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Magnetic bioassembly platforms towards the generation of extracellular vesicles from human salivary gland functional organoids for epithelial repair

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Bioactive Materials 2022年第12期7卷 151-163页

作者： Ajjima Chansaenroj Christabella Adine Sawanya Charoenlappanit Sittiruk Roytrakul Ladawan Sariya Thanaphum Osathanon Sasitorn Rungarunlert Ganokon Urkasemsin Risa Chaisuparat Supansa Yodmuang Glauco R.Souza Joao N.Ferreira Avatar Biotechnologies for Oral Health and Healthy Longevity Research Unit Faculty of DentistryChulalongkorn UniversityBangkok10330Thailand Faculty of Dentistry National University of Singapore119077SingaporeSingapore Department of Biomedical engineering Faculty of EngineeringNational University of Singapore119077SingaporeSingapore Functional Ingredients and Food Innovation Research Group National Center for Genetic Engineering and BiotechnologyNational Science and Technology Development AgencyPathum Thani12120Thailand The Monitoring and Surveillance Center for Zoonotic Diseases in Wildlife and Exotic Animals Faculty of Veterinary ScienceMahidol UniversityNakhon Pathom73170Thailand Department of Anatomy Faculty of DentistryChulalongkorn UniversityBangkok10330Thailand Department of Preclinical and Applied Animal Science Faculty of Veterinary ScienceMahidol UniversityNakhon Pathom73170Thailand Research Affairs Faculty of MedicineChulalongkorn UniversityBangkok10330Thailand University of Texas Health Sciences Center at Houston HoustonTX77030USA Nano3D Biosciences inc. HoustonTX77030USA Greiner Bio-One North America inc MonroeNC28110USA

Salivary glands(SG)are exocrine organs with secretory units commonly injured by ***-engineered organoids and extracellular vesicles(EV)are currently under investigation as potential strategies for SG ***,three-dimensional(3D)cultures of SG functional organoids(SGo)and human dental pulp stem cells(hDPSC)were generated by magnetic 3D bioassembly(M3DB)*** growth factor 10(FGF10)was used to enrich the SGo in secretory epithelial *** 11 culture days via M3DB,SGo displayed SG-specific acinar epithelial units with functional properties upon *** consistently develop 3D hDPSC in vitro,3 culture days were sufficient to maintain hDPSC undifferentiated genotype and phenotype for EV *** isolation was performed via sequential centrifugation of the conditioned media of hDPSC and SGo *** were characterized by nanoparticle tracking analysis,electron microscopy and *** were in the exosome range for hDPSC(diameter:88.03±15.60 nm)and for SGo(123.15±63.06 nm).Upon ex vivo administration,exosomes derived from SGo significantly stimulated epithelial growth(up to 60%),mitosis,epithelial progenitors and neuronal growth in injured SG;however,such biological effects were less distinc.ive with the ones derived from ***,these exosome biological effects were investigated by proteomic *** spectrometry profiling of SGo exosomes predicted that cellular growth,development and signaling was due to known and undocumented molecular targets downstream of *** were identified as one of the novel targets requiring further ***,M3DB platforms can generate exosomes with potential to ameliorate SG epithelial damage.

关键词： Salivary gland Hyposalivation Human dental pulp stem cells Magnetic bioassembly Organoids Exosome

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Decomposition Methods for Manufacturing System Scheduling:A Survey

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IEEE/CAA Journal of Automatica Sinica 2018年第2期5卷 389-400页

作者： Fajun Yang Kaizhou Gao Ian Ware Simon Yuting Zhu Rong Su School of Electrical and Electronic engineering at Nanyang Technological University Singapore 639798Singapore IEEE

Manufacturing is the application of labor, tools,machines, chemical and biological processing, to an original raw material by changing its physical and geometrical characteristics, in order to make finished products. Sinc. the first industrial revolution, to accommodate the large-scale production,tremendous changes have happened to manufacturing through the innovations of technology, organization, management, transportation and communication. This work first reviews the highvolume low-mix process by focusing on the quantity production,transfer line and single model assembly line. Then, it reviews the high-volume high-mix process. For such a process type,mixed/multi model assembly line is usually adopted. Hence,two main decisions on them, i.e., balancing and, sequencing are reviewed. Thereafter, it discusses the low-volume high-mix process in detail. Then, technology gap and future work is discussed, and at last, conclusions are given.

