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Identific tion of quantitative trait loci and associated candidate genes for pregnancy success in Angus–Brahman crossbred heifers

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Journal of Animal science and Biotechnology 2024年第1期15卷 162-170页

作者： Quinn A.Hoorn Gabriel A.Zayas Eduardo E.Rodriguez Laura M.Jensen Raluca G.Mateescu Peter J.Hansen Department of Animal sciences Donald Henry Barron Reproductive and Perinatal Biology Research Programand the Genetics InstituteUniversity of FloridaGainesvilleFLUSA school of Applied systems Biology La Trobe UniversityBundooraVIC 3083Australia

Background In beef cattle, more than 50% of the energy input to produce a unit of beef is consumed by the female that produced the calf. Development of genomic tools to identify females with high genetic merit for reproductive function could increase the profitability and sustainability of beef *** Genome-wide association studies(GWAs) were performed using a single-step genomic best linear unbiased prediction approach on pregnancy outcome traits from a population of Angus–Brahman crossbred heifers. Furthermore, a validation GWAs was performed using data from another farm. Heifers were genotyped with the Bovine GGP F250 array that contains 221,077 sNPs. In the discovery population, heifers were bred in winter breeding seasons involving a single round of timed artificial insemination(AI) followed by natural mating for 3 months. Two phenotypes were analyzed: pregnancy outcome to first-service AI(PAI;n = 1,481) and pregnancy status at the end of the breeding season(PEBs;n = 1,725). The heritability was estimated as 0.149 and 0.122 for PAI and PEBs, respectively. In the PAI model, one quantitative trait locus(QTL), located between 52.3 and 52.5 Mb on BTA7, explained about 3% of the genetic variation, in a region containing a cluster of γ-protocadherin genes and sLC25A2. Other QTLs explaining between 0.5% and 1% of the genetic variation were found on BTA12 and 25. In the PEBs model, a large QTL on BTA7 was synonymous with the QTL for PAI, with minor QTLs located on BTA5, 9, 10, 11, 19, and 20. The validation population for pregnancy status at the end of the breeding season were Angus–Brahman crossbred heifers bred by natural mating. In concordance with the discovery population, the large QTL on BTA7 and QTLs on BTA10 and 12 were *** In summary, QTLs and candidate sNPs identified were associated with pregnancy outcomes in beef heifers, including a large QTL associated with a group of protocadherin genes. Confirmation of these associations

关键词： Beef cattle Fertility GWAs QTL

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The resistance switching performance of the memristor improved effectively by inserting carbon quantum dots(CQDs)for digital information processing

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Nano research 2024年第9期17卷 8438-8446页

作者： Tianqi Yu Jie Li Wei Lei suhaidi shafe Mohd Nazim Mohtar Nattha Jindapetch Paphavee van Dommelen Zhiwei Zhao Joint International research Laboratory of Information Display and Visualization School of Electronic Science and EngineeringSoutheast UniversityNanjing 210096China institute of Nanoscience and Nanotechnology University Putra MalaysiaSerdangSelangor 43400Malaysia Department of Electrical Engineering Faculty of EngineeringPrince of Songkla UniversityHat YaiSongkhla 90112Thailand Division of Physical science Faculty of SciencePrince of Songkla UniversityHat Yai Campus 15 KarnjanavanichHat YaiSongkhla 90110Thailand

As an emerging information device that adapts to development of the big data era,memristor has attracted much attention due to its advantage in processing massive ***,the nucleation and growth of conductive filaments often exhibit randomness and instability,which undoubtedly leads to a wide and discrete range of switching parameters,damaging the electrical performance of *** this work,a strategy of inserting carbon quantum dots(CQDs)into graphene oxide(GO)resistance layer is utilized to improve the stability of the switching parameters and the reliability of the device is *** with GO-based devices,GO/CQDs/GO-based devices exhibit a more stable resistance switching curve,low power,lower and more concentrated threshold voltage parameters with lower variation coefficient,faster switching speed,and more stable retention and *** cause-inducing performance improvement may be attributed to the local electric field generated by CQDs in resistance switching that effectively guides the formation and rupture of conductive filaments,which optimizes the effective migration distance of Ag^(+),thereby improving the uniformity of resistance ***,a convolutional neural network model is constructed to identify the CIFAR-10 data set,showing the high recognition accuracy of online and offline *** cross-kernel structure is used to further implement convolutional image processing through multiplication and accumulation *** work provides a solution to improve the performance of memristors,which can contribute to developing digital information processing.

