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Molecular cloning and functional identification of sterol C24-methyltransferase gene from Tripterygium wilfordii

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Acta Pharmaceutica Sinica B 2017年第5期7卷 603-609页

作者： Hongyu Guan Yujun Zhao Ping Su Yuru Tong Yujia Liu Tianyuan Hu Yifeng Zhang Xianan Zhang Jia Li Xiaoyi Wu Luqi Huang Wei Gao School of Traditional Chinese Medicine Capital Medical University State Key Laboratory Breeding Base of Dao-di Herbs National Resource Center for Chinese Materia Medica China Academy of Chinese Medical Sciences Beijing University of Chinese Medicine Third Affiliated Hospital

Sterol C24-methyltransferase(SMT) plays multiple important roles in plant growth and development. SMT1, which belongs to the family of transferases and transforms cycloartenol into 24-methylene cycloartenol, is involved in the biosynthesis of 24-methyl sterols. Here, we report the cloning and characterization of a cDNA encoding a sterol C24-methyltransferase from Tripterygium wilfordii(Tw SMT1). Tw SMT1(Gen bank access number KU885950) is a 1530 bp cDNA with a 1041 bp open reading frame predicted to encode a 346-amino acid, 38.62 k Da protein. The polypeptide encoded by the SMT1 cDNA was expressed and purified as a recombinant protein from Escherichia coli(E. coli) and showed SMT activity. The expression of Tw SMT1 was highly up-regulated in T. wilfordii cell suspension cultures treated with methyl jasmonate(Me JA). Tissue expression pattern analysis showed higher expression in the phellem layer compared to the other four organs(leaf, stem, xylem and phloem), which is about ten times that of the lowest expression in leaf. The results are meaningful for the study of sterolbiosynthesis of T. wilfordii and will further lay the foundations for the research in regulating both the content of other main compounds and growth and development of T. wilfordii.

关键词： Cloning Cycloartenol C24-methyl transferase Enzymatic assay Inducible expression Tissue expression

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Regulating the microenvironment of catalytic sites by atomically precise and charge tunable Au25 nanoclusters for efficient5-hydroxymethylfurfural electro-oxidation

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Science China Chemistry 2024年

作者： Zhijuan Liu Sha-Sha Cui Lu-Yu Liu Fen Wang Guangjin Wang Zhijie Kong Xiao-Fei Liu Xin Zhang Haisong Feng Ren-Wu Huang Shuangyin Wang Shuang-Quan Zang Henan Key Laboratory of Crystalline Molecular Functional Materials Green Catalysis Center and College of ChemistryZhengzhou University School of Materials Science and Energy Engineering Foshan University State Key Laboratory of Chemical resource Engineering Beijing Advanced Innovation Center for Soft Matter Science and EngineeringBeijing University of Chemical Technology State Key Laboratory of Chem/bio-sensing and Chemometrics College of Chemistry and Chemical Engineering Hunan University

Electrochemical biomass oxidation has garnered considerable attention due to its low energy consumption and the production of high-value *** metal nanoparticle catalysts have been widely explored in electrochemical 2,5-hydroxymethylfurfural oxidation reaction （HMFOR） but limited by their inhomogeneity to build a clear structure-ability relationship at atomic *** precise metal nanoclusters with well-defined composition,high purity and unique physichemical property are one kind of ideal model *** this work,we regulate the microenvironment of catalytic sites in NiSe2utilizing atomically precise and charge tunnable *** electrochemical results reveal that after the modification of Au25nanoclusters,the HMFOR ability of NiSe2could be *** them,the NiSe2modified with Au-25clusters displayed the best electrocatalytic ability,in which the electrons were preferred to transfer from Au-25to NiSe2substrate resulting in the electrons accumulation at the electrocatalyst *** enhanced electrochemical performance of electrocatalyst can be attributed to two key factors:（1） enhanced adsorption ability towards HMF due to the strong interaction between electron enriched surface and electrophilic furan rings;（2） fast electron transfer at Au-25/NiSe2interface,resulting in the rapid structure evolution to form active NiOOH *** study serves as a valuable reference for designing highly efficient,atomically precise metal nanocluster electrocatalysts for biomass upgrading.

