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Overexpressed NEDD8 as a potential therapeutic target in esophageal squamous cell carcinoma

Cancer Biology & Medicine 2022年第4期19卷 504-517页

作者： Jingrong Xian Shiwen Wang Yanyu Jiang Lihui Li Lili Cai Ping Chen Yue Liu Xiaofei Zeng Guoan Chen Chen Ding Robert MHoffman Lijun Jia Hu Zhao Yanmei Zhang Department of Laboratory Medicine Huadong Hospital Affiliated to Fudan UniversityShanghai 200040China Cancer Institute Longhua HospitalShanghai University of Traditional Chinese MedicineShanghai 200032China Research Center on Aging and Medicine Fudan UniversityShanghai 200040China Shanghai Key Laboratory of Clinical Geriatric Medicine Shanghai 200040China Department of Basic Science of Oncology College of Basic Medical SciencesZhengzhou UniversityCollaborative Innovation Center of Henan Province for Cancer ChemopreventionZhengzhou 450001China School of Medicine Southern University of Science and TechnologyShenzhen 518055China State Key Laboratory of Genetic engineering Human Phenome InstituteInstitutes of Biomedical SciencesSchool of Life SciencesZhongshan HospitalFudan UniversityShanghai 200032China State Key Laboratory of Cell Differentiation and Regulation Henan International Joint Laboratory of Pulmonary FibrosisHenan Center for Outstanding Overseas Scientists of Pulmonary FibrosisCollege of Life ScienceInstitute of Biomedical ScienceHenan Normal UniversityXinxiang 453007China Department of Surgery University of CaliforniaSan Diego 92101USA Anticancer inc. San Diego 92101USA

Objective:The hyperactivated neddylation pathway plays an important role in tumorigenesis and is emerging as a promising anticancer *** aimed to study whether NEDD8(neural precursor cell expressed,developmentally down-regulated 8)might serve as a therapeutic target in esophageal squamous cell carcinoma(ESCC).Methods:The clinical relevance of NEDD8 expression was evaluated by using The Cancer Genome Atlas(TCGA)database and tissue ***8-knockdown ESCC cells generated with the CRISPR/Cas9 system were used to explore the anticancer effects and *** proteomic analysis was used to examine the variations in NEDD8 knockdown-induced biological *** cell cycle and apoptosis were assessed with fluorescence activated cell sorting.A subcutaneous-transplantation mouse tumor model was established to investigate the anticancer potential of NEDD8 silencing in ***:NEDD8 was upregulated at both the mRNA and protein expression levels in ESCC,and NEDD8 overexpression was associated with poorer overall patient survival(mRNA level:P=0.028,protein level:P=0.026,log-rank test).Downregulation of NEDD8 significantly suppressed tumor growth both in vitro and in *** proteomic analysis revealed that downregulation of NEDD8 induced cell cycle arrest,DNA damage,and apoptosis in ESCC *** studies demonstrated that NEDD8 knockdown led to the accumulation of cullin-RING E3 ubiquitin ligases(CRLs)substrates through inactivation of CRLs,thus suppressing the malignant phenotype by inducing cell cycle arrest and apoptosis in *** experiments demonstrated that the induction of apoptosis after NEDD8 silencing was attenuated by DR5 ***:Our study elucidated the anti-ESCC effects and underlying mechanisms of NEDD8 knockdown,and validated NEDD8 as a potential target for ESCC therapy.

关键词： NEDD8 esophageal squamous cell carcinoma cullin-RING E3 ubiquitin ligases apoptosis anticancer target

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Optimization of protein crystallization condition using viscous materials for the space experiments

Optimization of protein crystallization condition using visc...

