检索结果-南通市图书馆

Free or fixed state of nHAP differentially regulates hBMSC morphology and osteogenesis through the valve role of ITGA7

引用

Bioactive Materials 2022年第12期7卷 539-551页

作者： Fangyuan Bao Junzhi Yi Yixiao Liu Yuliang Zhong Hui Zhang Zhonglin Wu Boon Chin Heng Ying Wang Ziyang Wang Lizi Xiao Hua Liu Hongwei Ouyang Jing Zhou dr Li dak Sum&Yip Yio Chin center for Stem Cells and regenerative medicine and Department of Orthopedic Surgery of The Second Affiliated HospitalZhejiang University School of MedicineHangzhouPR China department of Sports medicine Zhejiang University School of MedicineHangzhouPR China Zhejiang University-University of Edinburgh Institute Zhejiang University School of Medicineand Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang ProvinceZhejiang University School of MedicineHangzhouPR China China Orthopedic regenerative medicine Group(COrMed) HangzhouPR China School of Stomatology Peking UniversityBeijingPR China Zhongshan School of medicine Sun Yat-sen UniversityGuangzhouPR China

Nano-hydroxyapatite(nHAP)has been widely used in bone repair as an osteo-inductive and naturally-occurring ***,the optimal applied form of nHAP and the underlying mechanisms involved remain ***,to investigate into these,a range of corresponding models were designed,including three applied forms of nHAP(Free,Coating and 3d)that belong to two states(Free or fixed).The results indicate that when fixed nHAP was applied in the 3d form,optimal osteogenesis was induced in human bone marrow stem cells(hBMSCs)with increased bone volume via integrinα7(ITGA7)-mediated upregulation of the PI3K-AKT signaling pathway,while contrary results were observed with free *** osteogenesis experiments in mice subcutaneous transplantation model further confirmed the different tendencies of ITGA7 expression and osteogenesis of hBMSCs in free and fixed states of *** results revealed that the two states of nHAP play a different regulatory role in cell morphology and osteogenesis through the valve role of ITGA7,providing cues for better application of nanoparticles and a potential new molecular target in bone tissue engineering.

关键词： Applied forms nHAP Osteogenesis ITGA7

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Tissue-Engineered Products for skin regenerative medicine

引用

Open Journal of regenerative medicine 2016年第3期5卷 61-84页

作者： Yoshimitsu Kuroyanagi r & d center skin regenerative medicine Technosurg Ltd. Sagamihara Japan

In a general wound healing process, foreign bodies and tissue detritus have to be broken down and then a new tissue is produced. However, the new tissue formation sometimes fails to proceed under the impaired conditions such as burn injury and intractable skin ulcer. A major obstruction to wound healing is infection. Another obstruction to wound healing is deficiency of growth factors. The endogenous levels of growth factors are reduced in some chronic wounds. To improve these wound conditions, researchers have been trying to create several types of artificial skins. The tissue-engineered products include three prime constituents, i.e., cells, growth factors, and materials. In this review, the practical design of tissue-engineered products for skin regenerative medicine is introduced. The first design makes it possible to release silver sulfadiazine (AgSd) from a wound dressing. The second design makes it possible to release Epidermal Growth Factor (EGF) from a wound dressing or a skin care product composed of hyaluronic acid spongy sheet containing bioactive ingredients. The third design makes it possible to release several types of growth factors from allogeneic fibroblasts within cultured dermal substitute. This tissue-engineered product is prepared by seeding allogeneic fibroblasts into a collagen and hyaluronic acid spongy sheet. Although allogeneic cells are rejected gradually in immune system, they are able to release some types of growth factors, thereby regenerating a damaged tissue. The clinical study demonstrates that these tissue-engineered products are promising for the treatment of burn injury and intractable skin ulcer.

