检索结果-南通市图书馆

Improved Growth and Stress Tolerance in the Arabidopsis oxtl Mutant Triggered by Altered Adenine Metabolism

Molecular Plant 2012年第6期5卷 1310-1332页

作者： Suchada Sukrong Kil-Young Yun Patrizia Stadler Charan Kumar Tony Facciuolo Barbara A.Moffatt Deane L.Falconec department of Plant and Soil Science University of KentuckyLexingtonKY 40546USA present address:department of Pharmacognosy Faculty of Pharmaceutical SciencesChulalongkorn UniversityBangkokThailand department of Biological Sciences University of MassachusettsOne University AvenueLowellMA 01854USA present address:Fungi and Plant Co.Ltd Chungcheongbuk-doRepublic of Korea department of Biology University of WaterlooWaterlooOntarioCanada

Plants perceive and respond to environmental stresses with complex mechanisms that are often associ- ated with the activation of antioxidant defenses. A genetic screen aimed at isolating oxidative stress-tolerant lines of Arabidopsis thaliana has identified oxtl, a line that exhibits improved tolerance to oxidative stress and elevated temperature but displays no apparent deleterious growth effects under non-stress conditions. Oxtl harbors a muta- tion that arises from the altered expression of a gene encoding adenine phosphoribosyltransferase （APT1）, an enzyme that converts adenine to adenosine monophosphate （AMP）, indicating a link between purine metabolism, whole-plant growth responses, and stress acclimation. The oxtl mutation results in decreased APT1 expression that leads to reduced enzymatic activity. Correspondingly, oxtl plants possess elevated levels of adenine. Decreased APT enzyme activity dir- ectly correlates with stress resistance in transgenic lines that ectopically express APT1. The metabolic alteration in oxtl plants also alters the expression of several antioxidant defense genes and the response of these genes to oxidative chal- lenge. Finally, it is shown that manipulation of adenine levels can induce stress tolerance to wild-type plants. Collectively, these results show that alterations in cellular adenine levels can trigger stress tolerance and improve growth, leading to increases in plant biomass. The results also suggest that adenine might play a part in the signals that modulate responses to abiotic stress and plant growth.

关键词： abiotic/oxidative environmental stress acclimation priming purine signaling salvage pathway thermotolerance.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Inhibition of the Ubiquitin-Proteasome Pathway Alters Cellular Levels of Nitric Oxide in Tomato Seedlings

引用

Molecular Plant 2010年第5期3卷 854-869页

作者： Sangeeta Negi Parankusam Santisree Eros Vasil Kharshiing Rameshwar Sharma School of Life Sciences University of Hyderabad Hyderabad-500 046 India present address: Donald Danforth Plant Science Centre 975 N. Warson Rd St Louis MO 63132 USA present address: St Edmunds College Shillong Meghalaya India

Nitric oxide （NO） is involved in diverse plant growth processes; however, little is known about pathways regulating NO levels in plants. In this study, we isolated a NO-overproducing mutant of tomato （Solanum lycopersicurn） in which hyper-accumulation of NO, associated with increase in nitric oxide synthase （NOS）-Iike activity, caused diminished vegetative growth of plants and showed delayed flowering. The hyper-accumulation of NO caused drastic shortening of primary root （shr） in the seedlings, while the scavenging of NO restored root elongation in shr mutant. Inhibition of NOS- like activity reduced NO levels and stimulated root elongation in the shr mutant seedlings, while inhibition of nitrate reductase （NR） activity could not rescue shr phenotype. The stimulation of NO levels in shr mutant also conferred increased resistance to pathogen Pseudomonas syringae. Application of pharmacological inhibitors regulating ubiquitin-proteasome pathway reduced NO levels and NOS-like activity and stimulated shr root elongation. Our data indicate that a signaling pathway involving regulated protein degradation likely regulates NO synthesis in tomato.

关键词： Nitric oxide tomato nitric oxide synthase short root mutant plant development.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Application of CRISPR/Cas12i.3 for targeted mutagenesis in broomcorn millet(Panicum miliaceum L.)

