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Transcriptome-Wide Identification and Functional Analysis of PgSQE08-01 Gene in Ginsenoside Biosynthesis in Panax ginseng ***.

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Phyton-International Journal of Experimental Botany 2024年第2期93卷 313-327页

作者： Lei Zhu Lihe Hou Yu Zhang Yang Jiang Yi Wang Meiping Zhang Mingzhu Zhao Kangyu Wang College of Life Science Jilin Agricultural UniversityChangchun130118China Jilin Engineering research center ginseng genetic resources development and utilization Changchun130118China Jilin Academy of Vegetable and Flower Sciences Changchun130033China

Panax ginseng *** an important plant species used in traditional Chinese medicine,whose primary active ingredient is a *** biosynthesis is not only regulated by transcription factors but also controlled by a variety of structural ***,the molecular mechanism underlying ginsenoside biosynthesis has always been a topic in the discussion of ginseng secondary *** epoxidase(SQE)is a key enzyme in the mevalonic acid pathway,which affects the biosynthesis of secondary metabolites such as *** ginseng transcriptome,expression,and ginsenoside content databases,this study employed bioinformatic methods to systematically analyze the genes encoding SQE in *** first selected six PgSQE candidates that were closely involved in ginsenoside biosynthesis and then identified PgSQE08-01 to be highly associated with ginsenoside ***,we constructed the overexpression vector pCAMBIA3301-PgSQE08-01 and the RNAi vector pART27-PgSQE08-01 and transformed ginseng adventitious roots using Agrobacterium rhizogenes,to obtain positive hairy-root ***,quantitative reverse transcriptionpolymerase chain reaction and high-performance liquid chromatography were used to determine the expression of relevant genes and ginsenoside content,***,we focused on the function of PgSQE08-01 gene,which was noted to be involved in ginsenoside ***,these findings not only provided a molecular basis for the identification of important functional genes in ginseng but also enriched genetic resources for the biosynthesis of ginsenosides using synthetic biology.

关键词： Panax ginseng pgSQE08-01 gene squalene epoxidase ginsenoside ginseng hairy roots

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Response of the ginseng C_(2)H_(2)-Type Zinc Finger Protein Family PgZFPs Gene to Methyl Jasmonate Regulation

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Phyton-International Journal of Experimental Botany 2024年第11期93卷 3055-3071页

作者： Yue Jiang Lingyu Liu Kangyu Wang Mingzhu Zhao Ping Chen Jun Lei Yanfang Wang Meiping Zhang Yi Wang Guang Chen College of Life Science Jilin Agricultural UniversityChangchun130118China research center for ginseng genetic resources development and utilization Jilin ProvinceJilin Agricultural UniversityChangchun130118China College of Chinese Medicinal Materials Jilin Agricultural UniversityChangchun130118China

The main active components of ginseng are ginsenosides,which play signiﬁcant roles in treating cardiovascular diseases,cancer,and providing antioxidant *** are primarily synthesized through the mevalo-nate pathway and the methylerythritol phosphate *** key enzyme genes involved in this biosynthetic process have been cloned and validated,yet the regulatory functions of transcription factors remain *** C_(2)H_(2)-type zincﬁnger protein family,one of the largest families of transcription factors,is crucial in plant growth and development,response to biotic and abiotic stresses,and regulation of secondary *** study,based on the ginseng transcriptome database from Jilin,conducted a correlation analysis between the expression levels of PgZFPs genes in the Jilin ginseng C_(2)H_(2)-type zincﬁnger protein family and ginsenoside content,a gen-ome-wide association study of PgZFPs,and co-expression analysis of PgZFPs with validated key enzyme ***,ﬁve candidate genes involved in ginsenoside biosynthesis were identiﬁ*** involvement of PgZFP27 and PgZFP-59-02 genes from the PgZFPs family in the biosynthesis of ginsenosides was validated through in vitro methyl jasmonate(MeJA)induction *** result provides new genetic resources for the biosynthesis of ginsenosides.