关键词： Index Terms-Assembly line high-mix high-volume low-mix low-volume manufacturing processes.

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The Genome of Medicinal Plant Macleaya cordata Provides New Insights into Benzylisoquinoline Alkaloids Metabolism

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Molecular Plant 2017年第7期10卷 975-989页

作者： Xiubin Liu Yisong Liu Peng Huang Yongshuo Ma Zhixing Qing Qi Tang Huifen Cao Pi Cheng Yajie Zheng Zejun Yuan Yuan Zhou Jinfeng Liu Zhaoshan Tang Yixiu Zhuo Yancong Zhang Linlan Yu Jialu Huang Peng Yang Qiong Peng dinbo Zhang Wenkai Jiang Zhonghua Zhang Kui Lin Dae-Kyun Ro Xiaoya Chen Xingyao Xiong Yi Shang Sanwen Huang Jianguo Zeng National and Local Union engineering Research Center of Veterinary Herbal Medicine Resource and Initiative Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients Horticulture and Landscape College Veterinary Medicine College Hunan Agricultural University Changsha 410128 China Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture Sino-Dutch Joint Laboratory of Horticultural Genomics Beijing 100081 China Agricuitural Genome Institute at Shenzhen Chinese Academy of Agricultural Sciences Shenzhen 518124 China School of Pharmacy Hunan University of Chinese Medicine Changsha 410208 China MOE Key Laboratory for Biodiversity Science and Ecological engineering and College of Life Sciences Beijing Normal University Beijing 100875 China Micolta Bioresource inc. Changsha 410016 China Herbal Extract engineering Research Center Changsha 410331 China Biotechnology Research Center Hunan Academy of Agricultural Sciences Changsha 410125 China Novogene Bioinformatics Institute Beijing 100083 China Department of biological Sciences University of Calgary Calgary T2N1N4 Canada National Key Laboratory of Plant Molecular Genetics National Plant Gene Research Center Institute of Plant Physiology and Ecology Shanghai Institutes forBiological Sciences Chinese Academy of Sciences Shanghai 200032 China Plant Science Research Center Shanghai Chenshan Botanical Garden Shanghai 201602 China

The overuse of antibiotics in animal agriculture and medicine has caused a series of potential threats to public health. Macleaya cordata is a medicinal plant species from the Papaveraceae family, providing a safe resource for the manufacture of antimicrobial feed additive for livestock. The active constituents from M. cordata are known to inc.ude benzylisoquinoline alkaloids （BIAs） such as sanguinarine （SAN） and chelerythrine （CHE）, but their metabolic pathways have yet to be studied in this non-model plant. The active biosynthesis of SAN and CHE in M. cordata was first examined and confirmed by feeding ^13C-labeled tyrosine. To gain further insights, we de novo sequenced the whole genome of M. cordata, the first to be sequenced from the Papaveraceae family. The M. cordata genome covering 378 Mb encodes 22,328 predicted protein-coding genes with 43.5% being transposable elements. As a member of basal eudicot, M. cordata genome lacks the paleohexaploidy event that occurred in almost all eudicots. From the genomics data, a complete set of 16 metabolic genes for SAN and CHE biosynthesis was retrieved, and 14 of their biochemical activities were validated. These genomics and metabolic data show the conserved BIA metabolic pathways in M. cordata and provide the knowledge foundation for future productions of SAN and CHE by crop improvement or microbial pathway reconstruction.

关键词： Macleaya cordata genome Benzylisoquinoline alkaloids Secondary metabolism Sanguinarine Chelerythrine Isotopic labeling

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Review of in-vivo characterisation of corneal biomechanics

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Medicine in Novel Technology and Devices 2021年第3期11卷 34-39页

作者： Bernardo T.Lopes FangJun Bao JunJie Wang XiaoYu Liu LiZhen Wang Ahmed Abass Ashkan Eliasy Ahmed Elsheikh School of engineering University of LiverpoolLiverpoolUK Department of Ophthalmology Federal Univerisity of Sao Paulo(UNIFESP)Sao PauloBrazil Eye Hospital Wenzhou Medical UniversityWenzhouChina The Institution of Ocular Biomechanics Wenzhou Medical UniversityWenzhouChina Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education School of Biological Science and Medical EngineeringBeihang UniversityBeijingChina Beijing Advanced Innovation Centre for Biomedical engineering Beihang UniversityBeijingChina National Institute for Health Research(NIHR)Biomedical Research Centre for Ophthalmology Moorfields Eye Hospital NHS Foundation Trust and UCL Institute of OphthalmologyLondonUK