关键词： carbon quantum dots memristor uniformity convolutional neural network convolutional image processing

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Extracellular vesicles as tools and targets in therapy for diseases

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signal Transduction and Targeted Therapy 2024年第3期9卷 872-912页

作者： Mudasir A.Kumar sadaf K.Baba Hana Q.sadida sara Al.Marzooqi Jayakumar Jerobin Faisal H.Altemani Naseh Algehainy Mohammad A.Alanazi Abdul-Badi Abou-samra Rakesh Kumar Ammira s.Al-shabeeb Akil Muzafar A.Macha Rashid Mir Ajaz A.Bhat Watson-Crick Centre for Molecular Medicine Islamic University of Science and TechnologyAwantiporaKashmir 192122India Department of Human Genetics-Precision Medicine in Diabetes Obesity and Cancer ProgramSidra MedicineDohaQatar Qatar Metabolic institute Academic Health SystemHamad Medical CorporationDohaQatar Department of medical Laboratory Technology Prince Fahad Bin Sultan Chair for Biomedical ResearchFaculty of Applied Medical SciencesUniversity of TabukTabukSaudi Arabia school of Biotechnology Shri Mata Vaishno Devi UniversityKatraIndia

Extracellular vesicles(EVs)are nano-sized,membranous structures secreted into the extracellular *** exhibit diverse sizes,contents,and surface markers and are ubiquitously released from cells under normal and pathological *** serum is a rich source of these EVs,though their isolation from serum proteins and non-EV lipid particles poses *** vesicles transport various cellular components such as proteins,mRNAs,miRNAs,DNA,and lipids across distances,influencing numerous physiological and pathological events,including those within the tumor microenvironment(TME).Their pivotal roles in cellular communication make EVs promising candidates for therapeutic agents,drug delivery systems,and disease *** in cancer diagnostics,EV detection can pave the way for early identification and offers potential as diagnostic ***,various EV subtypes are emerging as targeted drug delivery tools,highlighting their potential clinical *** need for non-invasive biomarkers to monitor biological processes for diagnostic and therapeutic purposes remains *** into the unique composition of EVs could unlock advanced diagnostic and therapeutic avenues in the *** this review,we discuss in detail the roles of EVs across various conditions,including cancers(encompassing head and neck,lung,gastric,breast,and hepatocellular carcinoma),neurodegenerative disorders,diabetes,viral infections,autoimmune and renal diseases,emphasizing the potential advancements in molecular diagnostics and drug delivery.

关键词： diseases sized sizes

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Diagnosis,treatment and prevention of severe acute respiratory syndrome coronavirus 2 infection in children:experts'consensus statement updatedforthe Omicron variant

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World Journal of Pediatrics 2024年第3期20卷 272-286页