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10～(th) International Conference on the Crystallization of biological Macromlecules

作者： Yamanaka M. Inaka K. Sugiyama S. Sato M. Takahashi S. Tanaka H. Japan Space Utilization Promotion Center Nishi-WasedaShinjyuku-kuTokyo169-8624 Japan Maruwa Food Industries Inc.Tsutsui-choYamatokoriyama-shiNara639-1123Japan Maruwa Food Industries Inc.Tsutsui-choYamatokoriyama-shiNara639-1123Japan Japan Aerospace Exploration Agency SengenTsukuba-shiIbaraki305-8505Japan Japan Space Utilization Promotion Center Nishi-WasedaShinjyuku-kuTokyo169-8624 Japan Japan Space Utilization Promotion Center Nishi-WasedaShinjyuku-kuTokyo169-8624 Japan

＜正＞ It is known that the depletion zone of the protein and impurities are formed around the crystal during the protein crystal growth if the diffusive field around the crystal is not disturbed [1]. With the existence of the depletion zone, the growth speed of the crystals may become decreased and the impurity uptake may be suppressed to give much ordered crystals. It is well known that microgravity environment can reduce convective fluid motion which is supposed to disturb the depletion zones [2]. Therefore we expected that the crystals grown in space can obtain better quality than the ones grown on the ground.

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Lysosomal Exocytosis in Schwann Cells Contributes to Axon Remyelination

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神经损伤与功能重建 2012年第3期7卷 216-223页

作者： GANG CHEN ZHIJUN ZHANG ZHONGYA WEI QIONG CHENG XIA LI WEI LI SHUMIN DUAN AND XIAOSONG GU Jiangsu Key Laboratory of Neuroregeneration Nantong UniversityNantong226001JiangsuChina Institute of Neuroscience Shanghai Institute for Biological SciencesChinese Academy of SciencesShanghai 200031China Neuroscience and Stem Cell engineering Research Center Med-X Research Institute of Shanghai Jiao Tong UniversityShanghai 200031China Department of Neurobiology Zhejiang University School of MedicineHangzhou 310058China Institute of Genetics and Developmental Biology Chinese Academy of SciencesBeijing 100101China

髓鞘形成是一个包括协同性的胞吐、内吞、mRNA转运和细胞骨架的动态变化的复杂过程。尽管髓鞘的异常在溶酶体贮积症中很常见,但对溶酶体在髓鞘形成和维持中所扮演的角色仍不清楚。本文发现Schwann细胞中的晚期内涵体/溶酶体包含大量的... 详细信息

髓鞘形成是一个包括协同性的胞吐、内吞、mRNA转运和细胞骨架的动态变化的复杂过程。尽管髓鞘的异常在溶酶体贮积症中很常见,但对溶酶体在髓鞘形成和维持中所扮演的角色仍不清楚。本文发现Schwann细胞中的晚期内涵体/溶酶体包含大量的髓鞘蛋白P0,含量占超过一半的外周神经系统中的致密髓鞘的总蛋白并且在不同的刺激下表现出Ca2+依赖性的胞吐作用。Rab27a(一种将分泌溶酶体运输至细胞膜的小GTP酶)下调,极大地阻碍了Schwann细胞中的溶酶体胞吐作用,减少了再生坐骨神经的髓鞘形成。这些发现强调了Schwann细胞中溶酶体的新角色,提示调节Schwann细胞中的溶酶体胞吐作用在外周神经系统中有很重要的生理和病理意义。

关键词： Schwann细胞溶酶体胞吐作用髓鞘

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Corrigendum to“CRISPR-detector:fast and accurate detection,visualization,and annotation of genome-wide mutations induced by genome editing events”[Journal of Genetics and Genomics(2023)563-572]

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Journal of Genetics and Genomics 2023年第10期50卷 813-813页

作者： Lei Huang Dan Wang Haodong Chen Jinnan Hu Xuechen Dai Chuan Liu Anduo Li Xuechun Shen Chen Qi Haixi Sun Dengwei Zhang Tong Chen Yuan Jiang College of Life Sciences University of Chinese Academy of SciencesBeijing 100049China BGI-Shenzhen ShenzhenGuangdong 518083China BNU-HKBU United International College ZhuhaiGuangdong 519087China Sentieon inc San JoseCA 94042USA School of Biology and biological engineering South China University of TechnologyGuangzhouGuangdong 510006China National Resource Center for Chinese Materia Medica China Academy of Chinese Medical SciencesBeijing 100700China

This corrigendum clarifies information in the article“CRISPR-detector:fast and accurate detection,visualization,and annotation of genomewide mutations induced by genome editing events”by Huang et al.(2023).In the figure legend of Fig.S1,the sentence“CRISPR-detector failed to report insertions larger than 72 bp while CRISPResso2 failed to report insertions larger than 53 bp.”should be corrected into“CRISPR-detector failed to report deletions larger than 71 bp while CRISPResso2 failed to report deletions larger than 53 bp.”

