration and albumin *** collection on D1 did not alter(P > 0.10) these *** Exp.2,ULF collected on D4 or D7 decreased pregnancy rates to approximately half of that measured in the remaining ***: Subtle perturbations imposed to the native intrauterine milieu,such as those caused by a single,low-volume collection of ULF,profoundly disturbs intrauterine composition and pregnancy *** least 4 d were necessary for the uterus to recover its composition and the functional capacity to carry post-implantation gestation.

关键词： Albumin Embryo Histotroph Protein

Mammary stem cells:expansion and animal productivity

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Journal of animal Science and Biotechnology 2014年第4期5卷 371-378页

作者： Ratan K Choudhary school of animal Biotechnology Guru Angad Dev Veterinary and Animal Science University

Identification and characterization of mammary stem cells and progenitor cells from dairy animals is important in the understanding of mammogenesis, tissue turnover, lactation persistency and regenerative therapy. It has been realized by many investigators that altered lactation, long dry periods （non-milking period between two consecutive lactation cycles）, abrupt cessation of lactation （common in water buffaloes） and disease conditions like mastitis, greatly reduce milk yield thus render huge financial losses within the dairy sector. Cellular manipulation of specialized cell types within the mammary gland, called mammary stem cells （MaSCs）/progenitor cells, might provide potential solutions to these problems and may improve milk production. In addition, MaSCs/progenitor cells could be used in regenerative therapy against tissue damage caused by mastitis. This review discusses methods of MaSC/progenitor cell manipulation and their mechanisms in bovine and caprine animals. Author believes that intervention of MaSCs/progenitor cells could lessen the huge financial losses to the dairy industry globally.

关键词： Hormones Mammary stem cell Manipulation Milk production Ruminant Xanthosine

The compound(E)-2-(3,4-dihydroxystyryl)-3-hydroxy-4H-pyran-4-one alleviates neuroinflammation and cognitive impairment in a mouse model of Alzheimer's disease

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Neural Regeneration Research 2025年第11期20卷 3330-3344页

作者： Xueyan Liu Wei Wu Xuejuan Li Chengyan Wang Ke Chai Fanru Yuan Huijuan Zheng Yuxing Yao Chenlu Li Zu-Cheng Ye Daijun Zha Department of Medicinal Chemistry School of PharmacyFujian Medical UniversityFuzhouFujian ProvinceChina Fujian Provincial Key Laboratory of Brain Aging and Neurodegenerative Diseases School of Basic Medical SciencesFujian Medical UniversityFuzhouFujian ProvinceChina Institute of Laboratory animal Center Fujian Medical UniversityFuzhouFujian ProvinceChina Department of Neurosurgery Neurosurgery Research InstituteThe First Affiliated HospitalFujian Medical UniversityFuzhouFujian ProvinceChina Department of Hyperbaric Oxygen National Regional Medical CenterBinhai Campus of the First Affiliated HospitalFujian Medical UniversityFuzhouFujian ProvinceChina

Previous studies have shown that the compound(E)-2-(3,4-dihydroxystyryl)-3-hydroxy-4H-pyran-4-one(D30),a pyromeconic acid derivative,possesses antioxidant and anti-inflammatory properties,inhibits amyloid-β aggregation,and alleviates scopolamine-induced cognitive impairment,similar to the phase Ⅲ clinical drug *** this study,we established a mouse model of Alzheimer's disease via intracerebroventricular injection of fibrillar amyloid-β to investigate the effect of D30 on fibrillar amyloid-β-induced *** results showed that D30 alleviated fibrillar amyloid-β-induced cognitive impairment,promoted fibrillar amyloid-β clearance from the hippocampus and cortex,suppressed oxidative stress,and inhibited activation of microglia and astrocytes.D30 also reversed the fibrillar amyloid-β-induced loss of dendritic spines and synaptic protein ***,we demonstrated that exogenous fibrillar amyloid-βintroduced by intracerebroventricular injection greatly increased galectin-3 expression levels in the brain,and this increase was blocked by *** the role of D30 in clearing amyloid-β,inhibiting neuroinflammation,protecting synapses,and improving cognition,this study highlights the potential of galectin-3 as a promising treatment target for patients with Alzheimer's disease.

