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Gene Expression and Fungal Morphology in Submerged Culture Using Whole Barley of Aspergillus kawachii

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Journal of Agricultural Science and Technology(A) 2014年第1期4卷 13-26页

作者： Tadahiro Sunagawa Susumu Masuda Hiroshi Shoji research & Development laboratories for brewing Asahi Breweries Ltd. Moriya lbaraki 302-0106 Japan

The authors described a novel submerged batch culture system that produced high levels of amylase by Aspergillus kawachii using whole barley （WB）, the surface of which is covered by its husk. In this study, detailed analyses determining the amylase activities, residual sugars, fungal morphology and expression levels of genes were performed in a submerged culture using WB to address the mechanism underlying high amylase productivity in A. kawachii. High levels of glucoamylase and acid-stable u-amylase were produced in this culture, and expression levels of amylases, as well as glucose-repressive genes including high-affinity glucose transporter and peroxidase/catalase were also high. On the other hand, the morphology of mycelia was altered, with swollen, bulbous, multi-septum hyphae and conidiophores that normally form in a solid culture being partially generated. Furthermore, cell cycle and post-translational modification-related gene expression levels were altered, and were similar to those in the solid culture. These findings suggest that high amylase productivity in the submerged culture using WB is accompanied by both the up-regulation of amylase genes and activation of post-translational modifications due to fungal morphological changes being brought closer to those in the solid culture.

关键词： Aspergillus kawachii amylase submerged culture whole barley fungal morphology post-translational modification.

Antithrombotic Effects of Different Strains of Lactic Acid Bacteria

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Health 2014年第18期6卷 2433-2443页

作者： Hiromasa Ooe Masaru Kato Kanae Hyodo Keiko Nakashima Hiroshi Ashigai Kyoko Kato Toshinori Sasaki Yuko Fukushima John C. Giddings Junichiro Yamamoto research laboratories for Health Science and Food technologies Kirin Company Limited Yokohama Japan research laboratories for brewing technologies Kirin Company Limited Yokohama Japan Laboratory of Physiology Faculty of Nutrition Kobe Gakuin University Kobe Japan Central laboratories for Key technologies Kirin Company Limited Yokohama Japan Formerly Department of Haematology School of Medicine Cardiff University UK

Objective: The aim of the present study was to assess the antithrombotic properties of different strains of orally available antithrombotic lactic acid bacteria (LAB). research Methods & Procedures: Antithrombotic activity, antiplatelet reactivity and/or thrombolytic activity, were measured in seven strains of LAB and LAB cell-free-extracts (LAB filtrates) using the shear-induced platelet reactivity/thrombolytic activity, The Global Thrombosis Test (GTT), with non-anticoagulated rat blood in vitro. Subsequently, the most potent antithrombotic strains identified in vitro were assessed in vivo after oral administration in mouse carotid arteries using a helium-neon laser-induced thrombosis model. Result: Five strains out of seven LAB (Lactobacillus paracasei KW 3100, Lactobacillus fermentum NBRC 3961, Lactobacillus pentosus JCM 8333, Leuconostoc oeni Elios 1, Pediococcus pentosaceus NK-2) promoted significant endogenous thrombolysis in vitro. In addition, one strain (Lactobacillus fermentum NBRC 3961) significantly inhibited shear-induced platelet re-activity. Three antithrombotic strains, Lactobacillus fermentum NBRC 3961, Leuconostoc oeni Elios 1, and Lactobacillus pentosus JCM 8333, were further assessed in vivo. The results demonstrated that filtrates, and the cells per se of these LAB, modulated antiplatelet activity and/or thrombolytic activity, and that the antithrombotic mechanisms were mainly influenced by protein content (60% - 70% of dry matter). Conclusion: The findings suggested that some strains of lactic acid bacteria could usefully provide the basis for the production of oral antithrombotic probiotics.

关键词： Antithrombotic Lactic Acid Bacteria Shear-Induced Thrombosis Global Thrombosis Test (GTT) Laser-Induced Thrombosis Endogenous Thrombolysis

Blockchain-enabled wireless communications: a new paradigm towards 6G

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National Science Review 2021年第9期8卷 167-191页

作者： Jiaheng Wang Xintong Ling Yuwei Le Yongming Huang Xiaohu You National Mobile Communications research Laboratory Southeast University Purple Mountain laboratories

With the deployment of fifth-generation(5 G) wireless networks worldwide, research on sixth-generation(6 G) wireless communications has commenced. It is expected that 6 G networks can accommodate numerous heterogeneous devices and infrastructures with enhanced efficiency and security over diverse,e.g. spectrum, computing and storage, resources. However, this goal is impeded by a number of trust-related issues that are often neglected in network designs. Blockchain, as an innovative and revolutionary technology that has arisen in the recent decade, provides a promising solution. Building on its nature of decentralization, transparency, anonymity, immutability, traceability and resiliency, blockchain can establish cooperative trust among separate network entities and facilitate, e.g. efficient resource sharing,trusted data interaction, secure access control, privacy protection, and tracing, certification and supervision functionalities for wireless networks, thus presenting a new paradigm towards 6 G. This paper is dedicated to blockchain-enabled wireless communication technologies. We first provide a brief introduction to the fundamentals of blockchain, and then we conduct a comprehensive investigation of the most recent efforts in incorporating blockchain into wireless communications from several aspects. Importantly, we further propose a unified framework of the blockchain radio access network(B-RAN) as a trustworthy and secure paradigm for 6 G networking by utilizing blockchain technologies with enhanced efficiency and *** critical elements of B-RAN, such as consensus mechanisms, smart contract, trustworthy access,mathematical modeling, cross-network sharing, data tracking and auditing and intelligent networking, are elaborated. We also provide the prototype design of B-RAN along with the latest experimental results.

