检索结果-南通市图书馆

The Innovation 2024年第1期5卷 88-97页

作者： Yijian Zhang Duo Li Yang Liu Liuqi Peng Dongdong Lu Pinpin Wang Dongxu Ke Huilin Yang Xuesong Zhu Changshun Ruan Department of Orthopaedics The First Affiliated Hospital of Soochow UniversitySoochow UniversitySuzhou 215006China research center for human Tissue and Organ degeneration Institute of Biomedicine and BiotechnologyShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China University of Chinese Academy of Sciences Beijing 100049China Novaprint Therapeutics Suzhou Co. Ltd.Room 605B1 BuildingBioBayNo.218 Xinghu StreetSuzhou Industrial ParkSuzhou 215000China

Reconstruction of osteochondral(OC)defects represents an immense challenge due to the need for synchronous regeneration of special stratified tissues.The revolutionary innovation of bioprinting provides a robust method for precise fabrication of tissue-engineered OCs with hierarchical structure;however,their spatial living cues for simultaneous fulfilment of osteogenesis and chondrogenesis to reconstruct the cartilage-bone interface of OC are underappreciated.Here,inspired by natural OC bilayer features,anisotropic bicellular living hydrogels(ABLHs)simultaneously embedding articular cartilage progenitor cells(ACPCs)and bone mesenchymal stem cells(BMSCs)in stratified layers were precisely fabricated via two-channel extrusion bioprinting.The optimum formulation of the 7%GelMA/3%AlgMA hydrogel bioink was demonstrated,with excellent printability at room temperature and maintained high cell viability.Moreover,the chondrogenic ability of ACPCs and the osteogenic ability of BMSCs were demonstrated in vitro,confirming the inherent differential spatial regulation of ABLHs.

关键词： printing stratified boost

Dual stimulus responsive borosilicate glass(BSG)scaffolds promote diabetic alveolar bone defectsrepair by modulating macrophage phenotype

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Bioactive Materials 2023年第8期26卷 231-248页

作者： Pengfei Tian Limin Zhao Jua Kim Xian Li Chunyu Liu Xu Cui Tao Liang Yunbo Du Xiehui Chen Haobo Pan center for human tissues and organs degeneration Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen518055PR China Shenzhen Longhua District Central Hospital Shenzhen518000PR China Shenzhen Healthemes Biotechnology Co.Ltd Shenzhen518102PR China

The regeneration of alveolar bone is still clinical challenge,particularly accompanied with diabetes,causing metabolic disorder with a protracted low-grade inflammatory phenotype.As a result,the anticipated loading of biomaterials is highly suspicious in spontaneous modulation of cells function,which is mostly disturbed by constant inflammation.In this study,we developed glucose and hydrogen peroxide dual-responsive borosilicate glass(BSG)scaffolds loaded with epigallocatechin gallate(EGCG)to synergistically modulate the abnormal inflammation of diabetic alveolar bone defects.It was found that the release of EGCG by BSG could directly regulate the shift of macrophages from M1 to the M2 phenotype by promoting autophagy and lessening the inhibition of autophagic flux.Moreover,EGCG can also indirectly regulate the polarization phenotype of macrophages by reducing the activation of NF-κb in stem cells and restoring its immunoregulatory capacity.Therefore,the addition of EGCG to BSG scaffold in diabetes allows for a more striking modulation of the macrophage phenotype in a timely manner.The altered macrophage phenotype reduces local inflammation and thus increases the ability to repair diabetic alveolar bone,showing promise for the treatment of alveolar defect in diabetic patients.