关键词： al. Cor insertion

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Automated Approaches to Protein Crystallization Utilizing Minimal Sample Volumes

Automated Approaches to Protein Crystallization Utilizing Mi...

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10～(th) International Conference on the Crystallization of biological Macromlecules

作者： Doneen B. Khoja H. Oza U. McIntosh B Genomic Solutions Inc.17851 Sky Park CircleIrvineCA 92614（USA） Genomic Solutions Inc.17851 Sky Park CircleIrvineCA 92614（USA） Genomic Solutions Inc.17851 Sky Park CircleIrvineCA 92614（USA） Genomic Solutions Inc.17851 Sky Park CircleIrvineCA 92614（USA）

＜正＞ Crystallization screening strategies are now routinely employing robotic dispensing systems to inc.ease throughput. With the inc.easing number of screening combinations needed to identify optimal crystallization conditions, the requirement for protein usage can be quite large. Expression and purification of sufficient amounts of protein are both costly and time consuming. To efficiently screen large numbers of conditions, the quantity of protein required for each test must be reduced. Utilizing the Honeybee systems, this poster shows that the amount of protein used in standard protocols can be reduced without sacrificing accuracy and precision.

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Thermophilic anaerobic digestion of cattle manure reduces seed viability for four weed species

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International Journal of Agricultural and biological engineering 2012年第1期5卷 71-75页

作者： R.E.Eckford J.C.Newman X.Li P.R.Watson Technology Future Bag 4000VegrevilleAlbertaCanadaT0B 2S0 XY Green Carbon inc 1124111A ST EdmontonAlbertaCanada

Anaerobic digestion of manure and other biowaste has been gaining public attention for producing biogas as a renewable *** digestate materials after harvesting biogas available will be used as biofertilizers,soil conditioners and amendments for land ***,digestate is required to be free of weed *** effect of anaerobic digestion on the survival of weed seeds has not been studied *** study examined four weed seeds,wild oats(Avena fatua L.),wild buckwheat(Polygonum convolvulus L.),wild mustard(Sinapis arvensis(DC.)***)and volunteer canola(Brassica napus L.)that were placed in batch cultures with feedlot cattle manure at 55℃for 7 and 24 *** results showed that after being subjected to anaerobic digestion for 7 hours,wild oats,volunteer canola and wild mustard had zero *** buckwheat had remaining viable seed after the 7 and 24 hour anaerobic digestion treatment as shown by the tetrazolium ***,the remaining viable wild buckwheat seeds were considered to be inc.pable of normal growth and development.

关键词： weed seeds anaerobic digestion Avena fatua Polygonum convolvulus Brassica napus Brassica kaber

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Risk factors and molecular epidemiology of canine rabies in Beijing

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One Health Advances 2023年第1期1卷 175-183页

作者： Zhen Wang Qingzhi Liu Li Mei Junlin Guo Xiaolong Gao Bi Zhang Chang Cai Yipeng Sun Xiaoyu Feng Yongqiang Wang National Key Laboratory of Veterinary Public Health Security Key Laboratory for Prevention and Control of Avian Influenza and Other Major Poultry Diseases and Key Laboratory of Animal Epidemiology of Ministry of Agriculture and Rural AffairsCollege of Veterinary MedicineChina Agricultural UniversityBeijing 100193China State Key Laboratories of Agrobiotechnology Department of Microbiology and ImmunologyCollege of Biological ScienceChina Agricultural UniversityBeijing 100193China Department of Biostatistics University of Michigan-Ann ArborAnn ArborMI 48109USA Beijing Animal Disease Prevention and Control Center Beijing 100260China New Ruipeng Pet Group inc Beijing 100000China College of Science HealthEngineering&EducationMurdoch UniversityPerthWA 6149Australia National Key Laboratory of Veterinary Public Health Security Microbiology and Immunology Department of Preventive Veterinary MedicineCollege of Veterinary MedicineChina Agricultural UniversityBeijing 100193China