关键词： Alzheimer's disease amyloid-β astrocyte cognitive impairment D30 dendritic spines galectin-3 microglia neuroinflammation neuron

Diet xylo-oligosaccharide supplementation improves growth performance, immune function, and intestinal health of broilers

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animal Nutrition 2024年第2期17卷 165-176页

作者： Zhiyong Rao Yue Li Xiaopeng Yang Yongpeng Guo Wei Zhang Zhixiang Wang school of animal Science and Technology Henan Agricultural University

The effects of xylo-oligosaccharides(XOS) on broiler growth performance, immune function, and intestinal health were investigated. A total of 540 one-d-old Arbor Acres Plus broilers were randomly divided into 5 groups with 6 replicates per group and 18 chickens per replicate. Broilers in the control(CON) group received a corn-soybean meal based basal diet, those in the antibiotics(ANT) group received the basal diet plus 500 mg/kg oxytetracycline, and those in XOS groups received the basal diet plus 150, 300, or 450 mg/kg XOS. Compared with CON, the body weight at 42 d and average daily gain from 1 to 42 d were significantly increased in the 150, 450 mg/kg XOS-added and ANT groups(P = 0.018),and the relative expression of claudin-1 and ZO-1 m RNA in the ileum was significantly higher in the 300and 450 mg/kg XOS-added groups(P < 0.001). The feed conversion ratios(P < 0.001) and abdominal fat rates(P = 0.012) of broilers from 1 to 42 d of age were significantly lower in all XOS-added groups than in the control group. Splenic index(P = 0.036) and bursa of Fabricius index(P = 0.009) were significantly better in the ANT group and each XOS-added group than in the control group. Compared to CON and ANT,serum Ig A(P = 0.007) and Ig G(P = 0.002) levels were significantly higher in the 300 mg/kg XOS-added group, and the relative abundance of short-chain fatty acid-producing genera(Alistipes) was also significantly higher(P < 0.001). Meanwhile, ileal villus height(P < 0.001) and ratio of villus height to crypt depth(V:C)(P = 0.001) were significantly increased in XOS-added broilers. In analysis of relationships between cecal microbes and the physical barrier of the gut, [Ruminococcus]＿torques＿group was positively correlated with m RNA expression of ileal ZO-1 and claudin-1(P < 0.05), and Bacteroides was positively correlated with increased ileal villus height and V:C(P < 0.05). Overall, XOS addition to broiler diets improved growth performance, promoted intestinal health by enh

关键词： Xylo-oligosaccharide Growth performance Intestinal health Immunity Broiler

Cultivating Erect Milkvetch (Astragalus adsurgens Pall.) (Leguminosae) Improved Soil Properties inLoess Hilly and Gulliesin China

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Journal of Integrative Agriculture 2013年第9期12卷 1652-1658页

作者： WANG Zhan-bin WANG Qing-yi animal Science and Technology school Henan University of Science and Technology

Erect milkvetch （Astragalus adsurgens Pall.）, a leguminous grass, is a major source of fuel and forage, and has an important role in the restoration of the degraded ecosystems in central and northeastern China. The objective of this work was to investigate how erect milkvetch planting would affect the physical and chemical properties of soil in degraded arable lands. Soil samples at the depths of 0-20 and 20-40 cm were collected from erect milkvetch planting fileds at ages of 0, 1, 2 and 3 yr. Changes in soil bulk density, soil porosity, total N and P, organic matter content, available P, hydrolysable N and available K were measured. The results showed that root biomass and above-ground plant biomass were both significantly increased with plantation age. The significant increase in root nodule biomass was not observed in the first two years. However, it was significantly increased after three years. Root growth of erect milkvetch improved soil structure, and hence, decreased soil bulk density and increased soil porosity. Furthermore, the nitrogen fixation by erect milkvetch and return of erect milkvetch plant to soil increased the soil total N, hydrolysable N and organic matter content of the soil. Low concentrations of P in the soil with erect milkvetch planting could be ascribed to high plant uptakes and possibly to high sequestrations of P in plant biomass. Concentrations of K significantly increased during the first two years of erect milkvetch planting. The high accumulation of K under erect milkvetch cultivation in the first two years could partly be attributed to low plant uptake, and partly to relatively quick recycling within plant-soil systems. Three years after erect milkvetch plantingr, K accumulation at 0-20 cm soil layer was significantly lower than that from non-vegetated field sites, which could be attributed to high plant uptake. These parameters, except for soil bulk density, were all decreased with increasing soil depth. Soil total N, organic matt

关键词： Asuagalus adsurgens Pall. soil properties plantation age loess hills and gullies

Handmade cloning:recent advances,potential and pitfalls

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Journal of animal Science and Biotechnology 2015年第4期6卷 378-387页

