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Gelatin methacrylate hydrogel scaffold carrying resveratrol-loaded solid lipid nanoparticles for enhancement of osteogenic differentiation of BMSCs and effective bone regeneration

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Regenerative Biomaterials 2021年第5期8卷 120-133页

作者： Bangguo Wei Wenrui Wang Xiangyu Liu Chenxi Xu Yanan Wang Ziqi Wang Jinnuo Xu Jianzhong Guan Pinghui Zhou Yingji Mao Department of Orthopedics First Affiliated HospitalBengbu Medical CollegeBengbu 233004China Anhui Province Key Laboratory of Tissue Transplantation Bengbu Medical CollegeBengbu 233030China School of Life Sciences Bengbu Medical CollegeBengbu 233030China Anhui Province Key Laboratory of Translational Cancer research Bengbu Medical CollegeBengbu 233030China Department of Orthopedics Fuyang People‘s HospitalFuyang 236000China

Critical-sized bone defects caused by traumatic fractures,tumour resection and congenital malformation are unlikely to heal spontaneously.Bone tissue engineering is a promising strategy aimed at developing in vitro replacements for bone transplantation and overcoming the limitations of natural bone grafts.In this study,we developed an innovative bone engineering scaffold based on gelatin methacrylate(GelMA)hydrogel,obtained via a two-step procedure:first,solid lipid nanoparticles(SLNs)were loaded with resveratrol(Res),a drug that can promote osteogenic differentiation and bone formation;these particles were then encapsulated at different concentrations(0.01%,0.02%,0.04%and 0.08%)in GelMA to obtain the final Res-SLNs/GelMA scaffolds.The effects of these scaffolds on osteogenic differentiation of bone marrow mesenchymal stem cells(BMSCs)and bone regeneration in rat cranial defects were evaluated using various characterization assays.Our in vitro and in vivo investigations demonstrated that the different Res-SLNs/GelMA scaffolds improved the osteogenic differentiation of BMSCs,with the ideally slow and steady release of Res;the optimal scaffold was 0.02 Res-SLNs/GelMA.Therefore,the 0.02 Res-SLNs/GelMA hydrogel is an appropriate release system for Res with good biocompatibility,osteoconduction and osteoinduction,thereby showing potential for application in bone tissue engineering.

关键词： gelatin methacrylate bone marrow mesenchymal stem cells solid lipid nanoparticles resveratrol bone regeneration

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Prazosin:a potential new management tool for iatrogenic autonomic dysreflexia in individuals with spinal cord injury?

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Neural regeneration research 2015年第4期10卷 557-558页

作者： Mei M.Z.Zheng Aaron A.Phillips Stacy L.Elliott Andrei V.Krassioukov Experimental Medicine Program Faculty of MedicineUniversity of British ColumbiaVancouver V5Z 1M9Canada International Collaboration on Repair Discoveries (ICORD) University of British ColumbiaVancouver V5Z 1M9Canada Centre for Heart Lungand Vascular HealthFaculty of Health and Social DevelopmentUniversity of British ColumbiaKelowna V1V 1V7Canada Department of Psychiatry University of British Columbiaancouver V6T 2A1Canada Department of and Urologic Sciences University of British ColumbiaVancouver V5Z 1M9Canada Vancouver Sperm Retrieval Clinic Vancouver Coastal Health AuthorityVancouver V5Z 1M9Canada G.F.Strong Rehabilitation Center Sexual Health Rehabilitation ServiceVancouver V5Z 2G9Canada Division of Physical Medicine and Rehabilitation Department of MedicineUniversity of British ColumbiaVancouver V5Z 2G9Canada

Spinal cord injury （SCI） is a devastating condition that not only results in a loss of motor functions but also severe autonomic dysfunctions （Krassioukov and Claydon, 2006）. Autonomic dysreflexia （AD） is a life threatening episode of tran- sient hypertension that occurs up to 30x/day （1 Ix/day on average） in those with cervical or high thoracic SCI （Hubli et al., 2015）. Most common triggers of AD are from stimuli such as a full bowel and/or bladder, or sexual arousal （Teasell et al., 2000）. Penile vibrostimulation （PVS） is a clinical pro- cedure for sperm retrieval used for the purpose of family planning or fertility assessment that unfortunately iatrogenically induces episodes of AD （Elliott, 2006）. Recently, we published a clinical trial highlighting that prazosin may be a viable option for treating AD secondary to PVS （Phillips et al., 2014）.

