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Assessment of the binding interactions of SARS-CoV-2 spike glycoprotein variants

Journal of Pharmaceutical Analysis 2022年第1期12卷 58-64页

作者： Deepa Raghu Pamela Hamill Arpitha Banaji Amy McLaren Yu-Ting Hsu product characterization MilliporeSigmaRockvilleMD20850USA product characterization MilliporeSigmaStirlingFK94NFUK Process and Analytical Development MilliporeSigmaSt.LouisMO63118USA

Severe acute respiratory syndrome-associated coronavirus 2 is a major global health issue and is driving the need for new *** surface spike protein,which plays a central role in virus infection,is currently the target for vaccines and neutralizing *** emergence of novel variants with multiple mutations in the spike protein may reduce the effectiveness of neutralizing antibodies by altering the binding activity of the protein with angiotensin-converting enzyme 2(ACE2).To understand the impact of spike protein mutations on the binding interactions required for virus infection and the effectiveness of neutralizing monoclonal antibody(mAb)therapies,the binding activities of the original spike protein receptor binding domain(RBD)sequence and the reported spike protein variants were investigated using surface plasmon *** addition,the interactions of the ACE2 receptor,an antispike mAb(mAb1),a neutralizing mAb(mAb2),the original spike RBD sequence,and mutants D614G,N501Y,N439K,Y453F,and E484K were *** to the original RBD,the Y453F and N501Y mutants displayed a significant increase in ACE2 binding affinity,whereas D614G had a substantial reduction in binding *** mAb-RBD mutant proteins displayed a reduction in binding affinities relative to the original RBD,except for the E484K-mAb1 *** potential neutralizing capability of mAb1 and mAb2 was ***,mAb1 failed to inhibit the ACE2-RBD interaction while mAb2 inhibited the ACE2-RBD interactions for all RBD mutants,except mutant E484K,which only displayed partial blocking.

关键词： Surface plasmon resonance SARS-CoV-2 Monoclonal antibody RBD ACE2

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Development of a physiologically relevant dripping analytical method using simulated nasal mucus for nasal spray formulation analysis

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Journal of Pharmaceutical Analysis 2016年第5期6卷 283-291页

作者： Tina Masiuk Parul Kadakia Zhenyu Wang Respiratory product Development Merck Research Laboratories Merck & Co.Inc.

Current methods for nasal spray formulations have been elementary evaluating the dripping characteristics of a formulation and have not assessed the behavior of the nasal formulation in the presence of varying types of mucus depending on the indication or diseased state. This research investigated the effects of nasal mucus on the dripping behavior of nasal formulations and focused on developing an improved in vitro analytical test method that is more physiologically relevant in characterizing nasal formulation dripping behavior. Method development was performed using simulated nasal mucus preparations for both healthy and diseased states as coatings for the dripping experiment representing a wide range of viscosity. Factors evaluated during development of this in vitro test method included amount of mucus, application of mucus, drying times, and compatibility of the mucus on a C18 Thin Layer Chromatography(TLC) substrate. The dripping behavior of nasal formulations containing a range of 1%Avicel to 3.5% Avicel was assessed by actuating the nasal spray on a perpendicular TLC plate coated with either healthy or diseased simulated nasal mucus. After actuation of the nasal spray, the dripping of the formulation on the coated TLC plate was measured after the plate was repositioned vertically. The method that was developed generated reproducible results on the dripping behavior of nasal formulations and provided critical information about the compatibility of the formulation with the nasal mucus for different diseased states, aiding in nasal spray formulation development and physical characterization of the nasal spray.

关键词： Nasal spray Nasal spray dripping method Nasal mucus Nasal formulation dripping

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Identification and characterization of Pichia membranifaciens Hmp-1 isolated from spoilage blackberry wine

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Journal of Integrative Agriculture 2018年第9期17卷 2126-2136页

作者： WANG Ying ZHAO Yan-cun FAN Lin-lin XIA Xiu-dong LI Ya-hui ZHOU Jian-zhong Institute of Agro-product Processing Jiangsu Academy of Agricultural Sciences Institute of Plant Protection Jiangsu Academy of Agricultural Sciences

