检索结果-南通市图书馆

Increased expression of the nitric oxide synthase gene and protein in corpus cavernosum by repeated dosing of udenafil in a rat model of chemical diabetogenesis

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Asian Journal of Andrology 2009年第4期11卷 435-442页

作者： GJ Ahn HK Chung CH Lee KK Kang BO Ahn research Laboratory Dong-A Pharmaceutical Company Kiheung Yongin Kyunggi 446-905 Korea

Erectile dysfunction （ED） is a major complication of diabetes mellitus （DM）. This study investigates the relationship between ED and the downregulation of constitutive nitric oxide synthase （cNOS） in the corpus cavernosum （CC） of diabetic rats. It also examines the effects of udenafil, a phosphodiesterase type 5 （PDE5） inhibitor, on ED and cNOS expression levels. After 16 weeks of daily oral treatment with udenafil in diabetic rats, the intracavernous pressure/mean arterial pressure （ICP/MAP） ratio was recorded to measure erectile function, and cNOS expression was measured using reverse transcriptase （RT）-PCR and immunoblots. Although the ICP/ MAP ratio and the expression levels of endothelial NOS （eNOS） and neuronal NOS （nNOS） in the CC were markedly decreased in diabetic rats, long-term udenafil treatment improved the erectile function and increased cNOS expression compared with diabetic controls. These findings suggest that ED in DM is closely related to decreased cNOS expression in the CC and that udenafil has the ability to compensate for this pathological change by modulating cNOS expression. Udenafil also has an inhibitory role in cyclic guanosine monophosphate （cGMP） degradation.

关键词： cNOS diabetes mellitus erectile dysfunction udenafil

Erectile potentials of a new phosphodiesterase type 5 inhibitor,DA-8159,in diet-induced obese rats

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Journal of Andrology 2006年第3期8卷 325-329,388页

作者： Jae-YoungYu Kyung-KooKang MoohiYoo research institutes of dong a pharmaceutical company KiheungYounginKyunggi 449-905Korea

Aim:To examine the changes in the erectile function in diet-induced obese rats and investigate the oral efficacy of DA-8159,a new phosphodiesterase type 5(PDE5)inhibitor,on penile erection in obese ***:The rats were fed a high-energy diet for 12 weeks and divided into three groups:an obesity-resistant(OR)control group,an obesity- prone(OP)control group,and an OP-DA-8159 treatment(DA-8159)*** electrostimulation-induced erectile responses were measured in all *** body weight,plasma cholesterol,triglyceride and glucose levels were also ***:In the OP control group,the maximum intracavernous pressure(ICP)and ICP/blood pressure(ICP/BP)ratio after electric stimulation were significantly lower than those in OR control *** corresponding area under the curve(AUC)of the ICP/BP ratio,the detumescence time and the baseline cavernous pressure were also lower than those in the OR control group,but this difference was not *** body weight gain,plasma cholesterol and triglyceride level in the OP group were significantly higher than those in the OR group. After administering the DA-8159,a significant increase in the maximum ICP and the ICP/BP ratio were *** coerrsponding AUCs in the DA-8159 group were also higher than those in the two control ***,the detumescence time was significantly prolonged after treatment with ***:These results demon- strate that diet-induced obesity affects the erectile function in rats and these erectile dysfunction(ED)can be im- proved by the treatment with DA-8159,indicating DA-8159 might be a treatment option for ED associated with obesity.

关键词： phosphodiesterase type 5 inhibitor penile erection obesity DA-8159 intracavernous pressure

Melissa officinalis and rosmarinic acid in management of memory functions and Alzheimer disease

在线全文

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Biomedicine 2019年第2期9卷 47-52页

作者： Mohaddese Mahboubi Medicinal Plants research Department Research and DevelopmentTabibDaru Pharmaceutical Company

