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Experimental research on a Feasible Building Insulation Material Based Reed in China

Journal of Thermal Science 2025年第1期 176-187页

作者： LIU Tiantian PENG Yizhe LIU Lifang CAI Chenghan WANG Shuang LI Hongqiang College of Civil Engineering Hunan University College of Civil Engineering Hunan University of Science and Technology School of Energy and Power Jiangsu University of Science and Technology National center for International research collaboration in Building Safety and Environment (NCIRCBSE) Hunan University Key Laboratory of Building Safety and Energy Efficiency of the Ministry of Education Hunan University

The seemingly useless reeds are prepared as thermal insulation materials,which not only meet the requirements of environmental sustainability but also enhance the added value of reeds,creating new economic *** hydrophobicity of raw biomass surfaces leads to problems such as weak bonding strength and non-dense structure in the formed materials,as well as issues related to the residual insect infestations on the *** this study,reed straw was used as the raw material,and foamed geopolymer was used as the binder to prepare building insulation materials based *** improve the interfacial adhesion performance between reed straw and foamed geopolymer,a thermochemical modification method-thermal carbonization,was *** this study,the mechanical properties and hydraulic properties of the studied materials with different degrees of surface thermal modification were tested,especially the fire resistance performance,and weathering resistance performance rarely found in published *** the surface thermal modification condition of reed straw was 250℃（30 min）,the comprehensive performance of reed-based building insulation materials was the best,when the studied material density was 321.3 kg/m3;the compressive strength was 0.59 MPa;the thermal conductivity was 0.101 W/（m·K）;the pH was 11.27;the moisture absorption rate was 25.1%,and the compressive strength loss rate in wet-dry cycles was 18.5%.In addition,it had excellent fire resistance performance and weathering resistance *** new material can be widely used to improve the thermal insulation of traditional buildings and as sandwich filler in prefabricated buildings,such as preparing insulating walls.

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Adaptation in polymerase basic protein 1 family from influenza A virus to climate change

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Health 2012年第10期4卷 968-973页

作者： Shaomin Yan Guang Wu State Key Laboratory of Non-Food biomass Enzyme Technology National Engineering Research Center for Non-Food Biorefinery Guangxi Key Laboratory of Biorefinery Guangxi Academy of Sciences Nanning China

Global climate changes affect the functioning of ecosystems, in particular host-pathogen interactions, with major consequences in health ecology, however, it is less addressed how the change in global temperature affects the protein family of influenza virus. In this study, we studied the adaptation of polymerase basic protein 1 (PB1) family from influenza A virus to temperature change. 3841 PB1 proteins sampled from 1956-2011 were quantified by the amino-acid pair predictability and then compared their general changes with the temperature changes (Had-CRUT3v and CRUTEM4v data sets) of corresponding years on a 5? by 5? grid-box basis. Also, point-to-point comparisons were conducted from 1956 to 1998 in all and different species. The results showed that both changes in the temperature and unpredictable portion of PB1 proteins had similar trends from 1956 to 2011, which provides the evidence of virus adaptation at protein level to climate change.

关键词： Adaptation Evolution Influenza A Virus Polymerase Basic Protein 1 Temperature Change

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2-Dimensional HP Foldings of Dermaseptin-J2

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Engineering（科研） 2013年第10期5卷 78-84页

Although the hydrophobic-polar (HP) model is a simple model to study protein folding, it is an approximation to the real-life case. Dermaseptin is a subfamily of frog skin active peptide family, which has various antimicrobial activities, and dermaseptin-J2 is a newly found peptide composed of 26 amino acids. In this study, the 2-dimensional HP model was used to analyze the foldings of dermaseptin-J2 and its nine mutants, which were converted to different HP sequences according to the normalized amino acid hydrophobicity index with respect to pH levels and the conversion of glycine as hydrophobic or polar, and each has 847,288,609,443 possible foldings. The results show that the foldings with minimal energy have different native states, which are chiral and can be numerically distinguished and ranked according to the normalized amino acid hydrophobicity index. The nine mutants of dermaseptin-J2 do not affect the minimal energy but affect their native states at pH 7. The results demonstrate that two pH levels and conversion of glycine as hydrophobic or polar affect the native state and minimal energy, suggesting these are two ways to modify dermaseptin-J2.

关键词： Dermaseptin Folding Configuration HP Model Hydrophobicity Index Minimal Energy Native State

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Elaboration of Simplest Folding Structures in 2-Dimensional Lattice with Delta-Hemolysin and Its Variants in HP Model

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Journal of Biomedical Science and Engineering 2019年第5期12卷 293-309页

作者： Shaomin Yan Guang Wu State Key Laboratory of Non-food biomass Enzyme Technology National Engineering Research Center for Non-Food Biorefinery Guangxi Key Laboratory of Biorefinery Guangxi Academy of Sciences Nanning China

