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Surface engineering of carbon dots for highly sensitive α-glucosidase assay and inhibition evaluation

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Chinese Chemical Letters 2024年第3期35卷 193-197页

作者： Meijuan Liang Gege Song Yeqing Wan Yingying Chen Fuan Wang Xiaoqing Liu College of Chemistry and Molecular sciences Wuhan UniversityWuhan 430072China Oil Crops Research institute Chinese Academy of Agricultural SciencesWuhan 430062China

Monitoringα-glucosidase(α-Glu)activity is of great significance for the early diagnosis of typeⅡ*** the blue fluorescent carbon dots(CDs)were integrated with two different recognizing molecules,β-cyclodextrin and phenylboronic acid,for assembling a multifunctional CDs(mCDs)nanoplatform for sensitively analyzingα-Glu and its *** hydrolyzed product of 4-nitrophenyl-α-D-glucopyranoside(α-Glu substrate),p-nitrophenol,could efficiently quench the fluorescence of mCDs due to its cooperative molecular recognition withβ-cyclodextrin and phenylboronic *** mCDs could be utilized for the detection ofα-Glu activity with the limit of detection of 0.030 U/***,the presentα-Glu detection platform revealed a high selectivity,and other natural enzymes showed scarcely any effect on the present mCDs *** proposed method could be facilely used to screenα-Glu inhibitors with satisfying *** rational mCDs is expected to supplement more comprehensive biosensing platforms for highly sensitive and specific recognition of disease-relevant biomarkers with clinical importance.

关键词： α-Glucosidase Carbon dots Fluorescent sensor Inhibitor β-Cyclodextrin

Identification of an odorant receptor responding to sex pheromones in Spodoptera frugiperda extends the novel type-I PR lineage in moths

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Insect Science 2024年第2期31卷 489-502页

作者： Sai Zhang Emmanuelle Jacquin-Joly Nicolas Montagne Fang Liu Yang Liu Guirong Wang College of Horticulture and Plant Protection Yangzhou UniversityYangzhouChina State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBejingChina institute of Ecology and En vironmental sciences of Paris INRAESorbonne UniversityCNRSIRDUPECUniversity of ParisVersaillesFrance Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhenChina

moths,pheromone receptors(PRs)are crucial for intraspecific sexual communication between males and *** PRs are considered as an ideal model for studying the evolution of insect PRs,and a large number of PRs have been identified and functionally characterized in different moth *** PRs were initially thought to fall into a single monophyletic clade in the odorant receptor(OR)family,but recent studies have shown that ORs in another lineage also bind type-I sex pheromones,which indicates that type-I PRs have multiple independent origins in the *** this study,we investigated whether ORs of the pest moth Spodoptera frugiperda belonging to clades closely related to this novel PR lineage may also have the capacity to bind type-I pheromones and serve as male *** the 7 ORs tested,only 1(SfruOR23)exhibited a male-biased expression ***,in vitro functional characterization showed that SfruOR23 could bind several type-I sex pheromone compounds with Z-9-tetradecenal(Z9-14:Ald),a minor component found in female sex pheromone glands,as the optimal *** addition,SfruOR23 also showed weak responses to plant volatile organic ***,we characterized an *** PR positioned in a lineage closely related to the novel PR clade,indicating that the type-I PR lineage can be extended in moths.

关键词： odorant receptor pheromone receptor lineage sex pheromone Spodoptera frugiperda

Biochemistry and transcriptome analysis reveal condensed tannins alleviate liver injury induced by regulating cholesterol metabolism pathway

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Food Science and Human Wellness 2024年第2期13卷 909-919页

作者： Xiangxin Li Yijing Pu Bangdi Liu Xiaoming Fang Wenjun Peng Weibo Jiang Wenli Tian institute of Apicultural Research Chinese Academy of Agricultural SciencesBeijing 100093China College of Food Science and Nutritional Engineering China Agricultural UniversityBeijing 100083China Academy of agricultural Planning and Engineering Ministry of Agriculture and Rural AffairsBeijing 100125China