作者： Rong-Meng Jiang Zheng-De Xie Yi Jiang Xiao-Xia Lu Run-Ming Jin Yue-Jie Zheng Yun-Xiao shang Bao-Ping Xu Zhi-sheng Liu Gen Lu Ji-Kui Deng Guang-Hua Liu Xiao-Chuan Wang Jian-she Wang Lu-Zhao Feng Wei Liu Yi Zheng sai-Nan shu Min Lu Wan-Jun Luo Miao Liu Yu-Xia Cui Le-Ping Ye A-Dong shen Gang Liu Li-Wei Gao Li-Juan Xiong Yan Bai Li-Kai Lin Zhuang Wei Feng-Xia Xue Tian-You Wang Dong-Chi Zhao Jian-Bo shao Daniel Kwok-keung Ng Gary Wing-kin Wong Zheng-Yan Zhao Xing-Wang Li Yong-Hong Yang Kun-Ling shen Diagnosis and Treatment Center of Infectious Diseases Beijing Ditan HospitalCapital Medical UniversityBeijing 100015China Beijing Key Laboratory of Pediatric Respiratory Infection Diseases Key Laboratory of Major Diseases in ChildrenMinistry of EducationNational Clinical Research Center for Respiratory DiseasesResearch Unit of Critical Infection in ChildrenChinese Academy of Medical Sciences2019RU016Laboratory of Infection and VirologyBeijing Pediatric Research InstituteBeijing Children's HospitalCapital Medical UniversityNational Center for Children's HealthBeijing 100045China Department of Pediatrics Renmin Hospital of Wuhan UniversityWuhan 430060China Department of Respiratory Wuhan Children's HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430016China Department of Pediatrics Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Department of Respiratory Shenzhen Children's HospitalShenzhen 518038China Department of Pediatric Respiratory Shengjing Hospital Affiliated to China Medical UniversityShenyang 110004China Department of Respiratory Beijing Children's HospitalCapital Medical UniversityNational Clinical Research Center for Respiratory DiseasesNational Center for Children's HealthBeijing 100045China Department of Neurology Wuhan Children's HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430016China Department of Respiratory Guangzhou Women and Children's Medical CenterGuangzhou 510623China Department of Infectious Diseases Shenzhen Children's HospitalShenzhen 518038China Department of Pediatrics Fujian Branch of Shanghai Children's Medical CenterFujian Children's HospitalFuzhou350005China Department of Clinical Immunology and Allergy Children's Hospital of Fudan UniversityNational Center for Children's HealthShanghai 201102China Department of Infectious Diseases Children's Hospital of Fudan UniversityNational Center for Children's Health.Shanghai

It has been more than 3 years since the novel coronavirus(sARs-CoV-2)pandemic raged *** coronavirus disease 2019(COVID-19)has greatly influenced human *** to data from the World Health Organization(WHO),there were ove... 详细信息

关键词： prevention infection statement

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Cellular zinc metabolism and zinc signaling:from biological functions to diseases and therapeutic targets

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signal Transduction and Targeted Therapy 2024年第2期9卷 401-441页

作者： Bonan Chen Peiyao Yu Wai Nok Chan Fuda Xie Yigan Zhang Li Liang Kam Tong Leung Kwok Wai Lo Jun Yu Gary M.K.Tse Wei Kang Ka Fai To Department of Anatomical and Cellular Pathology State Key Laboratory of Translational OncologyPrince of Wales HospitalThe Chinese University of Hong KongHong KongChina state Key Laboratory of Digestive Disease Institute of Digestive DiseaseThe Chinese University of Hong KongHong KongChina CUHK-shenzhen research institute The Chinese University of Hong KongShenzhenChina Department of Pathology Nanfang Hospital and Basic Medical CollegeSouthern Medical UniversityGuangdong Province Key Laboratory of Molecular Tumor PathologyGuangzhouChina institute of Biomedical research Taihe HospitalHubei University of MedicineShiyanChina Department of Pediatrics The Chinese University of Hong KongHong KongChina Department of Medicine and Therapeutics The Chinese University of Hong KongHong KongChina

Zinc metabolism at the cellular level is critical for many biological processes in the body.A key observation is the disruption of cellular homeostasis,often coinciding with disease *** an essential factor in maintaining cellular equilibrium,cellular zinc has been increasingly spotlighted in the context of disease *** research suggests zinc’s involvement in promoting malignancy and invasion in cancer cells,despite its low tissue *** has led to a growing body of literature investigating zinc’s cellular metabolism,particularly the functions of zinc transporters and storage mechanisms during cancer *** transportation is under the control of two major transporter families:sLC30(ZnT)for the excretion of zinc and sLC39(ZIP)for the zinc ***,the storage of this essential element is predominantly mediated by metallothioneins(MTs).This review consolidates knowledge on the critical functions of cellular zinc signaling and underscores potential molecular pathways linking zinc metabolism to disease progression,with a special focus on *** also compile a summary of clinical trials involving zinc *** the main localization of zinc transporters at the cell membrane,the potential for targeted therapies,including small molecules and monoclonal antibodies,offers promising avenues for future exploration.

