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Insect-specific RNA virus affects the stylet penetration activity of brown citrus aphid(Aphis citricidus)to facilitate its transmission

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Insect Science 2024年第1期31卷 255-270页

作者： Xin An Qiaoying Gu Jing Wang Tengyu Chang Wei Zhang Jin-Jun Wang Jinzhi Niu Key laboratory of Entomology and Pest Control Engineering College of Plant ProtectionAcademy of Agricultural ScienceSouthwest UniversityChongqingChina

Sap-sucking insects often transmit plant viruses but also carry insect viruses,which infect insects but not plants.The impact of such insect viruses on insect host biology and ecology is largely unknown.Here,we identified a novel insect-specific virus carried by brown citrus aphid(Aphis citricidus),which we tentatively named Aphis citricidus picornavirus(AcPV).Phylogenetic analysis discovered a monophyletic cluster with AcPV and other unassigned viruses,suggesting that these viruses represent a new family in order Picornavirales.Systemic infection with AcPV triggered aphid antiviral immunity mediated by RNA interference,resulting in asymptomatic tolerance.Importantly,we found that AcPV was transmitted horizontally by secretion of the salivary gland into the feeding sites of plants.AcPV influenced aphid stylet behavior during feeding and increased the time required for intercellular penetration,thus promoting its transmission among aphids with plants as an intermediate site.The gene expression results suggested that this mechanism was linked with transcription of salivary protein genes and plant defense hormone signaling.Together,our results show that the horizontal transmission of AcPV in brown citrus aphids evolved in a manner similar to that of the circulative transmission of plant viruses by insect vectors,thus providing a new ecological perspective on the activity of insect-specific viruses found in aphids and improving the understanding of insect virus ecology.

关键词： electrical penetration graph feeding behavior insect virus transmission Picornavirales salivary protein

The appearance of cytoplasmic cytochrome C precedes apoptosis during Drosophila salivary gland degradation

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Insect Science 2024年第1期31卷 157-172页

作者： Shihui Long Wenxin Cao Yongyu Qiu Ruohan Deng Jiali Liu Lidan Zhang Renke Dong Fengxin Liu Sheng Li Haigang Zhao Na Li Kang Li Guangdong Provincial Key laboratory of Insect Developmental Biology and Applied Technology Institute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhouChina Guangdong laboratory for Lingnan Modern Agriculture South China Agricultural UniversityGuangzhouChina Guangmeiyuan R&D Center Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied TechnologySouth China Normal UniversityMeizhouGuangdong ProvinceChina School of Food Science and Engineering South China University of TechnologyGuangzhouChina ChemPartner PharmaTech Co. LtdJiangmenGuangdong ProvinceChina

Apoptosis is an important process for organism development that functions to eliminate cell damage,maintain homeostasis,and remove obsolete tissues during morphogenesis.In mammals,apoptosis is accompanied by the release of cytochrome C(Cyt-c)from mitochondria to the cytoplasm.However,whether this process is conserved in the fruit fly,Drosophila melanogaster,remains controversial.In this study,we discovered that during the degradation of Drosophila salivary gland,the transcription of mitochondria apoptosis factors(MAPFs),Cyt-c,and death-associated APAF1-related killer(Dark)encoding genes are all upregulated antecedent to initiator and effector caspases encoding genes.The proteins Cyt-c and the active caspase 3 appear gradually in the cytoplasm during salivary gland degradation.Meanwhile,the Cyt-c protein colocates with mito-GFP,the marker indicating cytoplasmic mitochondria,and the change in mitochondrial membrane potential coincides with the appearance of Cyt-c in the cytoplasm.Moreover,impeding or promoting 20E-induced transcription factor E93 suppresses or enhances the staining of Cyt-c and the active caspase 3 in the cytoplasm of salivary gland,and accordingly decreases or increases the mitochondrial membrane potential,respectively.Our research provides evidence that cytoplasmic Cyt-c appears before apoptosis during Drosophila salivary gland degradation,shedding light on partial conserved mechanism in apoptosis between insects and mammals.