The study of corneal biomechanics in vivo has been evolving fast in recent *** an organised corneal structure is necessary for its transparency,resistance to occasional external insults and bearing the intraocular pressure(IOP),which several clinically relevant events can *** review focuses on three techniques that are available for clinical use,namely the Ocular Response Analyzer(Reichert Ophthalmic Instruments,Buffalo,NY,USA),the Corvis ST(Oculus Optikgerate GmbH,Wetzlar,Germany)and the Brillouin Optical Scattering System(Intelon Optics inc.,Lexington,MA,USA).The princ.ples and the main parameters of each device are discussed along with their strategies to improve accuracy in the IOP measurement,corneal ectasia diagnosis,evaluation of corneal cross-linking procedures,and planning of corneal refractive surgeries.

关键词： Corneal biomechanics Corneal diagnosis Intraocular pressure Corneal ectasia Corneal cross linking

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Scalable and automated CRISPR-based strain engineering using droplet microfluidics

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Microsystems & Nanoengineering 2022年第2期8卷 227-236页

作者： Kosuke Iwai Maren Wehrs Megan Garber Jess Sustarich Lauren Washburn Zachary Costello Peter W.Kim David Ando William R.Gaillard Nathan J.Hillson Paul D.Adams Aindrila Mukhopadhyay Hector Garcia Martin Anup K.Singh Technology Division DOE Joint BioEnergy InstituteEmeryvilleCA 94608USA Biotechnology and Bioengineering Department Sandia National LaboratoriesLivermoreCA 94550USA Biofuels and Bioproducts Division DOE Joint BioEnergy InstituteEmeryvilleCA 94608USA biological Systems and engineering Division Lawrence Berkeley National LaboratoryBerkeleyCA 94720USA Molecular Biophysics and Integrated Bioimaging Division Lawrence Berkeley National LaboratoryBerkeleyCA 94720USA Department of Bioengineering University of CaliforniaBerkeleyCA 94720USA BCAM Basque Center for Applied MathematicsBilbao 48009Spain

We present a droplet-based microfluidic system that enables CRISPR-based gene editing and high-throughput screening on a *** microfluidic device contains a 10x10 element array,and each element contains sets of electrodes for two electric field-actuated operations:electrowetting for merging droplets to mix reagents and electroporation for *** device can perform up to 100 genetic modification reactions in parallel,providing a scalable platform for generating the large number of engineered strains required for the combinatorial optimization of genetic pathways and predictable *** demonstrate the system's capabilities through the CRISPR-based engineering of two test cases:(1)disruption of the function of the enzyme galactokinase(galK)in£coli and(2)targeted engineering of the glutamine synthetase gene(glnA)and the blue-pigment synthetase gene(bpsA)to improve indigoidine production in£coli.

关键词： operations generating fluid

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Recycling agricultural plastic mulch:limitations and opportunities in the United States

Circular Agricultural Systems

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Circular Agricultural Systems 2024年第1期4卷 31-41页

作者： Kwabena A.Sarpong Funmilayo A.Adesina Lisa W.DeVetter Kun Zhang Kevin DeWhitt Karl R.Englund Carol Miles Washington State University Mount Vernon Northwestern Washington Research and Extension Center Mount VernonWA 98273USA Washington State University Composite Materials and engineering Center Pullman WA 99164-5815USA California State University ChicoCA 95929USA PDO Technologies Inc.GervaisOR 97026-8742USA

Plastic mulches have become an essential component of modern agriculture sinc. their introduction in the ***,disposal of plastic mulches poses serious environmental challenges as plastics that are not considered biodegradable or compostable can take several hundred years to *** year in the United States,only 9%of overall plastic waste is recycled while 79%is accumulated in landfills or the natural *** of plastic mulch is especially constrained due to the contamination that results from their use in ***,recovered mulches are reported to have 30%–80%surface contamination,primarily from soil and plant *** mulch waste is concentrated in areas where they are used and can provide logistical opportunities to the plastic recycling *** recycling inc.udes mechanical,advanced(chemical and thermal),and biological methods,that may all be used for polyethylene(PE).Most plastic is recycled using the mechanical method,while advanced and biological methods are promising but face significant financial and technical *** all recycling methods,strategies are needed for managing surface contamination to realize the recycling potential of plastic mulch.