Alzheimer disease(AD) as worldwide progressive neurodegenerative disease is prevalent disease among elderly population. Due to limitation in chemical drugs along with their adverse effects of these treatments, research for finding more effective natural drugs, is one of interesting subjects among the scientists. Melissa officinalis(M. officinalis) has a long history of use in treatment of nervous system diseases. This review article evaluates the potency of M. officinalis in treatment of AD by review of experimental and clinical documents on the efficacy, safety and its mechanisms of action in management of AD. The information is extracted from electronic resources(PubMed, Wiley, Springer and Science Direct), English and Persian scientific books. In spite of different scientific and non-scientific reports on the use of M. officinalis and its main component of rosmarinic acid in neurodegenerative diseases, there is only one clinical trial on the efficacy of M. officinalis ethanol extract in management of AD. Different mechanisms of action for M. officinalis, including inhibitory effects against amyloid beta, reactive oxygen species, and acetylcholine esterase, are involved. Larger clinical trials are recommended to confirm the efficacy and safety of M. officinalis extracts in treatment of AD patients.

关键词： Lemon balm Melissa officinalis Alzheimer Acetylcholine Rosmarinic acid

Hepatoprotection by dandelion (Taraxacum officinale) and mechanisms

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Biomedicine 2020年第1期10卷 1-10页

作者： Mohaddese Mahboubi Mona Mahboubi Medicinal Plants research Department Research and DevelopmentTabibDaru Pharmaceutical CompanyKashanIran

The protection of the liver as an essential organ in the body against oxidative stress and deleterious compounds has been the subject of recent *** different compounds,medicinal plants play an important role due to their hepatoprotective *** officinale or"common dandelion"is a popular plant that has been traditionally used for its hepatoprotective ***,there are limited clinical studies on its hepatoprotective *** aim of this review article is to evaluate the hepatoprotective effects of dandelion and its mechanism of *** reviewed literature up to July 2019 on"Taraxacum officinale"or"dandelion"and *** available pharmacological studies indicate that dandelion extracts have hepatoprotective effects against chemical agents due to its antioxidant and antiinflammatory *** anti-inflammatory effects of dandelion,the prebiotic effects of its oligofructans,inhibitory effects against the release of lipopolysaccharides and fasting induced adipose factor,digestive enzymes,and enhancing effects of lipogenesis,reduce lipid accumulation and liver inflammation,which directly or indirectly improve the liver *** emerging evidence on hepatoprotective effects of dandelion,designing large human clinical studies is essential.

关键词： Dandelion Hepatoprotective effects Antioxidant Anti-inflammatory

Therapeutic effect of DA-9601 on chronic reflux gastritis induced by sodium taurocholate in rats

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2005年第47期11卷 7430-7435页

作者： Tae Young Oh Chang Yell Shin Yong Sung Sohn dong Hwan Kim Byoung Ok Ahn Eun Bang Lee Cho Hyun Park dong-A pharmaceutical research Institute YonginKorea Department of General Surgery Catholic University of MedicineSeoulKorea

AIM: To investigate the therapeutic effects of DA-9601 on sodium taurocholate (TCA)-induced chronic reflux gastritis in SD ***: In this study, we have investigated the therapeutic effects of DA-9601 on chronic erosive and atrophic gastritis induced by 6 mo of TCA administration (5 mmol/L in drinking water) in SD rats. RESULTS: Four weeks of DA-9601 administration (0.065%, 0.216% in rat chow), following the withdrawal of TCA treatment, resulted in a significant decrease in total length of erosions in rats in a dose-dependent manner. Furthermore, the indicators of atrophic gastritis, such as reduced mucosal thickness and reduction in the number of parietal cells, were improved by the administration of DA-9601 in a dose-related manner. DA-9601 also attenuated inflammatory cell infiltration and the proliferation of collagenous fiber in the gastric mucosa. The improvement in the reduction of the gastric mucus was observed in the rats receiving a high dose of DA-9601 (0.216%). The therapeutic effect of DA-9601 on experimental chronic erosive gastritis was superior to that of rebamipide (1.08% in rat chow). Biochemical analyses showed increased mucosal prostaglandin E2 and reduced glutathione levels by DA-9601 treatment. CONCLUSION: We suggest that DA-9601 is apromising agent for the treatment of chronic erosive and atrophic gastritis with an etiological factor of bile reflux. Increasedmucosal prostaglandin E2 and reduced glutathione by DA-9601 treatment may be therapeutic mechanisms for chronic erosive and atrophic gastritis.