关键词： metabolism invasion involvement

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Prehospital transdermal glyceryl trinitrate for ultra- acute ischaemic stroke: data from the RIGHT- 2 randomised sham- controlled ambulance trial

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stroke & Vascular Neurology 2024年第1期9卷 38-49,I0035-I0046页

作者： Jason Philip Appleton Lisa J Woodhouse Craig s Anderson sandeep Ankolekar Lesley Cala Mark Dixon Timothy J England Kailash Krishnan Grant Mair Keith W Muir John Potter Christopher I Price Marc Randall Thompson G Robinson Christine Roffe Else C sandset Jeffrey L saver Angela shone Aloysius Niroshan siriwardena Joanna M Wardlaw Nikola sprigg Philip M Bath stroke University Hospitals Birmingham NHS Foundation TrustBirminghamUK institute of Applied Healthresearch University of Birmingham College of Medical and Dental SciencesBirminghamUK stroke Trials Unit Mental Health and Clinical NeurosciencesUniversity of NottinghamNottinghamUK Faculty of Medicine The George Institute for Global HealthSydneyNew South WalesAustralia The George institute China at Peking University Health science Center BeijingChina Neurology Royal Prince Alfred HospitalSydney Health PartnersSydneyNew South WalesAustralia Department of Neurology King's College Hospital NHS Foundation TrustLondonUK Faculty of Health and medical sciences University of Western AustraliaCrawleyWestern AustraliaAustralia East Midlands Ambulance service NHs Trust NottinghamUK stroke Queen's Medical CentreNottingham University Hospitals NHS TrustNottinghamUK Centre for Clinical Brain sciences Dementia Research InstituteUniveristy of EdinburghEdinburghUK institute of Neurology and Psychology University of GlasgowGlasgowUK Bob Champion research and Education Building University of East AngliaNorwichUK institute of Neuroscience Newcastle UniversityNewcastle upon TyneUK Department of Neurology Leeds Teaching Hospitals NHS TrustLeedsUK Department of Cardiovascular sciences and NIHR Leicester Biomedical research Centre University of LeicesterLeicesterUK stroke research in stoke Institute for Science and Technology in MedicineKeele UniversityStoke-on TrentUK Department of Neurology Oslo University HospitalOsloNorway research and Development Norwegian Air Ambulance FoundationOsloNorway Department of Neurology and Comprehensive stroke Center David Geffen School of MedicineUCLALos AngelesCaliforniaUSA research and Graduate services University of NottinghamNottinghamUK Community and Health research Unit University of LincolnLincolnUK

Background The effect of transdermal glyceryl trinitrate(GTN,a nitrovasodilator)on clinical outcome when administered before hospital admission in suspected stroke patients is ***,we assess the safety and efficacy of GTN in the prespecified subgroup of patients who had an ischaemic stroke within the Rapid Intervention with Glyceryl trinitrate in Hypertensive stroke Trial-2(RIGHT-2).Methods RIGHT-2 was an ambulance-based multicentre sham-controlled blinded-endpoint study with patients randomised within 4hours of *** primary outcome was a shift in scores on the modified Rankin scale(mRs)at day *** outcomes included death;a global analysis(Wei-Lachin test)containing Barthel Index,EuroQol-5D,mRs,telephone interview for cognitive status-modified and Zung depression scale;and neuroimaging-determined‘brain frailty’*** were reported as n(%),mean(sD),median[IQR],adjusted common OR(acOR),mean difference or Mann-Whitney difference(MWD)with 95%*** 597 of 1149(52%)patients had a final diagnosis of ischaemic stroke;age 75(12)years,premorbid mRs>2107(18%),Glasgow Coma scale 14(2)and time from onset to randomisation 67[45,108]***‘brain frailty’was common:median score 2[2,3](range 0–3).At day 90,GTN did not influence the primary outcome(acOR for increased disability 1.15,95%CI 0.85 to 1.54),death or global analysis(MWD 0.00,95%CI-0.10 to 0.09).In subgroup analyses,there were non-significant interactions suggesting GTN may be associated with more death and dependency in participants randomised within 1hour of symptom onset and in those with more severe *** In patients who had an ischaemic stroke,ultra-acute administration of transdermal GTN in the ambulance did not improve clinical outcomes in a population with more clinical and radiological frailty than seen in previous in-hospital *** Is ALREADY KNOWN ON THIs TOPIC⇒Transdermal glyceryl trinitrate(GTN)was associat-ed with less death and dependency in those w