关键词： Tissue Engineering Wound dressing Cultured skin Substitute Antimicrobial Agent Growth Factor

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

dynamic photoelectrical regulation of ECM protein and cellular behaviors

引用

Bioactive Materials 2023年第4期22卷 168-179页

作者： Xiaozhao Wang Cai Yao Xudong Yao Junxin Lin rui Li Kun Huang Weiming Lin Xiaojun Long Chao dai Jiajun dong Xuegong Yu a Wenwen Huang Wenjian Weng Qi Wang Hongwei Ouyang Kui Cheng School of Materials Science and Engineering State Key Laboratory of Silicon MaterialsCyrus Tang Center for Sensor Materials and ApplicationsZhejiang UniversityHangzhou310027China dr.Li dak Sum&Yip Yio Chin center for Stem Cells and regenerative medicine Department of Orthopedic Surgery of the Second Affiliated HospitalZhejiang University School of MedicineHangzhou310058China department of Sports medicine Zhejiang University School of MedicineHangzhou310058China Zhejiang University-University of Edinburgh Institute Zhejiang University School of MedicineKey Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang ProvinceZhejiang University School of MedicineHangzhou314400China China Orthopedic regenerative medicine Group(COrMed) Hangzhou310058China School of Chemistry Zhejiang UniversityHangzhou310027China International Institutes of medicine The Fourth Affiliated HospitalZhejiang University School of MedicineNo.N1Shangcheng AvenueYiwu322000China

dynamic regulation of cell-extracellular matrix(ECM)-material interactions is crucial for various biomedical *** this study,a light-activated molecular switch for the modulation of cell attachment/detachment behaviors was established on monolayer graphene(Gr)/n-type Silicon substrates(Gr/Si).Initiated by light illumination at the Gr/Si interface,pre-adsorbed proteins(bovine serum albumin,ECM proteins collagen-1,and fibronectin)underwent protonation to achieve negative charge transfer to Gr films(n-doping)throughπ-π*** n-doping process stimulated the conformational switches of ECM *** structural alterations in these ECM interactors significantly reduced the specificity of the cell surface receptor-ligand interaction(e.g.,integrin recognition),leading to dynamic regulation of cell adhesion and eventual cell ***-sequencing results revealed that the detached bone marrow mesenchymal stromal cell sheets from the Gr/Si system manifested regulated immunoregulatory properties and enhanced osteogenic differentiation,implying their potential application in bone tissue *** work not only provides a fast and feasible method for controllable cells/cell sheets harvesting but also gives new insights into the understanding of cell-ECM-material communications.

关键词： Graphene Protein conformational change Charge transfer Cell-materials interaction n-doping

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A long non-coding rNA LncSync regulates mouse cardiomyocyte homeostasis and cardiac hypertrophy through coordination of mirNA actions

引用

Protein & Cell 2023年第2期14卷 153-157页

作者： rujin Huang Jinyang Liu Xi Chen Ying Zhi Shuangyuan ding Jia Ming Yulin Li Yangming Wang Jie Na center for Stem Cell Biology and regenerative medicine School of MedicineTsinghua UniversityBeijing 100084China School of Life Sciences Tsinghua UniversityBeijing 100084China Capital Medical University Beijing 100084China Beijing Key Laboratory of Cardiometabolic Molecular medicine Peking-Tsinghua Center for Life SciencesInstitute of Molecular MedicinePeking UniversityBeijing 100871China

dear Editor,Maintenance of cardiomyocyte(CM)homeostasis is essential for normal heart ***-term imbalance in heart homeostasis could elicit irreversible adaptive change in cell structure and function and tissue architecture,exemplified as cardiac hyper-trophy and fibrosis,and eventually develop into heart failure(Shiojima et al.,2005).Therefore,identifying new genes and path-ways regulating CM homeostasis may help better understand the cause of cardiac hypertrophy.