The pellicle-forming yeast could cause the quality deterioration of wine. In this study, a pellicle-forming strain Hmp-1 was isolated from the spoilage blackberry wine, and identified as Pichia membranifaciens based on the morphology and partial nucleotide sequence of 26S rDNA. The effects of fermentation conditions （ethanol, sulfur dioxide, sugar, and temperature） on the growth of P. membranifaciens strain Hmp-1 and Saccharomyces cerevisiae strain FM^S-115 （a strain used for the blackberry wine fermentation） were investigated, respectively. The results indicated that Hmp-1 had lower resistance to these factors compared to FM-S-115, and the growth of Hmp-1 was completely inhibited by 10% （v/v） or 50 mg L-~ SO2 during the fermentation of blackberry wine. These results suggested that Hmp-1 could effectively be controlled by increasing ethanol or SO2 concentration during the fermentation and storage of blackberry wine. Furthermore, the analysis based on gas chromatography-mass spectrometry （GC-MS） showed that Hmp-1 remarkably decreased kinds of volatile compounds in blackberry wine, especially aldehydes and esters. In addition, some poisonous compounds were detected in the blackberry wine fermented by FM-S-115 and Hmp-l. These results suggested that Hmp-1 was a major cause leading to the quality deterioration of blackberry wine.

关键词： Picihia membranifaciens Hmp-1 blackberry wine pellicle-forming spoilage population dynamics volatile compounds

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Preparation and characterization of Natural Rubber/Silica Nanocomposites using Rule of Similarity in Latex

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Journal of Wuhan University of Technology(Materials Science) 2013年第5期28卷 997-1002页

作者：罗勇悦 FENG Chunfang WANG Qinghuang YI Zhifeng QIU Quanfang Kong LX 彭政 Key Laboratory of Tropical Crop product Processing Chinese Agricultural MinistryAgricultural Product Processing Research InstituteChinese Academy of Tropical Agricultural Sciences Centre for Material and Fiber Innovation Institute for Technology Research InnovationDeakin University State Engineering and Technology Research Center for Key Tropical Crops

Rule of similarity and latex compounding techniques were combined for the first time to prepare natural rubber/nanosilica （NR/SiO2） nanocomposite with core-shell nanosilica-poly （methyl methacrylate）（SiO2-PMMA） particles and PMMA-modified natural rubber matrix （NR-PMMA）. The micro- structure of SiO2 and nanocomposites with different SiO2 contents was characterized by fourier transform infrared spectroscopy （FTIR）; the morphology of nanocomposites was investigated with scanning electron microscopy （SEM）; the tensile strength was characterized by tensile testing machine and the thermal stability of composites was studied by thermal gravimetric analysis. Results showed that PMMA chains have successfully grafted onto the surface of SiO2, and the core-shell SiO2-PMMA nanoparticles and NR-PMMA latex have been perfectly incorporated. SiO2-PMMA nanoparticles are evenly distributed over the NR matrix with an average size in the range of 60-100 nm at the low content （SiO2≤ 3 wt%）, while aggregations are apparently observed when 5 wt% SiO2 is loaded. In addition, NP/SiO2 composities possess a considerable improvement in ageing resistance compared with the pure NR. The tensile strength of composite increases from 6.99 to 12.72 MPa, reaching the highest value at a 0.5 wt% SiO2 loading, and then the figure decreases gradually because of the aggregation of SiO2 nanoparticles. It is anticipated that the reported process is to provide a simple and economic way for preparing NR composites.

关键词： natural rubber silica nanocomposite latex compounding rule of similarity

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Distribution of nitrogen and oxygen compounds in shale oil distillates and their catalytic cracking performance

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Petroleum Science 2020年第6期17卷 1764-1778页

作者： Xiao-Bo Chen Xin-Yang Zhang Ru-Meng Qin Sheng-Jie Shan Pan-Deng Xia Nan Li Jun Pu Ji-Xia Liu Yi-Bin Liu Chao-He Yang State Key Laboratory of Heavy Oil Processing China University of PetroleumQingdao 266580China Shandong Institute for product Quality Inspection Jinan 250102China

The positive-and negative-ion electrospray ionization(ESI)coupled with Fourier transform-ion cyclotron resonance mass spectrometry(FT-ICR MS)was employed to identify the chemical composition of heteroatomic compounds in four distillates of Fushun shale oil,and their catalytic cracking performance was *** are nine classes of basic nitrogen compounds(BNCs)and eleven classes of non-basic heteroatomic compounds(NBHCs)in the different *** dominant BNCs are mainly basic N1 class *** dominant NBHCs are mainly acidic O2 and O1 class species in the300-350℃,350-400℃,and 400-450℃distillates,while the neutral N1,N1 O1 and N2 compounds become relatively abundant in the>450℃*** basic N1 compounds and acidic O1 and O2 compounds are separated into different distillates by the degree of alkylation(different carbon number)but not by aromaticity(different double-bond equivalent values).The basic N1 O1 and N2 class species and neutral N1 and N2 class species are separated into different distillates by the degrees of both alkylation and *** the catalytic cracking of Fushun shale oil,the classes of BNCs in the liquid products remain unchanged,while the classes and relative abundances of NBHCs vary significantly.