关键词： DA-9601 Reflux gastritis Erosive gastritis Atrophic gastritis Sodium taurocholate

Single-cell omics: experimental workflow, data analyses and applications

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China Life Sciences 2025年第1期68卷 5-102页

作者： Fengying Sun Haoyan Li dongqing Sun Shaliu Fu Lei Gu Xin Shao Qinqin Wang Xin dong Bin Duan Feiyang Xing Jun Wu Minmin Xiao Fangqing Zhao Jing-dong J.Han Qi Liu Xiaohui Fan Chen Li Chenfei Wang Tieliu Shi Department of Clinical Laboratory the Affiliated Wuhu Hospital of East China Normal University (The Second People's Hospital of Wuhu City) pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang University Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration (Tongji University) Ministry of EducationOrthopaedic DepartmentTongji HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Frontier Science Center for Stem Cells School of Life Sciences and TechnologyTongji University Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine Shanghai East HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University research Institute of Intelligent Computing Zhejiang Lab Shanghai research Institute for Intelligent Autonomous Systems Center for Single-cell Omics School of Public HealthShanghai Jiao Tong University School of Medicine National Key Laboratory of Chinese Medicine Modernization Innovation Center of Yangtze River DeltaZhejiang University Center for Bioinformatics and Computational Biology Shanghai Key Laboratory of Regulatory Biologythe Institute of Biomedical Sciences and School of Life SciencesEast China Normal University Beijing institutes of Life Science Chinese Academy of Sciences Peking-Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary StudiesCenter for Quantitative Biology (CQB)Peking University Zhejiang Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases Women's HospitalZhejiang University School of Medicine Key Laboratory of Advanced Theory and Application in Statistics and Data Science-MOE School of StatisticsEast China Normal University

Cells are the fundamental units of biological systems and exhibit unique development trajectories and molecular features. Our exploration of how the genomes orchestrate the formation and maintenance of each cell, and control the cellular phenotypes of various organismsis, is both captivating and intricate. Since the inception of the first single-cell RNA technology, technologies related to single-cell sequencing have experienced rapid advancements in recent years. These technologies have expanded horizontally to include single-cell genome, epigenome,proteome, and metabolome, while vertically, they have progressed to integrate multiple omics data and incorporate additional information such as spatial sc RNA-seq and CRISPR screening. Single-cell omics represent a groundbreaking advancement in the biomedical field,offering profound insights into the understanding of complex diseases, including cancers. Here, we comprehensively summarize recent advances in single-cell omics technologies, with a specific focus on the methodology section. This overview aims to guide researchers in selecting appropriate methods for single-cell sequencing and related data analysis.

关键词： single-cell sequencing genome epigenome proteomics metabolomics multimodal spatial transcriptomics CRISPR screening

Syntheses and biological activities of chiral piperidines-tachykinin NK3 antagonists

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

中国药理学报 1999年第3期20卷 283-288页

作者：陈怀谷钟富荣郭必胜余承慧 Parke Davis pharmaceutical research Division Warner Lambert Company 美国 481 0 5

ＩＮＴＲＯＤＵＣＴＩＯＮＯｖｅｒｔｈｅｌａｓｔｄｅｃａｄｅ，ｍａｊｏｒａｄｖａｎｃｅｓｈａｖｅｂｅｎｍａｄｅｉｎｕｎｄｅｒｓｔａｎｄｉｎｇｔｈｅｂｉｏｌｏｇｙｏｆｍａｍｍａｌｉａｎｔａｃｈｙｋｉｎｉｎｎｅｕｒｏｐｅｐ... 详细信息

ＩＮＴＲＯＤＵＣＴＩＯＮＯｖｅｒｔｈｅｌａｓｔｄｅｃａｄｅ，ｍａｊｏｒａｄｖａｎｃｅｓｈａｖｅｂｅｎｍａｄｅｉｎｕｎｄｅｒｓｔａｎｄｉｎｇｔｈｅｂｉｏｌｏｇｙｏｆｍａｍｍａｌｉａｎｔａｃｈｙｋｉｎｉｎｎｅｕｒｏｐｅｐｔｉｄｅｓ．Ｉｔｉｓｎｏｗｗｅｌｅ...

