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P Fractions in Drainage Waters from the Broadbalk Continuous Wheat Experiment at Rothamsted

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Pedosphere 2004年第2期14卷 235-240页

作者： LUJia-Long S.FORTUNE P.BROOKES StateKeyLaboratoryofSoilErosionandDrylandFar~rningonLoessPlateau.NorthwestSci-TechUniversityofAgricul-tureandForestry YanglingShaanxi712100（China）.E-mail：jialongluw@*** rothamstedresearch HarpendenHeftsAL52JQ（UK）

Total P (TP), total participate P (PP), total dissolved P (TDP), molybdatereactive P (MRP) and dissolved organic P (DOP) were determined in waters from pipe-drains (at 65-cmdepth) from the Broadbalk Experiment at Rothamsted Research, UK. Average TPand PP exceeded 1 mgL^(-1) in about half of the 12 plots receiving superphosphate for the 5 measurements taken betweenDecember 2000 and April 2001. Ranging between 33.8% and 87.3% of TP, PP was the largest P fractionin drainage waters, with DOP, ranging from 0.5% to 26.2% of TP, being the smallest fraction Meanproportions of PP, MRP and DOP in TP in drainage waters were 63.4%, 32.5% and 4.1%, *** findings support previous findings that P losses from soil to drainage waters were muchlargerthan previously thought, and could therefore make a significant contribution to eutrophication.

关键词： Broadbalk experiment drainage waters phosphorus leaching

The Measurement of Membrane Potential and NO3 Activity in Root Cells Using Ion-Selective Microelectrodes

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Agricultural Sciences in China 2003年第10期2卷 1097-1101页

作者： FANXiao-rong AnthonyJMiller SHENQi-rong NanjingAgriculturalUniversity Nanjing210095P.R.China AgricultureandEnvironmentDivision RothamstedResearchHarpendenAL52JQUK）

Remobilisation of nitrate in plants, especially in vacuole of plant, is mostly related to the qua- lity of agricultural products and the high nitrogen use efficiency in plants. Ion-selective microelectrodes offer a non-destructive and non-interruptive method to measure NO 3 gradients and electric potential differences across both the plasma membrane and tonoplast. Thus, a double-barrelled microelectrode backfilled with a membrane sensor for NO 3 embedded in poly vinyl chloride (PVC) can record the NO 3 activity in cytoplasm and vacuole of a cell. This paper presented how to make this kind of microelectrode and how to do the intracellular measurements on intact plants. Our result showed that nitrate activity was about 2.7 mmol L 1 in cytoplasm while 70 mmol L 1 in vacuole, which implicated that vacuole was a pool of nitrate in plants.

关键词： Ion-selective microelectrodes Membrane potential NO 3 activity

疟蚊Anopheles gambiae和***嗅觉结合蛋白基因的鉴定和表达谱分型

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中国科学（C辑） 2004年第3期34卷 243-251页

作者：李正西 Jing-JiangZhou 沈佐锐 L.Field 中国农业大学昆虫学系北京100094 BiologicalChemistryDivision RothamstedResearchHarpendenHerts.AL52JQUK

嗅觉在疟蚊寄主发现行为过程中起主要作用.基于生物信息学技术,对按蚊嗅觉结合蛋白基因进行了全基因组分析,结果总共鉴定了32条嗅觉结合蛋白(OBP)候选基因序列.进一步采用半定量反转录聚合酶链反应(semi-quantitativeRT-PCR),以疟蚊激... 详细信息

嗅觉在疟蚊寄主发现行为过程中起主要作用.基于生物信息学技术,对按蚊嗅觉结合蛋白基因进行了全基因组分析,结果总共鉴定了32条嗅觉结合蛋白(OBP)候选基因序列.进一步采用半定量反转录聚合酶链反应(semi-quantitativeRT-PCR),以疟蚊激动蛋白基因为内部表达参照标准,研究了冈比亚按蚊所有嗅觉结合蛋白候选基因的组织特异性表达谱.结果显示:其中20个OBP候选基因在嗅觉组织(雌蚊触角)中有强的表达,这说明OBP在疟蚊嗅觉行为中起重要作用.还研究了冈比亚按蚊复合体(***)中两种最重要的疟蚊,即冈比亚按蚊(***)和阿拉伯按蚊(***)的蚊种嗅觉特异性表达型,结果发现其中12个OBP候选基因显示种间差异表达,同时发现阿拉伯按蚊触角中OBP的累积相对表达强度高于冈比亚按蚊,其比例为1441.45︰1314.12.这可能是由二者的不同寄主偏好行为所致,因为冈比亚按蚊高度嗜吸人血,而阿拉伯按蚊则介于嗜吸人血和嗜畜血之间,因此后者需要表达更多的OBPs以支持其寄主选择行为模式.疟蚊OBP基因的鉴定及其组织和蚊种特异性表达型的确定,是分析疟蚊嗅觉探测的分子机制的第一步,如嗅觉结合蛋白重组表达及其配体鉴定.

关键词：疟蚊嗅觉结合蛋白冈比亚按蚊阿拉伯按蚊半定量RT-PCR 基因鉴定基因表达生物信息学疟疾

Genetic Diversity at the Glu-1 Loci Among Hubei Bred Wheat Cultivars

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Acta Genetica Sinica 2004年第9期31卷 1014-1014页

作者： LIUYong HEGuang-Yuan ZHANGJin-Rui LINTao CHANGQi-Chang P.R.Shewry China-UKHUST-RResCropGeneticEngineeringandGenomicsJointLaboratory HuazhongUniversityofScienceandTechnologyWuhan430074China InstituteofCropBreedingandCultivation HubeiAcademyofAgriculturalSciencesWuhan430064China rothamstedresearch DepartmentofAgriculturalSciencesUniversityofBristolLongAshtonBristolBS419AFUK