关键词： Glasgow nitrate random

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E-voting system using cloud-based hybrid blockchain technology

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Journal of safety science and Resilience 2024年第1期5卷 102-109页

作者： Beulah Jayakumari s Lilly sheeba Maya Eapen Jani Anbarasi Vinayakumar Ravi A.suganya Malathy Jawahar Department of Information Technology Tagore Engineering College Chennai India school of Computer science and Engineering sRM institute of science and Technology Ramapuram ChennaiIndia Department of Information Technology Jerusalem College of Engineering ChennaiIndia school of Computer science and Engineering Vellore institute of Technology Chennai India Center for Artificial Intelligence Prince Mohammad Bin Fahd UniversityKhobarSaudi Arabia Leather Process Technology Division CsIR-Central Leather research institute ChennaiIndia

With the invention of Internet-enabled devices,cloud and blockchain-based technologies,an online voting system can smoothly carry out election *** pandemic situations,citizens tend to develop panic about mass gatherings,which may influence the decrease in the number of *** urges a reliable,flexible,transparent,secure,and cost-effective voting *** proposed online voting system using cloud-based hybrid blockchain technology eradicates the flaws that persist in the existing voting system,and it is carried out in three phases:the registration phase,vote casting phase and vote counting phase.A timestamp-based authentication protocol with digital signature validates voters and candidates during the registration and vote casting *** smart contracts,third-party interventions are eliminated,and the transactions are secured in the blockchain ***,to provide accurate voting results,the practical Byzantine fault tolerance(PBFT)consensus mechanism is adopted to ensure that the vote has not been modified or ***,the overall performance of the proposed system is significantly better than that of the existing *** performance was analyzed based on authentication delay,vote alteration,response time,and latency.

关键词： E-voting Cloud Blockchain smart contract Consensus mechanism

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Cellular Chemical Field and Cellular Homeostasis

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Engineering 2024年第8期39卷 18-24页

作者： Bin Cong Lu Li Qian Wang Tao He Junwei Li Hongliang Xie Aolin Zhang Xiaohui Fan Hebei Key Laboratory of Forensic Medicine College of Forensic MedicineHebei Medical UniversityShijiazhuang 050017China College of Integrated Traditional Chinese and Western Medicine Hebei Medical UniversityShijiazhuang 050017China research Unit of Digestive Tract Microecosystem Pharmacology and Toxicology Chinese Academy of Medical SciencesShijiazhuang 050017China Pharmaceutical Informatics institute College of Pharmaceutical SciencesZhejiang UniversityHangzhou 310058China Key Laboratory of Chinese Medicine Modernization Innovation Center of Yangtze River DeltaZhejiang UniversityJiaxing 314100China Department of Obstetrics and Gynecology Sir Run Run Shaw HospitalMedical College of Zhejiang UniversityHangzhou 310058China Department Obstetrics and Gynaecology Prince of Wales HospitalThe Chinese University of Hong KongHong Kong 999077China

*** Cell structure and function are controlled by a complex regulatory networking process,in which molecular adaptation and interaction are essential to maintain cellular homeostasis following dynamic multi-omics rules and multi-systematic *** complex chemical process is an important mechanism for stabilizing the cell microenvironment.

关键词： maintain stabilizing Cellular

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Inhibiting the oligomerization of mycobacterial DNA-directed RNA polymerase(RNAP)using natural compound via in-silico techniques

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Medicine in Novel Technology and Devices 2024年第1期21卷 97-109页

作者： Ehssan H.Moglad Department of Pharmaceutics College of PharmacyPrince Sattam Bin Abdulaziz UniversityP.O.Box 173 Alkharj 11942Saudi Arabia Department of Microbiology and Parasitology Institute of Medicinal and Aromatic Plants ResearchThe National Center for ResearchP.O.Box 2404KhartoumSudan