关键词： Borosilicate Epigallocatechin gallate(EGCG) Alveolar bone Autophagy Mitochondria Macrophage immunomodulatory Diabetes

Transdermal treatment for malignant melanoma by aptamer-modified tetrahedral framework nucleic acid delivery of vemurafenib

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2024年第4期35卷 260-267页

作者： Dexuan Xiao Tianyu Chen Tianxu Zhang Sirong Shi Mei Zhang Xin Qin Yunkun Liu Longjiang Li Yunfeng Lin State Key Laboratory of Oral Diseases National Clinical Research Center for Oral DiseasesWest China Hospital of StomatologySichuan UniversityChengdu 610041China Sichuan Provincial Engineering research center of Oral Biomaterials Chengdu 610041China Department of Head and Neck Oncology National Clinical Research Center for Oral DiseasesWest China Hospital of StomatologySichuan UniversityChengdu 610041China

Melanoma is one of the most malignant skin tumors, whose high invasion is generally associated with BRAF gene mutation. Although new chemotherapeutic drugs, such as vemurafenib, have been developed to inhibit the growth of melanoma, these drugs are usually administered intravenously or orally, resulting in toxic side effects on major tissues and organs. Tetrahedral framework nucleic acids(tFNAs) are a novel type of DNA nanostructures with excellent biocompatibility and versatility which have been proven to penetrate through skin barrier with ease. In this study, we prepared t FNAs with vemurafenib and connected DNA aptamer AS1411 at the apex of t FNAs(AS1411-tFNAs/vemurafenib). On one hand, AS1411-tFNAs/vemurafenib could kill melanoma cells by blocking the mutated BRAF gene in vitro. Compared with free vemurafenib, AS1411-tFNAs/vemurafenib had no obvious toxicity to normal cells. On the other hand,AS1411-tFNAs could transfer vemurafenib to cross through the skin barrier and permeate into tumor tissues. In vivo, transdermal delivery of AS1411-t FNAs/vemurafenib could inhibit the growth of human A375melanoma, whose inhibiting effect was stronger than intravenous administration of vemurafenib. These results demonstrated the application prospects of tFNAs combined with chemotherapeutic drugs in skin tumors.

关键词： Melanoma Vemurafenib DNA nanostructure Transdermal treatment Side effect

Nanomaterial-assisted theranosis of bone diseases

在线全文

学校读者我要写书评

暂无评论

Bioactive Materials 2023年第6期24卷 263-312页

作者： Kai Zheng Jiaxiang Bai Huilin Yang Yaozeng Xu Guoqing Pan Huaiyu Wang Dechun Geng Department of Orthopedics The First Affiliated Hospital of Soochow University188 Shizi RoadSuzhou215006JiangsuChina center for human tissues and organs degeneration Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen518055China Institute for Advanced Materials School of Materials Science and EngineeringJiangsu UniversityZhenjiang212013JiangsuChina

Bone-related diseases refer to a group of skeletal disorders that are characterized by bone and cartilage destruction.Conventional approaches can regulate bone homeostasis to a certain extent.However,these therapies are still associated with some undesirable problems.Fortunately,recent advances in nanomaterials have provided unprecedented opportunities for diagnosis and therapy of bone-related diseases.This review provides a comprehensive and up-to-date overview of current advanced theranostic nanomaterials in bone-related diseases.First,the potential utility of nanomaterials for biological imaging and biomarker detection is illustrated.Second,nanomaterials serve as therapeutic delivery platforms with special functions for bone homeostasis regulation and cellular modulation are highlighted.Finally,perspectives in this field are offered,including current key bottlenecks and future directions,which may be helpful for exploiting nanomaterials with novel properties and unique functions.This review will provide scientific guidance to enhance the development of advanced nanomaterials for the diagnosis and therapy of bone-related diseases.

关键词： Nanomaterials Delivery Diagnosis Therapy Bone-related diseases

Expansion of human umbilical cord derived mesenchymal stem cells in regenerative medicine

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Stem Cells 2024年第4期16卷 410-433页

作者： Shafiqa Naeem Rajput Bushra Kiran Naeem Anwar Ali Asmat Salim Irfan Khan Dr.Panjwani center for Molecular Medicine and Drug research International Center for Chemical and Biological SciencesUniversity of KarachiKarachi 75270SindhPakistan Surgical Unit 4 Dr.Ruth KM Pfau Civil HospitalKarachi 74400Pakistan Department of Physiology University of KarachiKarachi 75270Pakistan center for Regenerative Medicine and Stem Cells research and Department of Ophthalmology and Visual SciencesThe Aga Khan UniversityKarachi 74800SindhPakistan