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Halomonas-PHB protects gnotobiotic Artemia against Vibrio and m odifies Artemia gut microbiota in xenic culture conditions

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Journal of Oceanology and Limnology 2023年第4期41卷 1292-1299页

作者： Liying SUI Xiaocui LIU Namin PAN Xue LIU Meirong GAO Key Laboratory of Marine resource Chemistry and Food Technology(TUST) Ministry of EducationTianjin University of Science and TechnologyTianjin 300457China Asian Regional Artemia Reference center College of Marine and Environmental SciencesTianjin University of Science and TechnologyTianjin 300457China

The prokaryotic cell storage compound ploy-β-hydroxybutyrate(PHB)has been considered as prebiotics that can be applied in *** this paper,the dietary effect of a PHB-accumulating Halomonas strain(HM·PHB)identified from our previous work were studied in Artemia under gnotobiotic and xenic culture conditions,in comparison of Halomonas without PHB accumulation(HM)and microalgae Isochrysis(ISO)*** gnotobiotic condition,both HM·PHB and HM served as sole food supporting Artemia *** both HM·PHB and HM feeding had no significant difference on Artemia survival percentage(P>0.05),HM·PHB significantly improved their resistance against Vibrio anguillarum challenge(Pbio-secured live feed Artemia.

关键词： Halomonas-ploy-β-hydroxybutyrate(Halomonas-PHB) Artemia survival pathogen resistance gut microbiota

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Exploring the primate genome:Unraveling the mysteries of evolution and human disease

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The Innovation 2023年第4期4卷 21-22页

作者： Yaoxi He Yafei Mao State Key Laboratory of Genetic resources and Evolution Kunming Institute of ZoologyChinese Academy of SciencesKunming 650223China National resource center for Non-Human Primates Kunming Primate Research Centerand National Research Facility for Phenotypic&Genetic Analysis of Model Animals(Primate Facility)Kunming Institute of ZoologyChinese Academy of SciencesKunming 650107China bio-X Institutes Key Laboratory for the Genetics of Developmental and Neuropsychiatric DisordersMinistry of EducationShanghai Jiao Tong UniversityShanghai 200230China center for Genomic Research International Institutes of MedicineThe Fourth Affiliated HospitalZhejiang UniversityYiwu 322000China

Nonhuman primates(NHPs),our closest relatives,represent one of the most successful lineages of adaptive radiation in *** primate order contains several hundred species living in varied ecological niches with phenotypic ***,what we know about NHPs is still limited because of genome *** address this gap,the Primate Genome Project(PGP)consortium recently made a comprehensive sequencing effort.

关键词： evolution prima relatives

RNA nanostructure transformation into DNA ones

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Nano Research 2022年第10期15卷 9199-9203页

作者： Jiazhen Lyu Shiyu Ma Chong Zhang Zhen Huang Key Laboratory of bio-resource and Eco-environment of Ministry of Education College of Life SciencesSichuan UniversityChengdu 610065China The State Key Laboratory of biotherapy West China HospitalSichuan UniversityChengdu 610041China SeNA Research Institute Chengdu 610000China

RNAs and their assemblies can form diverse nano-structures and nano-shapes,offering various biological ***,by simply mimicking RNA sequences,DNAs cannot normally form the corresponding nanostructures,which makes the natureinspired transformations and designs *** understand the possible designs and connections between related RNA and DNA nano-shapes,herein we have reported several DNA squares transformed and derived from an RNA assembled square,by gradually replacing RNA with DNA *** have found that there were key RNA nucleotides:Their presences maintained the square,while their absences disrupted ***,we have revealed that as long as the conditions of higher ionic strengths or longer duplexes were included,the square RNA assembly could be completely replaced with DNA nucleotides,still offering the stable DNA *** experimental results have demonstrated that similar RNA nanostructure can be easily transformed into DNA ones via designing and increasing ionic strengths or duplex lengths.

