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The First Asian Conference on Plant Pathology

作者： AbdulMuinAdrian RusmilahSuseno SittiSutarmiTjitrosoma SoetrisnoHadi AbdurraufRambe SidartoWardojo Fakultas Pertanian Institut Pertanian Bogor Bogor Indonesia Fakultas Pertanian Institut Pertanian Bogor Bogor Indonesia Fakultas Matematika dan Iimu Pengetahuan Alam Institut Pertanan bogor Bogor Indonesia Fakultas Kehutanan Institut Pertanian Bogor Bogor Indonesia Fakultas Matematika dan Iimu Pengetahuan Alam Institut Pertanan bogor Bogor Indonesia pusat penelitian perkebunan bogor bogor Indonesia.

＜正＞Inventory of fungal species colonizing root knot nematode （Meloidogyne spp.） infecting soybean roots at four villages in West Java, was made. The infected roots were washed thoroughly by using autoclaved water, then soaked and shaken in 0.5％ NaOCI for 30 seconds, and immediately rinsed with autoclaved water. The adult female nematodes were extracted from the root tissues and subsequently rinsed with autoclaved water and then aseptically placed on water agar in Petri dishes. Five nematodes were placed in each Petri dish and subsequently incubated at room temperature for 2-3 days or longer to allow the development of the fungi colonizing nematodes. The fungal species developing from the individual nematode were transferred to Potato Dextrose Agar for identification. Out of the 265 nematodes collected, 40 individuals were colonized by different fungal species, of which 16 came from Wanajaya samples, 10 from Cipanas samples, 6 from Darmaga samples, and 8 from Sindangbarang samples. Among the isolates obtained, the fungal species that could be identified were Paecilomyces fumosoroseus, P. javanicus, Trichoderma viride, T. harzianum Aspergillus niger, A. parasiticus, A. fiavus, Gliocladium roseum, G. catenulatum and Fusarium fiocciferum. Some of them are suspected to be potential for controlling Meloidogyne spp. on soybean.

关键词： Meloidogyne spp. fungal species colonization soybean West Java

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Diagnostic description and geographic distribution of four new cryptic species of the blue-spotted maskray species complex(Myliobatoidei:Dasyatidae;Neotrygon spp.)based on DNA sequences

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Journal of Oceanology and Limnology 2018年第3期36卷 827-841页

作者： Philippe BORSA Irma S.ARLYZA Thierry B.HOAREAU Kang-Ning SHEN Institut de Recherche pour le Développement(IRD) UMR 250"Ecologie Marine Tropicale des Oceans Pacifique et Indien"BP A598848 NouméaNew Caledonia Lembaga Ilmu Pengetahuan Indonesia(LIPI) Pusat Penelitian Oseanografi(P20)JakartaIndonesia Molecular Ecology and Evolution Programme Department of GeneticsUniversity ofPretoriaPretoriaSouth Africa Aquatic Technology Laboratories Agricultural Technology Research InstituteTaiwanChina

Nine morphologically similar but genetically distinct lineages in the blue-spotted maskray species complex, previously Neotrygon kuhlii(Müller and Henle) qualify as cryptic species. Four of these lineages have been previously described as N eotrygon australiae Last, White and Séret, N eotrygon caeruleopunctata Last, White and Séret, N eotrygon orientale Last, White and Séret, and N eotrygon varidens(Garman), but the morphological characters used in the descriptions off ered poor diagnoses and their geographic distributions were not delineated precisely. The objective of the present work is to complete the description of the cryptic species in the complex. Here, an additional four lineages are described as new species on the basis of their mitochondrial DNA sequences: Neotrygon bobwardi, whose distribution extends from the northern tip of Aceh to the western coast of Sumatera; N eotrygon malaccensis, sampled from the eastern part of the Andaman Sea and from the Malacca Strait; N eotrygon moluccensis, from the eastern half of the Banda Sea; and Neotrygon westpapuensis from the central portion of northern West Papua. The geographic distributions of N. australiae, N. coeruleopunctata, N. orientale, and N. varidens are updated. For each species, a diagnosis is provided in the form of a combination of private or partly-private nucleotides at 2–4 nucleotide sites along a 519-base pair fragment of the CO1 gene. We believe that the present taxonomic revision will provide information relevant to the sound management and conservation of cryptic species of the blue-spotted maskray in the Coral Triangle region.

关键词： molecular taxonomy diagnosis distribution

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