Rabies is a serious public health problem in Asia and *** 99%of cases result from a bite by an infected *** high rabies risk countries are generally characterized by rapid urbanization;however,the prevalence and risk factors for rabies in urban dogs in developing countries remain ***,the capital of China,has been undergoing rapid urbanization,and implemented compulsory rabies immunization for dogs on October 1,*** evaluated the effects of this immunization policy and investigated the dynamics and determinants of the spread of dog rabies virus(RABV)in *** 2013 and 2018,the positive rate of rabies in biting dogs declined significantly;79.6%of these were stray *** spatiotemporal diffusion for RABV presents a more central distribution within Beijing and a surrounding dissemination of virus lineage toward suburban areas,indicating that rabies is self-sustaining in *** RABV phylogeographic reconstructions and logistic regression analysis of rabies cases revealed that rabies tended to remain in commercial areas,high-rise residences,demolition areas,and high-GDP,high-population and high-accessibility *** areas,where many residents have abandoned their pets,are recognized as high-risk areas for *** findings reveal that the implementation of the immunization policy in Beijing has been effective in prevention and control of canine rabies,and because the prevalence of canine rabies results from multiple factors,emphasize the important role of multi-sectoral cooperation in rabies prevention and control.

关键词： Rabies Urban areas Prevalence Risk factors

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3D-printed gelatin methacrylate (GelMA)/ silanated silica scaffold assisted by two-stage cooling system for hard tissue regeneration

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Regenerative Biomaterials 2021年第2期8卷 50-63页

作者： Eunjeong Choi Dongyun Kim Donggu Kang Gi Hoon Yang Bongsu Jung MyungGu Yeo Min-Jeong Park SangHyun An KyoungHo Lee Jun Sik Kim Jong Chul Kim Woonhyeok Jeong Hye Hyun Yoo Hojun Jeon Research Institute of Additive Manufacturing and Regenerative Medicine Baobab Healthcare Inc55 Hanyangdaehak-RoAnsanGyeonggi-do 15588South Korea Department of Mechanical engineering Korea Polytechnic UniversitySangidaehak-roSiheungGyeonggi-do 15073South Korea Medical Device Development Center Daegu-Gyeongbuk Medical Innovation Foundation(DGMIF)80Cheombok-roDong-guDaegu 41061South Korea Laboratory Animal Center Daegu-Gyeongbuk Medical Innovation Foundation(DGMIF)80 Cheombok-roDong-guDaegu 41061South Korea Department of Plastic and Reconstructive Surgery Dongsan Medical CenterKeimyung University College of Medicine1035 Dalgubeol-daeroDalseo-guDaegu 42601South Korea Institute of Pharmaceutical Science and Technology College of PharmacyHanyang University55 Hanyangdaehak-RoAnsanGyeonggi-Do 15588South Korea

Among many biomaterials,gelatin methacrylate(GelMA),a photocurable protein,has been widely used in 3D bioprinting process owing to its excellent cellular responses,biocompatibility and ***,GelMA still shows a low processability due to the severe temperature dependence of *** overcome this obstacle,we propose a two-stage temperature control system to effectively control the viscosity of *** optimize the process conditions,we evaluated the temperature of the cooling system(jacket and stage).Using the established system,three GelMA scaffolds were fabricated in which different concentrations(0,3 and 10 wt%)of silanated silica particles were *** evaluate the performances of the prepared scaffolds suitable for hard tissue regeneration,we analyzed the physical(viscoelasticity,surface roughness,compressive modulus and wettability)and biological(human mesenchymal stem cells growth,western blotting and osteogenic differentiation)***,the composite scaffold with greater silica contents(10 wt%)showed enhanced physical and biological performances inc.uding mechanical strength,cell initial attachment,cell proliferation and osteogenic differentiation compared with those of the *** results indicate that the GelMA/silanated silica composite scaffold can be potentially used for hard tissue regeneration.