Free cholesterol has been considered to be a critical risk factor of nonalcoholic fatty liver disease(NAFLD).It remains unknown whether dietary intake of condensed tannins(CTs)have distinguishable effects to alleviate liver damage caused by a high cholesterol *** C57BL/6 mice were fed a high cholesterol diet for 6 weeks,and given CTs treatment at a dosage of 200 mg/(kg·day)at the same *** results indicated that compared with mice fed a normal diet,a high cholesterol diet group resulted in significant weight loss,dysregulation of lipid metabolism in blood and liver,and oxidative stress in the liver,but CTs treatment dramatically reversed these negative *** and eosin(H&E)staining and frozen section observation manifested that CTs treatment could effectively reduce the deposition of liver cholesterol and tissue necrosis caused by high cholesterol *** alleviated liver injury mainly by regulating the expression of related genes in cholesterol metabolism pathway and AMPK *** results confirmed that CTs have remarkable cholesterol lowering and anti-liver injury effects in vivo.

关键词： Condensed tannins High cholesterol Liver injury Antioxidants Transcriptomic analysis

Breeding maize of ideal plant architecture for high-density planting tolerance through modulating shade avoidance response and beyond

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Journal of Integrative Plant Biology 2024年第5期66卷 849-864页

作者： Fereshteh Jafari Baobao Wang Haiyang Wang Junjie Zou Biotechnology Research institute Chinese Academy of Agricultural SciencesBeijing 100081China Graduate School of Chinese Academy of agricultural sciences Beijing 100081China National Nanfan Research institute CAASSanya 572025China State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources College of Life SciencesSouth China AgriculturalUniversityGuangzhou 510642China

Maize is a major staple crop widely used as food,animal feed,and raw materials in industrial ***-density planting is a major factor contributing to the continuous increase of maize ***,high planting density usually triggers a shade avoidance response and causes increased plant height and ear height,resulting in lodging and yield *** plant height and ear height,more erect leaf angle,reduced tassel branch number,earlier flowering,and strong root system architecture are five key morphological traits required for maize adaption to high-density *** this review,we summarize recent advances in deciphering the genetic and molecular mechanisms of maize involved in response to high-density *** also discuss some strategies for breeding advanced maize cultivars with superior performance under high-density planting conditions.

关键词： flowering time high-density planting leaf angle maize plant architecture plant height/ear height root architecture tasselbranch

Patterns of crop-specific fertilizer-nitrogen losses and opportunities for sustainable mitigation:A quantitative overview of 15N-tracing studies

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Soil Ecology Letters 2024年第2期6卷 71-81页

作者： Cong Xu Hanshen Zhu Haokuang Liu Cheng Ji Jie Yuan Guanlin Li Jidong Wang Yongchun Zhang National agricultural Experimental Station for agricultural Environment LuheInstitute of Agricultural Resources and EnvironmentJiangsu Academy of Agricultural SciencesNanjing210014China School of the Environment and Safety Engineering Jiangsu UniversityZhenjiang212013China

Pattern and mitigation potential of crop-specific fertilizer-N losses were *** showed high fertilizer-N losses due to high N application rates and low ***,SOC,and soil pH are key parameters affecting fertilizer-N *** a given application rate,soils with higher SOC have lower fertilizer-N *** N rate combined with SOC improvement could cut 34.8%-59.6%of N losses.

关键词： fertilizer-nitrogen loss crop-specific 15N tracing soil organic carbon meta-analysis

Spatiotemporal evolution and assembly processes of ammonia-oxidizing prokaryotic communities in 1 000-year-old coastal reclaimed soils

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Pedosphere 2024年第04期34卷 709-725页

作者： Sarfraz HUSSAIN Yifan YIN Senlin LIU Shanshan YAN Dongjie CHEN Hui CAO Feng WANG Key Laboratory of agricultural Environmental Microbiology Ministry of Agriculture and Rural Affairs College of Life Sciences Nanjing Agricultural University institute of Eco-Environmental sciences Ningbo Academy of Agricultural Sciences