关键词： RNA nanostructure DNA square assembly molecular transformation ionic strength duplex length

A new species of the genus Amolops (Anura： Ranidae) from high-altitude Sichuan, southwestern China, with a discussion on the taxonomic status of Amolops kangtingensis

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Zoological Research 2017年第3期38卷 138-145页

作者： Liang Fei Chang-Yuan Ye Yu-Fan Wang Ke Jiang Chengdu Institute of biology Chinese Academy of Sciences Chengdu Sichuan 610041 China State Key Laboratory of Genetic resources and Evolution Kunming Institute of Zoology Chinese Academy of Sciences KunmingYunnan 650223 China Zhejiang Forest resource Monitoring center Hangzhou Zhejiang 310020 China

A new species of the genus Amolops Cope, 1865 is described from Xinduqiao, Kangding, Sichuan. It was previously identified as Amolops kangtingensis, which is synonymized to Amolops mantzorum in this study. The new species, Amolops xinduqiao sp. nov., is distinguished from all other congeners by the following combination of characters：（1） medium body size, adult males SVL 41.2-47.5 mm （n=15, average 43.9 mm）, adult females SVL 48.5-56.6 mm （n=15, average 52.5 mm）; （2） head length equal to width or slightly wider than long; （3） tympanum small, but distinct; （4） vomerine teeth in two tiny rows, separated by a space about one vomerine teeth row; （5） bony projections on lower jaw absent; （6） dorsolateral folds usually absent; （7） tarsal folds or glands on tarsus absent; （8） circummarginal groove on disc of finger I absent; （9） tibiotarsal articulation reaching nostril or beyond; （10） webs of toe IV reaching to distal articulation, other toes fully webbed to disc; and （11） vocal sac absent in males.

关键词： New species Sichuan Taxonomy Amolops xinduqiao sp. nov. Amolops kangtingensis

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Back to Science in Searching for SARS-CoV-2 Origins

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China CDC weekly 2023年第14期5卷 315-317页

作者： William J Liu Wenwen Lei Xiaozhou He Peipei Liu Qihui Wang Zhiqiang Wu Yun Tan Shuhui Song Gary Wong Jian Lu Jingkun Jiang Qiang Wei Mingkun Li Juncai Ma Xiaozhong Peng Yixue Li Baoxu Huang Yigang Tong Jun Han Guizhen Wu NHC Key Laboratory of biosafety National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijingChina CAS Key Laboratory of Pathogen Microbiology and Immunology Institute of MicrobiologyChinese Academy of SciencesBeijingChina NHC Key Laboratory of Systems biology of Pathogens Institute of Pathogen BiologyChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijingChina Shanghai Institute of Hematology State Key Laboratory of Medical GenomicsNational Research Center for Translational MedicineRuijin Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghaiChina Beijing Institute of Genomics Chinese Academy of Sciencesand China National Center for BioinformationBeijingChina Laboratory of Molecular Virology and Immunology Institut Pasteur of ShanghaiChinese Academy of SciencesShanghaiChina State Key Laboratory of Protein and Plant Gene Research Center for BioinformaticsSchool of Life SciencesPeking UniversityBeijingChina School of Environment Tsinghua UniversityBeijingChina National Pathogen resource center Chinese Center for Disease Control and Prevention(China CDC)BeijingChina Microbial resource and Big Data center Chinese National Microbiology Data Center(NMDC)Institute of MicrobiologyChinese Academy of SciencesBeijingChina State Key Laboratory of Medical Molecular biology Department of Molecular Biology and BiochemistryInstitute of Basic Medical SciencesMedical Primate Research CenterNeuroscience CenterChinese Academy of Medical SciencesSchool of Basic MedicinePeking Union Medical CollegeBeijingChina School of Life Science and Technology ShanghaiTech UniversityShanghaiChina bio-Med Big Data center Key Laboratory of Computational BiologyCAS-MPG Partner Institute for Computational BiologyShanghai Institute of Nutrition and HealthUniversity of Chinese Academy of SciencesChinese Academy of SciencesShanghaiChina China Animal Health and Epidemiology center Qingdao CityShandong ProvinceChi

In recent decades,emerging and re-emerging human-infecting pathogens have been represented as huge threats to public health and have become a global concern(1).After outbreaks of two coronaviruses(CoVs),severe acute respiratory syndrome coronavirus(SARS-CoV)and Middle East respiratory syndrome coronavirus(MERS-CoV),severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)became the first-known pandemic hastening CoV with tremendous wrecking to the world(2).The origin tracing of these emerging pathogens is of great significance in infectious disease prevention and control(3–4).The origin of SARS-CoV-2 remains elusive after the more than 3-year pandemic,though scientists around the world are making great *** the experience of studying many other infectious pathogens,origin tracing is systematic and time-consuming *** supposed origins of many infectious pathogens are still in debate,including SARS-CoV and human immunodeficiency virus,etc(5).

