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An Epigenetic Role for Disrupted Paternal Gene Expression in Postzygotic Seed Abortion in Arabidopsis Interspecific Hybrids

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Molecular plant 2015年第12期8卷 1766-1775页

作者： Ryan C. Kirkbride Helen Hong Yu Gyoungju Nah Changqing Zhang Xiaoli Shi Z. Jeffrey Chen department of Molecular Biosciences Center for Computational Biology and Bioinformatics and Institute for Cellular and Molecular Biology The University of Texas at Austin Austin TX 78712 USA These authors contributed equally to this article. present address: department of plant Science Research Institute of Agriculture and Life Sciences Seoul National University Seou1151-921 South Korea present address: plants for Human Health Institute North Carolina State University 600 Laureate Way Kannapolis NC 28081 USA present address: Institute of Theoretical Physics Chinese Academy of Sciences PO Box 2735 Beijing 100080 China

Interspecific hybrids often increase the levels of heterozygosity and hybrid vigor, but some interspecific hybrid seeds are aborted shortly after fertilization. The mechanism behind this postzygotic seed abortion is poorly understood. Here, we report genome-wide analysis of allelic expression changes in developing siliques and seeds in three F1 interspecific crosses between Arabidopsis thaliana （Col, Ler, or C24） and Arabidopsis arenosa. The majority of maternally expressed genes （MEGs） were shared among all three F1 interspecific crosses, whereas ~90% of 272 paternally expressed genes （PEGs） were found only in one or two F1 crosses, suggesting a role for disrupted paternal gene expression in seed abortion that varies in different crosses. Consistent with this notion, 12 PEGs in the infertile interspecific hybrids matched MEGs in fertile intraspecific hybrids. This disruption of PEGs in the interspecific hybrids was consistent with the upregulation of the genes in the paternal-excess interploidy cross （2X6） between a diploid mother and a hexaploid father, leading to the seed abortion. Moreover, a subset of PEGs in the interspecific crosses were also upregulated in the intraspecific hybrid metlXWT or meaXWT, in which the mutant of MET1 （DNA METHYL TRANSFERASE1） or MEDEA, a Polycomb Repressive Complex2 gene, was used as the maternal parent. These data suggest that maternal epigenetic factors and paternal gene expression play important roles in the postzygotic seed abortion in interspecific hybrids or neo-allopolyploids.

关键词： epigenetics imprinting paternal gene expression polyploidy hybrid incompatibility seeddevelopment

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SIZ1-Mediated SUMO Modification of SEUSS Regulates Photomorphogenesis in Arabidopsis

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plant Communications 2020年第5期1卷 73-84页

作者： Xinyu Zhang Junling Huai Shuangrong Liu Jing Bo Jin Rongcheng Lin Key Laboratory of Photobiology Institute of BotanyChinese Academy of SciencesBeijing 100093China University of Chinese Academy of sciences Beijing 100049China Key Laboratory of plant Molecular Physiology Institute of BotanyChinese Academy of SciencesBeijing 100093China present address:department of plant sciences University of OxfordSouth Parks RoadOxford OX13RBUK

Small ubiquitin-like modifier(SUMO)post-translational modification(SUMOylation)plays essential roles in regulating various biological processes;however,its function and regulation in the plant light signaling pathway are largely unknown.SEUSS(SEU)is a transcriptional co-regulator that integrates light and temperature signaling pathways,thereby regulating plant growth and development in Arabidopsis thaliana.Here,we show that SEU is a substrate of SUMO1,and that substitution of four conserved lysine residues disrupts the SUMOylation of SEU,impairs its function in photo-and thermomorphogenesis,and enhances its interaction with PHYTOCHROME-INTERACTING FACTOR 4 transcription factors.Furthermore,the SUMO E3 ligase SIZ1 interacts with SEU and regulates its SUMOylation.Moreover,SEU directly interacts with phytochrome B photoreceptors,and the SUMOylation and stability of SEU are activated by light.Our study reveals a novel post-translational modification mechanism of SEU in which light regulates plant growth and development through SUMOylation-mediated protein stability.