关键词： homeostasis cardiac hypertrophy

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Progress of Proximity Sensors for Potential Applications in Electronic skins

引用

Transactions of Tianjin University 2024年第1期30卷 40-62页

作者： runnan Zou Yanhong Tong Jiayi Liu Jing Sun da Xian Qingxin Tang center for Advanced Optoelectronic Functional Materials research Key Lab of UV-Emitting Materials and Technology of Ministry of EducationNortheast Normal UniversityChangchun 130024China

recently,electronic skins and fl exible wearable devices have been developed for widespread applications in medical monitoring,artifi cial intelligence,human–machine interaction,and artifi cial *** proximity sensors can accurately perceive external objects without contact,introducing a new way to achieve an ultrasensitive perception of *** article reviews the progress of fl exible capacitive proximity sensors,fl exible triboelectric proximity sensors,and fl exible gate-enhanced proximity sensors,focusing on their applications in the electronic skin fi ***,their working mechanism,materials,preparation methods,and research progress are discussed in ***,we summarize the future challenges in developing fl exible proximity sensors.

关键词： Capacitive proximity sensors Triboelectric proximity sensors Gate-enhanced proximity sensors Flexible wearable devices Electronic skins

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A high-resolution route map reveals distinct stages of chondrocyte dedifferentiation for cartilage regeneration

引用

Bone research 2022年第3期10卷 578-593页

作者： Yishan Chen Yeke Yu Ya Wen Juan Chen Junxin Lin Zixuan Sheng Wenyan Zhou Heng Sun Chengrui An Jiansong Chen Weiliang Wu Chong Teng Wei Wei Hongwei Ouyang dr.Li dak Sum&Yip Yio Chin center for Stem Cells and regenerative medicine and Department of Orthopedic Surgery of the Second Affiliated HospitalZhejiang University School of MedicineHangzhou 310058China department of Sports medicine Zhejiang University School of MedicineHangzhou 310058China Zhejiang University-University of Edinburgh Institute Zhejiang University School of Medicineand Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang ProvinceZhejiang University School of MedicineHangzhou 310058China department of Oral Surgery Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghai 200011China department of General Intensive Care Unit The Second Affiliated Hospital of Zhejiang University School of MedicineHangzhou 310009China department of Orthopedic Surgery The Children’s HospitalZhejiang University School of MedicineNational Clinical Research Center for Child HealthHangzhou 310052China department of Orthopedic Surgery The Fourth Affiliated HospitalZhejiang University School of MedicineYiwu 322000China China Orthopedic regenerative medicine Group(COrMed) Hangzhou 310058China

Articular cartilage damage is a universal health *** recent progress,chondrocyte dedifferentiation has severely compromised the clinical outcomes of cell-based cartilage ***-of-function changes are frequently observed in chondrocyte expansion and other pathological conditions,but the characteristics and intermediate molecular mechanisms remain *** this study,we demonstrate a time-lapse atlas of chondrocyte dedifferentiation to provide molecular details and informative biomarkers associated with clinical chondrocyte *** performed various assays,such as single-cell rNA sequencing(scrNA-seq),live-cell metabolic assays,and assays for transposase-accessible chromatin with high-throughput sequencing(ATAC-seq),to develop a biphasic dedifferentiation model consisting of early and late dedifferentiation ***-stage chondrocytes exhibited a glycolytic phenotype with increased expression of genes involved in metabolism and antioxidation,whereas late-stage chondrocytes exhibited ultrastructural changes involving mitochondrial damage and stress-associated chromatin *** the chemical inhibitor BTB06584,we revealed that early and late dedifferentiated chondrocytes possessed distinct recovery potentials from functional phenotype ***,this two-stage transition was also validated in human *** image-based approach was established for clinical use to efficiently predict chondrocyte plasticity using stage-specific ***,this study lays a foundation to improve the quality of chondrocytes in clinical use and provides deep insights into chondrocyte dedifferentiation.