关键词： 3D bioprinting gelatin methacrylate silanated silica cooling system human mesenchymal stem cells

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biological Resources within the Human Body Can be Used to Operate Neural Implants

Biological Resources within the Human Body Can be Used to Op...

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International Conference on Neural Interface and Control

作者： Daliang Leon Li Paul A. Roche Brian L. Wessel Robert J. Sclabassi Laboratory for Computational Neuroscience Departments of Neurosurgery Bioengineering and Electrical Engineering University of Pittsburgh Pittsburgh PA 15260

Implantable neural devices have many therapeutic, diagnostic, and prosthetic applications. Although there have been exciting developments in constructing these devices, two critical problems, data communication between the implanted device and external computers as well as electrical power to the device, have not yet been solved. We investigate these problems using the volume conduction properties of the human body. A prototype implantable device is constructed equipped with a volume conduction data communication channel. A new power delivery antenna is conceptualized, inspired by a study of the power delivery mechanisms of electric fish. Our investigation indicates that the volume conduction resources within the human body may provide a powerful solution to both problems.

关键词： Antenna Design data communication finite element simulation neural engineering neural implants power delivery telemetry volume conduction

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Habitat protection for sensitive species: Balancing species requirements and human constraints using bioindicators as examples

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Natural Science 2013年第5期5卷 50-62页

作者： Joanna Burger Michael Gochfeld Charles W. Powers Lawrence Niles Robert Zappalorti Jeremy Feinberg James Clarke Conserve Wildlife Foundation of New Jersey Greenwich NJ USA Consortium for Risk Evaluation with Stakeholder Participation Vanderbilt University Nashville TN USA Department of Civil and Environmental engineering Vanderbilt University Nashville TN USA Division of Life Sciences Rutgers University Piscataway NJ USA Ecology and Evolution Graduate Program Rutgers University New Brunswick NJ USA Division of Life Sciences Rutgers University Piscataway NJ USA Environmental and Occupational Health Sciences Institute Rutgers University Piscataway NJ USA Consortium for Risk Evaluation with Stakeholder Participation Vanderbilt University Nashville TN USA Environmental and Occupational Health Sciences Institute Rutgers University Piscataway NJ USA Consortium for Risk Evaluation with Stakeholder Participation Vanderbilt University Nashville TN USA Environmental and Occupational Medicine UMDNJ-Robert Wood Johnson Medical School Piscataway NJ USA Herpetological Associates inc. Jackson NJ USA

Vertebrates have particular habitat needs as a function of life cycle and reproductive stage. This paper uses four species as examples to illustrate a paradigm of environmental assessment that inc.udes physical, biological, toxicological and human dimensions. Species used inc.ude Chinook salmon (Oncorhynchus tshawytscha), northern leopard frog (Rana pipiens), northern pine snake (Pituophis m. melanoleucus), and red knot (Calidris canutus rufa, a sandpiper). The life cycles of these species inc.ude reliance on habitats that are aquatic, terrestrial, aerial, or combinations of these. Two species (frog, snake) are sedentary and two (salmon, sandpiper) are long-distance migrants. While some measurement endpoints are similar for all species (reproductive success, longevity, contaminant loads), others vary depending upon life cycle and habitat. Salmon have a restricted breeding habitat requiring coarse sand, moderate current, and high oxygen levels for adequate egg inc.bation. Leopard frogs require still water of appropriate temperature for development of eggs. Pine snakes require sand compaction sufficient to sustain a nest burrow without collapsing, and full sun penetration to the sand to allow their eggs in underground nests to inc.bate and hatch. Red knots migrate to high Arctic tundra, but inc.bate their own eggs, so temperature is less of a constraint, but feedinging habitat is. These habitat differences suggest the measurement endpoints that are essential to assess habitat suitability and to manage habitats to achieve stable and sustainable populations. Habitat use and population stability have implications for human activities for some, but not all species. Salmon are important economically, recreationally, and as part of Native American culture and diet. Red knots are of interest to people mainly because of their long, intercontinental migrations and declining populations. Other measurement endpoints for these four species illustrate the differences and similarit

关键词： Assessment Environmental Monitoring Bioindicators Habitat vulnerabilities Salmon Frog Snake Bird

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