关键词： Prazosin SCI AD DBP

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Novel Magnetically Interconnected Micro/Macroporous Structure of Monolithic Porous Carbon Adsorbent Derived from Sodium Alginate and Wasted Black Liquor and Its Adsorption Performance

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Journal of Renewable Materials 2021年第6期9卷 1059-1074页

作者： Parichart Onsri Decha Dechtrirat Patcharakamon Nooeaid Apiluck Eiad-ua Pongsaton Amornpitoksuk Supanna Techasakul Ahmad Taufiq Laemthong Chuenchom Division of Physical Science Faculty of SciencePrince of Songkla UniversitySongkhla90112Thailand Department of Materials Science Faculty of ScienceKasetsart UniversityBangkok10900Thailand Specialized Center of Rubber and Polymer Materials for Agriculture and Industry(RPM) Faculty of ScienceKasetsart UniversityBangkok10900Thailand Laboratory of Organic Synthesis Chulabhorn Research InstituteBangkok10210Thailand Division of Polymer Materials Faculty of Agricultural Product Innovation and TechnologySrinakharinwirot UniversityNakhon Nayok26120Thailand College of Nanotechnology King Mongkut’s Institute of Technology LadkrabangBangkok10520Thailand Center of Excellence for Innovation in Chemistry Faculty of SciencePrince of Songkla UniversitySongkhla90112Thailand Department of Physics Faculty of Mathematics and Natural SciencesUniversitas Negeri MalangMalang65145Indonesia

The novel and facile preparation of magnetically interconnected micro/macroporous structure of monolithic porous carbon adsorbent(MPCA)were designed and presented herein.The synthesis was achieved via conventional freezedrying and pyrolysis processes.In this study,sodium alginate and wasted black liquor were employed as starting precursors.Sodium alginate acts as a template of materials,whereas black liquor,the wasted product from the paper industry with plentiful of lignin content and alkaline solution,played an essential role in the reinforcement and activation of porosity for the resulting materials.Moreover,both the precursors were well dissolved in Fe^(3+) solution,providing a simple addition of a magnetic source in a one-pot synthesis.The interconnected micro/macroporous structures were generated through freeze-drying and,subsequently the pyrolysis process.The obtained cylindricalshaped monolithic porous carbon adsorbent(MPCA-700)showed high mechanical stability,a high BET specific surface area(902 m^(2)/g).Such aforementioned features were considered suitable to make the synthesized monolith as an adsorbent for the removal of heavy metal ions.The maximum adsorption capacity of MPCA-700 towards Pb^(2+) ions was 76.34 mg/g at pH 5.The adsorption studies illustrated that adsorption kinetics and isotherm perfectly fitted with the pseudo-second-order kinetics model and Langmuir isotherm,respectively.This work presents a promising protocol to reduce the overall costs in the preparation of renewable adsorbents with good adsorption efficiency and regeneration.

关键词： Hierarchical porous carbon monoliths magnetic properties adsorption alginate black liquor

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Abnormalcortical thickness in relapsing-remitting multiple sclerosis,correlations with cognition impairment,and effect of modified Bushenyisui decoction(补肾益髓汤)on cognitive function of multiple sclerosis

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Journal of Traditional Chinese Medicine 2021年第2期41卷 316-325页

作者： ZHAO Xuesong YANG Tao CHENG Fang YANG Song ZHU Wanlin LI Shaowu FAN Yongping Department of Traditional Chinese Medicine Beijing Tiantan HospitalCapital Medical UniversityBeijing 100070China Department of Anorectal Xiyuan HospitalChina Academy of Chinese Medical SciencesBeijing 100091China Institute of neurosurgery Institute of NeurosurgeryTiantan HospitalCapital Medical UniversityBeijing 100070China China National Clinical research Center for Neurological Diseases Beijing Tiantan HospitalCapital Medical UniversityBeijing 100070China