关键词： chondrocyte cartilage metabolism

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Targeted inhibition of osteoclastogenesis reveals the pathogenesis and therapeutics of bone loss under sympathetic neurostress

引用

International Journal of Oral Science 2022年第4期14卷 479-488页

作者： Bingdong Sui Jin Liu Chenxi Zheng Lei dang Ji Chen Yuan Cao Kaichao Zhang Lu Liu Minyan dang Liqiang Zhang Nan Chen Tao He Kun Xuan Fang Jin Ge Zhang Yan Jin Chenghu Hu State Key Laboratory of Military Stomatology and National Clinical research center for Oral diseases and Shaanxi International Joint research center for Oral diseases Center for Tissue EngineeringSchool of StomatologyThe Fourth Military Medical UniversityXi'anChina Law Sau Fai Institute for Advancing Translational medicine in Bone and Joint diseases School of Chinese MedicineHong Kong Baptist UniversityHong Kong SARChina Xi'an Key Laboratory of Stem Cell and regenerative medicine Institute of Medical ResearchNorthwestern Polytechnical UniversityXianChina Xi'an Institute of Tssue Engineering and regenerative medicine XianChina department of Biochemistry and Molecular Biology School of Basic Medical SciencesXi'an Jiaotong UniversityXi'anChina

Sympathetic cues via the adrenergic signaling critically regulate bone homeostasis and contribute to neurostress-induced bone loss,but the mechanisms and therapeutics remain incompletely ***,we reveal an osteoclastogenesis-centered functionally important osteopenic pathogenesis under sympatho-adrenergic activation with characterized micro rNA response and efficient *** discovered that osteoclastic mi r-21 was tightly regulated by sympatho-adrenergic cues downstream theβ2-adrenergic receptor(β2Ar)signaling,critically modulated osteoclastogenesis in vivo by inhibiting programmed cell death 4(Pdcd4),and mediated detrimental effects of both isoproterenol(ISO)and chronic variable stress(CVS)on ***,without affecting osteoblastic bone formation,bone protection against ISO and CVS was sufficiently achieved by a(d-Asp8)-lipid nanoparticle-mediated targeted inhibition of osteoclastic mi r-21 or by clinically relevant drugs to suppress ***,these results unravel a previously underdetermined molecular and functional paradigm that osteoclastogenesis crucially contributes to sympatho-adrenergic regulation of bone and establish multiple targeted therapeutic strategies to counteract osteopenias under stresses.

关键词： osteoclast pathogenesis homeostasis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Successful individualized endodontic treatment of severely curved root canals in a mandibular second molar:A case report

引用

World Journal of Clinical Cases 2022年第14期10卷 4632-4639页

作者： Lai-Jun Xu Jian-Ying Zhang Zi-Hua Huang Xiang-Zhu Wang Hunan Key Laboratory of Oral Health research Hunan 3D Printing Engineering Research Center of Oral CareHunan Clinical Research Center of Oral Major Diseases and Oral HealthAcademician Workstation for Oral-maxilofacial and Regenerative Medicine and Xiangya Stomatological HospitalXiangya School of StomatologyCentral South UniversityChangsha 410008Hunan ProvinceChina

BACKGrOUNd The incidence rate of severely curved root canals in mandibular molars is low,and the root canal treatment of mandibular molars with this aberrant canal anatomy may be technically *** SUMMArY A 26-year-old Chinese female patient presented with intermittent and occlusal pain in the left mandibular second *** patient had undergone filling restoration for caries before endodontic *** the aid of cone beam computed tomography(CBCT),a large periapical radiolucency was observed,and curved root canals in a mandibular second molar were confirmed,depicting a severe and curved distolingual *** treatments,including novel individualized preparation skills and techniques and the use of bioceramic materials as an apical barrier,were performed,and complete healing of the periapical lesion and a satisfactory effect were *** A case of severely curved root canals in a mandibular second molar was successfully treated and are reported *** complex anatomy of the tooth and the postoperative effect were also evaluated via the three-dimensional reconstruction of CBCT images,which accurately identified the aberrant canal *** devices and biomaterial applications combined with novel synthesis techniques can increase the success rate of intractable endodontic treatment.