关键词： blockchain wireless communication 6G

Density-based user clustering in downlink NOMA systems

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Science China(Information Sciences) 2022年第5期65卷 196-204页

作者： Hanliang YOU Yaoyue HU Zhiwen PAN Nan LIU National Mobile Communication research Laboratory Southeast University Purple Mountain laboratories

Non-orthogonal multiple access(NOMA) technology, which can effectively improve the bandwidth utilization, is one of the key technologies in the next-generation wireless communication systems. In the downlink multiple antenna NOMA systems, user clustering is one of the problems that must be *** this paper, we focus on the user clustering that maximizes the system sum rate. First, a user clustering method based on the density-based spatial clustering of applications with noise(DBSCAN) algorithm is proposed for static user scenarios. Then an improved low-complexity dynamic clustering method is further developed for dynamic user scenarios. Simulation results show that compared with existing clustering methods, the DBSCAN-based method has better clustering performance in complex static user scenarios, and the proposed dynamic clustering method performs close to completely re-executing the DBSCAN-based method but with lower complexity.

关键词： NOMA user clustering machine learning DBSCAN dynamic clustering

An overview on integrated localization and communication towards 6G

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Science China(Information Sciences) 2022年第3期65卷 5-50页

作者： Zhiqiang XIAO Yong ZENG National Mobile Communications research Laboratory Southeast University Purple Mountain laboratories

While the fifth generation(5 G) cellular system is being deployed worldwide, researchers have started the investigation of the sixth generation(6 G) mobile communication networks. Although the essential requirements and key usage scenarios of 6 G are yet to be defined, it is believed that 6 G should be able to provide intelligent and ubiquitous wireless connectivity with Terabits per second(Tbps) data rate and submillisecond(sub-ms) latency over three-dimensional(3 D) network coverage. To achieve such goals, acquiring accurate location information of the mobile terminals is becoming extremely useful, not only for locationbased services but also for improving wireless communication performance in various ways such as channel estimation, beam alignment, medium access control, routing, and network optimization. On the other hand, the advancement of communication technologies also brings new opportunities to greatly improve the localization performance, as exemplified by the anticipated centimeter-level localization accuracy in 6 G by extremely large-scale multiple-input multiple-output(MIMO) and millimeter wave(mm Wave) *** this regard, a unified study on integrated localization and communication(ILAC) is necessary to unlock the full potential of wireless networks for dual purposes. While there are extensive studies on wireless localization or communications separately, the research on ILAC is still in its infancy. Therefore, this article aims to give a tutorial overview on ILAC towards 6 G wireless networks. After a holistic survey on wireless localization basics, we present the state-of-the-art results on how wireless localization and communication inter-play with each other in various network layers, together with the main architectures and techniques for localization and communication co-design in current two-dimensional(2 D) and future 3 D networks with aerial-ground integration. Finally, we outline some promising future research directions for ILAC.

关键词： wireless localization integrated localization and communication cellular networks B5G 6G

Modulating p-type doping of two-dimensional material palladium diselenide

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Nano research 2024年第4期17卷 3232-3244页

作者： Jiali Yang Yu Liu En-Yang Wang Jinbo Pang Shirong Huang Thomas Gemming Jinshun Bi Alicja Bachmatiuk Hao Jia Shu-Xian Hu Chongyun Jiang Hong Liu Gianaurelio Cuniberti Weijia Zhou Mark H Rümmeli Institute for Advanced Interdisciplinary research(iAIR) University of JinanJinan 250022China Institute for Materials Chemistry Leibniz Institute for Solid State and Materials Research Dresden(IFW Dresden)20 Helmholtz StrasseDresden 01069Germany State Key Lab of Transducer Technology Shanghai Institute of Microsystem and Information TechnologyChinese Academy of SciencesShanghai 200050China College of Energy Soochow Institute for Energy and Materials Innovations Soochow UniversitySuzhou 215006China Key Laboratory of Advanced Carbon Materials and Wearable Energy technologies of Jiangsu Province Soochow UniversitySuzhou 215006China School of Integrated Circuits University of Chinese Academy of SciencesBeijing 101408China Institute of Microelectronics of Tianjin Binhai New Area Tianjin 300451China Institute of Microelectronics Chinese Academy of SciencesBeijing 100029China Lukasiewicz research Network PORT Polish Center for Technology DevelopmentStablowicka 147Wroclaw 54-066Poland School of Mathematics and Physics University of Science and Technology BeijingBeijing 100083China College of electronic information and optical engineering Nankai UniversityTianjin 300350China Centre of Polymer and Carbon Materials Polish Academy of SciencesM.Curie Sklodowskiej 34Zabrze 41-819Poland Center for Energy and Environmental technologies VŠB-Technical University of Ostrava17.Listopadu 15Ostrava 70833Czech Republic State Key Laboratory of Crystal Materials Center of Bio&Micro/Nano Functional MaterialsShandong UniversityJinan 250100China Dresden Center for Computational Materials Science Technische Universität DresdenDresden 01062Germany Dresden Center for Intelligent Materials(GCL DCIM) Technische Universität DresdenDresden 01062Germany Institute for Materials Science and Max Bergmann Center of Biomaterials Technische Universität DresdenDresden 01069Germany Center for Advancing Electronics Dresden Technische Universität DresdenDresden 01069Germany