关键词： light signaling phytochrome PIF4 SUMOylation

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Timing Is Everything： Highly Specific and Transient Expression of a MAP Kinase Determines Auxin-Induced Leaf Venation Patterns in Arabidopsis

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Molecular plant 2014年第11期7卷 1637-1652页

作者： Vera Stanko Concetta Giuliani Katarzyna Retzer Armin Djamei Vanessa Wahl Bernhard Wurzinger Cathal Wilson Erwin Heberle-Bors Markus Teige Friedrich Kragler department of plant Molecular Biology Max. F. Perutz Laboratories University of Vienna Dr. Bohrgasse 9/4 Vienna A-1030 Austria present address: Felix-Klein-Gymnasium Bottingerstraβe 17 D-37073 Gottingen Germany present address: Austrian Centre of Industrial Biotechnology Muthgasse 11 A-1190 Vienna Austria present address: department of Applied Genetics and Cell Biology University of Natural Resources and Life Sciences Muthgasse 18 A-1190 Vienna Austria present address: Gregor Mendel Institute of Molecular plant Biology Dr. Bohr-Gasse 3 1030 Vienna Austria Max Planck Institute of Molecular plant Physiology Wissenschaftspark Golm Am Muhlenberg 1 D-14476 Potsdam-Golm Germany department of Biochemistry Max. F. Perutz Laboratories University of Vienna Dr. Bohrgasse 9/5 Vienna A-1030 Austria present address: department of Ecogenomics and Systems Biology University of Vienna Althanstr. 14 A-1090 Vienna Austria present address: Telethon Institute of Genetics and Medicine Via Pietro Castellino 11180131-Naples Italy department of Ecogenomics and Systems Biology University of Vienna AIthanstr. 14 A-1090 Vienna Austria

Mitogen-activated protein kinase （MAPK） cascades are universal signal transduction modules present in all eukaryotes. In plants, MAPK cascades were shown to regulate cell division, developmental processes, stress responses, and hormone pathways. The subgroup A of Arabidopsis MAPKs consists of AtMPK3, AtMPK6, and AtMPK10. AtMPK3 and AtMPK6 are activated by their upstream MAP kinase kinases （MKKs） AtMKK4 and AtMKK5 in response to biotic and abiotic stress. In addition, they were identified as key regulators of stomatal development and patterning. AtMPKIO has long been considered as a pseudo-gene, derived from a gene duplication of AtMPK6. Here we show that AtMPKIO is expressed highly but very transiently in seedlings and at sites of local auxin maxima leaves. MPK10 encodes a functional kinase and interacts with the upstream MAP kinase kinase （MAPKK） AtMKK2. mpklO mutants are delayed in flowering in long-day conditions and in continuous light. Moreover, cotyledons of mpk10 and mkk2 mutants have reduced vein complexity, which can be reversed by inhibiting polar auxin transport （PAT）. Auxin does not affect AtMPKIO expression while treatment with the PAT inhibitor HFCA extends the expression in leaves and reverses the mpklO mutant phenotype. These results suggest that the AtMKK2-AtMPK10 MAPK module regulates venation complexity by altering PAT efficiency.

关键词： Arabidopsis MAP kinase leaf development polar auxin transport leaf venation pattern.

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Nutrient enrichment driven by canopy rainfall redistribution:Mechanism,quantification,and pattern

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Science China Earth sciences 2024年第5期67卷 1529-1544页

作者： Chuan YUAN Xiaoping YUE Yafeng ZHANG Yu ZHANG Yanting HU Qiang TANG Li GUO Shuai WANG Xingwu DUAN Wenhua XIANG Xiaohua WEI Xiaoyan LI College of Forestry and Biotechnology Zhejiang A&F UniversityHangzhou 311300China Chongqing Jinfo Mountain Karst Ecosystem National Observation and Research Station School of Geographical SciencesSouthwest UniversityChongqing 400715China Shapotou Desert Research and Experiment Station Northwest Institute of Eco-Environment and ResourcesChinese Academy of SciencesLanzhou 730000China School of Geographical sciences Hebei Normal UniversityShijiazhuang 050024China Faculty of Life Science and Technology Central South University of Forestry and TechnologyChangsha 410004China State Key Laboratory of Hydraulics and Mountain River Engineering College of Water Resource and HydropowerSichuan UniversityChengdu 610065China State Key Laboratory of Earth Surface Processes and Resource Ecology Faculty of Geographical ScienceBeijing Normal UniversityBeijing 100875China Yunnan Key Laboratory of Soil Erosion Prevention and Green Development Institute of International Rivers and Eco-securityYunnan UniversityKunming 650091China department of Earth Environmental and Geographic SciencesUniversity of British Columbia(Okanagan)KelownaBritish Columbia V1V 1V7Canada