OBJECTIVE:To investigate the changes of subcortical gray matter volume and cortical thickness,andexplorethe correlations between regional abnormalities of cortical thickness and cognitive impairment and the effect of modified Bushenyisui decoction(补肾益髓汤,BSYSD)on the cognitive function of multiple sclerosis(MS).METHODS:This prospective study was approved by the institutional review board.92 subjects were recruited,including 46 relapsing-remitting multiple sclerosis(RRMS)patients and 46 healthy controls(HC).Of the 46 patients,22 patients experienced the treatment of BSYSD for half a year.A conventional three-dimensional T1-weighted sequence were acquired for all participants on a 3.0 tesla magnetic resonance system.Basic information,detailed cognitive scales Montreal Cognitive Assessment(MoCA),symbol digit modalities test(SDMT),immediate memory,delayed recall,and long-term recognition were evaluated.Subcortical gray matter volume and cortical thickness weremeasured by FreeSurfer.The correlations between cortical thickness which MS patients showed reduced with respect to HC and cognitive scales wereanalyzed by Pearson correlation in RRMS patients.The influence of modified BSYSD on MS patients'cognition was analyzed by paired T Test.RESULTS:MoCA,immediate memory,delayed recall,and long-term delayed recognition in RRMS were significantly decreased than those of HC.Gray matter atrophy measured by FreeSurfer showed mainly in thalamus and hippocampus of RRMS patients.Compared with HC,the cortical thickness of several regions in frontal lobe,parietal lobe,temporal lobe,hippocampal,cingulate gyrus,and fusiform gyrus of RRMS patients were decreased with significant difference.The regions of cortical thickness thinning related to MoCA,immediate memory,delayed recall,and long-term delayed recognition were temporal lobe and fusiform gyrus.Modified BSYSD could improve MoCA,SDMT,immediate memory,delayed recall,and long-term delayed recognition of MS patients,and it could promote the recovery of cognitive function in MS patients.

关键词： multiple sclerosis,relapsing-remitting cognitive dysfunction brain cortical thickness Gray matter Bushenyisui decoction

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Physiological and pathological insights into exosomes in the brain

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Zoological research 2020年第4期41卷 365-372页

作者： Xiao-Hui Li Juan Zhang Ding-Feng Li Wei Wu Zhong-Wen Xie Qiang Liu Institute on Aging and Brain Disorders The First Affiliated Hospital of USTCDivision of Life Sciences and MedicineHefei National Laboratory for Physical Sciences at the MicroscaleSchool of Life SciencesUniversity of Science and Technology of ChinaHefeiAnhui230001China State Key Laboratory of Tea Plant Biology and Utilization Anhui Agricultural UniversityHefeiAnhui 230036China Neurodegenerative Disease research Center University of Science and Technology of ChinaHefeiAnhui 230026China Organ Transplantation Center The First Affiliated Hospital of University of Science and Technology of ChinaHefeiAnhui 230026China CAS Center for Excellence in Animal Evolution and Genetics Chinese Academy of SciencesKunmingYunnan 650223China National Synchrotron Radiation Laboratory University of Science and Technology of ChinaHefeiAnhui 230029China CAS Key Laboratory of Brain Function and Disease University of Science and Technology of ChinaHefeiAnhui 230026China

Exosomes are small vesicles secreted by all cell types in the brain and play a role in cell-cell communication through the transfer of cargo or encapsulation.Exosomes in the brain have considerable impact on neuronal development,activation,and regeneration.In addition,exosomes are reported to be involved in the onset and propagation of various neurodegenerative diseases.In this review,we discuss the content of exosomes derived from major cell types in the brain,and their function under physiological and pathological conditions.

关键词： pathological Exosomes insight

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MicroRNAs as novel regulators of stem cell fate

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World Journal of Stem Cells 2013年第4期5卷 172-187页

作者： Eunmi Choi Ki-Chul Hwang Severance Biomedical Science Institute Yonsei University College of Medicine Severance Integrative research Institute for Cerebral & Cardiovascular Disease Yonsei University Health System

Mounting evidence in stem cell biology has shown that microRNAs(miRNAs) play a crucial role in cell fate specification, including stem cell self-renewal, lineagespecific differentiation, and somatic cell reprogramming.These functions are tightly regulated by specific gene expression patterns that involve miRNAs and transcription factors. To maintain stem cell pluripotency, specific miRNAs suppress transcription factors that promote differentiation, whereas to initiate differentiation, lineagespecific miRNAs are upregulated via the inhibition of transcription factors that promote self-renewal. Small molecules can be used in a similar manner as natural miRNAs, and a number of natural and synthetic small molecules have been isolated and developed to regulate stem cell fate. Using miRNAs as novel regulators of stem cell fate will provide insight into stem cell biology and aid in understanding the molecular mechanisms and crosstalk between miRNAs and stem cells.Ultimately, advances in the regulation of stem cell fate will contribute to the development of effective medical therapies for tissue repair and regeneration. This review summarizes the current insights into stem cell fate determination by miRNAs with a focus on stem cell self-renewal, differentiation, and reprogramming. Small molecules that control stem cell fate are also highlighted.

