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A new 2-aminosteroid induces cellular differentiation and upregulates the expression of MafB and Egr-1 genes respectively in HL-60 and K562 leukemia cells

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Chinese Medical Journal 2005年第2期118卷 91-99页

作者： HEQun LIQiong YUANLin-bo HEJun researchlaboratoryofbloodphysiology DepartmentofPhysiologyXiangyaSchoolofMedicineCentralSouthUniversityChangsha410078China

Background In previous work, we suggested that some 2aminosteroids inhibited proliferation and induced differentiation of both human and murine leukemia cells Here, we reported the actions of another new 2aminosteroid designated as H89712 on human leukemia cells Methods Cell colony counting and MTT assay were used to determine proliferation Cell morphology, histochemical staining, UV detection and cytometry were used to determine differentiation RTPCR was used to detect gene expression. Standard statistical method was used to analyze dataResults H89712 inhibited proliferation of HL60 leukemia cells and the inhibition percentage in MTT assay was 18% at the dose of 108 mol/L and 65% at the dose of 105 mol/L, respectively The inhibition for HL60 in colony assay was 23% at the dose of 108 mol/L and 96% at the dose of 105 mol/L, respectively H89712 also induced HL60 cells toward macrophagelike differentiation It was verified by flow cytometry that the percentage of positive CD14 expression in differentiated HL60 cells was about 9 times higher than that of the control at the dose of 108 mol/L and 20 times higher than that of the control at the dose of 105 mol/L respectively, and this action involved upregulation of MafB gene in HL60 leukemia cells On the other hand, H89712 inhibited proliferation of K562 leukemia cells and the inhibition of K562 leukemia cells in MTT assay was shown by 34% at the dose of 108 mol/L and 88% at the dose of 105 mol/L respectively The inhibition of K562 leukemia cells in colony assay was 53% at the dose of 108 mol/L and 100% at the dose of 105 mol/L respectively H89712 also induced K562 cells toward erythroidlike diffe

关键词： 2-aminosteroid proliferation differentiation MafB Egr-1 HL-60 K562 leukemia

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