Vegetation canopies intercept and redistribute rainfall into throughfall and stemflow,which transfer substantial amounts of elements into the soil,influencing soil microbial community,plant survival,and plant community succession.Despite advancements in ecohydrological research,the implication of nutrient enrichment resulting from this redistribution of rainfall by canopies remains largely unexplored.To address this gap,we conducted a systematic review of 1020 papers published between 2000 and 2022,gathering data on nutrient concentration and enrichment for critical ions(including K^(+),Na^(+),Ca^(2+),Mg^(2+),NH_(4)^(+),Cl^(-),NO_(3)^(-)and SO_(4)^(2-))from the Web of Science and Chinese Knowledge Infrastructure databases.We aimed to synthesize the mechanisms,quantify the enrichments,and identify global patterns of nutrient enrichment in stemflow and throughfall across climate zones,and vegetation types and ecosystems.The results of this study indicate that stemflow exhibits,on average,2.1times greater ion concentration(6.13 mg L^(-1))compared to throughfall.In particular,among the investigated ions,SO_(4)^(2-)(12.45and 6.32 mg L^(-1))for stemflow and throughfall,respectively,and Cl^(-)(9.21 and 4.81 mg L^(-1))exhibit the highest concentrations in both rainfall redistribution components,while K^(+)(13.7 and 5.8)and Mg^(2+)(5.6 and 2.8)have the highest enrichment factors.Across climate zones,throughfall and stemflow show the lowest ion concentrations but the highest enrichment factors in extremely humid regions.Along the temperature gradient,ion concentrations are the highest in cold climates with no clear patterns observed for enrichment factors with increasing temperature.In addition,shrubs,conifers,mixed forests,and artificial ecosystems demonstrate enrichment factors 1.1 to 3.0 times greater than those of trees,broad-leaved plants,pure forests,and natural ecosystems.These findings emphasize the need for increased attentions to artificial ecosystems,such as urban and agricultural ecosystems,which often received

关键词： Ecohydrology Rainfall redistribution Nutrients transport Enrichment characteristics Climate zones

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From Squalene to Brassinolide： The Steroid Metabolic and Signaling Pathways across the plant Kingdom

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Molecular plant 2013年第6期6卷 1738-1757页

作者： Cdcile Vriet Eugenia Russinova Christophe Reuzeau CropDesign NV a BASF Plant Science Company 9052 Gent Belgium present address: Laboratoire de G~n~tique et-de Biophysique c/es plantes Aix-Marseille UniversitY Marseille F-13009 France present address: Centre National de la Recherche Scientifique Unite Mixte de Recherche Biologie Vc~gEtale et de Microbiologie Environmentale MarseilleF-13009 France present address: Commissariat TEnergie Atomique Direction des Sciences du Vivant Institut de Biologie Environnementale et Biotechnologie MarseilleF-13009 France department of plant Systems Biology VIB 9052 Gent Belgium department of plant Biotechnology and Bioinformatics Ghent University 9052 Gent Belgium

The plant steroid hormones, brassinosteroids （BRs）, and their precursors, phytosterols, play major roles in plant growth, development, and stress tolerance. Here, we review the impressive progress made during recent years in elucidating the components of the sterol and BR metabolic and signaling pathways, and in understanding their mecha- nism of action in both model plants and crops, such as Arabidopsis and rice. We also discuss emerging insights into the regulations of these pathways, their interactions with other hormonal pathways and multiple environmental signals, and the putative nature of sterols as signaling molecules.

关键词： Arabidopsis rice phytosterols brassinosteroids.

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The Formation of Anthocyanic Vacuolar Inclusions in Arabidopsis thaliana and Implications for the Sequestration of Anthocyanin Pigments

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Molecular plant 2010年第1期3卷 78-90页

作者： Lucille Pourcel Niloufer G. Irani Yuhua LU Ken Riedl Steve Schwartz Erich Grotewold department of plant Cellular and Molecular Biology and plant Biotechnology Center Ohio State University Columbus OH 43210 USA department of Food Science and Technology The Ohio State University Columbus OH 43210 USA present address: department of plant Systems Biology Flanders Institute for Biotechnology and Department of Plant Biotechnology and Genetics GhentUniversity 9052 Ghent Belgium Current address: department of Chemistry Eastern Illinois University Charleston IL 61920 USA

Anthocyanins are flavonoid pigments that accumulate in the large central vacuole of most plants. Inside the vacuole, anthocyanins can be found uniformly distributed or as part of sub-vacuolar pigment bodies, the Anthocyanic Vacuolar Inclusions （AVIs）. Using Arabidopsis seedlings grown under anthocyanin-inductive conditions as a model to un- derstand how AVIs are formed, we show here that the accumulation of AVIs strongly correlates with the formation of cyanidin 3-glucoside （C3G） and derivatives. Arabidopsis mutants that fail to glycosylate anthocyanidins at the 5-0 position （Sgt mutant） accumulate AVIs in almost every epidermal cell of the cotyledons, as compared to wild-type seedlings, where only a small fraction of the cells show AVIs. A similar phenomenon is observed when seedlings are treated with vanadate. Highlighting a role for autophagy in the formation of the AVIs, we show that various mutants that interfere with the autophagic process （atg mutants） display lower numbers of AVIs, in addition to a reduced accumulation of anthocyanins. Interestingly, vanadate increases the numbers of AVIs in the atg mutants, suggesting that several pathways might participate in AVl formation. Taken together, our results suggest novel mechanisms for the formation of sub-vacuolar compartments capable of accumulating anthocyanin pigments.