BACKGROUND Stem cells are undifferentiated cells that possess the potential for self-renewal with the capacity to differentiate into multiple lineages.In humans,their limited numbers pose a challenge in fulfilling the necessary demands for the regeneration and repair of damaged tissues or organs.Studies suggested that mesenchymal stem cells(MSCs),necessary for repair and regeneration via transplantation,require doses ranging from 10 to 400 million cells.Furthermore,the limited expansion of MSCs restricts their therapeutic application.AIM To optimize a novel protocol to achieve qualitative and quantitative expansion of MSCs to reach the targeted number of cells for cellular transplantation and minimize the limitations in stem cell therapy protocols.METHODS human umbilical cord(hUC)tissue derived MSCs were obtained and re-cultured.These cultured cells were subjected to the following evaluation pro-cedures:Immunophenotyping,immunocytochemical staining,trilineage differentiation,population doubling time and number,gene expression markers for proliferation,cell cycle progression,senescence-associatedβ-galactosidase assay,human telomerase reverse transcriptase(hTERT)expression,mycoplasma,cytomegalovirus and endotoxin detection.RESULTS Analysis of pluripotent gene markers Oct4,Sox2,and Nanog in recultured hUC-MSC revealed no significant differences.The immunophenotypic markers CD90,CD73,CD105,CD44,vimentin,CD29,Stro-1,and Lin28 were positively expressed by these recultured expanded MSCs,and were found negative for CD34,CD11b,CD19,CD45,and HLA-DR.The recultured hUC-MSC population continued to expand through passage 15.Proliferative gene expression of Pax6,BMP2,and TGFb1 showed no significant variation between recultured hUC-MSC groups.Nevertheless,a significant increase(P<0.001)in the mitotic phase of the cell cycle was observed in recultured hUC-MSCs.Cellular senescence markers(hTERT expression andβ-galactosidase activity)did not show any negative effect on recultured hUC-MSCs.Additionally,quality control assessments co

关键词： human umbilical cord Mesenchymal stem cells Expansion Cell proliferation In vitro expansion Senescence

Hot deformation behavior of Cu-bearing antibacterial titanium alloy

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Materials Science & Technology 2018年第10期34卷 1867-1875页

作者： Zheng Ma Ling Ren M.Babar Shahzad Rui Liu Ying Zhao Ke Yang Institute of Metal research Chinese Academy of Sciences center for human tissues and organs degeneration Shenzhen Institute of Advanced Technology Chinese Academy of Science

We investigated the deformation behavior of a new biomedical Cu-bearing titanium alloy （Ti-645 （Ti-6.06AI-3.75V-4.85Cu, in wt%）） to optimize its microstructure control and the hot-working process. The results showed that true stress-true strain curve ofti-645 alloy was susceptible to both deformation tem-perature and strain rate. The microstructure of Ti-645 alloy was significantly changed from equiaxed grain to acicular one with the deformation temperature while a notable decrease in grain size was recorded as well. Dynamic recovery （DRV） and dynamic recrystallization （DRX） obviously existed during the thermal compression of Ti-645 alloy. The apparent activation energies in （α ＋ β） phase and β single phase regions were calculated to be 495.21 kJ mo1^-1 and 195.69 kJ mo1^-1, respectively. The processing map showed that the alloy had a large hot-working region whereas the optimum window occurred in the strain rate range of 0.001-0.1 s-1, and temperature range of 900-960℃ and 1000-1050℃. The obtained results could provide a technological basis for the design of hot working procedure of Ti-645 alloy to optimize the material design and widen the ootential application of Ti-645 alloy in clinic.