引用

Journal of Integrative Plant Biology 2024年第8期66卷 1544-1547页

作者： Yuhe Bai Shengnan Liu Yan Bai Zhisong Xu Hainan Zhao Haiming Zhao Jinsheng Lai Ya Liu Weibin Song State Key Laboratory of Maize Bio-breeding Key Laboratory of Genome Editing Research and ApplicationMinistry of Agriculture and Rural AffairsDepartment of Plant Genetics and BreedingNational Maize Improvement CenterCollege of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193China National Agricultural Technology Extension&Service Center Beijing 100125China Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding Beijing Academy of Agriculture and Forestry SciencesBeijing 100097China

As the global human population continues to grow and is predicted to reach 10 billion by 2050,the demand for food volume and quality raises a tremendous challenge(OECD/FAO,2017).The worldwide arable land area is decre... 详细信息

关键词： arable continue raise

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Histone Lysine Methyltransferase SDG8 Is Involved in Brassinosteroid-Regulated Gene Expression in Arabidopsis thaliana

引用

Molecular Plant 2014年第8期7卷 1303-1315页

作者： Xiaolei Wang Jiani Chen Zhouli Xie Sanzhen Liu Trevor Nolan Huaxun Ye Mingcai Zhang Hongqing Guo Patrick S. Schnable Zhaohu Li Yanhai Yin State Key Laboratory of Plant Physiology and Biochemistry Department of Agronomy College of Agronomy and Biotechnology ChinaAgricultural University Beijing 100193 PR China department of genetics Development and Cell Biology Iowa State University Ames IA 50011 USA department of Agronomy Iowa State University Ames IA 50011 USA present address: department of Plant Pathology Kansas State University Manhattan KS 66506-5502 USA present address: Du Pont Pioneer Inc. Johnston IA USA Data2Bio LLC Ames IA 50011-3650 USA

The plant steroid hormones, brassinosteroids （BRs）, play important roles in plant growth, development, and responses to environmental stresses. BRs signal through receptors localized to the plasma membrane and other signaling components to regulate the BES1/BZR1 family of transcription factors, which modulates the expression of thousands of genes. How BESl/BZR1 and their interacting proteins function to regulate the large number of genes are not com- pletely understood. Here we report that histone lysine methyltransferase SDG8, implicated in histone 3 lysine 36 diand trimethylation （H3K36me2 and me3）, is involved in BR-regulated gene expression. BES1 interacts with SDG8, directly or indirectly through IWSl, a transcription elongation factor involved in BR-regulated gene expression. The knockout mutant sdg8 displays a reduced growth phenotype with compromised BR responses. Global gene expression studies demonstrated that, while BR regulates about 5000 genes in wild-type plants, the hormone regulates fewer than 700 genes in sdg8 mutant. In addition, more than half of BR-regulated genes are differentially affected in sdg8 mutant. A Chromatin Immunoprecipitation （CHIP） experiment showed that H3K36me3 is reduced in BR-regulated genes in the sdg8 mutant. Based on these results, we propose that SDG8 plays an essential role in mediating BR-regulated gene expression. Our results thus reveal a major mechanism by which histone modifications dictate hormonal regulation of gene expression.

关键词： brassinosteroid histone modifications gene regulation.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The Formation of Anthocyanic Vacuolar Inclusions in Arabidopsis thaliana and Implications for the Sequestration of Anthocyanin Pigments

引用

Molecular Plant 2010年第1期3卷 78-90页

作者： Lucille Pourcel Niloufer G. Irani Yuhua LU Ken Riedl Steve Schwartz Erich Grotewold department of Plant Cellular and Molecular Biology and Plant Biotechnology Center Ohio State University Columbus OH 43210 USA department of Food Science and Technology The Ohio State University Columbus OH 43210 USA present address: department of Plant Systems Biology Flanders Institute for Biotechnology and Department of Plant Biotechnology and Genetics GhentUniversity 9052 Ghent Belgium Current address: department of Chemistry Eastern Illinois University Charleston IL 61920 USA