关键词： Ginsenosides C_(2)H_(2)-type zincﬁnger protein family MeJA induction functional study SIT SITIENS FLC FLACCA MVA Mevalonate MEP 2-C-methyl-D-erythritol 4-phosphate GWAS Genome-wide association studies HPLC High-performance liquid chromatography

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Identification of candidate piRNAs in the gonads of Paralichthys olivaceus (Japanese flounder)

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Zoological research 2016年第5期37卷 301-306页

作者： Chun-Lei WANG Zhi-Peng WANG Jia-Qi WANG Ming-You Li Xiao-Wu CHEN Key Laboratory of Exploration and utilization of Aquatic genetic resources Ministry of EducationShanghai Ocean UniversityShanghai 201306China

Piwi-interacting RNA （piRNA） plays an important role in the gonadal development and maintenance of Teleostei. In this study, piRNA libraries derived from the adult gonads of Japanese flounder （Paralichthys ofivaceus） were generated using next-generation sequencing technology. Using zebrafish piRNAs as a reference, 5 865 unique candidate piRNAs were identified; 289 candidate piRNA clusters （PRCs） were generated from the above piRNAs. Among the isolated candidate PRCs, a total of 38 ovary-specific, 45 ovary-bias, 24 testis-specific, and 131 testis-bias PRCs were found. The relative expression levels of seven PRCs were validated through quantitative reverse transcription-polymerase chain reaction. The results of this study will help facilitate exploration of the development and maintenance of the phenotypic sex mechanism in P. olivaceus.

关键词： Paralichthys olivaceus Ovary Testis piRNA

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development of SRAP Markers Linked to a Gene for Stem Nematode Resistance in Sweetpotato, Ipomoea batatas (L.) Lam.

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Journal of Integrative Agriculture 2013年第3期12卷 414-419页

作者： ZHAO Ning ZHAI Hong YU Xiao-xia LIU Zhe-sheng HE Shao-zhen LI Qiang MA Dai-fu LIU Qing-chang Beijing Key Laboratory of Crop genetic Improvement/Laboratory of Crop Heterosis and utilization Ministry of Education/China Agricultural University Xuzhou Sweetpotato research center

Sequence-related amplification polymorphism （SRAP） markers closely linked to stem nematode resistance gene were developed in sweetpotato, lpomoea batatas （L.） Lam. Using bulked segregant analysis （BSA）, 200 SRAP primer combinations were screened with the resistant and susceptible bulked DNA from the 196 progenies of an F1 single-cross population of resistant parent Xu 781xsusceptible parent Xushu 18, 77 of them showed polymorphic bands between resistant and susceptible DNA. Primer combinations detecting polymorphism between the two bulks were used to screen both parents and 10 individuals from each of the bulks. The results showed that primer combination A9B4 produced 3 specific bands in the resistant plants but not in the susceptible plants, suggesting that the markers, named Nspl, Nsp2 and Nsp3, respectively, linked to a gene for stem nematode resistance. Primer combination A3B6 also produced a SRAP marker named Nsp4 linking to the resistance gene. Amplified analysis of the 196 F1 individuals indicated that the genetic distance between these markers and the resistance gene was 4.7, 4.7, 6.3, and 9.6 cM, respectively.

关键词： Ipomoea batatas (L.) Lam. SRAP marker stem nematode

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Identification of QTLs for Starch Content in Sweetpotato(Ipomoea batatas(L.) Lam.)

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Journal of Integrative Agriculture 2014年第2期13卷 310-315页

作者： YU Xiao-xia ZHAO Ning LI Hui JIE Qin ZHAI Hong HE Shao-zhen LI Qiang LIU Qing-chang Laboratory of Crop Heterosis and utilization Ministry of Education/Beijing Key Laboratory of Crop Genetic Improvement/China Agricultural University Xuzhou Sweetpotato research center

Sweetpotato （Ipomoea batatas （L.） Lam.） breeding is challenging due to its genetic complexity. In the present study, interval mapping （IM） and multiple quantitative trait locus （QTL） model （MQM） analysis were used to identify QTLs for starch content with a mapping population consisting of 202 F1 individuals of a cross between Xushu 18, a cultivar susceptible to stem nematodes, with high yield and moderate starch, and Xu 781, which is resistant to stem nematodes, has low yield and high starch content. Six QTLs for starch content were mapped on six linkage groups of the Xu 781 map, explaining 9.1-38.8% of the variation. Especially, one of them, DMFN 4, accounted for 38.8% of starch content variation, which is the QTL that explains the highest phenotypic variation detected to date in sweetpotato. All of the six QTLs had a positive effect on the variation of the starch content, which indicated the inheritance derived from the parent Xu 781. Two QTLs for starch content were detected on two linkage groups of the Xushu 18 map, explaining 14.3 and 16.1% of the variation, respectively. They had a negative effect on the variation, indicating the inheritance derived from Xu 781. Seven of eight QTLs were co-localized with a single marker. This is the first report on the development of QTLs co-localized with a single marker in sweetpotato. These QTLs and their co-localized markers may be used in marker-assisted breeding for the starch content of sweetpotato.