关键词： cycling debris primarily

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Single-cell RNA-seq unveils critical regulators of human FOXP3^+regulatory T cell stability

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Science Bulletin 2020年第13期65卷 1114-1124,M0004页

作者： Gang Yi Yi Zhao Feng Xie Fuxiang Zhu Ziyun Wan Jingwan Wang Xie Wang Kai Gao Lixia Cao Xinyang Li Chen Chen Yashu Kuang Xiu Qiu Huanming Yang Jian Wang Bing Su Lei Chen Wei Zhang Yong Hou Xun Xu Yinyan He Andy Tsun Xiao Liu Bin Li Shanghai Institute of Immunology Shanghai Jiao Tong University School of MedicineShanghai 200025China Department of Immunology and Microbiology Shanghai Jiao Tong University School of MedicineShanghai 200025China BGI-Shenzhen Shenzhen 518083China School of Biology and biological engineering South China University of TechnologyGuangzhou 510006China School of Mathematical Sciences University of Chinese Academy of SciencesBeijing 100049China Department of Obstetrics and Gynaecology Shanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai 200080China Division of Birth Cohort Study Guangzhou Women and Children’s Medical CenterGuangzhou Medical UniversityGuangzhou 510006China James D.Watson Institute of Genome Sciences Hangzhou 310058China Biotheus inc. Zhuhai 519080China

The heterogeneity and plasticity of T lymphocytes is critical for determining immune response *** regulatory T(Treg)cells are commonly characterized by stable FOXP3 expression and have reported to exhibit heterogeneous phenotypes under inflammatory ***,the interplay between inflammation and Treg cell suppressive activity still remains ***,we utilized singlecell RNA sequencing to investigate how human Treg cells respond to the pro-inflammatory cytokine interleukin-6(IL-6).We observed that Treg cells divided into two subpopulations after IL-6 ***àunstable Treg cells lost FOXP3 expression and gained an effector-like T cell phenotype,whereas TIGIT+Treg cells retained robust suppressive *** cell transcriptome analysis revealed a spectrum of cellular states of IL-6-stimulated Treg cells and how cytochrome P450 family 1 subfamily A member 1(CYP1A1)is a crucial regulator of Treg cell suppressive capability and ***1A1-deficient human Treg cells developed a Th17-like phenotype after IL-6 *** findings implicate CYP1A1 as a previously unidentified regulator of Treg cells that may have target potential for clinical application for biotherapies.

关键词： Treg cell scRNA-seq Inflammation Interleukin-6 Heterogeneity Cytochrome P4501A1

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Downwash airflow field distribution characteristics and their effect on the spray field distribution of the DJI T30 six-rotor plant protection UAV

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International Journal of Agricultural and biological engineering 2023年第2期16卷 10-22页

作者： Haiyan Zhang Sheng Wen Chunling Chen Qi Liu Tongyu Xu Shengde Chen Yubin Lan College of Information and Electrical engineering Shenyang Agricultural UniversityShenyang 110866China College of engineering South China Agricultural UniversityGuangzhou 510642China College of Agricultural engineering and Food Science Shandong University of TechnologyZibo 255022ShandongChina College of Electronic engineering(College of Artificial Intelligence) South China Agricultural UniversityGuangzhou 510642China National Center for International Collaboration Research on Precision Agricultural Aviation Pesticides Spraying Technology Guangzhou 510642China