关键词： Molecular characterization Shale oil ESI FT-ICR MS Nitrogen-and oxygen-containing compounds Catalytic cracking performance

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Detection and quantitation of host cell proteins in monoclonal antibody drug products using automated sample preparation and data-independent acquisition LC-MS/MS

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Journal of Pharmaceutical Analysis 2021年第6期11卷 726-731页

作者： Lisa Strasser Giorgio Oliviero Craig Jakes Izabela Zaborowska Patrick Floris Meire Ribeiro da Silva Florian Füssl Sara Carillo Jonathan Bones characterization and Comparability Laboratory NIBRT-National Institute for Bioprocessing Research and TrainingDublinA94 X099Ireland School of Chemical and Bioprocess Engineering University College DublinDublinD04 V1W8Ireland

Ensuring the removal of host cell proteins(HCPs) during downstream processing of recombinant proteins such as monoclonal antibodies(m Abs) remains a *** residual HCPs might affect product stability or safety,constant monitoring is required to demonstrate their removal to be below the regulatory accepted level of 100 ng/*** current standard analytical approach for this procedure is based on ELISA;however,this approach only measures the overall HCP ***,the use of orthogonal methods,such as liquid chromatography-mass spectrometry(LC-MS),has been established,as it facilitates the quantitation of total HCPs as well as the identification and quantitation of the individual HCPs *** the present study,a workflow for HCP detection and quantitation using an automated magnetic bead-based sample preparation,in combination with a data-independent acquisition(DIA) LC-MS analysis,was *** the same instrumental setup commonly used for peptide mapping analysis of m Abs allows for its quick and easy implementation into pre-existing workflows,avoiding the need for dedicated instrumentation or ***,quantitation of HCPs over a broad dynamic range was enabled to allow monitoring of problematic HCPs or to track changes upon altered bioprocessing conditions.

关键词： Data-independent acquisition Host cell proteins Critical quality attributes Liquid chromatography-mass spectrometry Monoclonal antibody Chinese hamster ovary cells

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Interaction Between Ustilaginoidea virens and Rice and Its Sustainable Control

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Rice science 2024年第3期31卷 269-284页

作者： ZHANG Fengmin CAO Zhenzhen ZHENG Xin HE Yuntao CHEN Mingxue LIN Xiaoyan Rice product Quality Inspection&Supervision Testing Center of Ministry of Agriculture and Rural Affairs China National Rice Research InstituteHangzhou 310006China

Ustilaginoidea virens is a common rice pathogen that can easily lead to a decline in rice quality and the production of toxins pose potential risks to human *** this review,we present a comprehensive literature review of research since the discovery of rice false *** provide a comprehensive and,at times,critical overview of the main results and findings from related research,and propose future research ***,we delve into the interaction between *** and rice,including the regulation of transcription factors,the process of *** infecting rice panicles,and the plant immune response caused by rice *** that,we discuss the identification and characterization of mycotoxins produced by the pathogenic fungus,as well as strategies for disease *** emphasize the importance of comprehensive agricultural prevention and control methods for the sustainable management of *** knowledge will update our understanding of the interaction between *** and rice plants,offering a valuable perspective for those interested in ***.

关键词： rice false smut flower-specific infection mycotoxin food safety plant disease

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Multiarm Star Poly(ε-caprolactone) with Hyperbranched Polyamidoamine as Core Capable of Selective Accommodating Cationic or Anionic Guests

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Chinese Journal of Polymer Science 2015年第6期33卷 920-930页

作者： Chen Zhan Si Li Jin Cui 陈宇 Department of Chemistry School of SciencesTianjin Universityand Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) National Foodstuff Inspection Center Tianjin Product Quality Inspection Technology Research Institute

