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A novel live attenuated vaccine candidate protects chickens against subtype B avian metapneumovirus

Journal of Integrative Agriculture 2024年第5期23卷 1658-1670页

作者： Lingzhai Meng Mengmeng Yu Suyan Wang Yuntong Chen Yuanling Bao Peng Liu Xiaoyan Feng Tana He Ru Guo Tao Zhang Mingxue Hu Changjun Liu Xiaole Qi Kai Li Li Gao Yanping Zhang Hongyu Cui Yulong Gao Avian Immunosuppressive diseases Division State Key Laboratory for Animal Disease Control and PreventionHarbin Veterinary Research InstituteChineseAcademy of Agricultural SciencesHarbin150069China Avian ImmunosuppressiveDiseases DivisionStateKey LaboratoryforAnimal Disease Cont National Poultry laboratory animal resource Center Harbin 150069China

Avian metapneumovirus(aMPV) is a highly contagious pathogen that causes acute upper respiratory tract diseases in chickens and turkeys, resulting in serious economic losses. Subtype B aMPV has recently become the dominant epidemic strain in China. We developed an attenuated aMPV subtype B strain by serial passaging in Vero cells and evaluated its safety and efficacy as a vaccine candidate. The safety test showed that after the 30th passage, the LN16-A strain was fully attenuated, as clinical signs of infection and histological lesions were absent after *** LN16-A strain did not revert to a virulent strain after five serial passages in chickens. The genomic sequence of LN16-A differed from that of the parent wild-type LN16(wtLN16) strain and had nine amino acid mutations. In chickens, a single immunization with LN16-A induced robust humoral and cellular immune responses, including the abundant production of neutralizing antibodies, CD4^(+) T lymphocytes, and the Th1(IFN-γ) and Th2(IL-4 and IL-6)cytokines. We also confirmed that LN16-A provided 100% protection against subtype B aMPV and significantly reduced viral shedding and turbinate inflammation. Our findings suggest that the LN16-A strain is a promising live attenuated vaccine candidate that can prevent infection with subtype B aMPV.

关键词： avian metapneumovirus attenuated protection vaccine candidate chickens

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Inactivated H9N2 vaccines developed with early strains do not protect against recent H9N2 viruses:Call for a change in H9N2 control policy

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Journal of Integrative Agriculture 2024年第6期23卷 2144-2148页

作者： Yanjing Liu Qingqing Yu Xiangyu Zhou Wenxin Li Xinwen He Yan Wang Guohua Deng Jianzhong Shi Guobin Tian Xianying Zeng Hualan Chen College of Veterinary Medicine Gansu Agricultural UniversityLanzhou 730070China State key laboratory for animal disease Control and prevention Key Laboratory for Prevention and Control of Avian Influenza and Other Major Poultry DiseasesMinistry of Agriculture and Rural Affairs/Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150009China National Poultry laboratory animal resource Center Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150009China Harbin Weike Biotechnology Co. Ltd.Harbin 150009China

H9N2 virus has been widely distributed in wild birds and poultry around the world since its first emergence in the United States of America in 1966(Gu et al.2017;Carnaccini and Perez 2020).The virus appeared in chickens in China in the early 1990s,and over the last two decades has gradually become the dominant epidemic subtype(Sun and Liu 2015;Bi et al.2020).Although H9N2 virus infection alone cannot cause severe disease or death in poultry,H9N2 virus-infected birds experience a degree of egg production drop and can be easily infected by other pathogens,thus causing economic losses for poultry industry.