Anthocyanins are flavonoid pigments that accumulate in the large central vacuole of most plants. Inside the vacuole, anthocyanins can be found uniformly distributed or as part of sub-vacuolar pigment bodies, the Anthocyanic Vacuolar Inclusions （AVIs）. Using Arabidopsis seedlings grown under anthocyanin-inductive conditions as a model to un- derstand how AVIs are formed, we show here that the accumulation of AVIs strongly correlates with the formation of cyanidin 3-glucoside （C3G） and derivatives. Arabidopsis mutants that fail to glycosylate anthocyanidins at the 5-0 position （Sgt mutant） accumulate AVIs in almost every epidermal cell of the cotyledons, as compared to wild-type seedlings, where only a small fraction of the cells show AVIs. A similar phenomenon is observed when seedlings are treated with vanadate. Highlighting a role for autophagy in the formation of the AVIs, we show that various mutants that interfere with the autophagic process （atg mutants） display lower numbers of AVIs, in addition to a reduced accumulation of anthocyanins. Interestingly, vanadate increases the numbers of AVIs in the atg mutants, suggesting that several pathways might participate in AVl formation. Taken together, our results suggest novel mechanisms for the formation of sub-vacuolar compartments capable of accumulating anthocyanin pigments.

关键词： Anthocyanin autophagy cyanidin 3-glucoside vacuolar inclusion vanadate.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

CD36 promotes tubular ferroptosis by regulating the ubiquitination of FSP1 in acute kidney injury

引用

Genes & Diseases 2024年第1期11卷 449-463页

作者： Yixin Ma Lili Huang Zheng Zhang Pengfei Yang Qingsong Chen Xujia Zeng Fangyan Tan Chunxia Wang Xiongzhong Ruan Xiaohui Liao department of Nephrology The Second Affiliated HospitalChongqing Medical UniversityChongqing 400010China department of Cell Biology and genetics Chongqing Medical UniversityChongqing 400016China Centre for Nephrology Royal Free and University College Medical SchoolUniversity College LondonRoyal Free CampusRowland Hill StreetLondon NW32PFUnited Kingdom Centre for Lipid Research Key Laboratory of Molecular Biology on Infectious DiseasesMinistry of EducationChongqing Medical UniversityChongqing 400016China Kuanren Laboratory of Translational Lipidology Centre for Lipid ResearchSecond Affiliated Hospital of Chongqing Medical UniversityChongqing 400010China

Reactive oxidative species(ROos)production-driven ferroptosis plays a role in acute kidney injury(Akl).However,its exact molecular mechanism is poorly *** receptor CD36 has important roles in oxidizing lipids,lipid accumulation,metabolic syndrome,and insulin resistance in chronic kidney disease,but its roles remain unexplored in *** present study investigated the role and mechanism of CD36 in regulating proximal tubular cell ferroptosis and *** expression of CD36 was found to be significantly up-regulated in AKI renal tissues and correlated with renal function,which might serve as an independent biomarker for AKl ***,in adult mice subjected to AKl,deletion of CD36(CD36-/-)induced tubular cell Ros accumulation,ferroptosis activation,and renal ***,combining LC-MS/MS,co-IP,and ubiquitination analyses revealed that CD36 could specifically bind to ferroptosis suppressor protein 1(FSP1)and regulate its ubiquitination at sites K16 and K24,leading to FSP1 degradation and progression of ferroptosis in *** present results emphasize a novel mechanism of CD36 in cisplatin-induced *** discovery of the special CD36 roles in promoting ferroptosis and AKI development by regulating the ubiquitination of FSP1 in proximal tubular cells may be potential therapeutic targets for ***,CD36 may play a key role in the progression of ***,targeting CD36 may provide a promising treatment option for AKI.

