检索结果-南通市图书馆

Journal of Donghua University(English Edition) 2006年第5期23卷 113-118页

作者：李峥嵘傅科杰孟卫东卿凤翎 College of Chemistry and Chemical Engineering Donghua University Textile products inspection Center of NingBo entry- exit inspection and quarantine bureau

A new class of novel polymeric crosslinging agent （NPCA）, which contained silane coupling group and the epoxy groups, was designed and synthesized in our laboratory. NPCA was a non-formaldehyde multifunctional crosslinking polymer. The cotton fabrics dyed with 8.0% （owf） Vinylsulpone-type dyes were treated with 3.0%- 4.0% NPCA, 0.5 mol/L potassium thiocyanate as a catalyst, then padded through two dips and two nips to reach a wet pickup of 80%- 85%, then dried at 80℃ for 2 minutes and cured in oven at 140- 150℃ for 3 minutes. Crocking fastness and fixation （%） were improved with up to 1.0 -1. 5 units, 35%- 50%, respectively. And there was little difference between the color yield of dyed fabrics before and after the treatment at certain conditions. The possible crosslinking mechanism of NPCA was also investigated. It was concluded that NPCA can improve colorfastness of cotton fabric by means of the three-dimensional network, covalent bonding and other molecular forces.

关键词： a crosslinking agent cotton fabric fastness property pad-dry-cure.

Rapid,sensitive detection of Vibrio anguillarum using loop-mediated isothermal amplification

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Oceanology and Limnology 2010年第1期28卷 62-66页

作者：高宏伟李富花张晓军王兵相建海 Institute of Oceanology Chinese Academy of Sciences Shandong entry-exit inspection and quarantine bureau of the People's Republic of China

Vibrio anguillarum is an important bacterial pathogen of aquatic organisms and a significant problem in aquatic farming. The rapid detection and identification of V. anguillarum, and other pathogens that infect marine organisms, is crucial to effective disease management. In this study, we developed a loop-mediated amplification （LAMP） assay to detect V. anguillarum in an hour in a single tube without the need for thermal cycling. Conserved regions of the metalloproteinase （empA） gene of V. anguillarum served as the targets for primer design. A fragment of the empA gene was amplified at 65℃ in the presence of the primer mixture and Bst DNA polymerase. In the optimized LAMP assay, 6.7 pg of V. anguillarum DNA could be detected. Six strains of V. anguillarum and 17 strains of non-V, anguillarum bacteria were used in this study to evaluate the species specificity of the primers. The six V. anguillarum strains gave a positive result in the LAMP assay. This method was also validated in V. anguillarum-infected fish. This LAMP method is more sensitive than PCR in the detection of V. anguillarum and shows good species specificity. The LAMP assay is therefore an effective method for the quick detection of V. anguillarum both in the laboratory and in the field.

关键词： Loop-Mediated Isothermal Amplification (LAMP) detection assay empA gene Vibrio anguillarum

Surface Microstructure and Component Changes of Chromium-resistant Enterobacter Cloacae CYS-25 Strain

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Structural Chemistry 2008年第1期27卷 15-20页

作者：马晓艳杨春鹏程扬健栗斌李冬松林璋黄丰郑晶 State Key Laboratory of Structural Chemistry Fujian Institute of Research on the Structure of Matter The Chinese Academy of Sciences Fuzhu entry-exit inspection and quarantine bureau

Enterobacter cloacae CYS-25 strain was isolated from a chromate plant. This bacterium was capable of resisting high hexavalent chromium concentration and reducing Cr（VI） under aerobic condition. CrO4^2- stimulated the increase of bacterial size and production of compact convex paths containing chromium on the bacterial surface. The increase of bacterial size was caused by integrative growth but not extracellular polymeric substance hyperplasia. IR and SDS-PAGE analyses showed the extracellular polymeric substance （EPS） components were mainly proteins and had no obvious changes whether the strains were induced by Cr（VI） or not. The EPS was amorphous and contained trivalent chromium. Under CrO4^2- growth condition, the extracellular substance of Enterobacter cloacae CYS-25 strains and Cr（VI） had redox reaction. The products were Cr^3＊-protein complexes which formed a piece of compact convex paths on the surface of bacteria and prevented Cr（VI） from entering into cells.