关键词： H9N2 poultry policy

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Social experience promotes the exploratory behaviour in Java Sparrows

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Avian Research 2023年第1期14卷 33-37页

作者： Qiuyang Chen Guang Chen Mengyao Shao Yuanxiu Wu Jiayu Wang Dongmei Wan Jiangxia Yin The key laboratory of animal resource and epidemic disease prevention in Liaoning Province School of Life SciencesLiaoning UniversityShenyang110036China

Personality widely exists in diverse animal *** inter-individual variance in behaviour is supposed to be influenced by social ***,it remains unknown whether the experience of social life has any carryover effects on the subsequent expression of ***,we examined exploratory behaviour in caged Java Sparrows(Lonchura oryzivora)using exploration *** were assigned to live in either a solitary or a social context for four *** compared the expressions of exploration before and after the treatments,and found that birds showed higher exploration tendencies after than before social life,while the isolated birds were consistent in their exploratory *** living experience led to differences in the exploration activities for birds without significant differences in exploration *** results indicate that social experience can make birds more proactive.

关键词： animal behaviour animal personality Exploratory behaviour Lonchura oryztivora Social experience

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Egg-spot matching in common cuckoo parasitism of the oriental reed warbler: effects of host nest availability and egg rejection

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Chinese Birds 2016年第4期7卷 199-209页

作者： Donglai Li Yanan Ruan Ying Wang Alan K.Chang Dongmei Wan Zhengwang Zhang provincial key laboratory of animal resource and epidemic disease prevention College of Life Sciences Liaoning University Ministry of Education key laboratory for Biodiversity Science and Ecological Engineering College of Life Sciences Beijing Normal University

Background: The success of cuckoo parasitism is thought to depend largely on the extent of egg matching between cuckoo and host eggs, since poor-matching cuckoo egg would lead to more frequent egg rejection by the host. In this study, we investigated how egg-spot matching between the Common Cuckoo(Cuculus canorus) and its host, the Oriental Reed Warbler(Acrocephalus orientalis) is affected by the local parasitism rate, nest availability in breeding synchronization and egg ***: We used the paired design of parasitized and their nearest non-parasitized nests where breeding occurred simultaneously to compare egg-spot matching. The image analysis was used to compare four eggshell pattern variables, namely spot size, density, coverage on the different areas of egg surface, and the distribution on the whole egg surface. Egg recognition experiments were conducted to test the effect of egg spots on egg rejection by the ***: Our results show that much better matching in almost all spot parameters tested on the side of the egg and the spot distribution on the whole egg occurred in parasitized nests than in non-parasitized nests. Matching of spot density between cuckoo and host eggs in parasitized nests increased with the synchronization between temporal availability of nests and the egg-laying period of female cuckoos. Egg recognition experiments in which the warbler eggs were deliberately painted with extra spots led to a significantly higher egg rejection rate(78.3%) than of unpainted ***: Our data suggest that both the high temporal encounter rate between cuckoo and warbler nests as well as the high egg rejection ability of the host are important factors for egg-spot matching of the cuckoos.

关键词： Brood parasite Cuckoo Egg mimicry Egg matching Spot pattern Egg rejection Parasitism rate Oriental Reed Warbler

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Identification of vocal individuality in male cuckoos using different analytical techniques

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Avian Research 2017年第3期8卷 134-140页

作者： Yang Li Canwei Xia Huw Lloyd Donglai Li Yanyun Zhang Ministry of Education key laboratory for Biodiversity and Ecological Engineering College of Life Sciences Beijing Normal University School of Science and Environment Manchester Metropolitan University provincial key laboratory of animal resource and epidemic disease prevention College of Life Sciences Liaoning University

