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THE　SIGNIFICANCE　OF　P53　GENE　MUTATIONS　AND　EXPRESSIONS　IN　HUMAN　COLORECTAL　TUMORS

Chinese Journal of Cancer Research 1996年第2期8卷 109-112页

作者：钱桦郁宝铭周锡庚王瑞年黄薇陈赛娟陈竺 Department of Surgery Xinhua Hospital Shanghai Second Medical University Shanghai Department of Surgery Ruijin Hospital SSMU Shanghai Department of Pathology SSMU Shanghai Department of Hematology Ruijin Hospital SSMU Shanghai

Using a polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) approach we analyzed 18 human colorectal adenocarcinomas for mutations in exons 5,6,7,8 of p53 gene. At the same time,p53 gene product expression was studied immunohistochemically in these 18 case in frozen sections. The expression of p53 protein was also immunohistochemically studied in formalin-fixed paraffin embeded spccimens of 76 colorectal adenocarcinomas and 112colorectal polyps. Eigbt out of 18 cases (44%) showed a variant band indicative of a mutation in exons 5-6 of p53gene 7 out of 8 cascs (88%) with p53 gene mutations were positivelystanined for P53. There was no significant correlation between p53. expression and clinicopathological manifestations and prognosis. but the strongest staining was cncountered in those cases with well differentiated and early stage adenocarcinomas,while weaker staining was encountered in Poorly differentiated and mucoid adenotarcinomas. p53expression was not observed in proliferative polyps and adenomas with low grade dysplasia. The frequency of p53expression reached 88% (p<0.001) when adenoma showed malignant change. Aiuong three types of adenomas, p53 expression was most frequent in villous type (P<0.05). The frequencies of p53 expression in adenoma, adenoma with malignant change and adenocarcinoma were 4%, 88% and 51% respectively.These indicate that genetic changes of p53 gene play an important role in the transformation from benign adeuoma to adenocarcinoma. p53 immunohistochcmistry can be used as a surrogate marker for p53 gene mutation for early discovery of colorectal adenocarcinomas.

关键词： Colorectal neoplasms p53 gene Mutation,Expression PCR-SSCP

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SCANNING ELECTRON MICROSCOPIC STUDY OF FETAL CHICKEN CALVARIAL OSTEOBLAST-LIKE CELLS CULTURED IN VITRO

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Medical Bulletin of Shanghai Jiaotong University 1993年第2期16卷 54-59页

作者：柴本甫汤雪明徐荣辉朱雅萍 Shanghai Institute of Traumatology and Orthopaedics SSMU Laboratory of Cell Biology SSMU

Three types of osteoblast-like cells with different cnfigurations could be ob-tained through culturing fetal chicken calvaria in vitro. They were spindle-shaped cells,globular cells, and polygonal or squamous cells. With passage of culture time, there werechanges in configuration so that the spindle-shaped cells and the globular cells turnedgradually into squamous cells, in quantity which increased greatly to produce confluenceand multi-layer formation of cells, and in function as evidenced by emergence ofintracytoplasmic granules, reflecting collagen synthesis.

关键词： osteoblast-like cells fetal chicken calvaria in vitro scanning electron microscope

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PRODUCTION AND TRANSFORMATION OF IVORY BONE IN OSTEOARTHRITIC FEMORAL HEAD

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Medical Bulletin of Shanghai Jiaotong University 1993年第2期16卷 60-65页

作者：柴本甫汤雪明李慧 Shanghai Institute of Traumatology and Orthopaedics SSMU Laboratory of Cell Biology SSMU

Twelve femoral heads were collected during total hip replacement forosteoarthritis of hip joint. The areas on the femoral head denuded of articular cartilageand the ivory bone were studied with scanning electron microscope. The following findingswere observed: 1. Over the area denuded of articular cartilage, the subchnodral bone platerevealed flake-like trabeculae whick were unequal in size and haphazard in array. Therewas a transformation of the flake-like trabeculae into lamellar bone tissues. 2. Over theivory bone, the lamellar bone tissues turned into Haversian systems and interstitiallamellae, thereby revealing characteristics of compact bone and became extremely hard. 3.Over the surface of the ivory bone could be seen fibrocartilaginous islands, representingreparative response from the marrow tissues.

关键词： osteoarthritis ivory bone scanning electron microscope

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NUTRITIONAL THERAPY AND LIVER CIRRHOSIS

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Medical Bulletin of Shanghai Jiaotong University 1993年第1期16卷 1-6页

作者：唐振铎沈洪亮夏宗勤戴腾昌 Service of Gastroenterology Ruijin HospitalSSMU Department of Nuclear Medicine SSMU

Among 18 patients diagnosed as posthepatitic cirrhosis of the liver at compen-sated stage, 12 were treated for 2 months with a vegetarian diet which provided a dailyintake of 2263 Kcal and 95g protein with 68g of it vegetable in origin, while the re-maining 6 patients were given a routine hospital diet and served as controls. After 2months of dietary treatment, there was a marked increase in the value of serum albuminand transferrin, an increase in 24 h urinary creatinine output and creatinine height indexwith a decrease in 24 h urinary output of urea nitrogen. The reverted A/G ratio was cor-rected and the negative nitrogen balance became positive. The protein synthetic rate turnedto become greater than the catabolic rate and the body protein flux decreased as mea-sured by the 15N-glycine tracer kinetic study. The paper concludes that a high caloric vege-tarian diet can influence favorably the whole body protein turnover in cirrhotic patientsand should thus be used at least as an adjuvant therapy in the management of these patients.