The van der Waals heterostructures have evolved as novel materials for complementing the Si-based semiconductor ***-10 noble metal dichalcogenides(e.g.,PtS_(2),PtSe_(2),PdS_(2),and PdSe_(2))have been listed into two-dimensional(2D)materials toolkit to assemble van der Waals *** them,PdSe_(2) demonstrates advantages of high stability in air,high mobility,and wide tunable ***,the regulation of p-type doping of PdSe_(2) remains unsolved problem prior to fabricating p–n junction as a fundamental platform of semiconductor ***,a quantitative method for the controllable doping of PdSe_(2) is yet to be *** this study,the doping level of PdSe_(2) was correlated with the concentration of Lewis acids,for example,SnCl_(4),used for *** the transfer characteristics,the threshold voltage(the gate voltage corresponding to the minimum drain current)increased after SnCl_(4) soaking ***_(2) transistors were soaked in SnCl_(4) solutions with five different *** threshold voltages from the as-obtained transfer curves were extracted for linear fitting to the threshold voltage versus doping concentration correlation *** study provides in-depth insights into the controllable p-type doping of PdSe_(2).It may also push forward the research of the regulation of conductivity behaviors of 2D materials.

关键词： two-dimensional(2D)materials Lewis acid treatment p-type doping field-effect transistors transfer characteristic

Full-spectrum cell-free RAN for 6G systems: system design and experimental results

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Science China(Information Sciences) 2023年第3期66卷 80-93页

作者： Dongming WANG Xiaohu YOU Yongming HUANG Wei XU Jiamin LI Pengcheng ZHU Yanxiang JIANG Yang CAO Xinjiang XIA Ziyang ZHANG Qingji JIANG Pan WANG Dongjie LIU Kang ZHENG Mengting LOU Jing JIN Qixing WANG Jiangzhou WANG National Mobile Communications research Laboratory Southeast University Purple Mountain laboratories China Mobile research Institute School of Engineering University of Kent

With distributed transceivers and scalable cooperative transmission, cell-free massive multipleinput multiple-output(CF-m MIMO) technology eliminates the “cells” of traditional mobile communications,thereby significantly improving the performance of wireless networks. CF-m MIMO is considered as a major technical tool for achieving the key performance indicators of sixth generation(6G) mobile communication systems. In this paper, a scalable implementation architecture for CF-m MIMO is proposed, based on which potential key technologies such as channel information acquisition, transceiver design, dynamic user and access point association, and novel duplexing are introduced. Finally, based on low-cost commercial access points, a CF-m MIMO testbed with 64 transmit and receive antennas is developed, and the performance of the distributed receiver design is evaluated.

关键词： 6G scalable cell-free massive MIMO cell-free radio access network prototype system

Receding-Horizon Trajectory Planning for Under-Actuated Autonomous Vehicles Based on Collaborative Neurodynamic Optimization

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IEEE/CAA Journal of Automatica Sinica 2022年第11期9卷 1909-1923页

作者： Jiasen Wang Jun Wang Qing-Long Han IEEE the Future Network research Center Purple Mountain LaboratoriesNanjing 211111China the Department of Computer Science the School of Data ScienceCity University of Hong KongHong KongChina the School of Science Computing and Engineering TechnologiesSwinburne University of TechnologyMelbourne VIC 3122Australia

This paper addresses a major issue in planning the trajectories of under-actuated autonomous vehicles based on neurodynamic optimization.A receding-horizon vehicle trajectory planning task is formulated as a sequential global optimization problem with weighted quadratic navigation functions and obstacle avoidance constraints based on given vehicle goal *** feasibility of the formulated optimization problem is guaranteed under derived *** optimization problem is sequentially solved via collaborative neurodynamic optimization in a neurodynamics-driven trajectory planning method/*** results with under-actuated unmanned wheeled vehicles and autonomous surface vehicles are elaborated to substantiate the efficacy of the neurodynamics-driven trajectory planning method.

关键词： Collaborative neurodynamic optimization receding-horizon planning trajectory planning under-actuated vehicles

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Practical active antenna evaluation using the two-stage MIMO OTA measurement method

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Practical active antenna evaluation using the two-stage MIMO...