Background: Individuality in vocalizations may provide an effective tool for surveying populations of the Common Cuckoo(Cuculus canorus) but there remains few data on which technique to use to identify individuality. In this research, we compared the within-and between-individual variation in cuckoo calls using two different analytical methods, and discuss the feasibility of using call individuality to count male cuckoos within a ***: We recorded vocalization from 13 males, and measured 15 spectro-temporal variables for each call. The majority of these call variables(n = 12) have greater variation between individuals than within individual. We first calculated the similarity(Pearson's R) for each paired calls in order to find a threshold that could distinguish calls emitted from the same or different males, and then counted the number of males based on this distinction. Second, we used the more widely accepted technique of discriminant function analysis(DFA) to identify individual male cuckoos, and compared the correct rate of classifying individuals between the two analytical ***: Similarity of paired calls from the same male was significantly higher than from different males. Under a relatively broad threshold interval, we achieved a high(>90%) correct rate to distinguish calls and an accurate estimate of male numbers. Based on banded males(n = 3), we found the similarity of paired calls from different days was lower when compared with paired calls from the same day, but this change did not obscure individual identification, as similarity values of paired calls from different days were still larger than the threshold used to distinguish calls from the same or different males. DFA also yielded a high rate(91.9%) of correct classification of ***: Our study suggests that identifying individual vocalizations can form the basis of an appropriate survey method for counting male cuckoos within a population, provided the perf

关键词： Vocal individuality Avian acoustics Common Cuckoo Correlation analysis Discriminant function analysis

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Base editors:development and applications in biomedicine

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Frontiers of Medicine 2023年第3期17卷 359-387页

作者： Yanhui Liang Fangbing Chen Kepin Wang Liangxue Lai China-New Zealand Joint laboratory on Biomedicine and Health CAS Key Laboratory of Regenerative BiologyGuangdong Provincial Key Laboratory of Stem Cell and Regenerative MedicineCentre for Regenerative Medicine and HealthHong Kong Institute of Science and InnovationGuangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou 510530China Research Unit of Generation of Large animal disease Models Chinese Academy of Medical Sciences(2019RU015)Guangzhou 510530China Sanya Institute of Swine resource Hainan Provincial Research Centre of Laboratory AnimalsSanya 572000China Guangdong provincial key laboratory of Large animal Models for Biomedicine Wuyi UniversityJiangmen 529020China

Base editor(BE)is a gene-editing tool developed by combining the CRISPR/Cas system with an individual deaminase,enabling precise single-base substitution in DNA or RNA without generating a DNA double-strand break(DSB)or requiring donor DNA templates in living *** editors offer more precise and secure genome-editing effects than other conventional artificial nuclease systems,such as CRISPR/Cas9,as the DSB induced by Cas9 will cause severe damage to the ***,base editors have important applications in the field of biomedicine,including gene function investigation,directed protein evolution,genetic lineage tracing,disease modeling,and gene *** the development of the two main base editors,cytosine base editors(CBEs)and adenine base editors(ABEs),scientists have developed more than 100 optimized base editors with improved editing efficiency,precision,specificity,targeting scope,and capacity to be delivered in vivo,greatly enhancing their application potential in ***,we review the recent development of base editors,summarize their applications in the biomedical field,and discuss future perspectives and challenges for therapeutic applications.

关键词： base editing CBE ABE ADAR DdCBE disease model therapeutic application

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Epidemiological Characteristics of Human Anthrax-China,2018-2021

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China CDC weekly 2022年第35期4卷 783-787页

作者： Huijuan Zhang Enmin Zhang Mu Guo Jinrong He Wei Li Jianchun Wei National Institute for Communicable disease Control and prevention Chinese Center for Disease Control and PreventionBeijingChina State key laboratory of Infectious disease prevention and Control BeijingChina Yunnan Institute for Endemic disease Control and prevention Dali CityYunnan ProvinceChina