关键词： active caspase 3 apoptosis Cyt-c Drosophila salivary gland

Emerging roles of exosomes in oral diseases progression

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International Journal of Oral Science 2024年第1期16卷 36-51页

作者： Jiayi Wang Junjun Jing Chenchen Zhou Yi Fan State Key laboratory of Oral Diseases&National Center for Stomatology&National Clinical Research Center for Oral Diseases&Department of Cariology and Endodontics West China Hospital of StomatologySichuan UniversityChengduChina State Key laboratory of Oral Diseases&National Center for Stomatology&National Clinical Research Center for Oral Diseases&West China Hospital of Stomatology Sichuan UniversityChengduChina State Key laboratory of Oral Diseases&National Center for Stomatology&National Clinical Research Center for Oral Diseases&Department of Pediatric Dentistry West China Hospital of StomatologySichuan UniversityChengduChina

Oral diseases, such as periodontitis, salivary gland diseases, and oral cancers, significantly challenge health conditions due to their detrimental effects on patient's digestive functions, pronunciation, and esthetic demands. Delayed diagnosis and non-targeted treatment profoundly influence patients' prognosis and quality of life. The exploration of innovative approaches for early detection and precise treatment represents a promising frontier in oral medicine.

关键词： diseases diagnosis salivary

Role of aminopeptidase N-like in the acquisition of begomoviruses by Bemisia tabaci,the whitefly vector

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Insect Science 2024年第3期31卷 707-719页

作者： Yun-Yun Fan Yao Chi Na Chen Wilmer J.Cuellar Xiao-Wei Wang Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang ProvinceInstitute of Insect SciencesZhejiang UniversityHangzhouChina Zhejiang Tianmushan National Nature Reserve Administration HangzhouChina Institute of Plant Protection Liaoning Academy of Agricultural SciencesShenyangChina Virology laboratory Cassava ProgramInternational Center for Tropical Agriculture(CIAT)PalmiraColombia

Sri Lankan cassava mosaic virus(SLCMV)is a prominent causative agent of cassava mosaic disease in Asia and relies on the whitefly Bemisia tabaci cryptic complex for its transmission.However,the molecular mechanisms involved in SLCMV transmission by B.tabaci have yet to be understood.In this study,we identified an aminopeptidase N-like protein(BtAPN)in B.tabaci Asia II 1,an efficient vector of SLCMV,which is involved in the SLCMV transmission process.Through the use of glutathione S-transferase pull-down assay and LC-MS/MS analysis,we demonstrated the interaction between BtAPN and the coat protein(CP)of SLCMV.This interaction was further confirmed in vitro,and we observed an induction of BtAPN gene expression following SLCMV infection.By interfering with the function of BtAPN,the quantities of SLCMV were significantly reduced in various parts of B.tabaci Asia II 1,including the whole body,midgut,hemolymph,and primary salivary gland.Furthermore,we discovered that BtAPN is conserved in B.tabaci Middle East-Asia Minor 1(MEAM1)and interacts with the CP of tomato yellow leaf curl virus(TYLCV),a begomovirus known to cause severe damage to tomato production.Blocking BtAPN with antibody led to a significant reduction in the quantities of TYLCV in whitefly whole body and organs/tissues.These results demonstrate that BtAPN plays a generic role in interacting with the CP of begomoviruses and positively regulates their acquisition by the whitefly.

关键词： BtAPN coat protein SLCMV TYLCV virus acquisition whitefly

Evaluation and salivary biochemistry associations of sour-salty taste interaction

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Journal of Future Foods 2024年第4期4卷 376-382页

作者： Hongnan Jiang Ye Chen Jianshe Chen Louise Dye Xinmiao Wang laboratory of Food Oral Processing School of Food Science and BiotechnologyZhejiang Gongshang UniversityHangzhou 310018China Zhejiang-UK Joint laboratory of Food Sensory Science Zhejiang Gongshang UniversityHangzhou 310018China School of Food Science and Nutrition University of LeedsLeeds LS29JTUK

Taste interaction is a well-known phenomenon in sensory stodies,but how to quantify the magnitode of the effect of one tastant on other taste attribute(s)is still largely unsolved,let alone further explorations from a salivary biochemistry perspective.Upon acquiring the individual taste threshold and evaluating the just noticeable difference(JND).this stody firstly established a quantitative method to assess the magnitude of taste interaction in a binary taste mixture by evaluating the concentration difference upon psychologically-comparable stimulation.The change of salty taste intensity(CSI)was therefore defined as the subtraction of JND using sour-salty taste stimulant from JND using pure salty taste stimulant,with a dimension of concentration in mmol/L.CSI was then used to demonstrate how citric acid affected salty taste perception in a salty-sour binary taste mixture in 16 young and healthy participants.Concentrations of citric acid used in the binary taste mixtore were 0.***.0.09.0.***.0.24 and 0.40 mmolL,respectively.and results showed that salty taste perception was enhanced(average CSI of 0.93 mmolL)when the citric acid concentration was low(at 0.05 and 0.09 mmolL).but with an increasing concentration of citric acid ranging from 0.14 to 0.40 mmol/L,this effect gradually turned from enhancement to suppression of salty taste perception(correspondingly a continoously decreasing CSI all the way down to an average of-2.94 mumol/L).It was also found that cystatin SN concentration in participants'unstimulated saliva samples was significantly negatively correlated with salty taste threshold(with and without the presence of citric acid),and it was one of the most significant factors affecting CSI,s shown in multiple regression analysis.Carbonic anhydrase VI concentration in participants'unstimulated saliva sanples was also found to be significantly negatively correlated with salty taste recognition(with and without presence of citric acid).but it did not pose significant effect on CSI.From these results,this study