关键词： vegetarian diet liver cirrhosis

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PANCREATO-BILIARY TUMOR: MR DIAGNOSIS AND RESECTABILITY ASSESSMENT

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Journal of Shanghai Second Medical University(Foreign Language Edition) 2001年第2期13卷 113-118页

作者：陈克敏丁小龙许建荣姚秋英钟喨李磊 Department of Radiology Renji Hospital SSMU Department of Radiology Ruijin Hospital SSMU

Objective: TO evaluate the clinical value of MR multi-imaging technique in diagnosing and assessing the resectability of pancreato-biliary tumor. Methods The prospective diagnosis and resectability of 17 patients with suspicious pancreato-biliary tumors were evaluated. Surgical findings and pathologic results confirmed pancreatic adenocarcinoma in 11 cases, cholangiocarcinoma in 4, and non-neoplastic lesion in 2. MR multi-imaging protocol, including MR cross-sectional imaging, us cholangiopancreatography (MRCP ), and three-dimensional dynamic contrast-enhanced MR portography (3D DCE MRP), were performed in all patients. Results MR multi-imaging technique allowed-correct diagnosing 15 of 17 (88. 2% ) patients with pancreato-biliary tumors. The accuracy in detecting the range of tumor invasion was 64. 4%. The sensitivity, speificity, accuracy, positive, and negative predictive value of MR multi-imaging technique in assessing the resectability of pancreato-biliary tumors were 83. 3%, 77. 8%, 80. 0%, 71. 4%, and 87. 5%, respectively. Conclusion MR multi-imaging technique can not only improve the diagnostic ability of pancreato-biliary tumor, but also assess the surgical reartability of the tumor. With the fast development of MR techniques, the diagnosing and pre-operative assessment of aoncreato-biliary tumor may be more simplified and efficient by using the non-invasive "all-in-one" method.

关键词： pancreato-biliary tumor magnetic resonance imaging evaluation study

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BLOOD GAS ANALYSIS IN EXPERIMENTAL SCOLIOSIS

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Medical Bulletin of Shanghai Jiaotong University 1990年第2期13卷 13-18页

作者：柴本甫杨家裕 Shanghai Institute of Traumatology Orthopaedics SSMU Shanghai Pulmonary Department RuiJin Hospital SSMU Shanghai

Thoracic scoliosis was produced in 24 New Zealand rabbits by 2 methods of rib resection. Scoliosis was detected in X-ray film taken one month and 3 or 5 months after operation. Aortic blood specimens were obtained for gas analysis in 18 of the 24 animals. Out of the 18 animals, 5 had scoliosis with a curvature less than 15°, another 5 with a curvature between 18°-27°, while the remaining 8 with a curvature greater than 30°. The blood gas of the scoliotic rabbits was compared with that of 6 normal adult rabbits. With increase in scoliotic curvature the PaCO2 increased, while the PaO2 decreased. The PaCO2 and PaO2 of the group with curvature greater than 30°showed sigrificant difference as compared with those of the normal animals （P<0.01 and P<0.001 respectively ）, signifying a hypoxemia with severe scoliosis.Scoliotic curvature of patients greater than 30°constitutes an indication for surgical correction. As these scoliotic patients may have subclinical abnormal pulmonary function with potential danger of post-operative respiratory complication, orthopaedic surgeons should give them careful consideration before resorting to operation.

关键词： gas analysis scoliosis

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INFUSION OF ESSENTIAL AMINO ACIDS FORTIFIED WITH BRANCHED-CHAIN AMINO ACIDS IN NUTRITIONAL TREATMENT OF CHRONIC RENAL FAILURE

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Medical Bulletin of Shanghai Jiaotong University 1987年第1期 85-90页

作者：左静南朱汉威候积寿吴靖川吴继琮 Shanghai Xinhua Hospital ssmu

Intravenous supplement of essential amino acids (EAA) 12.6 g/d, fortified with branched-chain amino acids, was given to Group A, 5 patients with chronic renal failure (CRF) (mean creatinine clearance rate 6.55±2.49 ml/min) for 14 days on the basis of low protein diet (0.6 g/kg/d) , and Group B, 4 patients with CRF (mean creatinine clearance rate 9.75±3.93 ml/min) for JO days on the basis of low protein diet(0.4g/kg/d). The nitrogen balance, whole body nitrogen flux and rates of protein synthesis and catabolism and amino nitrogen utilization were markedly improved as compared with the pre-treatment levels. With treatment, the levels of some EAA in plasma such as valine, isoleucine, phenylalanine, lysine and methionine were increased, while that of tyrosine was not changed. In 4 of the Group A patients, blood urea nitrogen, creatinine and phosphate were slightly decreased after treatment, while serum calcium level was significontly increased. In 8 patients uremic symptoms were markedly ameliorated and their nutritional status apparently improved.

