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Mechanism of Building-Up Deposited Layer during Electro-Spark Deposition

Journal of Surface Engineered Materials and Advanced Technology 2012年第4期2卷 258-263页

作者： Amin D. Thamer Mohammed H. Hafiz Baha S. Mahdi Dean of Material Engineering Dept. Baghdad Iraq Lacturer in Production & Metallurgical Eng. Dept. Baghdad Iraq prisedent of university of tehchnology Baghdad Iraq

This paper studies the mechanism of formation of the deposit layer by (ESD) electro-spark deposition process. Inconel 738 substrates are coated with a deposited layer of NI6625 (Inconel 625). Selections of these two alloys have been done because they had wide applications and importance in the industry especially in gas turban blades in inland stations and in aircraft engines. ESD is suggested because it has a low input heat process which eliminates the effect of HAZ in these Ni-superfluous due to their sustainability to micro-cracks. The coating contains many deposited sub-layers coming from evaporated and melted micro-regions as a result of locally high heat generated by discharging a series of capacitors charged and discharged in a controlled manner between electrode and substrate material. The maximum deposition rates at the beginning of the process and decreases until been in a steady state condition due to the nature of the resultant morphology of the created surface.

关键词： ESD ESA Electro-Spark Deposition IN738L Repairing NI6625 Surface Repairing of Gas Turbine Blades

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Embryonic stem cell as nuclear donor could promote in vitro development of the heterogeneous reconstructed embryo

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Chinese Science Bulletin 2002年第21期47卷 1811-1815页

作者： WANG Hong ZHENG Ruizhen XU Ying LIAN Li AN Lijia CHEN Dayuan Laboratory of Molecular Developmental Biology Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100080China Department of Biological Engineering Dalian University of TechnologyDalian 116012China State Key Laboratory of Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing 100080China College of Animal Science and tehchnology Nanjing Agricultural UniversityNanjing 210095China

The nucleus of a somatic cell could be dedif-ferentiated and reprogrammed in an enucleated heterogeneous oocyte. Some reconstructed oocytes could develop into blastocysts in vitro, and a few could develop into term normally after transferred into foster mothers, but most of cloning embryos fail to develop to term. In order to evaluate the efficacy of embryonic stem cell as nucleus donor in interspecific animal cloning, we reconstructed enucleated rabbit oocytes with nuclei from mouse ES cells, and analyzed the developmental ability of reconstructed embryos in vitro. Two kinds of fibroblast cells were used as donor control, one derived from ear skin of an adult Kunming albino mouse, and the other derived from a mouse fetus. Three types of cells were transferred into perivitelline space under zona pellu-cida of rabbit oocytes respectively. The reconstructed oocytes were fused and activated by electric pulses, and cultured in vitro. The developmental rate of reconstructed oocytes derived from embryonic

关键词： embryonic stem cell fibroblast mouse rabbit inter-specific nuclear transfer.

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