关键词： co-localized marker lpomoea batatas (L.) Lam. QTLs starch content

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bmp10 maintains cardiac function by regulating iron homeostasis

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Journal of genetics and Genomics 2024年第12期51卷 1459-1473页

作者： Ruiqin Hu Genfang Li Peng Hu Hongbo Niu Wenhao Li Shouwen Jiang Guijun Guan Qianghua Xu Mingli Liu Liangbiao Chen International research center for Marine Bioscience (Ministry of Science and Technology) Shanghai Ocean University Key Laboratory of Exploration and utilization of Aquatic genetic resources (Ministry of Education) Shanghai Ocean University Key Laboratory of Sustainable Exploitation of Oceanic Fisheries resources College of Marine Science Shanghai Ocean University

Heart disease remains the leading cause of death worldwide. Iron imbalance, whether deficiency or overload, contributes to heart failure. However, the molecular mechanisms governing iron homeostasis in the heart are poorly understood. Here, we demonstrate that mutation of bmp10, a heart-born morphogen crucial for embryonic heart development, results in severe anemia and cardiac hypertrophy in ***, bmp10 deficiency causes cardiac iron deficiency, which later progresses to iron overload due to the dysregulated hepcidin/ferroportin axis in cardiac cells, leading to ferroptosis and heart failure. Early iron supplementation in bmp10-/-mutants rescues erythropoiesis, while iron chelation in juvenile fishes significantly alleviates cardiac hypertrophy. We further demonstrate that the interplay between HIF1adriven hypoxic signaling and the IL6/p-STAT3 inflammatory pathways is critical for regulating cardiac iron metabolism. Our findings reveal BMP10 as a key regulator of iron homeostasis in the vertebrate heart and highlight the potential of targeting the BMP10-hepcidin-iron axis as a therapeutic strategy for iron-related cardiomyopathy.

关键词： Anemia Cardiac hypertrophy Ferroptosis Inflammation Iron bmp10

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Toxic effects of nano-TiO_(2)in bivalves——A synthesis of meta-analysis and bibliometric analysis

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Journal of Environmental Sciences 2021年第6期33卷 188-203页

作者： Zhuoqing Li Menghong Hu Hanting Song Daohui Lin Youji Wang International research center for Marine Biosciences at Shanghai Ocean University Ministry of Science and TechnologyShanghai 201306China Key Laboratory of Exploration and utilization of Aquatic genetic resources Ministry of EducationShanghai Ocean UniversityShanghai 201306China Department of Environmental Science Zhejiang UniversityHangzhou 310058China

Since the beginning of the 21 st century,the increasing production and application of nano-TiO_(2)in consumer products have inevitably led to its release into aquatic systems and therefore caused the exposure of aquatic organisms,resulting in growing environmental ***,the safety of nano-TiO_(2)in aquatic environments has not been systematically assessed,especially in coastal and estuary waters where a large number of filterfeeding animals *** are considered around the world to be a unique target group for nanoparticle toxicity,and numerous studies have been conducted to test the toxic effects of nano-TiO_(2)on *** aim of this review was to systematically summarize and analyze published data concerning the toxicological effects of nano-TiO_(2)in *** particular,the toxicity of nano-TiO_(2)to the antioxidant system and cell physiology was subjected to meta-analysis to reveal the mechanism of the toxicological effects of nano-TiO_(2)and the factors affecting its toxicological *** reveal the cooperation,hot keywords and cocitations in this field,bibliometric analysis was conducted,and the results showed that the toxicological molecular mechanisms of nano-TiO_(2)and the combined effects of nano-TiO_(2)and other environmental factors are two major hot ***,some perspectives and insights were provided in this review for future research on nano-TiO_(2)toxicology in bivalves.

关键词： Meta-analysis Titanium dioxide nanoparticles Toxic effect Bivalves Bibliometric analysis

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Targeted mutations of BnPAP2 lead to a yellow seed coat in Brassica napus L.

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Journal of Integrative Agriculture 2024年第2期23卷 724-730页

作者： Wei Huang Ruyu Jiao Hongtao Cheng Shengli Cai Jia Liu Qiong Hu Lili Liu Bao Li Tonghua Wang Mei Li Dawei Zhang Mingli Yan Hunan research center of Heterosis utilization in Rapeseed/Crop research Institute Hunan Academy of Agricultural SciencesChangsha 410125China Hunan Key Laboratory of Economic Crops genetic Improvement and Integrated utilization/School of Life and Health Science Hunan University of Science and TechnologyXiangtan 411201China Key Laboratory of Biology and genetic Improvement of Oil Crops Ministry of Agriculture and Rural Affairs/Oil Crops Research InstituteChinese Academy of Agricultural SciencesWuhan 430062China

The yellow seed trait is preferred by breeders for its potential to improve the seed quality and commercial value of Brassica *** the present study,we produced yellow seed mutants using a CRISPR/Cas9 system when the two BnPAP2 homologs were knocked *** staining of the seed coat demonstrated that proanthocyanidin accumulation was significantly reduced in the pap2 double mutants and decreased specifically in the endothelial and palisade layer cells of the seed *** and metabolite profiling analysis suggested that disruption of the BnPAP2 genes could reduce the expression of structural and regulated genes in the phenylpropanoid and flavonoid biosynthetic *** broad suppression of these genes might hinder proanthocyanidin accumulation during seed development,and thereby causing the yellow seed trait in *** results indicate that BnPAP2 might play a vital role in the regulatory network controlling proanthocyanidin accumulation.