Coastal marshes are transitional areas between terrestrial and aquatic ecosystems. They are sensitive to climate change and anthropogenic activities. In recent decades, the reclamation of coastal marshes has greatly increased, and its effects on microbial communities in coastal marshes have been studied with great interest. Most of these studies have explained the short-term spatiotemporal variation in soil microbial community dynamics. However, the impact of reclamation on the community composition and assembly processes of functional microbes(e.g., ammonia-oxidizing prokaryotes) is often ignored. In this study, using quantitative polymerase chain reaction and the Ion S5? XL sequencing platform, we investigated the spatiotemporal dynamics, assembly processes, and diversity patterns of ammonia-oxidizing prokaryotes in 1 000-year-old reclaimed coastal salt marshes. The taxonomic and phylogenetic diversity and composition of ammonia oxidizers showed apparent spatiotemporal variations with soil reclamation. Phylogenetic null modelling-based analysis showed that across all sites, the archaeal ammonia-oxidizing community was assembled by a deterministic process(84.71%), and deterministic processes were also dominant(55.2%) for ammonia-oxidizing bacterial communities except for communities at 60 years of reclamation. The assembly process and nitrification activity in reclaimed soils were positively correlated. The abundance of the amoA gene and changes in ammonia-oxidizing archaeal and bacterial diversities significantly affected the nitrification activity in reclaimed soils. These findings suggest that long-term coastal salt marsh reclamation affects nitrification by modulating the activities of ammonia-oxidizing microorganisms and regulating their community structures and assembly processes. These results provide a better understanding of the effects of long-term land reclamation on soil nitrogen-cycling microbial communities.

关键词： ammonia oxidiser βNTI community assembly coastal salt marshes deterministic nitrification rate stochastic

Increased long-distance and homo-trans interactions related to H3K27me3 in Arabidopsis hybrids

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Journal of Integrative Plant Biology 2024年第2期66卷 208-227页

作者： Zhaoxu Gao Yanning Su Le Chang Guanzhong Jiao Yang Ou Mei Yang Chao Xu Pengtao Liu Zejia Wang Zewen Qi Wenwen Liu Linhua Sun Guangming He Xing Wang Deng Hang He School of Advanced Agriculture sciences and School of Life sciences State Key Laboratory of Protein and Plant Gene ResearchPeking UniversityBeijing 100871China College of Grassland Science Beijing Forestry UniversityBeijing 100083China Peking University institute of Advanced agricultural sciences Shandong Laboratory of Advanced Agricultural Sciences in WeifangWeifang 261325China

In plants,the genome structure of hybrids changes compared with their parents,but the effects of these changes in hybrids remain *** reciprocal crosses between Col×C24 and C24×Col in Arabidopsis using high-throughput chromosome conformation capture assay(Hi-C)analysis,we found that hybrid three-dimensional(3D)chromatin organization had more long-distance interactions relative to parents,and this was mainly located in promoter regions and enriched in genes with heterosis-related *** interactions between euchromatin and heterochromatin were increased,and the compartment strength decreased in *** compartment domain(CD)boundaries,the distal interactions were more in hybrids than their *** the hybrids of CURLY LEAF(clf)mutants clfCol×clfC24and clfC24×clfCol,the heterosis phenotype was damaged,and the long-distance interactions in hybrids were fewer than in their parents with lower ***-seq data revealed higher levels of H3K27me3 in the region adjacent to the CD boundary and the same interactional homo-trans sites in the wild-type(WT)hybrids,which may have led to more long-distance *** addition,the differentially expressed genes(DEGs)located in the boundaries of CDs and loop regions changed obviously in WT,and the functional enrichment for DEGs was different between WT and clf in the longdistance interactions and loop *** findings may therefore propose a new epigenetic explanation of heterosis in the Arabidopsis hybrids and provide new insights into crop breeding and yield increase.