关键词：哌啶类速激肽神经激肽K 立体异构构效关系

Effect of age on the pharmacokinetics of polymorphic nimodipine in rats after oral administration

在线全文

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2016年第5期6卷 468-474页

作者： Wenli Liu Xiaona Wang Ruilian Chen Kaixuan Zhang Yao Li Yi Li Duanyun Si Junbo Gong Dianshu Yin Yongli Wang Zhenping Wei Mingshi Yang School of Chemical Engineering and Technology Tianjin University State Key Laboratory of Drug Delivery Technology and Pharmacokinetics Tianjin Institute of Pharmaceutical Research pharmaceutical research Institute Shijiazhuang No.4 Pharmaceutical Company Faculty of pharmaceutical Science University of Copenhagen

The previous investigation has proved that their existed pharmacokinetic difference between the different crystal forms of the polymorphic drugs after oral ***,no systemic investigations have been made on the change of this pharmacokinetic difference,resulted either from the physiological or from the pathological *** this paper,we used polymorphic nimodipine(Nim) as a model drug and investigated the effect of age difference(2- and 9-month old) on the pharmacokinetics after oral delivery in *** the results shown,for L-form of Nim(L-Nim),the AUC0–24 hin 2-month-old rats was 343.68747.15 ng h/m L,which is 23.36% higher than that in 9-month-old *** H-form of Nim(H-Nim),the AUC0–24 hin 2-monthold rats was 140.91719.47 ng h/m L,which is 54.64% higher than that in 9-month-old *** AUC0–24 h ratio between H-Nim and L-Nim was 2.44 in 2-month-old rats and 3.06 in 9-month-old *** age difference could result in unparallelled change of the absorption and bioavailability of the polymorphic drugs,the results in this experiment are of value for further investigation of crystal form selection in clinical trials and rational clinical application of the polymorphic drugs.

关键词： Nimodipine Age difference Polymorphic drug Pharmacokinetics Crystal form

UHPLC-LTQ-Orbitrap-based metabolomics coupled with metabolomics pathway analysis method for exploring the protection mechanism of Kudiezi injection in a rat anti-ischemic cerebral reperfusion damage model

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Natural Medicines 2017年第12期15卷 955-960页

作者： LIU Si-Yi CAI Wei WANG Fei LIU Ying SHANG Zhan-Peng ZHANG Xiu-Ping WANG Zi-Jian LU Jian-Qiu ZHANG Jia-Yu Beijing research Institution of Chinese Medicine Beijing University of Chinese Medicine dong pharmaceutical research of Hunan Key Laboratory Hunan University of Medicine

Kudiezi injection has been used extensively in the treatment of cerebrovascular and cardiovascular diseases. However, its therapeutic effects and underlying mechanism of action are not fully understood. The aim of the present study was to clarify the protective mechanisms of Kudiezi injection on cerebral ischemic injury, using metabolomics methods. Middle cerebral artery occlusion(MCAO) was introduced in rats to build the cerebral ischemic damage. UHPLC-LTQ-Orbitrap-based analytical method was established for analysis of the metabolites. The raw mass data of all samples were normalized with Sieve 2.2 software and then introduced to orthogonal partial least squares discriminant analysis(OPLS-DA) model. Finally, 23 metabolites in plasma(15 were tentatively identified) were chosen as potential biomarkers, according to accurate mass measurements(< 5 ppm), MS/MS fragmentation patterns, and diagnostic product ions. Furthermore, on the basis of metabolic pathway analysis via metabolomics pathway analysis(Met PA), we first discovered that the protection mechanism in anti-ischemic cerebral reperfusion damage of Kudiezi injection was possibly related to the biosynthesis of phenylalanine, tyrosine, and tryptophan. The present study provided a useful approach for exploring the mechanism of ischemic stroke and evaluating the efficacy of Kudiezi injection or other traditional medicines.

关键词： Metabolomics Kudiezi injection Metabolomics pathway analysis(Met PA) Middle cerebral artery occlusion(MCAO) UHPLC-LTQ-Orbitrap