Mycobacterium tuberculosis(Mtb)is responsible for the spread of tuberculosis(TB).The current study employed virtual screening of 2569 natural compounds against the DNA-directed RNA polymerase(RNAP)of Mtb to identify the possible binders that can inhibit its *** in-silico methodology included molecular docking to the compounds,further,the stability and flexibility of the best complexes were studied using molecular dynamics simulation,the MM/GBsA binding free energy technique with energy decomposition,PCA,FEL,steered MD simulation,and umbrella *** virtual screenings were conducted for the five RNAP subunits(chains A,B,C,D,and E)to identify a compound capable of inhibiting RNAP oligomerization.A promising compound,isoestradiol 3-benzoate,exhibited a low binding score(−7.28kcal/mol to−8.21kcal/mol)and showed binding ability with all subunits of the ***,the five complexes with isoestradiol 3-benzoate were selected for molecular dynamics simulation ***,RMsD showed that isoestradiol 3-benzoate bound with chain E showed the lowest RMsD of 0.49nm,while with chains A and B it had the most stable and consistent conformations with RMsD of 1.75nm and 1.2nm,*** H-bond between isoestradiol 3-benzoate and chains C and E showed the highest occupancy(58.27%,45.33%,and 50.80%,42.25%,11.75%).Moreover,the MMPBsA technique showed that isoestradiol 3-benzoate had a strong association with chains B and C(ΔGbind=−126.25±2.03 and−129.27±2.25).Additionally,free energy decomposition,PCA,FEL-steered MD simulation,and umbrella sampling were also performed to validate the association of the ligand with the *** 3-benzoate binds strongly to chains B and E;therefore,it should be considered as viable candidate for inhibiting the formation of RNAP protein complex,concluded in this study.

关键词： Mycobacterium tuberculosis DNA-Directed RNA polymerase Docking steered MD simulation Natural compounds Binding free energy Umbrella sampling

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Modelling and Characterization of Basalt/Vinyl Ester/siC Micro-and Nano-hybrid Biocomposites Properties Using Novel ANN–GA Approach

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Journal of Bionic Engineering 2024年第2期21卷 938-952页

作者： Yesudhasan Thooyavan Lakshmi Annamali Kumaraswamidhas Robinson Dhas Edwin Raj Joseph selvi Binoj Bright Brailson Mansingh Antony sagai Francis Britto Alamry Ali Department of Mechanical Engineering Indian Institute of Technology Dhanbad(ISM)DhanbadJharkhand826004India Gati shakti Vishwavidyalaya(GsV-A Central University Funded By GoI in the Ministry of Railways) LalbaugVadodaraGujarat390004India institute of Mechanical Engineering Saveetha School of EngineeringSaveetha Institute of Medical and Technical Sciences(SIMATS)Saveetha UniversityChennaiTamilnadu602105India Metal Joining research Centre Department of Mechanical EngineeringSri Ramakrishna Engineering CollegeCoimbatoreTamilnadu641022India Department of Mechanical Engineering Rohini College of Engineering and TechnologyPalkulamTamil Nadu629401India Department of Mechanical Engineering College of Engineering in Al-KharjPrince Sattam Bin Abdulaziz University11942Al-KharjSaudi Arabia

Basalt fiber reinforcement in polymer matrix composites is becoming more and more popular because of its environmental friendliness and mechanical qualities that are comparable to those of synthetic *** fiber strengthened vinyl ester matrix polymeric composite with filler addition of nano-and micro-sized silicon carbide(siC)element spanning from 2 weight percent to 10 weight percent was studied for its mechanical and wear *** application of Artificial Neural Network(ANN)to correlate the filler addition composition for optimum mechanical properties is required due to the non-linear mechanical and tribological features of *** stuffing blend and composition of the composite are optimized using the hybrid model and Genetic Algorithm(GA)to maximize the mechanical and wear-resistant *** predicted and tested ANN–GA optimal values obtained for the composite combination had a tensile,flexural,impact resilience,hardness and wear properties of 202.93 MPa,501.67 MPa,3.460 J/s,43 HV and 0.196 g,respectively,for its optimum combination of filler and *** can be noted that the nano-sized siC filler particle enhances most of the properties of the composite which diversifies its *** predicted mechanical and wear values of the developed ANN–GA model were in closer agreement with the experimental values which validate the model.

关键词： Hybrid polymer composite Prediction Process modelling Artificial neural networks Genetic algorithm

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