关键词： Cone beam computed tomography Canal curvature Mandibular second molar root canal therapy Case report

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

design of Collagen-Based Hemostatic Material for Use in Plastic and reconstructive Surgery

引用

Open Journal of regenerative medicine 2022年第1期11卷 25-39页

作者： Yoshimitsu Kuroyanagi ryusuke Suzuki Misato Kuroyanagi r & d center for skin regenerative medicine Technosurg Ltd. Sagamihara Japan School of Allied Health Sciences Kitasato University Sagamihara Japan SHEM Inc. Tokyo Japan department of Emergency medicine Graduate School of Medicine Yokohama City University Yokohama Japan department of Plastic and reconstructive Surgery Graduate School of Medicine Yokohama City University Yokohama Japan

This study aims to develop collagen-based hemostatic materials. The sheet-shaped collagen sponge was manufactured by freeze-vacuum drying the aqueous solution of collagen (Col) and heat-denatured collagen (Col’) at a composition ratio of 2/1. The both sides or one side of sponge was treated with ultraviolet (UV) irradiation for 15 minutes to introduce intermolecular crosslinks between collagen molecules. The elution behavior of collagen sponge was investigated by immersing the sponge in water for a predetermined time and then by measuring the weight change. The double-sided UV-irradiated sponge showed very slow elution properties. On the other hand, the single-sided UV-irradiated sponge showed initially fast elution and subsequent very slow elution properties. Such initially fast elution of collagen molecules from the surface without UV-irradiation allows an adhesion of collagen sponge to the wound surface and results in hemostatic effect. In addition, the water absorption and retention properties of sponge were investigated by placing the hydrated sponge on a mesh for a predetermined time and then by measuring the weight change. The double-sided UV-irradiated sponge absorbed 81 times more water than own weight and showed a value of 45 times even after 7 days. The single-sided UV-irradiated sponge absorbed 80 times more water than own weight and showed a value of 39 times even after 7 days. The sponge with high water absorption and retention properties allows a wound healing effect because such sponge can absorb large amounts of blood plasma and exudates containing various cell growth factors. The double-sided UV-irradiated sponge is a good candidate for the wound dressing. On the other hand, the single-sided UV-irradiated sponge is a good candidate for the hemostatic material.

关键词： Sheet-Shaped Collagen Sponge Hemostatic Material Water Absorption and retention Property Hemostatic Effect Wound Healing Effect

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

design of Novel Wound dressing Composed of Collagen and Hyaluronic Acid Containing Epidermal Growth Factor

引用

Open Journal of regenerative medicine 2023年第2期12卷 49-63页

This research aims to develop a wound dressing composed of collagen (Col) and hyaluronic acid (HA) containing epidermal growth factor (EGF). First important issue is to contain EGF in the wound dressing in a stable state. The sheet-shaped sponge was manufactured by freeze-vacuum drying an aqueous solution of Col. Both sides of sponge were treated with ultraviolet (UV) irradiation to introduce intermolecular cross links between collagen molecules. This sponge was named Sponge-Col. Another sheet-shaped sponge was manufactured by freeze-vacuum drying an aqueous solution of HA containing EGF. This sponge was named Sponge-HA/EGF. The wound dressing was manufactured by laminating Sponge-Col on the top, Sponge-HA/EGF in the middle, and Sponge-Col on the bottom to create a sandwich structure. This method can prevent the reducing of EGF activity due to UV irradiation for intermolecular cross-linking. Second important issue is to enable gradual release of EGF from the wound dressing. The elution behavior of this wound dressing was investigated by measuring the weight change after immersion in water for a predetermined time. This wound dressing showed initially fast elution and subsequent very slow elution properties. The upper layer and lower layer Sponge-Col enabled gradual release of the middle layer Sponge-HA/EGF. This result suggests that EGF contained in the wound dressing is gradually released together with HA from the wound dressing. Third important issue is to provide moist wound-healing environment. The upper layer and lower layer Sponge-Col can provide the wound dressing with high water absorption and long-term water retention properties.

关键词： Collagen Hyaluronic Acid Epidermal Growth Factor Wound dressing

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：