Although the advanced 3-dimensional structure measurements provide more and more detailed structures in Protein Data Bank, the simplest 2-dimensional lattice model still looks meaningful because 2-dimensional structures play a complementary role with respect to 3-dimensional structures. In this study, the folding structures of delta-hemolysin and its six variants were studied at 2-dimensional lattice, and their amino acid contacts in folding structures were considered according to HP model with the aid of normalized amino acid hydrophobicity index. The results showed that: 1) either delta-hemolysin or each of its variants could find any of its folding structure in one eighth of 1,129,718,145,924 folding structures because of symmetry, which reduces the time required for folding, 2) the impact of pH on folding structures is varying and associated directly with the amino acid sequence itself, 3) the changes in folding structures of variants appeared different case by case, and 4) the assigning of hydrophobicity index to each amino acid was a way to distinguish folding structures at the same native state. This study can help to understand the structure of delta-hemolysin, and such an analysis can shed lights on NP-problem listed in millennium prize because the HP folding in lattice belongs to a sub-problem of NP-problem.

关键词： Folding Structure Delta-Hemolysin HP Model Hydrophobicity Index Lattice Minimal Energy Native State

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Safety assessment of a novel marine multi-stress-tolerant yeast Meyerozyma guilliermondii GXDK6 according to phenotype and whole genome-sequencing analysis

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Food Science and Human Wellness 2024年第4期13卷 2048-2059页

作者： Xueyan Mo Mengcheng Zhou Yanmei Li Lili Yu Huashang Bai Peihong Shen Xing Zhou Haojun Zhu Huijie Sun Ru Bu Chengjian Jiang State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources Guangxi Research Center for Microbial and Enzyme Engineering TechnologyCollege of Life Science and TechnologyGuangxi UniversityNanning 530004China National Engineering research center for Non-Food biorefinery State Key Laboratory of Non-Food Biomass and Enzyme TechnologyGuangxi Research Center for Biological Science and TechnologyGuangxi Academy of SciencesNanning 530007China Guangxi Key Laboratory for Green Processing of Sugar Resources College of Biological and Chemical EngineeringGuangxi University of Science and TechnologyLiuzhou 545006China

The application of microorganisms as probiotics is limited due to lack of safety ***,a novel multi-stress-tolerant yeast Meyerozyma guilliermondii GXDK6 with aroma-producing properties was identified from marine mangrove *** safety and probiotic properties were assessed in accordance with phenotype and whole-genome sequencing *** showed that the genes and phenotypic expression of related virulence,antibiotic resistance and retroelement were rarely *** morphogenesis genes(SIT4,HOG1,SPA2,ERK1,ICL1,CST20,HSP104,TPS1,and RHO1)and phospholipase secretion gene(VPS4)were *** hyphae and phospholipase were *** one retroelement(Tad1-65_BG)was *** biogenic amines(BAs)encoding genes were absent,except for spermidine synthase(JA9_002594),spermine synthase(JA9_004690),and tyrosine decarboxylase(inx).The production of single BAs and total BAs was far below the food-defined ***6 had no resistance to common antifungal *** enzymes,such as gelatinase,DNase,hemolytic,lecithinase,and thrombin were *** toxicity test with mice demonstrated that GXDK6 is ***6 has a good reproduction ability in the simulation gastrointestinal ***6 also has a strong antioxidant ability,β-glucosidase,and inulinase *** sum up,GXDK6 is considered as a safe probiotic for human consumption and food fermentation.

关键词： Meyerozyma guilliermondii Safety assessment Probiotics Marine mangrove microorganisms Whole-genome sequencing analysis

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Correlating Combined Features of Amino Acid and Protein with Crystallization Propensity of Proteins from <i>Mycobacterium tuberculosis</i>

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Journal of Biomedical Science and Engineering 2019年第9期12卷 427-442页

作者： Shaomin Yan Guang Wu National Engineering research center for Non-Food biorefinery State Key Laboratory of Non-Food Biomass and Enzyme Technology Guangxi Key Laboratory of Bio-Refinery Guangxi Biomass Engineering Technology Research Center Guangxi Academy of Sciences Nanning China

Since a decade ago, both protein and amino acid features have been correlated with crystallization propensity of proteins in order to develop methods to predict whether a protein can be crystallized. In this continuing study, each of three features combining features of amino acid and protein, was correlated with the crystallization propensity of proteins from Mycobacterium tuberculosis using logistic and neural network models. The results showed that two combined features, amino acid distribution probability and future composition, had good predictions on whether a protein would be crystallized in comparison with the predictions obtained from each of 531 amino acid features. The results obtained from the third combined feature, amino acid pair predictability, demonstrated the trend of crystallization propensity in proteins from Mycobacterium tuberculosis.

关键词： Protein Feature Mycobacterium tuberculosis Protein Crystallization

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Predictors for Predicting Temperature Optimum in Beta-Glucosidases

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Journal of Biomedical Science and Engineering 2019年第8期12卷 414-426页

作者： Shaomin Yan Guang Wu State Key Laboratory of Non-Food biomass and Enzyme Technology Guangxi Key Laboratory of Bio-Refinery Guangxi Biomass Engineering Technology Research Center National Engineering Research Center for Non-Food Biorefinery Guangxi Academy of Sciences Nanning China

This is the continuation of our studies on beta-glucosidase, which plays an important role in biological processes and recently strong interests focus on their potential role in biofeul production. In order to develop simple methods to predict the optimal working condition for beta-glucosidase, we used a 20-1 feedforward backpropagation neural network to screen possible predictors to predict the temperature optimum of beta-glucosidase from 25 amino-acid properties related to the primary structure of beta-glucosidases. The results show that the normalized polarizability index and amino-acid distribution probability can predict the temperature optimum of beta-glucosidase, which highlights a cost-effective way to predict various enzymatic parameters of beta-glucosidase.