关键词： yellow seed BnPAP2 proanthocyanidins CRISPR/Cas9

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Single somatic cell development and differentiation in the gametiphytic blades of Pyropia suborbiculata (Bangiales, Rhodophyta)

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Aquaculture and Fisheries 2021年第5期6卷 479-484页

作者： Wenxia Guo Hongchang Ding Xinghong Yan Key Laboratory of Exploration and utilization of Aquatic genetic resources Ministry of EducationShanghai Ocean UniversityShanghai201306China Shanghai Engineering research center of Aquaculture Shanghai Ocean UniversityShanghai201306China

Conchospores released from a wild-type strain of Pyropia suborbiculata were cultured in the laboratory for 20-80 *** blades of different ages were enzymatically isolated to obtain single cells,which then developed into different types of regenerative plants within a liquid *** regenerative plant types includes:normal blades,abnormal blades,cell-masses,and sexual *** age of mother blades distinctly affected the number and proportion of each type of regenerative *** the age of mother blades increased from 20 to 80 days,the proportion of normal blades in the isolated cells sharply decreased,the percentage of abnormal blades increased,and the proportion of cell-masses was relatively *** 40 days of cultivation,sexual cell-masses appeared in regenerated plants derived from the single blade cells of the PS-WT strain,and gradually increased with mother cell *** addition,the proportion of normal blades decreased in regenerated plants sourced from the basal to apical parts of the same alga,while the proportion of abnormal blades and cell-masses *** summary,the isolated cells of the gametophytic blade of *** developed into different types of regenerated plantlets in vivo due to the different stages of cell *** preliminarily concluded that the differentiation from conchospores to the sexual cells could be divided into at least seven different groups.

关键词： Pyropia suborbiculata development Differentiation Single somatic cells Regenerative plants

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Single or combined effects of dietary arabinoxylan-oligosaccharide and inulin on growth performance,gut microbiota,and immune response in Pacific white shrimp Litopenaeus vannamei

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Journal of Oceanology and Limnology 2021年第2期39卷 741-754页

作者： Yun LI Wei YUAN Yi ZHANG Hong LIU Xilin DAI International research center for Marine Biosciences at Shanghai Ocean University Ministry of Science and TechnologyShanghai 201306China Key Laboratory of Exploration and utilization of Aquatic genetic resources(Shanghai Ocean University) Ministry of EducationShanghai 201306China National Demonstration center for Experimental Fisheries Science Education(Shanghai Ocean University) Shanghai 201306China

The single and synergistic effects of dietary arabinoxylan-oligosaccharide(AXOS)and inulin at different doses(2,4,and 8 mg/g diet)on survival,growth performance,gut microbiota,and immune response in Pacific white shrimp Litopenaeus vannamei were *** application of either inulin or AXOS at doses of 4 mg/g diet showed the most stimulatory effects on the growth rate and gene expression levels of chitinase,cathepsin L,chymotrypsin,and extracellular signal-regulated kinase(ERK)in each single prebiotic feeding *** with single prebiotic treatments,simultaneous application of AXOS and inulin at 4 mg/g diet enhanced remarkably the growth parameters of shrimp and the expression of related genes(chitinase,cathepsin L,chymotrypsin,ERK,myeloid differentiation factor 88,and phenoloxidase)after 8-week feeding(P<0.05).Additionally,gut microbiota analysis indicated the dietary supplementation with combined prebiotics increased significantly the bacterial community richness and relative abundance of Bacillus Pseudomonas Bacteriovorax,and Lactobacillus,and reduced the abundance of Vibrio Rhodococcus,and Photobacterium in the digestive tract of L *** with the single prebiotic treatment and the control,combined prebiotics supplementation boosted notably the survival rate and expression levels of immune-related genes in shrimp infected with Vibrio alginolyticus or white spot syndrome ***,simultaneous application of AXOS and inulin shall have a great potential of dietary supplement in the culture of ***.

关键词： prebiotic growth intestinal microflora immunity survival Litopenaeus vannamei

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