关键词： Anthocyanin autophagy cyanidin 3-glucoside vacuolar inclusion vanadate.

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Neuronal architecture and functional mapping of the taste center of larval Helicoverpa armigera(Lepidoptera:Noctuidae)

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Insect Science 2022年第3期29卷 730-748页

作者： Long-Long Sun Xiao-Lan Liu Ya-Nan Wang Bente G.Berg Gui-Ying Xie Wen-Bo Chen Yang Liu Gui-Rong Wang Xin-Cheng Zhao Qing-Bo Tang department of Entomology College of Plant ProtectionHenan Agricultural UniversityZhengzhou450002China Chemosensory laboratory Department of PsychologyNorwegian University of Science and TechnologyTrondheim7489Norway State Key Laboratory for Biology of plant Disease and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing100193China

The sense of taste plays a crucial role in herbivorous insects by discriminating nutrients from complex plant metabolic compounds.The peripheral coding of taste has been thoroughly studied in many insect species,but the central gustatory pathways are poorly described.In the present study,we characterized single neurons in the gnathal ganglion of Helicoverpa armigera larvae using the intracellular recording/staining technique.We identified different types of neurons,including sensory neurons,interneurons,and motor neurons.The morphologies of these neurons were largely diverse and their arborizations seemingly covered the whole gnathal ganglion.The representation of the single neurons responding to the relevant stimuli of sweet and bitter cues showed no distinct patterns in the gnathal ganglion.We postulate that taste signals may be processed in a manner consistent with the principle of population coding in the gnathal ganglion of H.armigera larvae.

关键词： gnathal ganglion interneuron intracellular recording and staining motor neuron sensory neuron taste coding model

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Plasma Membrane Localization and Potential Endocytosis of Constitutively Expressed XA21 Proteins in Transgenic Rice

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Molecular plant 2010年第5期3卷 917-926页

作者： Fang Chen Ming-Jun Gao Yan-Song Miao Yue-Xing Yuan Mu-Yang Wang Qun Li Bi-Zeng Mao Li-Wen Jiang Zu-Hua He National Key Laboratory of plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China present address: department of Molecular Cellular and Developmental Biology Yale University New Haven CT 06511 USA department of Biology and Molecular Biotechnology Program The Chinese University of Hong Kong Shatin New Territories Hong Kong China present address: department of Emergency Medicine Thomas Jefferson University Philadelphia PA 19107 USA Biotechnology Institute Zhejiang University Hanghzou 310029 China

The rice pattern recognition receptor （PRR） XA21 confers race-specific resistance in leaf infection by bacterial blight Xathornonas oryzae pv. oryzae （Xoo）, and was shown to be primarily localized to the endoplasmic reticulum （ER） when expressed with its native promoter or overexpressed in the protoplast. However, whether the protein is still ER- localization in the intact cell when overexpressed remains to be identified. Here, we showed that XA21, its kinase-dead mutant XA21PK736EP and the triple autophosphorylation mutant XA21PS686AJT688AJS699A GFP fusions were primarily localized to the plasma membrane （PM） when overexpressed in the intact transgenic rice cell, and also localized to the ER in the transgenic protoplast. The transgenic plants constitutively expressing the wild-type XA21 or its GFP fusion displayed racespecific resistance to Xoo at the adult and seedling stages. XA21 and XA21PK736EP could be internalized probably via the SCAMP-positive early endosomal compartment in the protoplast, suggesting that XA21 might be endocytosed to initiate resistance responses during pathogen infection. We also established a root infection system and demonstrated that XA21 also mediated race-specific resistance responses to Xoo in the root. Our current study provides an insight into the nature of the XA21-mediated resistance and a practical approach using the root cell system to further dissect the cellular signaling of the PRR during the rice-Xoo interaction.

关键词： Rice XA21 pattern recognition receptor bacterial bight cellular localization internalization root infection race-specific resistance.