关键词： Beta-Glucosidase Enzyme Temperature Optimum Prediction

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Predicting pH Optimum for Activity of Beta-Glucosidases

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Journal of Biomedical Science and Engineering 2019年第7期12卷 354-367页

作者： Shaomin Yan Guang Wu State Key Laboratory of Non-Food biomass and Enzyme Technology Guangxi Key Laboratory of Bio-Refinery National Engineering Research Center for Non-Food Biorefinery Guangxi Biomass Engineering Technology Research Center Guangxi Academy of Sciences Nanning China

The working conditions for enzymatic reaction are elegant, but not many optimal conditions are documented in literatures. For newly mutated and newly found enzymes, the optimal working conditions can only be extrapolated from our previous experience. Therefore a question raised here is whether we can use the knowledge on enzyme structure to predict the optimal working conditions. Although working conditions for enzymes can be easily measured in experiments, the predictions of working conditions for enzymes are still important because they can minimize the experimental cost and time. In this study, we develop a 20-1 feedforward backpropagation neural network with information on amino acid sequence to predict the pH optimum for the activity of beta-glucosidase, because this enzyme has drawn much attention for its role in bio-fuel industries. Among 25 features of amino acids being screened, the results show that 11 features can be used as predictors in this model and the amino-acid distribution probability is the best in predicting the pH optimum for the activity of beta-glucosidases. Our study paves the way for predicting the optimal working conditions of enzymes based on the amino-acid features.

关键词： Beta-Glucosidase Enzyme pH Optimum Prediction

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Do We Ignore Tobacco’s Positive Ecological Role Too Long?

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Natural Science 2020年第5期12卷 273-280页

Definitely, tobacco is one of the most troubling plants in this planet because of its harmful effects on humans. Therefore, tobacco plantation declines continuously in the world. For such a plant, do we need to eliminate tobacco entirely from the surface of the earth? Perhaps, humans might have ignored the tobacco’s positive role in environment and ecology, especially in the heavily industrialized environments, for too long. Because the human activity generates more and more nitrogen oxides (NOx) in atmosphere, which not only cause imbalance in the global nitrogen cycle but also lead to haze, smog, acid rain, PM2.5, and eventually impact on environment and human health. Unfortunately the current technologies do not provide an efficient way to remove NOx from atmosphere. However, it is only tobacco can remove NOx from atmosphere. Perhaps, we should blame us, humans who use tobacco unwisely, rather than blame tobacco in nature. Anyways, the ability of tobacco to remove NO2 from atmosphere should not be ignored.

关键词： Air Pollution Nitrogen Oxides PM2.5 Phytoremediation Tobacco

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2,5-Diformylfuran production by photocatalytic selective oxidation of 5-hydroxymethylfurfural in water using MoS_(2)/CdIn_(2)S_(4) flower-like heterojunctions

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Chinese Journal of Chemical Engineering 2023年第2期54卷 180-191页

作者： Qian Zhu Yan Zhuang Hongqing Zhao Peng Zhan Cong Ren Changsheng Su Wenqiang Ren Jiawen Zhang Di Cai Peiyong Qin National Energy R&D center for biorefinery Beijing University of Chemical TechnologyBeijing 100029China Collage of Life Science and Technology Beijing University of Chemical TechnologyBeijing 100029China research center for Eco-environmental Sciences Chinese Academy of ScienceBeijing 100085China

The selective oxidation of 5-hydroxymethylfurfural(HMF) into 2,5-diformylfuran(DFF) is an important reaction for renewable biomass building blocks. Compared with thermal catalytic processes, photocatalytic production of DFF from HMF has attracted tremendous attention. Herein, the MoS_(2)/CdIn_(2)S_(4)(MC)flower-like heterojunctions were prepared and considered as photocatalysts for selective oxidation of HMF into DFF under visible-light irradiation in aqueous solution. Results demonstrated MoS_(2) in MC heterojunction could promote the separation of photoexcited electron-hole pairs, while the amount of MoS_(2) dropping was proved influenced on the photocatalytic performance. 80.93% of DFF selectivity was realized when using 12.5% MC as photocatalyst. In addition, the MC catalyst also showed great potential in transformation of other biomass derived benzyl-and furyl-alcohols. The catalytic mechanism suggested that ·O_(2)^(-) was the decisive active radical for HMF oxidation. Therefore, the MC heterojunction could be applied in photocatalytic conversion of biomass to valuable chemicals under ambient condition.

关键词： 2 5-Diformylfuran(DFF) Photocatalysis MoS_(2)/CdIn_(2)S_(4)(MC) Selective oxidation Visible light irradiation

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