关键词： chronic renal failure essential amino acid branched-chain amino acid nitrogen balance

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FLOW CYTOMETRIC ANALYSIS OF DNA CONTENT FOR EARLY DETECTION, ESTIMATING PROGNOSIS IN BLADDER CANCER

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Medical Bulletin of Shanghai Jiaotong University 1989年第Z1期12卷 66-71页

作者：凌春华叶敏蒋鹤鸣安世源刘炳荣 Department of Urology Xinhua Hospital SSMU Shanghai Department of Biophysics SSMU Shanghai

Flow cytometry (FCM) was used to analyze the DNA content of epithelial cells in 26 cases of bladder cancer and 10 cases of nonneoplastic disease. Bladder tumor cells can be identified by aneuploidy or hyperdiploidy in histogram and/or a heterogeneity index (HI) greater than 2.30. The percentage of positive FCM in patients with bladder cancer was 84.62%. With respect to histological grading, it was 77.78% in grade 1, 90.91% in grade 2 and 100% in grade 3. While in nonneoplastic cases it was 10% Urinary exfoliative cytology was examined in all 26 patients with bladder cancer, with an accuracy of 61.54%. The DNA content of tumor cells increased with the increase of tumor grade and stage. Follow up showed that of 22 bladder cancer patients treated conservatively by transurethral resection or partial cystectomy, 8 recurred. The HI value in recurrent cases was higher than that in nonrecurrent cases. Among these 8 patients, 6 were correctly predicted by FCM. FCM appears to be an objective, sensitive and quantitative method for the diagnosis and monitoring of bladder cancer, and may be useful in estimating the prognosis of superficial bladder cancer.

关键词： flow cytometry bladder cancer DNA

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EXPERIMENTAL AND CLINICAL STUDY ON BILIRUBIN NEUROTOXICITY DETECTED BY VISUAL EVOKED POTENTIALS TO FLASH

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Journal of Shanghai Second Medical University(Foreign Language Edition) 2001年第1期13卷 24-27页

作者：贲晓明秦玉明吴圣楣张惠民陈舜年夏振炜 Shanghai Institute for Pediatric Research Xinhua Hospital SSMU Department of Biochemistry SSMU Department of Pediatrics Ruijin Hospital SSMU

Objective Evaluate the sensitivity and reliability of visual evoked potential to flash (F-VEP) in detecting bilirubin neurotoxicity and approach the risk parameters of bilirubin neurotoxicity in hyper-bilirubinemia newborns. Methods Based on the successful establishment of animal models for acute bilirubin encephalopathy by intraperitoneal infusion of bilirubin with a dosage of 100～200μg/g body weight to 1-week-old guinea pigs, the F-VEP was recorded in animal models and human neonates with hyperbilirubinemia, and the sensitivity and reliability of F-VEP in detecting bilirubin neurotoxicity were evaluated. Results F-VEP features and its P1 latency significantly correlated to brain adenosine triphosphate (ATP) level, neurobeha-vioral and neuropathological changes in experimental bilirubin encephalopathy; neonates with hyperbiliru-binemia showed significant F-VEP changes characterized by absence of P1 or P1 latency prolonged in 1～7-day-old newborns, especially when the jaundice was caused by immun oincompatibility and infectious diseases. Conclusion F-VEP would be a good discriminator for bilirubin neurotoxicity, and can become a promising technique in monitoring bilirubin encephalopathy.

关键词： visual evoked potentials to flash (F-VEP) guinea pig neonates hyperbilirubinemia

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STUDIES OF EFFECTS OF PORTASYSTEMIC SHUNT ON THE FUNCTION OF HEPATIC AND PANCREATIC ISLET CELLS

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Medical Bulletin of Shanghai Jiaotong University 1991年第1期14卷 6-13页

作者：邝耀麟陈治平吴志勇黄安袁济民陈聿修曾民德萧树东 Department of Surgery Renji HospitalSSMUShanghai Shanghai Institute of Digestive Disease SSMUShanghai

The present study was aimed at dynamic observation of the ef fects of end to side portacaval shunt (PCS) and end to side mesocaval shunt (MCS) in dogs on the functions of the liver and pancreatic islet cells. According to correlation between the changes of plasma insulin level in the portal vein and hepatic flow and liver morphology after PCS and MCS, we conclude that the depletion of hepatic flow is the major factor in the deterioration of liver functions. The levels of insulin and glucagon in both the peripheral vein and the portal vein were decreased after PCS and MCS. There was also depletion of pancreatic islet A and B cells and vacuolar degeneration of the pancreas. These changes were more signifcant in PCS than in MCS, suggesting that portasystemic shunt, especially total portasystemie shunt, might damage pancreatic endocrine functions.

关键词： end to side portacaval shunt end to side mesocaval shunt hepatic flow insulin glucagon pancreatic islet Cells

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