Hyperbranched polyamidoamines （HPAs） were directly employed as macroinitiators to initiate the Sn（Oct）2 catalyzed ring-opening polymerization of ε-caprolactone （CL）, resulting in multiarm star copolymers with poly（6- caprolactone）（PCL） as shells and HPA as core （HPA-b-PCL）. From 1H-NMR characterization it was deduced that both the primary amines and the secondary amide groups of HPAs could initiate the CL polymerization, and the initiation efficiency increased when more CL monomers were fed. The average arm-numbers of the obtained stars were in the range of 115-353. Differential scanning calorimetry measurements demonstrated that the melting and crystallization temperatures, fusion and crystallization enthalpy and the degree of crystallinity of the star polymers increased as the PCL arm length increased. HPA- b-PCL stars could be used as nanocarriers to efficiently accommodate anionic dyes at acidic condition, while load cationic dyes at basic condition. Compared with the dye-loading behavior of multiarrn star PCL with the neutral hyperbranched polyglycerol as core, it was deduced that HPA-b-PCL nanocarriers accommodated anionic dyes using the HPA core, while loaded cationic dyes using both the HPA core and the PCL shell. Dynamic light scattering analyses also supported such deduction. Furthermore, HPA-b-PCL nanocarriers could selectively load the anionic Eosin Y or the cationic methylene blue from their mixture at pH = 6 or 9, respectively, realizing their separation.

关键词： Nanocarrier Hyperbranched Polyamidoamine Poly(ε-caprolactone) Star polymers.

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Synthesis and characterization of Organoboryl Germanium(Ⅱ)Oxides Containing Ge-O-B and Ge-O-B-O-Ge Cores

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Chinese Journal of Structural Chemistry 2021年第6期40卷 811-820,686页

作者： ZHANG De-Xiang LI Yao PENG Lan-Xin LI Hai-Pu YANG Ying School of Chemistry and Chemical Engineering Central South UniversityChangsha 410083China Key Laboratory of Hunan Province for Water Environment and Agriculture product Safety Changsha 410083China

Organoboryl germanium(Ⅱ)oxides were synthesized from the 1,4-addition reaction of L′Ge(L′=HC[C(CH2)N(Ar)]C(Me)N(Ar),Ar=2,6-iPr2C6H3)with selected monosubstituted arylboronic acids RB(OH)2(R=2,6-Me2C6H3,2,4,6-Me3C6H2,1-Naph)at the molar ratios of 1:1 and 2:*** mononuclear products RB(OH)OGeL(L=CH[C(Me)N(Ar)]2,Ar=2,6-iPr2C6H3;R=2,6-Me2C6H3(1),2,4,6-Me3C6H2(2),1-Naph(3))containing the Ge–O–B core were obtained smoothly through the 1:1 ***,the reaction of L′Ge with 2,6-Me2C6H3B(OH)2 in a 2:1 ratio gave only the mononuclear product(1)instead of the expected binuclear ***’s more,a new borate compound[(2,6-Me2C6H3)4B5O6]-[H:C]+(4)(:C=C[N(iPr)C(Me)]2)was concomitantly formed when the in situ prepared L′Ge was used as the *** contrast,the use of 2,4,6-Me3C6H2B(OH)2 or 1-NaphB(OH)2 as the organoboryl source in the similar reaction led to the formation and isolation of the binuclear products RB(OGeL)2(R=2,4,6-Me3C6H2(5),1-Naph(6))containing the Ge–O–B–O–Ge core in a straight *** 1~6 were determined by single-crystal X-ray diffraction analysis.

关键词： organic boronic acid germylene synthesis crystal structure

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characterization and biodegradation kinetics of a new cold-adapted carbamazepine-degrading bacterium, Pseudomonas sp. CBZ-4

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Journal of Environmental Sciences 2013年第11期25卷 2281-2290页

作者： Ang Li Rui Cai Di Cui Tian Qiu Changlong Pang Jixian Yang Fang Ma Nanqi Ren State Key Laboratory of Urban Water Resource and Environment Harbin Institute of Technology National Supervision and Inspection Center of Environmental Protection Equipment Quality Yixing Urban Supervision andInspection Administration of Product Quality

Carbamazepine is frequently detected in waters and hardly eliminated during conventional wastewater treatment processes due to its complicated chemical structure and resistance to biodegradation. A carbamazepine-degrading bacterium named CBZ-4 was isolated at a low temperature （10℃） from activated sludge in a municipal wastewater treatment plant. Strain CBZ-4, which can use carbamazepine as its sole source of carbon and energy, was identified as Pseudomonas sp. by the 16S rRNA gene sequence. The composition and percentage of fatty acids, which can reveal the cold-adaptation mechanism of strain CBZ-4, were determined. Strain CBZ-4 can effectively degrade carbamazepine at optimal conditions： pH 7.0, 10℃, 150 r/min rotation speed, and 13% inoculation volume. The average removal rate of carbamazepine was 46.6% after 144 hr of incubation. The biodegradation kinetics of carbamazepine by CBZ-4 was fitted via the Monod model. Vmax and Ks were found to be 0.0094 hr^-1 and 32.5 mg/L, respectively.

关键词： carbamazepine low temperatures biodegradation kinetics Pseudomonas sp.

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