关键词： cu-bearing titanium alloy Constitutive equation Thermal deformation Microstructure Processing map

Injectable self-healing hydrogel with siRNA delivery property for sustained STING silencing and enhanced therapy of intervertebral disc degeneration

在线全文

学校读者我要写书评

暂无评论

Bioactive Materials 2022年第3期7卷 29-43页

作者： Jiaxin Chen Haifeng Zhu Yutao Zhu Chenchen Zhao Shengyu Wang Yixin Zheng Ziang Xie Yang Jin Honghai Song Linjun Yang Jin Zhang Jiayong Dai Zhijun Hu Huaiyu Wang Department of Orthopaedic Surgery Sir Run Run Shaw HospitalZhejiang University School of MedicineKey Laboratory of Musculoskeletal System DegenerationRegeneration Translational Research of Zhejiang ProvinceEast Qing Chun RoadHangzhou310016PR China College of Chemical Engineering Fuzhou University2 Xueyuan RoadFuzhou350108PR China center for human tissues and organs degeneration Shenzhen Institutes of Advanced TechnologyChinese Academy of SciencesShenzhen518055GuangdongPR China

Inflammatory responses of nucleus pulposus(NP)can induce imbalanced anabolism and catabolism of extracellular matrix,and the cytosolic dsDNA accumulation and STING-NF-κB pathway activation found in NP inflammation are considered as fairly important cause of intervertebral disc(IVD)degeneration.Herein,we constructed a siSTING delivery hydrogel of aldehyde hyaluronic acid(HA-CHO)and poly(amidoamine)PAMAM/siRNA complex to intervene the abnormal STING signal for IVD degeneration treatment,where the formation of dynamic Schiff base bonds in the system(siSTING@HPgel)was able to overcome the shortcomings such as low cellular uptake,short half-life,and rapid degradation of siRNA-based strategy.PAMAM not only formed complexes with siRNA to promote siRNA transfection,but also served as dynamic crosslinker to construct hydrogel,and the injectable and self-healing hydrogel efficiently and steadily silenced STING expression in NP cells.Finally,the siSTING@HPgel significantly eased IVD inflammation and slowed IVD degeneration by prolonging STING knockdown in puncture-induced IVD degeneration rat model,revealing that STING pathway was a therapeutic target for IVD degeneration and such novel hydrogel had great potential for being applied to many other diseases for gene delivery.

关键词： STING IVD degeneration siRNA delivery Injectable hydrogel Dynamic Schiff base

Roles of focal adhesion proteins in skeleton and diseases

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2023年第3期13卷 998-1013页

作者： Sheng Chen Tailin He Yiming Zhong Mingjue Chen Qing Yao Di Chen Zengwu Shao Guozhi Xiao Department of Orthopaedics Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Department of Biochemistry School of MedicineShenzhen Key Laboratory of Cell MicroenvironmentGuangdong Provincial Key Laboratory of Cell Microenvironment and Disease ResearchSouthern University of Science and TechnologyShenzhen 518055China research center for human tissues and organs degeneration Shenzhen Institutes of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China

The skeletal system,which contains bones,joints,tendons,ligaments and other elements,plays a wide variety of roles in body shaping,support and movement,protection of internal organs,production of blood cells and regulation of calcium and phosphate metabolism.The prevalence of skeletal diseases and disorders,such as osteoporosis and bone fracture,osteoarthritis,rheumatoid arthritis,and intervertebral disc degeneration,increases with age,causing pain and loss of mobility and creating a huge social and economic burden globally.Focal adhesions(FAs)are macromolecular assemblies that are composed of the extracellular matrix(ECM),integrins,intracellular cytoskeleton and other proteins,including kindlin,talin,vinculin,paxillin,pinch,Src,focal adhesion kinase(FAK)and integrin-linked protein kinase(ILK)and other proteins.FA acts as a mechanical linkage connecting the ECM and cytoskeleton and plays a key role in mediating cell–environment communications and modulates important processes,such as cell attachment,spreading,migration,differentiation and mechanotransduction,in different cells in skeletal system by impacting distinct outside-in and inside-out signaling pathways.This review aims to integrate the up-to-date knowledge of the roles of FA proteins in the health and disease of skeletal system and focuses on the specific molecular mechanisms and underlying therapeutic targets for skeletal diseases.