作者： Geetika Verma JS Arora RS Sethi CS Mukhopadhyay Ramneek Verma school of animal Biotechnology Post Graduate Institute of Veterinary Education and ResearchGuru Angad Dev Veterinary and Animal Sciences University

Handmade cloning （HMC） is the most awaited, simple and micromanipulator-ffee version of somatic cell nuclear transfer （SCNT）. The requirement of expensive micromanipulators and skilled expertise is eliminated in this technique, proving it as a major revolution in the field of embryology. During the past years, many modifications have been incorporated in this technique to boost its efficiency. This alternative approach to micromanipulator based traditional cloning O-C） works wonder in generating comparable or even higher birth rates in addition to declining costs drastically and enabling cryopreservation. This technique is not only applicable to intraspecies nuclear transfer but also to interspecies nuclear transfer （iSCNT） thus permitting conservation of endangered species. It also offers unique possibilities for automation of SCNT which aims at production of transgenic animals that can cure certain human diseases by producing therapeutics hence, providing a healthier future for the wellbeing of humans. The present review aims at highlighting certain aspects of HMC including recent advancements in procedure and factors involved in elevating its efficiency besides covering the potentials and pitfalls of this technique

关键词： Handmade cloning Micromanipulator Somatic cell nuclear transfer Transgenic Zona free cloning

Calcium potentiates the effect of estradiol on PGF2α production in the bovine endometrium

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Journal of animal Science and Biotechnology 2015年第2期6卷 215-221页

作者： Claudia Maria Bertan Membrive Pauline Martins da Cunha Flávio Vieira Meirelles Mario Binelli S?o Paulo State University Department of animal Reproduction School of Veterinary Medicine and Animal Science University of S?o Paulo Department of Veterinary Medicine School of Animal Sciences and Food Engineering University of S?o Paulo

Background： Estradiol （E2） is required for luteolysis in cows and its injection stimulates prostaglandin F2α （PGF2α） release. The main goal of our study was to investigate the ability of endometrial explants and cells treated with E2 and the calcium ionophore （CI） A23187 to synthesize PGF2α. Results： Treatment with E2 in vivo resulted in a 48.4% increase of PGF2α production by endometrial explants treated in vitro with A23187. Production of PGF20 was better stimulated with A23187 at concentrations of 10^-6 and 10^-5 mol/L compared with other concentrations used. The concentration of PGF2α for untreated bovine endometrial cell cultures was 33.1 pg/mL, while for cultures treated with E2, A23187, or a combination of E2 and A23187, the PGF2α concentration was 32.5, 92.4 and 145.6 pg/mL, respectively. Conclusions： Treatment with A23187 tended to stimulate PGF20 production. In the presence of E2, A23187 significantly stimulated PGF20 synthesis. It appears that A23187 potentiates the effects of E2 with respect to synthesis of endometrial PGF2α in cattle.

关键词： animal reproduction Cattle Estradiol Luteolysis PGF2α synthesis Reproductive physioloqy

Role of metabolic dysfunction and inflammation along the liver-brain axis in animal models with obesity-induced neurodegeneration

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Neural Regeneration Research 2025年第4期20卷 1069-1076页

作者： Evridiki Asimakidou Eka Norfaishanty Saipuljumri Chih Hung Lo Jialiu Zeng Department of Clinical Neurosciences University of CambridgeCambridgeUK school of Applied Science Republic PolytechnicSingaporeSingapore Lee Kong Chian school of Medicine Nanyang Technological UniversitySingaporeSingapore

The interaction between metabolic dysfunction and inflammation is central to the development of neurodegenerative diseases such as Alzheimer’s disease and Parkinson’s ***-related conditions like type 2 diabetes and non-alcoholic fatty liver disease exacerbate this *** lipid accumulation,particularly in the liver,initiates a cascade of inflammatory processes that extend to the brain,influencing critical metabolic regulatory *** and palmitate,key lipid components,along with lipid transporters lipocalin-2 and apolipoprotein E,contribute to neuroinflammation by disrupting blood–brain barrier integrity and promoting *** insulin resistance further exacerbates brain insulin resistance and *** interventions targeting peripheral lipid metabolism and insulin signaling pathways have shown promise in reducing neuroinflammation in animal ***,translating these findings to clinical practice requires further investigation into human *** conclusion,metabolic dysfunction,peripheral inflammation,and insulin resistance are integral to neuroinflammation and *** these complex mechanisms holds potential for identifying novel therapeutic targets and improving outcomes for neurodegenerative diseases.

关键词： Alzheimer’s disease inflammatory cytokines insulin resistance lipid