关键词： 3D chromatin organization H3K27me3 homo-trans hybrids long-distanceinteractions

The auxin transporter OsAUX1 regulates tillering in rice(Oryza sativa)

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Journal of Integrative Agriculture 2024年第5期23卷 1454-1467页

作者： Luqi Jia Yongdong Dai Ziwei Peng Zhibo Cui Xuefei Zhang Yangyang Li Weijiang Tian Guanghua He Yun Li Xianchun Sang Chongqing Key Laboratory of Crop Molecular Improvement/Rice Research institute Academy of Agricultural SciencesSouthwest UniversityChongqing400715China Rice and Sorghum Research institute Sichuan Academy of Agricultural Sciences/Key Laboratory of Southwest Rice Biology and Genetic BreedingMinistry of Agriculture and Rural AffairsDeyang 618000China

Tillering is an important agronomic trait of rice(Oryza sativa)that affects the number of effective panicles,thereby affecting *** phytohormone auxin plays a key role in *** we identified the high tillering and semi-dwarf 1(htsd1)mutant with auxin-deficiency root characteristics,such as shortened lateral roots,reduced lateral root density,and enlarged root ***1 showed reduced sensitivity to auxin,but the external application of indole-3-acetic acid(IAA)inhibited its *** identified the mutated gene in htsd1 as AUXIN1(OsAUX1,LOC_Os01g63770),which encodes an auxin influx *** promoter sequence of OsAUX1 contains many SQUAMOSA PROMOTER BINDING PROTEIN-LIKE(SPL)binding sites,and we demonstrated that SPL7 binds to the OsAUX1 *** BRANCHED1(OsTB1),a key gene that negatively regulates tillering,was significantly downregulated in *** was enhanced in the OsTB1 knockout mutant,and the external application of IAA inhibited tiller elongation in this *** OsTB1 restored the multi-tiller phenotype of *** results suggest that SPL7 directly binds to the OsAUX1 promoter and regulates tillering in rice by altering OsTB1 expression to modulate auxin signaling.

关键词： rice(Oryza sativa L.) tillering indole-3-acetic acid(IAA) OsAUX1 OsTB1

Utilizing resequencing big data to facilitate Brassica vegetable breeding:tracing introgression pedigree and developing highly specific markers for clubroot resistance

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Horticultural Plant Journal 2024年第3期10卷 771-783页

作者： Zhiyong Ren Jinquan Li Xingyu Zhang Xingxu Li Junhong Zhang Zhibiao Ye Yuyang Zhang Qijun Nie Key Laboratory of Vegetable Ecological Cultivation on Highland Ministry of Agriculture and Rural AffairsHubei Key Laboratory of Vegetable Germplasm Enhancement and Genetic ImprovementInstitute of Economic CropsHubei Academy of Agricultural SciencesWuhanHubei 430063China National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops Huazhong Agricultural UniversityWuhanHubei 430070China Vegetable Research institute Wuhan Academy of Agricultural SciencesWuhanHubei 430345China Hubei Hongshan Laboratory WuhanHubei 430070China Shenzhen institute of Nutrition and Health Huazhong Agricultural UniversityWuhanHubei 430070China Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhenGuangdong 518000China

Clubroot caused by Plasmodiophora brassicae is a devastating disease of Cruciferous *** cultivars with clubroot resistance(CR)is the most effective control *** the two major Brassica vegetable species *** and ***,several commercial cultivars with unclear CR pedigrees have been intensively used as CR donors in ***,the continuous occurrence of CR-breaking makes the CR pedigree underlying these cultivars one of the breeders'most urgent *** complex intraspecific diversity of these two major Brassica vegetables has also limited the applicability of CR markers in different breeding *** we first traced the pedigree underlying two kinds of CR that have been widely applied in breeding by linkage and introgression analyses based on public resequencing *** ***,a major locus CRzi8 underlying the CR of the commercial CR donor‘DegaoCR117’was ***8 was further shown to have been introgressed from turnip(*** ***)and that it carried a potential functional allele of *** turnip introgression carried CRb^(c),sharing the same coding sequence with the CRb that was also identified from chromosome C07 of *** CR cultivars with different *** natural populations,variation analysis of linkage intervals of CRzi8,PbBa8.1,CRb,and CRb^(c)yielded easily resolved InDel markers(>20 bp)for these fundamental CR *** specificity of these markers was tested in diverse cultivars panels,and each exhibited high reliability in *** research demonstrates the value of the practice of applying resequencing big data to solve urgent concerns in breeding programs.

关键词： Brassica Clubroot resistance Resequencing Introgression analysis Molecular marker