关键词： prevention respiratory acute

Genomic consequences of population decline in critically endangered pangolins and their demographic histories

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National Science Review 2020年第4期7卷 798-814页

作者： Jing-Yang Hu Zi-Qian Hao Laurent Frantz Shi-Fang Wu Wu Chen Yun-Fang Jiang Hong Wu Wei-Min Kuang Haipeng Li Ya-Ping Zhang Li Yu State Key Laboratory for Conservation and Utilization of bio-resource in Yunnan School of Life Sciences Yunnan University State Key Laboratory of Genetic resources and Evolution Kunming Institute of Zoology Chinese Academy of Sciences Kunming College of Life Science University of Chinese Academy of Sciences CAS Key Laboratory of Computational biology CAS-MPG Partner Institute for Computational Biology Shanghai Institute of Nutrition and Health Chinese Academy of Sciences University of Chinese Academy of Sciences School of biological and Chemical Sciences Queen Mary University of London The Palaeogenomics and bio-Archaeology Research Network Department of Archaeology University of Oxford Guangzhou Zoo Lushui Management and Conservation Branch of Gaoligong Mountain National Nature Reserve center for Excellence in Animal Evolution and Genetics Chinese Academy of Sciences

Pangolins are among the most critically endangered animals due to heavy poaching and worldwide trafficking. However, their demographic histories and the genomic consequences of their recent population declines remain unknown. We generated high-quality de novo reference genomes for critically endangered Malayan(Manis javanica, MJ) and Chinese(M. pentadactyla, MP) pangolins and re-sequencing population genomic data from 74 MJs and 23 MPs. We recovered the population identities of illegally traded pangolins and previously unrecognized genetic populations that should be protected as evolutionarily distinct conservation units. Demographic reconstruction suggested environmental changes have resulted in a population size fluctuation of pangolins. Additionally, recent population size declines due to human activities have resulted in an increase in inbreeding and genetic load. Deleterious mutations were enriched in genes related to cancer/diseases and cholesterol homeostasis, which may have increased their susceptibility to diseases and decreased their survival potential to adapt to environmental changes and high-cholesterol diets. This comprehensive study provides not only high-quality pangolin reference genomes, but also valuable information concerning the driving factors of long-term population size fluctuations and the genomic impact of recent population size declines due to human activities, which is essential for pangolin conservation management and global action planning.

关键词： pangolins population genomics population declines genetic diversity inbreeding genetic load demographic history

Comparison and Correlation Study of Polar biomarkers of CKD Patients in Saliva and Serum by UPLC-ESI-MS

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Journal of Advances in Medicine Science 2019年第3期2卷 16-24页

作者： Jielian Yan Baihai Su Qiang Liu Li Zhou Xiaohong Tang Yong Liu Yixiang Duan Research center of Analytical Instrumentation Analytical & Testing Center Sichuan University Chengdu Sichuan 610064 China Department of Nephrology West China Hospital Sichuan University Chengdu Sichuan 610064 China Research center of Analytical Instrumentation Key Laboratory of Bio-resource and Eco-environment Ministry of Education College of Life Science Sichuan University Chengdu Sichuan 610064 China

There are abundant reports on the use of aromatic amino acids and creatinineas biomarkers in serum and urine forCKD(chronic kidney disease). However, investigationsinto these bio-species in bio-fluids, such as saliva and sweat, are rarely reported. Increasing interest in non-invasive methods for medical diagnosis advocates for the testing of these bio-fluidstoidentify potential biomarkers for prompt clinical andpreliminary screening using advanced analytical equipment. Unstimulated whole saliva samples were obtained from twenty-seven CKD patients and an equivalent number of healthy individuals. Saliva was assayed with ultra-performance liquid chromatography coupled with electro-spray ionization tandem mass spectrometry (UPLC-ESI-MS) in hydrophilic interaction chromatography mode. The data were analyzed using a student’s t test and receiver operatingcharacteristic (ROC) to evaluate the predictive power of several potential biomarkers (Pbiomarkers that demonstrated significant differences (Pbiomarkers in saliva between CKD patients and healthy people. This study explores the potential of CKD diagnosis by saliva, and demonstrates a positive correlation between salivary and serum creatinine.

关键词： UPLC MS CKD biomarkers