关键词： Taste interaction evaluation Sensory analysis Salty taste perception Salivay biochemical components

Pleomorphic adenoma(mixed tumor)of the upper lip:A case report

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World Journal of Clinical Cases 2024年第17期12卷 3138-3143页

作者： Midion Mapfumo Chidzonga Leonard Mahomva Blessing Zambuko Welcome Muungani Department of Oral HealthUniversity of Zimbabwe Faculty of Medicine and Health Sciences and Lancet LaboratoryHarareZimbabwe

BACKGROUND salivary gland tumors are relatively rare.Most minor salivary gland tumors are malignant with benign tumors accounting for 18%of the tumors.Pleomorphic adenoma(PA)is the most common salivary gland tumor.Lip PA is uncommon with 9.8%occurring in the upper lip.We are adding on the knowledge of the rare upper lip PA(benign mixed tumor).CASE SUMMARY We report an upper lip PA(benign mixed tumor)in a 28-year-old man.His complaint was a painless swelling on the upper lip.A painless,non-tender,well-circumscribed,slightly mobile,sessile,nodular,and rubbery(in consistency)tumor measuring 5.0 cm x 2.0 cm was noted on the left side of his upper lip.The overlying skin was not fixed and of normal color.There was no ulceration,and palpation did not elicit pain or bleeding.There was no history of trauma.Blunt dissection was used to completely excise the nodular,whitish,and encapsulated tumor.Microscopy showed a well-circumscribed and partly encapsulated biphasic lesion,with large lobules of myxo-chondroid stroma and intervening cellular nodules of basaloid cells,well-formed tubules containing eosinic secretion,and nests of myoepithelial cells.A diagnosis of PA(benign mixed tumor)was confirmed.CONCLUSION Blunt dissection is indicated to preserve the cosmesis and function of the upper lip.

关键词： Pleomorphic adenoma Upper lip Minor salivary gland tumor Benign mixed tumor Case report

Nasal vaccination of triple-RBD scaffold protein with flagellin elicits long-term protection against SARS-CoV-2 variants including JN.1

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Signal Transduction and Targeted Therapy 2024年第5期9卷 2312-2323页

作者： Xian Li Mengxin Xu Jingyi Yang Li Zhou Lin Liu Min Li Shasha Wang Mei-Qin Liu Zhixiang Huang Zhen Zhang Shuning Liu Yunqi Hu Haofeng Lin Bowen Liu Ying Sun Qingguo Wu Zheng-Li Shi Ke Lan Yu Chen Huimin Yan Yao-Qing Chen Wuhan Institute of Virology Chinese Academy of SciencesWuhanChina Vaccine and Immunology Research Center Translational Medical Research InstituteShanghai Public Health Clinical CenterFudan UniversityShanghaiChina University of Chinese Academy of Sciences BeijingChina School of Public Health(Shenzhen) Shenzhen Campus of Sun Yat-sen UniversityShenzhenGuangdongChina State Key laboratory of Virology Modern Virology Research CenterCollege of Life SciencesWuhan UniversityWuhanChina Aerosol Bio-Tech(Suzhou)Co. LTDSuzhouJiangsuChina National Medical Products Administration Key laboratory for Quality Monitoring and Evaluation of Vaccines and Biological Products Sun Yat-sen UniversityGuanzhouChina