关键词： MicroRNA Stem cell fate Differentiation Self-renewal Reprogramming Small molecule

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PERIPHERAL NERVE regeneration:development OF A NOVEL NERVE CONDUIT TO DIRECT NERVE regeneration

PERIPHERAL NERVE REGENERATION:DEVELOPMENT OF A NOVEL NERVE C...

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The4thAsian-PacificOrgan...

作者： Hsin-Hsin Shen Jean-Dean Yang Hou-Yu Chiang Bin-Hong Tsai Hsiung-Fei Chien Sung-Tsang Hsieh Jui-Hsiang Chen Division of Biomaterials and Tissue Engineering Biomedical Engineering CenterIndustrial Technology Research Institute Division of Biomaterials and Tissue Engineering Biomedical Engineering CenterIndustrial Technology Research Institute Department of Anatomy and Cell Biology National Taiwan UniversityCollege of MedicineTaipei Division of Biomaterials and Tissue Engineering Biomedical Engineering CenterIndustrial Technology Research Institute Department of Surgery National Taiwan University HospitalTaipei Department of Anatomy and Cell Biology National Taiwan UniversityCollege of MedicineTaipei

＜正＞We have fabricated a bioresorbable nerve conduit with multi-channel structure to guide the axon outgrowth and prevent neuroma formation. The wall structure of this conduit was designed to be permeable to nutrien...

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Building Osteogenic Microenvironments with a Double-Network Composite Hydrogel for Bone Repair

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research 2023年第3期6卷 319-333页

作者： Jiaying Li Jinjin Ma Qian Feng En Xie Qingchen Meng Wenmiao Shu Junxi Wu Liming Bian Fengxuan Han Bin Li State Key Laboratory of Radiation Medicine and Protection School of Radiation Medicine and Protection and Interdisciplinary Sciences(RAD-X)Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education InstitutionsThe First Affiliated HospitalSuzhou Medical CollegeSoochow UniversitySuzhouJiangsu 215006China Orthopedic Institute Department of Orthopaedic SurgeryThe First Affiliated HospitalSuzhou Medical CollegeSoochow UniversitySuzhouJiangsuChina Key Laboratory of Biorheological Science and Technology Ministry of EducationCollege of BioengineeringChongqing UniversityChongqing 400044China Department of Biomedical Engineering University of StrathclydeGlasgowG11QEUK School of Biomedical Sciences and Engineering South China University of TechnologyGuangzhou International CampusGuangzhou 511442China National Engineering research Center for Tissue Restoration and Reconstruction South China University of TechnologyGuangzhou 510006China Guangdong Provincial Key Laboratory of Biomedical Engineering South China University of TechnologyGuangzhou 510006 China Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education South China University of TechnologyGuangzhou 510006 China

The critical factor determining the in vivo effect of bone repair materials is the microenvironment,which greatly depends on their abilities to promote vascularization and bone formation.However,implant materials are far from ideal candidates for guiding bone regeneration due to their deficient angiogenic and osteogenic microenvironments.Herein,a double-network composite hydrogel combining vascular endothelial growth factor(VEGF)-mimetic peptide with hydroxyapatite(HA)precursor was developed to build an osteogenic microenvironment for bone repair.The hydrogel was prepared by mixing acrylatedβ-cyclodextrins and octacalcium phosphate(OCP),an HA precursor,with gelatin solution,followed by ultraviolet photo-crosslinking.To improve the angiogenic potential of the hydrogel,QK,a VEGF-mimicking peptide,was loaded in acrylatedβ-cyclodextrins.The QK-loaded hydrogel promoted tube formation of human umbilical vein endothelial cells and upregulated the expression of angiogenesis-related genes,such as Flt1,Kdr,and VEGF,in bone marrow mesenchymal stem cells.Moreover,QK could recruit bone marrow mesenchymal stem cells.Furthermore,OCP in the composite hydrogel could be transformed into HA and release calcium ions facilitating bone regeneration.The double-network composite hydrogel integrated QK and OCP showed obvious osteoinductive activity.The results of animal experiments showed that the composite hydrogel enhanced bone regeneration in skull defects of rats,due to perfect synergistic effects of QK and OCP on vascularized bone regeneration.In summary,improving the angiogenic and osteogenic microenvironments by our double-network composite hydrogel shows promising prospects for bone repair.