Introduction:The epidemic of human anthrax is at a low level in China in recent years,but the reported incidence increased in *** order to understand the current landscape of research and knowledge about anthrax in China,the epidemiological characteristics of anthrax in humans from 2018 to 2021 were analyzed and the prevention and control suggestions were ***:Surveillance data of anthrax in humans and livestock,together with human outbreaks data during 2018-2021,were collected and analyzed by descriptive statistics *** number and proportion of outbreaks,cases and deaths by provincial-level administrative divisions(PLADs),clinical types,and contributing factors were ***:A total of 1,244 cases of human anthrax and 53 outbreaks were reported from 2018 to 2021 in *** the incidence of anthrax declined from 2018 to 2020,it increased in *** regions of anthrax were mainly located in the west and the northeast PLADs of China,though cases were reported in some central and eastern PLADs in *** and middle-aged men involved in animal husbandry were found to be at a higher risk of *** the reported outbreaks were associated with the exposure of infected livestock.A total of 296 livestock anthrax cases were ***:The increased incidence and wider geographical distribution of human anthrax in 2021 were found to be the result of inadequate supervision of diseased animals as well as updated diagnostic *** such,the monitoring of risk factors and emergency preparation procedures should be strengthened at the national *** addition,it is also critical to strengthen health education for high-risk occupational groups and strengthen professional training for local ***,more measures should be carried out to strengthen anthrax surveillance in livestock husbandry.

关键词： prevention husbandry strengthened

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Loop-Mediated Isothermal Amplification Combined with Lateral Flow Dipstick for On-Site Diagnosis of African Swine Fever Virus

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Virologica Sinica 2021年第2期36卷 325-328页

作者： Lei Zuo Zengxu Song Yi Zhang Xiwen Zhai Yaru Zhai Xueran Mei Xin Yang Hongning Wang College of Life Sciences Sichuan UniversityAnimal Disease Prevention and Food Safety Key Laboratory of Sichuan ProvinceKey Laboratory of Bio-Resource and EcoEnvironment of Ministry of EducationChengdu 610064China Sichuan provincial Center for animal disease Control and prevention Chengdu 610064China

Dear Editor,African swine fever (ASF) is one of the most important porcine infectious diseases and has caused huge economic losses worldwide (Teklue et al. 2020). African swine fever virus (ASFV), the causative agent of ASF, is a double stranded DNA virus with a genome about 170 to 194 kb in length, and encodes 150 to 167 open reading frames(ORFs). There are more than 50 proteins involved in the mature virions. Among these proteins, p72 encoded by B646L gene is the essential component in the formation of ASFV particles. The sequence of B646L gene is also usually used for the genotyping of ASFV, and there are 24B646L genotypes (Quembo et al. 2018).

关键词： Swine swine African

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Screening of Proteins Interacting with Nonstructural 1 Protein of H5N1 Avian Influenza Virus from T7-phage Display Library

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Chemical Research in Chinese Universities 2012年第1期28卷 103-107页

作者： ZHU Chun-yu1,2, SUN Ting-ting2, ZHAO Jian1,2, WANG Ning1,2, ZHENG Fang-liang1, AI Hai-xin1, ZHU Jun-feng1,2, WANG Xiao-ying3, ZHU Ying4, WU Jian-guo4 and LIU Hong-sheng1,2 1. key laboratory of animal resource and epidemic disease prevention of Liaoning Province, 2. Research Center for Computer Simulating and Information Processing of Bio-macromolecules of Liaoning Province, Academy of Science, Liaoning University, Shenyang 110036, P. R. China 3. Institute of Nutrition and Food Safety, Chinese Center for disease Control and prevention, Beijing 100050, P. R. China 4. State key laboratory of Virology, Wuhan University, Wuhan 430072, P. R. China Research Center for Computer Simulating and Information Processing of Bio-macromolecules of Liaoning Province Academy of Science Liaoning University Key Laboratory of Animal Resource and Epidemic Disease Prevention of Liaoning Province Shenyang 110036 Research Center for Computer Simulating and Information Processing of Bio-macromolecules of Liaoning Province Academy of Science Liaoning University Shenyang 110036 Liaoning University Key Laboratory of Animal Resource and Epidemic Disease Prevention of Liaoning Province Shenyang 110036 Institute of Nutrition and Food Safety Chinese Center for Disease Control and Prevention Beijing 100050 Wuhan University State Key Laboratory of Virology Wuhan 430072