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Dissecting Abscisic Acid Signaling Pathways Involved in Cuticle Formation

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Molecular plant 2016年第6期9卷 926-938页

作者： Fuqiang Cui Mikael Brosche Mikko T. Lehtonen Ali Amiryousefi Enjun Xu Matleena Punkkinen Jari P.T. Valkonen Hiroaki Fujii Kirk Overmyer Division of plant Biology Viikki Plant Science Centre Department of Biosciences University of Helsinki 00014 Helsinki Finland department of Agricultural sciences University of Helsinki PO Box 27 00014 Helsinki Finland department of Biochemistry Molecular Plant Biology University of Turku Turku 20014 Finland Institute of Technology University of Tartu Nooruse 1 Tartu 50411 Estonia present address: Institute of Botany The Chinese Academy of Sciences Beijing China

The cuticle is the outer physical barrier of aerial plant surfaces and an important interaction point between plants and the environment. Many environmental stresses affect cuticle formation, yet the regulatory pathways involved remain undefined. We used a genetics and gene expression analysis in Arabidopsis thaliana to define an abscisic acid （ABA） signaling loop that positively regulates cuticle formation via the core ABA signaling pathway, including the PYR/PYL receptors, PP2C phosphatase, and SNF1-Related Protein Kinase （SnRK） 2.21SnRK2.3/SnRK2.6. Downstream of the SnRK2 kinases, cuticle formation was not regulated by the ABA-responsive element-binding transcription factors but rather by DEWAX, MYB16, MYB94, and MYB96. Additionally, low air humidity increased cuticle formation independent of the core ABA pathway and cell death/reactive oxygen species signaling attenuated expression of cuticle-biosynthesis genes. In Physcornitrella patens, exogenous ABA suppressed expression of cuticle- related genes, whose Arabidopsis orthologs were ABA-induced. Hence, the mechanisms regulating cuticle formation are conserved but sophisticated in land plants. Signaling specifically related to cuticle deficiency was identified to play a major role in the adaptation of ABA signaling pathway mutants to increased humidity and in modulating their immunity to Botrytis cinerea in Arabidopsis. These results define a cuticle-specific downstream branch in the ABA signaling pathway that regulates responses to the external environment.

关键词： cuticle abscisic acid Arabidopsis Physcomitrella Botrytis land plants

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plant diversity of Hyrcanian relict forests:An annotated checklist,chorology and threat categories of endemic and near endemic vascular plant species

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plant Diversity 2022年第1期44卷 39-69页

作者： Atefeh Ghorbanalizadeh Hossein Akhani Halophytes and C4 plants Research Laboratory Department of Plant SciencesSchool of BiologyCollege of SciencesUniversity of TehranP.O.Box 14155-6455TehranIran

In this paper a critical annotated checklist of 256 endemic and near endemic species belonging to 152 genera and 50 families of flowering plants known from Hyrcanian relict forests is presented.Distribution maps of taxa,elevational range,number of known records,chorotypes,life forms,IUCN threat categories and habitat types are also provided.The chorotypes are categorized into eight main patterns:1)the Omni-Hyrcanian pattern(OH),2)West Hyrcanian pattern(WH),3)Manjil-Rudbar pattern(MR),4)Central Hyrcanian pattern(CH),5)Central and East Hyrcanian pattern(CEH),6)East Hyrcanian pattern(EH),7)Alborz-Hyrcanian pattern(AH),and 8)Euxino-Hyrcanian pattern(XH).The richness and distribution maps were generated based on 5408 records gained from herbarium specimens and literature records.The life form spectra show that the majority of taxa(54.7%)belong to hemicryptophytes,followed by the tuberous,bulbous and parasitic geophytes with 45 species(17.6%)and phanerophytes with 28 taxa(10.9%).The conservation status of species according to IUCN criteria indicates that 30 taxa are Critically Endangered,52 taxa Endangered,30 taxa Vulnerable,25 taxa Near Threatened and 81 taxa are of Least Concern.Our present data were not sufficient to evaluate 38 taxa that are categorized here as Data Deficient.The new combination of Leutea translucens(=Peucedanum translucens)is validated with inclusion of Peucedanum hyrcanicum as its synonym.The disjunct occurrence of the Caucasian species Gentiana grossheimii is reported from the eastern parts of the Hyrcanian forests in Iran for the first time.We conclude that(i)the Hyrcanian forests and associated habitats in the northern slopes of the Alborz Mountains harbour tremendous floristic diversity of high conservation priority,and(ii)the Hyrcanian forest zone is an important and unique center of endemism within the Euro-Siberian region that should be considered a floristic province with a large number of relict species.

关键词： Biodiversity Distribution pattern Endemic plant Hyrcanian forests IUCN category

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