关键词： Acute kidney injury CD36 Ferroptosis FSP1 Ubiquitin-dependent degradation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Translational Regulation of Metabolic Dynamics during Effector-Triggered Immunity

引用

Molecular Plant 2020年第1期13卷 88-98页

作者： Heejin Yoo George H.Greene Meng Yuan Guoyong Xu Derek Burton Lijing Liu Jorge Marques Xinnian Dong Howard Hughes Medical Institute Department of BiologyDuke UniversityBox 90338DurhamNC 27708USA National Key Laboratory of Crop Genetic Improvement National Centre of Plant Gene Research(Wuhan)Huazhong Agricultural University430070 WuhanChina present address:department of Plant Biology Ecologyand EvolutionOklahoma State UniversityStillwaterOK 74078USA present address:National Key Laboratory of Hybrid Rice Institute for Advanced Studies(IAS)Wuhan UniversityWuhanHubei 430072China present address:Key Laboratory of Plant Development and Environment Adaptation Biology Ministry of EducationSchool of Life SciencesShandong UniversityQingdaoChina present address:David H.Murdock Research Institute KannapolisNC 28081USA

Recent studies have shown that global translational reprogramming is an early activation event in pattern-triggered immunity,when plants recognize microbe-associated molecular ***,it is not fully known whether translational regulation also occurs in subsequent immune responses,such as effector-triggered immunity(ETI).In this study,we performed genome-wide ribosome profiling in Arabidopsis upon RPS2-mediated ETI activation and discovered that specific groups of genes were translationally regulated,mostly in coordination with *** genes encode enzymes involved in aromatic amino acid,phenylpropanoid,camalexin,and sphingolipid *** functional significance of these components in ETI was confirmed by genetic and biochemical *** findings provide new insights into diverse translational regulation of plant immune responses and demonstrate that translational coordination of metabolic gene expression is an important strategy for ETI.

关键词： translational regulation ribosome profiling effector-triggered immunity,ETI phenylalanine phenylpropanoids helper receptors

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Conservation and Diversification of the SHR-SCR- SCL23 Regulatory Network in the Development of the Functional Endodermis in Arabidopsis Shoots

引用

Molecular Plant 2016年第8期9卷 1197-1209页

作者： Eun Kyung Yoon Souvik Dhar Mi-Hyun Lee Jae Hyo Song Shin Ae Lee Gyuree Kim Sejeong Jang Ji Won Choi Jeong-Eun Choe Jeong Hoe Kim Myeong Min Lee Jun Lim department of Systems Biotechnology Konkuk University Seoul 05029 Korea department of Systems Biology Yonsei University Seoul 03722 Korea department of Biology Kyungpook National University Daegu 41566 Korea 4TheSe tlRors cotribCited equally/to ttats article present address: department of Agricultural Biotechnology National Institute of Agricultural Sciences Jeonju 54874 Korea present address: department of Agricultural Biology National Institute of Agricultural Sciences Wanju 55365 Korea

Development of the functional endodermis of Arabidopsis thaliana roots is controlled, in part, by GRAS transcription factors, namely SHORT-ROOT （SHR）, SCARECROW （SCR）, and SCARECROW-LIKE 23 （SCL23）. Recently, it has been shown that the SHR-SCR-SCL23 regulatory module is also essential for spec- ification of the endodermis （known as the bundle sheath） in leaves. Nevertheless, compared with what is known about the role of the SHR-SCR-SCL23 regulatory network in roots, the molecular interactions of SHR, SCR, and SCL23 are much less understood in shoots. Here, we show that SHR forms protein com- plexes with SCL23 to regulate transcription of SCL23 in shoots, similar to the regulation mode of SCR expression. Our results indicate that SHR acts as master regulator to directly activate the expression of SCR and SCL23. In the SHR-SCR-SCL23 network, we found a previously uncharacterized negative feed- back loop whereby SCL23 modulates SHR levels. Through molecular, genetic, physiological, and morpho- logical analyses, we also reveal that the SHR-SCR-SCL23 module plays a key role in the formation of the endodermis （known as the starch sheath） in hypocotyls. Taken together, our results provide new insights into the regulatory role of the SHR-SCR-SCL23 network in the endodermis development in both roots and shoots.