关键词： Enterobacter cloacae chromium-resistance extracellular polymeric substance(EPS)

Characterization of a Bacteriocin-Like Substance Produced from a Novel Isolated Strain of Bacillus subtilis SLYY-3

在线全文

学校读者我要写书评

暂无评论

Journal of Ocean University of China 2014年第6期13卷 995-999页

作者： LI Junfeng LI Hongfang ZHANG Yuanyuan DUAN Xiaohui LIU Jie College of Chemical Engineering Qingdao University of Science & Technology Yantai entry-exit inspection and quarantine bureau

In the present research, the strain SLYY-3 was isolated from sediments of Jiaozhou Bay, Qingdao, China. The strain SLYY- 3, which produced a bacteriocin-like substance （BLS）, was characterized to be a strain of Bacillus subtillis by biochemical profiling and 16S rDNA sequence analysis. It is the first time to report that Bacillus subtilis from Jiaozhou Bay sediments could produce a BLS. The BLS of B. subtillis SLYY-3 exhibited strong inhibitory activity against gram-positive bacteria （including Staphylococcus aureus and B. subtillis） and some fimgi （including Penicillium glaucum, Aspergillus niger and Aspergillus flavus）. The antimicrobial activity was detected from culture in the exponential growth phase and reached its maximum when culture entered into stationary growth phase. It was thermo-tolerant even when being kept at 100~C for 60 min without losing any activity and stable over a wide pH range from 1.0 to 12.0 while being inactivated by proteolytic enzyme and trypsin, indicating the proteinaceous nature of the BLS. The BLS was purified by precipitation with hydrochloric acid （HC1） and gel filteration （Sephadex G-100）. SDS-PAGE analysis of the extracellular peptides of SLYY-3 revealed a bacteriocin-like protein with a molecular mass of 66 kDa. Altogether, these characteristics indicate the potential of the BLS for food industry as a protection against pathogenic and spoilage microorganisms.

关键词： bacteriocin-like substance Bacillus subtillis SLYY-3 antimicrobial activity food protection purification

Individualized management of pregnant women with high hepatitis B virus DNA levels

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2014年第34期20卷 12056-12061页

作者： Zhao Zhang Chao Chen Zhe Li Ying-Hua Wu Xiao-Min Xiao Department of Obstetrics and Gynecology The First Affiliated Hospital of Jinan University Zhuhai entry-exit inspection and quarantine bureau

Hepatitis B is a major health concern in the Asia-Pacific region, and is endemic in China, Southeast Asia, and Africa. Chronic hepatitis B virus (HBV) infection may cause hepatic cirrhosis and liver cancer. It is estimated that there are more than 350 million chronic HBV carriers worldwide, of whom approximately one quarter will die of chronic hepatitis B-related liver diseases. HBV is transmitted horizontally through blood and blood products or by sexual transmission, and vertically from mother to infant. Perinatal infection is the predominant mode of transmission in countries with a high prevalence of hepatitis B surface antigen (HBsAg) carriage, and perinatal transmission leads to high rates of chronic infection. Therefore, it is important to prevent the mother-to-child transmission (MTCT) of HBV. Research has shown that pregnant women with high HBV DNA levels have an increased risk of MTCT. However, most of the obstetrics guidelines do not make a distinction between pregnant women with high HBV DNA levels and those who are HBsAg positive only. This review addresses the management of pregnant women with high levels of HBV viremia, in terms of antiviral therapy, use of hepatitis B immunoglobulin (HBIG), the combined application of hepatitis B vaccine and HBIG, choice of delivery mode and feeding practices. (C) 2014 Baishideng Publishing Group Inc. All rights reserved.

关键词： Hepatitis B virus Hepatitis B virus DNA High level Management Pregnancy

Ordered proposition fusion based on consistency and uncertainty measurements

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China(Information Sciences) 2017年第8期60卷 117-135页

作者： Dayou LIU Yungang ZHU Ni NI Jie LIU College of Computer Science and Technology Jilin University Key Laboratory of Symbolic Computation and Knowledge Engineering of Ministry of Education Jilin University Beijing entry-exit inspection and quarantine bureau

The fusion of ordered propositions is an important and widespread problem in artificial intelligence,but existing fusion methods have difficulty handling the fusion of ordered propositions. In this paper, we propose a solution based on consistency and uncertainty measurements. The main contributions of this paper are as follows. First, we propose the concept of convexity degree, mean, and center of basic support function to comprehensively describe the basic support function of ordered propositions. Second, we introduce entropy as a measure of uncertainty in the basic support function of ordered propositions. Third, we generalize the indeterminacy of the basic support function and propose a novel method to measure the consistency between two basic support functions. Finally, based on the above researches, we propose a novel algorithm for fusing ordered propositions. Theoretical analysis and experimental results demonstrate that the proposed method outperforms state-of-the-art methods.

关键词： uncertainty processing ordered proposition evidence theory dempster rule of combination information fusion

Trace analysis of anions in organic matrices by ion chromatography coupled with a novel reversed-phase column for on-line sample pretreatment

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2011年第4期22卷 461-464页

作者： Ying Ying Zhong Wen Fang Zhou Xue Ling Zeng Ming Li Ye Yan Zhu Department of Chemistry Zhejiang UniversityHangzhou 310028China Ningbo entry-exit inspection and quarantine bureau Ningbo 315012China

An ion chromatography（IC） system coupled with on-line column-switching technique was used to determine anions ofμg/g levels in organic chemicals of analytical reagent grade.A novel polystyrene-divinylbenzene-carbon nanotube（PS-DVB-CNT） stationary phase was utilized for matrix elimination.A calibration study was conducted by preparing and analyzing eight concentrations（between 10 and 5000μg/L） of eight standards in deionized *** linearity was between 0.9978 and *** the detection limits ranged from 1.54μg/L to 10.02μg/L.A spiking study was performed on two representative organic *** recoveries were between 84.3%and 119.6%.