Avian influenza virus（AIV） nonstructural 1（NS1） gene was amplified by real-time polymerse chain reac tion（RT-PCR） and inserted into pET28a, then transformed into E. coli BL21（DE3） competent cell. With the induction of isopropyl-β-D-thiogalactoside（IPTG） and the purification of Ni-NTA column, we finally obtained purified NS1 protein. T7-phage display system was used to screen the proteins that interacted with NS1 from lung cell cDNA li brary. The selected positive clones were identified by DNA sequencing and analyzed by BLAST program in Gene Bank. Two proteins were obtained as NS1 binding proteins, Homo sapiens nucleolar and coiled-body phosphoprotein 1（NOLC1） and Homo sapiens similar to colon cancer-associated antigen. By co-immunoprecipitation and other me thods, Homo sapiens NOLC1 was found to interact with the NS1 protein, the results would provide the basis for fur ther studying biological function of NS1 protein.

关键词： T7-phage display Nonstructural 1 (NS 1) protein Interacting protein

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Double knock-in pig models with elements of binary Tet-On and phiC31 integrase systems for controllable and switchable gene expression

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Science China(Life Sciences) 2022年第11期65卷 2269-2286页

作者： Qin Jin Xiaoyu Yang Shixue Gou Xiaoyi Liu Zhenpeng Zhuang Yanhui Liang Hui Shi Jiayuan Huang Han Wu Yu Zhao Zhen Ouyang Quanjun Zhang Zhaoming Liu Fangbing Chen Weikai Ge Jingke Xie Nan Li Chengdan Lai Xiaozhu Zhao Jiaowei Wang Meng Lian Lei Li Longquan Quan Yinghua Ye Liangxue Lai Kepin Wang CAS key laboratory of Regenerative Biology Joint School of Life SciencesGuangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou Medical UniversityGuangzhou 510530China Guangdong provincial key laboratory of Stem Cell and Regenerative Medicine Centre for Regenerative Medicine and HealthHong Kong Institute of Science and InnovationGuangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou 510530China Sanya institute of Swine resource Guangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesHainan Provincial Research Center of Laboratory AnimalsSanya 572000China Research Unit of Generation of Large animal disease Models Chinese Academy of Medical Sciences(2019RU015)Guangzhou 510530China University of Chinese Academy of Sciences Beijing 100049China Bioland laboratory(Guangzhou Regenerative Medicine and Health Guangdong laboratory) Guangzhou 510005China Guangdong provincial key laboratory of laboratory animals Guangdong Laboratory Animals Monitoring InstituteGuangzhou 510633China Guangdong provincial key laboratory of Large animal models for Biomedicine Wuyi UniversityJiangmen 529020China

Inducible expression systems are indispensable for precise regulation and in-depth analysis of biological *** Tet-On system has been widely employed to regulate transgenic expression by *** pig models with tetracycline regulatory elements were generated through random *** process often resulted in uncertain expression and unpredictable phenotypes,thus hindering their ***,by precise knock-in of binary Tet-On 3G elements into Rosa26 and Hipp11 locus,respectively,a double knock-in reporter pig model was *** characterized excellent properties of this system for controllable transgenic expression both in vitro and in *** att P sites were arranged to flank the td Tomato to switch reporter *** or multiple gene replacement was efficiently and faithfully achieved in fetal fibroblasts and nuclear transfer *** display the flexible application of this system,we generated a pig strain with Dox-inducing h KRASexpression through phiC31 integrase-mediated cassette *** eight months of Dox administration,squamous cell carcinoma developed in the nose,mouth,and scrotum,which indicated this pig strain could serve as an ideal large animal model to study ***,the established pig models with controllable and switchable transgene expression system will provide a facilitating platform for transgenic and biomedical research.

关键词： double knock-in Tet-On system phiC31-mediated cassette exchange pig model hKRASG12D-related tumorigenesis

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