Developing a mucosal vaccine against SARS-CoV-2 is critical for combatting the epidemic.Here,we investigated long-term immune responses and protection against SARS-CoV-2 for the intranasal vaccination of a triple receptor-binding domain(RBD)scaffold protein(3R-NC)adjuvanted with a flagellin protein(KFD)(3R-NC+KFDi.n).In mice,the vaccination elicited RBD-specific broad-neutralizing antibody responses in both serum and mucosal sites sustained at high level over a year.This long-lasting humoral immunity was correlated with the presence of long-lived RBD-specific IgG-and IgA-producing plasma cells,alongside the Th17 and Tfh17-biased T-cell responses driven by the KFD adjuvant.Based upon these preclinical findings,an open labeled clinical trial was conducted in individuals who had been primed with the inactivated SARS-CoV-2(IAV)vaccine.With a favorable safety profile,the 3R-NC+KFDi.n boost elicited enduring broad-neutralizing IgG in plasma and IgA in salivary secretions.To meet the challenge of frequently emerged variants,we further designed an updated triple-RBD scaffold protein with mutated RBD combinations,which can induce adaptable antibody responses to neutralize the newly emerging variants,including JN.1.Our findings highlight the potential of the KFD-adjuvanted triple-RBD scaffold protein is a promising prototype for the development of a mucosal vaccine against SARS-CoV-2 infection.

关键词： vaccination vaccine sustained

A tribo-chemical view on astringency of plant-based food substances

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Friction 2024年第7期12卷 1392-1407页

作者： Samuel S.GAMANIEL Paloma S.DUEÑAS ROBLES Hans TROMP Els H.A.de HOOG Sissi de BEER Emile van der HEIDE laboratory for Surface Technology and Tribology Faculty of Engineering TechnologyUniversity of TwenteEnschede 7522 LWthe Netherlands NIZO Food Research B.V. Ede6710 BAthe Netherlands Department of Molecules and Materials MESA+Institute for Nanotechnology University of TwenteEnschede 7500 AEthe Netherlands

Consumption of plant-based food products having high composition of polyphenols leads to the sensation of astringency.For sliding oral surfaces,friction is an essential property during the oral perception of roughness and dryness which are attributes associated with astringency.Different factors including the chemical composition of interacting layers,structure and operation of interfaces have an effect on the astringency development process.The manner of interactions occurring at oral interfaces suggest there is a system dependence of astringency and highlights the importance of adopting a tribosystems approach.Available measurement techniques have shown an existing relationship between salivary protein-polyphenol interaction and an astringent mouthfeel.Nevertheless,the tribo-chemistry involved in this multifaceted sensation remains largely unexplored in a comprehensive manner.In this review the underlying tribo-chemical processes useful in understanding the mechanism of astringency are highlighted and discussed considering current techniques employed to investigate astringency perception.Loss of lubrication on oral surfaces owing to the tribo-chemical interactions involving saliva and astringent plant proteins requires subsequent deformations of oral tissues which are significant enough to induce strains at mechanoreceptor locations,leading to the sensation of astringency.It is proposed that micro-scale contact modelling on the interaction of food particles/aggregates,boundary layers and oral surfaces shows potential in addressing the knowledge gap between tribo-chemical measurement techniques and panel tests,making it possible to attain a predictor for astringency.

关键词： oral lubrication astringency tribo-chemistry tribology

Research progress toward the influence of mosquito salivary proteins on the transmission of mosquito-borne viruses

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Insect Science 2024年第3期31卷 663-673页

作者： Zhao-Yang Wang Kai-Xiao Nie Ji-Chen Niu Gong Cheng Tsinghua University-Peking University Joint Center for Life Sciences School of MedicineTsinghua UniversityBejingChina Institute of Infectious Diseases Shenzhen Bay LaboratoryShenzhenGuangdongChina Department of Pathogen Biology School of Basic MedicineShandong First Medical University&Shandong Academy of Medical SciencesJinanChina

Mosquito-borne viruses(MBVs)are a large class of viruses transmitted mainly through mosquito bites,including dengue virus,Zika virus,Japanese encephalitis virus,West Nile virus,and chikungunya virus,which pose a major threat to the health of people around the world.With global warming and extended human activities,the incidence of many MBVs has increased significantly.Mosquito saliva contains a variety of bioactive protein components.These not only enable blood feeding but also play a crucial role in regulating local infection at the bite site and the remote dissemination of MBVs as well as in remodeling the innate and adaptive immune responses of host vertebrates.Here,we review the physiological functions of mosquito salivary proteins(MSPs)in detail,the influence and the underlying mechanism of MSPs on the transmission of MBVs,and the current progress and issues that urgently need to be addressed in the research and development of MSP-based MBV transmission blocking vaccines.

关键词： immune response mosquito-borne virus mosquito salivary protein saliva vaccine transmission