关键词： endodermis gene regulatory network SHORT-ROOT SCARECROW SCARECROW-LIKE 23,transcription factor

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Induction of ischemic tolerance as a promising treatment against diabetic retinopathy

引用

Neural Regeneration Research 2014年第17期9卷 1581-1584页

作者： Ruth E.Rosenstein Diego C.Fernandez Laboratory of Retinal Neurochemistry and Experimental Ophthalmology Department of Human BiochemistrySchool of Medicine/CEFyBOUniversity of Buenos Aires/CONICET present address:Johns Hopkins University Department of Biology

Diabetic retinopathy is a leading cause of acquired blindness, and it is the most common ischemic disorder of the retina. Available treatments are not very effective. Efforts to inhibit diabetic reti- nopathy have focused either on highly specific therapeutic approaches for pharmacologic targets or using genetic approaches to change expression of certain enzymes. However, it might be wise to choose innovative treatment modalities that act by multiple potential mechanisms. The resis- tance to ischemic injury, or ischemic tolerance, can be transiently induced by prior exposure to a non-injurious preconditioning stimulus. A complete functional and histologic protection against retinal ischemic damage can be achieved by previous preconditioning with non-damaging isch- emia. In this review, we will discuss evidence that supports that ischemic conditioning could help avert the dreaded consequences that results from retinal diabetic damage.

关键词： diabetic retinopathy ischemic tolerance retina ischemic injury ischemic conditioning

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Arabidopsis Extra Large G-Protein 2 （XLG2） Interacts with the Gβ Subunit of Heterotrimeric G Protein and Functions in Disease Resistance

引用

Molecular Plant 2009年第3期2卷 513-525页

作者： Huifen Zhu Guo-Jing Li Lei Ding Xiangqin Cui Howard Berg Sarah M. Assmann Yiji Xia Donald Danforth Plant Science Center St Louis MO 63132 USA present address: College of Bioengineering Inner Mongolia Agricultural University Hohhot Inner Mongolia 010018 China Biology department Penn State University University Park PA 16802 USA present address: department of Biology Indiana University Bloomington IN 47405 USA department of Biostatistics University of Alabama at Birmingham Birmingham AL 35294 USA

Heterotrimeric GTP-binding proteins, which consist of Gα, Gβ, and Gγ subunits, play important roles in transducing extracellular signals perceived by cell surface receptors into intracellular physiological responses. In addition to a single prototypical Gα protein （GPA1）, Arabidopsis has three unique Gα-Iike proteins, known as XLG1, XLG2, and XLG3, that have been found to be localized in nuclei, although their functions and mode of action remain largely unknown. Through a transcriptomic analysis, we found that XLG2 and XLG3 were rapidly induced by infection with the bacterial pathogen Pseudomonas syringae, whereas the XLG1 transcript level was not affected by pathogen infection. A reverse genetic screen revealed that the xlg2 loss-of-function mutation causes enhanced susceptibility to P. syringae. Transcriptome profiling revealed that the xlg2 mutation affects pathogen-triggered induction of a small set of defense-related genes. However, xlgl and xlg3 mutants showed no difference from wild-type plants in resistance to P. syringae, In addition, the xlg2 xlg3 double mutant and the xlgl xlg2 xlg3 triple mutant were not significantly different from the xlg2 single mutant in the disease resistance phenotype, suggesting that the roles of XLG1 and XLG3 in defense, if any, are less significant than for XLG2. Constitutive overexpression of XLG2 leads to the accumulation of abnormal transcripts from multiple defense-related genes. Through co-immunoprecipitation assays, XLG2 was found to interact with AGB1, the sole Gβ subunit in Arabidopsis, which has previously been found to be a positive regulator in resistance to necrotrophic fungal pathogens. However, no significant difference was found between three xlg single mutants, the xlg2 xlg3 double mutant, the xlgtriple mutant, and wild-type plants in resistance to the necrotrophic fungal pathogens Botrytis cinerea or Alternaria brassicicola. These results suggest that XLG2 and AGB1 are components of a G-protein complex different

关键词： Defense responses disease resistance plant-microbe interactions Arabidopsis G-protein.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：