Spray characteristics are the fundamental factors that affect droplet transportation downward,deposition,and *** downwash airflow field of the Unmanned Aviation Vehicle(UAV)primarily influences droplet deposition and drift by changing the spray *** study focused mainly on the effect of the downwash airflow field of the UAV and nozzle position on the droplet spatial distribution and velocity distribution,which are two factors of spray *** study the abovementioned characteristics,computational fluid dynamics based on the lattice Boltzmann method(LBM)was used to simulate the downwash airflow field of the DJI T30 six-rotor plant protection UAV at different rotor rotational speeds(1000-1800 r/min).A particle image velocimetry system(PIV)was utilized to record the spray field with the downwash airflow field at different rotational speeds of rotors(0-1800 r/min)or different nozzle positions(0,0.20 m,0.35 m,and 0.50 m from the motor).The simulation and experimental results showed that the rotor downwash airflow field exhibited the‘dispersion-shrinkage-redispersion’development *** the initial dispersion stage of rotor airflow,there were obvious high-vorticity and low-vorticity regions in the rotor downwash airflow ***,the low-vorticity region was primarily concentrated below the motor,and the high-vorticity region was mainly focused in the middle area of the ***,the Y-direction airflow velocity fluctuated at 0.4-1.2 m under the *** the rotor airflow developed to 3.2 m below the rotor,the Y-direction airflow velocity showed a slight *** 3.2 m from the rotor,the Y-direction airflow velocity started to drastically ***,it is recommended that the DJI T30 plant protection UAV should not exceed 3.2 m in flight height during field spraying *** rotor downwash airflow field caused the nozzle atomization angle,droplet concentration,and spray field width to decrease while inc

关键词： downwash airflow spray field distribution plant protection UAV characteristics

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Isolation of intact bacteria from blood by selective cell lysis in a microfluidic porous silica monolith

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Microsystems & Nanoengineering 2019年第1期5卷 418-428页

作者： Jung Y.Han Michael Wiederoder Don L.DeVoe Department of Mechanical engineering University of MarylandCollege ParkMD 20742USA Department of Chemical and Biomolecular engineering University of MarylandCollege ParkMD 20742USA Fischell Department of Bioengineering University of MarylandCollege ParkMD 20742USA

Rapid and efficient isolation of bacteria from complex biological matrices is necessary for effective pathogen identification in emerging single-cell ***,we demonstrate the isolation of intact and viable bacteria from whole blood through the selective lysis of blood cells during flow through a porous silica *** mechanical hemolysis is achieved while providing passage of intact and viable bacteria through the monoliths,allowing size-based isolation of bacteria to be performed following selective lysis.A process for synthesizing large quantities of discrete capillary-bound monolith elements and millimeter-scale monolith bricks is described,together with the seamless integration of individual monoliths into microfluidic *** impact of monolith morphology,geometry,and flow conditions on cell lysis is explored,and flow regimes are identified wherein robust selective blood cell lysis and intact bacteria passage are achieved for multiple gram-negative and gram-positive *** technique is shown to enable rapid sample preparation and bacteria analysis by single-cell Raman *** selective lysis technique presents a unique sample preparation step supporting rapid and culture-free analysis of bacteria for the point of care.

关键词： silica porous quantities

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Comparing biomarkers and proteomic fingerprints for classification studies

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Journal of Biomedical Science and engineering 2013年第4期6卷 453-465页

作者： Brian T. Luke Jack R. Collins Jens K. Habermann DaRue A. Prieto Timothy D. Veenstra Thomas Ried Advanced Biomedical Computing Center Information Systems Program SAIC-Frederick Inc. Frederick National Laboratory for Cancer Research Frederick USA Genetics Branch National Cancer Institute NIH Bethesda USA Laboratory for Surgical Research Department of Surgery University Hospital Schleswig-Holstein Campus Luebeck Luebeck Germany Laboratory of Proteomics and Analytical Technologies Advanced Technology Program SAIC-Frederick Inc. Frederick National Laboratory for Cancer Research Frederick USA

Early disease detection is extremely important in the treatment and prognosis of many diseases, especially cancer. Often, proteomic fingerprints and a pattern recognition algorithm are used to classify the pathological condition of a given individual. It has been argued that accurate classification of the existing data implies an underlying biological significance. Two fingerprint-based classifiers, decision tree and medoid classification algorithm, and a biomarker-based classifier were examined using a published dataset of mass spectral peaks from 81 healthy individuals and 78 individuals with benign prostate hyperplasia (BPH). For all three methods, classifiers were constructed using the original data and the data after permuting the labels of the samples (BPH and healthy). The fingerprint-based classifiers produced accurate results for the original data, though the peaks used in a given classifier depended upon which samples were placed in the training set. Accurate results were also obtained for the dataset with permuted labels. In contrast, only three unique peaks were identified as putative biomarkers, producing a small number of reasonably accurate biomarker-based classifiers. The dataset with permuted labels was poorly classified. Sinc. fingerprint-based classifiers accurately classified the dataset with permuted labels, the argument for biological significance from a fingerprint-based classifier must be questioned.

关键词： Biomarker Classifier Proteomic Fingerprint

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