关键词： Focal adhesion Skeleton Mechanotransduction Signal transduction Integrin Cartilage Intervertebral disc Skeletal diseases

Novel strategies for the treatment of osteoarthritis based on biomaterials and critical molecular signaling

在线全文

学校读者我要写书评

暂无评论

Journal of Materials Science & Technology 2023年第18期149卷 42-55页

作者： Xingyun Huang Zhiyong Wang Huaiyu Wang Di Chen Liping Tong research center for Computer-aided Drug Discovery Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China Faculty of Pharmaceutical Sciences Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China University of Chinese Academy of Sciences Beijing 100049China center for human tissues and organs degeneration Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China

Osteoarthritis(OA)is a degenerative disease involving entire joint.It is often initiated from the low-grade inflammation in synovial tissue and then affects articular cartilage and subchondral bone.Multiple risk factors,such as aging,mechanical overloading,trauma,overuse,etc.are involved in OA development.Several approaches have been utilized to repair cartilage defects.Among them,biomaterials-based mes-enchymal stromal cell(MSCs)therapy is considered as the most promising modality.The burgeoning material science and manufacturing technologies,such as 3D printing,allow us to mimic native articu-lar cartilage and regulate the artificial cartilage development,regeneration and functional restoration.In this review article,we will summarize the recent progress of biomaterials combined with MSCs or chon-drocytes in repairing cartilage damage induced by OA.Several typical natural and synthetic biomaterials,such as collagen,alginate,hyaluronic acid and poly(ethylene glycol),polylactide acid,polyurethane,etc.for cartilage repairing will be introduced.Moreover,critical signaling pathways associated with the pro-gression of OA,as well as the targeted pharmacologic,genetic therapies and tissue engineering scaffolds for OA and cartilage repairing are presented.We will also provide our prospects for future directions in this active research area.

关键词： Osteoarthritis Cartilage Repairing Biomaterials 3D printing Signaling pathways

Nociceptive behavioural assessments in mouse models of temporomandibular joint disorders

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Journal of Oral Science 2020年第3期12卷 241-248页

作者： Jun Li Kaige Ma Dan Yi Chun-do Oh Di Chen Department of Orthopedic Surgery Rush University Medical CenterChicagoIL 60612USA research center for human tissues and organs degeneration Shenzhen Institutes of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China

Orofacial pain or tenderness is a primary symptom associated with temporomandibular joint(TMJ)disorders(TMDs).To understand the pathological mechanisms underlying TMDs,several mouse models have been developed,including mechanical stimulus-induced TMD and genetic mouse models.However,a lack of feasible approaches for assessing TMD-related nociceptive behaviours in the orofacial region of mice has hindered the in-depth study of TMD-associated mechanisms.This study aimed to explore modifications of three existing methods to analyse nociceptive behaviours using two TMD mouse models:(1)mechanical allodynia was tested using von Frey filaments in the mouse TMJ region by placing mice in specially designed chambers;(2)bite force was measured using the Economical Load and Force(ELF)system;and(3)spontaneous feeding behaviour tests,including eating duration and frequency,were analysed using the Laboratory Animal Behaviour Observation Registration and Analysis System(LABORAS).We successfully assessed changes in nociceptive behaviours in two TMD mouse models,a unilateral anterior crossbite(UAC)-induced TMD mouse model and aβ-catenin conditional activation mouse model.We found that the UAC model andβ-catenin conditional activation mouse model were significantly associated with signs of increased mechanical allodynia,lower bite force,and decreased spontaneous feeding behaviour,indicating manifestations of TMD.These behavioural changes were consistent with the cartilage degradation phenotype observed in these mouse models.Our studies have shown reliable methods to analyse nociceptive behaviours in mice and may indicate that these methods are valid to assess signs of TMD in mice.

关键词： disorders behaviour signs