关键词： Ion chromatography Column switching Trace anions Organic matrix Polymer stationary phase

Liquid-liquid Microextraction Based on Solidification of Floating Organic Drops Coupled with Gas Chromatography for Analyzing Trace Benzene, Toluene and Xylene in Water Samples

在线全文

学校读者我要写书评

暂无评论

Chemical Research in Chinese Universities 2011年第6期27卷 939-943页

作者： ZHANG Mao-sheng HUANG Jia-rong ZHENG Xiang-hua XIE Qiong-fang Department of Chemistry and Environmental Science Zhangzhou Normal University Zhangzhou 363000 P. R. China Zhangzhou entry-exit inspection and quarantine bureau Zhangzhou 363000 P. R. China inspection and quarantine Technical Center Xiamen Entry-Exit Inspection and Quarantine Bureau Xiamen 361012 P. R. China

A new liquid-liquid microextraction method based on the solidification of floating organic drops coupled with gas chromatography was developed for the determination of trace benzene, toluene and xylene（BTX） in water samples. In the microextraction procedure, a microdrop of n-decanol was delivered to the surface of the analytes’ solution, and stirred for a desired time. Following the absolute extraction, the sample vial was cooled in an ice bath for 10 min. The solidified n-decanol was then transferred into a plastic tube and melted naturally; and 1 μL of it was injected into gas chromatography for analysis. Factors relevant to the extraction efficiency were studied and optimized. The optimal experimental conditions were： 15 μL of n-decanol as extractive solvent, 30 mL of solution containing analytes, no salt, the stirring rate 400 r/min, the extraction temperature 30 °C, and the extraction time 30 min. Under those optimized conditions, the detection limit（LOD） of analytes was in a range of 0.05―0.10 ng/mL by the developed method. A good linearity（r0.99） in a calibration range of 0.01―100 μg/mL was obtained. The recoveries of the real samples at different spiked levels of BTX were in the range from 92.2% to 103.4%.

关键词： Gas chromatography Liquid-liquid microextraction Solidified floating organic drop n-Decanol

Synthesis and Crystal Structure of Bis(2-acetylpyridine thiosemicarbazonato)-indium(iii) Perchlorate Hydrate

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Structural Chemistry 2011年第6期30卷 882-886页

作者：王乐边玮玮朱凌倪荣军陶琳 State Key Laboratory of Ceramic Zibo Entry and Exit Inspection and Quarantine Bureau Department of Medical Chemistry Weifang Medical University

The title complex bis（2-acetylpyridine thiosemicarbazonato）-indium（iii） perchlo-rate hydrate has been synthesized by the reaction of 2-acetylpyridine thiosemicarbazide（L） with indium（iii） perchlorate,and its structure was characterized by elemental analysis,IR and single-crystal X-ray *** crystal of the title compound（[In（C8H9N4S）2]ClO4·H2O） belongs to monoclinic,space group P21/c with a = 12.6438（18）,b = 12.6259（18）,c = 15.116（2） nm,β = 105.800（2）°,Z = 4,V = 2321.9（6） nm3,Mr = 618.79,Dc = 1.770 g/cm3,μ = 1.359 mm-1,F（000） = 1240,R = 0.0265 and wR = 0.0675.X-ray analysis indicates that various intriguing hydrogen patterns involving both the solvent intermedia and polyatomic counterions play crucial roles in the self-assembly of functional supramolecules.

关键词： self-assembly cationic complex polyatomic counterion hydrogen-bond

Developmental expression amphioxus Branchiostoma pattern of calmodulin gene in belcheri tsingtauense

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Oceanology and Limnology 2010年第6期28卷 1221-1226页

作者： LUAN Jing GENG Jinpei FANG Shaoqing SU Zhiping ZHANGJingxuan XU Hongyan WANGYing LUMin YIN Weili MENG Guangxiao Department of Marine Biology Ocean university of China Qingdao 266003 China Yantai entry-exit inspection and quarantine bureau Yantai 264000 China

We investigated the developmental expression pattern of AmphiCaM in cephalochordate amphioxus （Branchiostoma belcheri tsingtauense）. We cultured and sampled the animals at different developmental stages （eggs and larvae）, and used in-situ hybridization and northern blotting to document the spatial and temporal changes in AmphiCaM expression. The alimentary tract dominates the development from the late neurula stage to the adult stage. AmphiCaM expression increased significantly in the alimentary tract during the late neurula stage and remained elevated in the adults. Our results indicate that AmphiCaM is involved in the differentiation of the alimentary tract in amphioxus; and furthermore, provide an insight into the change in function of CaM genes during evolution.

关键词： amphioxus calmodulin expression evolution