关键词： composite loaded inductive

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Two-dimensional gel electrophoresis and surface-enhanced laser desorption ionization-time of flight-mass spectrometry for detection of protein expression profiles in the hippocampus following closed brain injury

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Neural regeneration research 2010年第23期5卷 1795-1801页

作者： Qingming Shu Zhiqiang Li Shuwang Yang Lingzhi Li Xiao Bai Yongliang Zhang The Chinese PLA Postgraduate Medical School Beijing 100853 China Department of Pathology General Hospital of Chinese People's Armed Police Forces Beijing 100039 China Second Department of Internal Medicine Inner Mongolia Autonomous Regional Corps Hospital Huhhot 010031 Inner Mongolia Autonomous Region China Postgraduate Division Training Department of Medical College of Chinese People's Armed Police Forces Tianjin 300162 China Department of Pharmacochemistry Medical College of Chinese People's Armed Police Forces Tianjin 300162 China First Division South Building General Hospital of Chinese People's Armed Police Forces Beijing 100039 China Tianjin Key Laboratory for Biomarkers of Occupational and Environmental Hazard Department of Scientific Research Medical College of Chinese People's Armed Police Forces Tianjin 300162 China

Gene expression profile changes in brain regions following traumatic brain injury at the gene level cannot sufficiently elucidate gene expression time, expression amount, protein post-translational processing or modification. Therefore, it is necessary to quantitatively analyze the gene expression profile using proteomic techniques. In the present study, we established a rat model of closed brain injury using Marmarou＇s weight-drop device, and investigated hippocampal differential protein expression using two-dimensional gel electrophoresis and surface-enhanced laser desorption ionization-time of flight-mass spectrometry. A total of 364 protein peaks were detected on weak cation exchange-2 protein chips, including 37 differential protein peaks. 345 protein peaks were detected on immobilized metal affinity capture arrays-Cu, including 12 differential protein peaks Further examination of these differential proteins revealed that glucose-regulated protein and proteasome subunit alpha type 3 expression were significantly upregulated post-injury. These results indicate that brain injury can alter protein expression in the hippocampus, and that glucose-regulated protein and proteasome subunit alpha type 3 are closely associated with the occurrence and development of traumatic brain injury.

关键词： surface-enhanced laser desorption ionization-time of flight-mass spectrometry two-dimensional gel electrophoresis hippocampus proteomics brain injury neural regeneration

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NT-3促进CMT1A小鼠模型和患者的神经再生和感觉功能改善

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世界核心医学期刊文摘(神经病学分册) 2006年第1期 35-36页

作者： Sahenk Z. Nagaraja H.N. McCracken B.S. 周永 Columbus Children’s research Institute Molecular Medicine Division 700 Children’s Drive Columbus OH 43205 united States Dr.

Background: Xenografts from patients with Charcot-Marie-tooth type 1A (CMT1A) have shown delayed myelination and impaired regeneration of nude mice axons passing through the grafted segments. Neurotrophin-3 (NT-3), an important component of the Schwann cell (SC) autocrine survival loop, could correct these deficiencies. Objective: To assess the efficacy of NT-3 treatment in preclinical studies using animal models of CMT1A and to conduct a double-blind, placebo-controlled, randomized, pilot clinical study to assess the efficacy of subcutaneously administered NT-3 in patients with CMT1A. Methods: Nude mice harboring CMT1A xenografts and TremblerJ mice with a peripheral myelin protein 22-point mutation were treated with NT-3, and the myelinated fiber (MF) and SC numbers were quantitated. Eight patients received either placebo (n = 4) or 150 μg/kg NT-3 (n = 4) three times a week for 6 months. MF regeneration in sural nerve biopsies before and after treatment served as the primary outcome measure. Additional endpoint measures included the Mayo Clinic Neuropathy Impairment Score (NIS), electrophysiologic measurements, quantitative muscle testing, and pegboard performance. Results: The NT-3 treatment augmented axonal regeneration in both animal models. For CMT1A patients, changes in the NT-3 group were different from those observed in the placebo group for the mean number of small MFs within regeneration units (p=0.0001), solitary MFs, (p=0.0002), and NIS (p= 0.0041). Significant improvements in these variables were detected in the NT-3 group but not in the placebo group. Pegboard performance was significantly worsened in the placebo group. NT-3 was well tolerated. Conclusion: Neurotrophin-3 augments nerve regeneration in animal models for CMT1A and may benefit patients clinically, but these results need further confirmation.

关键词：患者神经再生 NT-3 CMT1A

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